It works. hahha
the problem is very funny.
cause I set autonumber for my table records, and later i deleted the first
five records, so the autonumbre begins from 6, that is why i cannot get the
value shown up. because my option value passed to asp begins from 1 to 3.
whatever. thanks all.
btw, why do I always make this kind of stupic mistake. too careless.
在 huster (huster) 的大作中提到: 】
Usually, it will take 3 times longer time for debugging and maintaining the
code than writting the code. Which will let programmers always have work to do
number.
1. Click Start and then Run
2. Type "regedit", then click OK
3. In the "Registry Editor" window, pick Find from Edit pulldown menu
4. In the pop up "Find" dialog box, type "LMMIB20.DLL" , click "Find Next" to
find the relevant entry, and delete it.
5. Close "Registry Editor"
6. Click Start and then Search" and then For Files or Folders ...
7. Type "LMMIB20.DLL" again and set Look in to be "Local Harddrives "
8. Delete any relevant entries.
There is back-ward and forward screening methods
For forward screening, you can do crosslinking and pulldown of candidate
proteins, and then send the products for mass spec
For back-ward screenings, you can use random KO library (usually with yeast)
to select the clones that are resistant to your ligand (with proper
screening assay), and then figure out what gene is required for the signal
pathway.
Either way it is not a small work
in these cases, both HA and IgG are background controls. Theoretically,
both of them should have no pulldown signals at all (= 0). If you divide
something by HA or IgG signal, any little variation will change your ratio
dramatically. So, neither of them is the right normalization.
A good normalization method is using 1% input as a control.
However, the best normalization is using some internal controls that are not
changed by your treatment. For example, your TF binds gene GAPDH and gene
X.
Yesh, for example you can use IP on endogenouse proteins
For the protein domain things, you can use the recombinant domain of A to
pulldown the endogenous proteins B
well, in this case, you can check with the other endogenous protein which is
not supposed to have interaction with A, and see if they also get pulldown.
try to check if there is difference in PI value between your protein and the
GST protein. If there is, then you can use ion exchange column to seperate
the GST protein and your GST-fusion protein after the glutathione-resin
pulldown.
Thank you much for your great contribution to my rookie question^^
I think non-related region negative control is convincing theoretically, if
not taking non- specific DNA precipitation into account: different DNA
region might have different tendencies of non specific precipitation (I also
learned that this issue can be simply avoid by using dynal beads)
A parable phenomenon is that when I was doing invitro protein interaction
assay such like GST pull-down and co-ip, the reviewer did not questi... 阅读全帖
Above paper its pp58
While we cannot dismiss the possibility that the split-luciferase
and the GST pulldown results are false-negatives, the interaction is
not supported by other evidence and is of lower confidence.
楼主 不会用排列组合吗?
萤火虫萤光标记N and C terminal a class
glutathione S transferase GST b class
HA-epitope tagged c class
then a must be used
positive control group:
(a,b,c)
(a,b)
(a,c)
negative control group:
(null, b,c)
(null, b)
(null, c)
Ps: if you are satisfied to this replied. BAOZI... 阅读全帖
