p*********g
发帖数: 9527 | 1 maybe you accidentally mutated a protein splicing recognition cite, so now
you are detecting the precursor instead of the mature one? |
|
W*******g
发帖数: 68 | 2 自己顶。
另外有没有人知道到哪里可以查到growth factor在水溶液里面的的degradation时间？
谢谢！
precursor |
|
s******y
发帖数: 28562 | 3 如果是特别常用的生长因子，那么可以买到的，
一般来讲，
Cell Signalling http://www.cellsignal.com/tools/cytokines/
Sigma, Santa Cruz 这几个公司是最可能有的
如果要找其他公司，不妨google 一下。
如果不是常用的因子，那么你可以找公司来帮你合成，
Genscript 这个公司合成的价格不贵，而且质量据说还可以。
precursor |
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o*****r
发帖数: 156 | 4 I have the latent form of TGF-beta.
precursor |
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j****x
发帖数: 1704 | 5 太懒啦...
From Wiki:
A splice site mutation is a genetic mutation that inserts or deletes a
number of nucleotides in the specific site at which splicing of an intron
takes place during the processing of precursor messenger RNA into mature
messenger RNA.The
abolishment of the splicing site results in one or more introns remaining in
mature mRNA and may lead to the production of aberrant proteins. |
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l*****g
发帖数: 43 | 6 translation和microRNA processing是同时进行的吗？有没有互相干扰？
请推荐文章，多谢 |
|
l*****g
发帖数: 43 | 7 俺自己回答一把。
至于translation和miRNAprocession有没有相互作用，相反的两个结论同时存在，都有
数据支持。
欢迎大家指教。 |
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s******y
发帖数: 28562 | 8 上来问一个学术问题，我们在用质谱来测生物样品里面的多肽组，
我们实验时的具体做法就是特异性的把多肽和蛋白分开，然后把多肽
送去用质谱测序
但是现在拿到raw data 之后不知道该blast against 什么数据库。
假如只是blast against 那种普通的蛋白数据库的话，只能告诉我们
那些多肽是来自于什么什么蛋白的（就是precursor)，但是我们想要的是
别人鉴别过了的多肽库，比方说 EGF啊，insulin啊什么的。
请问有没有人知道？谢谢了！ |
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K******S
发帖数: 10109 | 9 Here's my understanding: peptidome is the result of different proteolytic
enzyme activities. For traditional ID, you need to specify what enzyme you
are using in the search algorithm. such as trypsin, then the search
algorithm will look for K/R ending peptides to match. For peptidome, you can
ask the algorithm to search the entire database without any enzyme
restriction (It's gonna be a long search). then you can get some ladders of
peptides, I think you might be able to get some information by ... 阅读全帖 |
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p*****m
发帖数: 7030 | 10 跳过吵架贴哈
懂行的前辈冒个泡？我粗粗看了点review，主要是in vitro用stem/precursor cell研究
adipogenesis的居多，signaling pathway也搞得蛮清楚的，想知道你们觉得这个话题里
面还有的玩么？还有什么问题没解决没？ |
|
w***a
发帖数: 4361 | 11 in vitro adipogenesis有个最大的limitation就是无法区分
depot-specific differences，实际上身体各处的fat，
比如omental和subcutaneous fat 的behavior区别狠大。对于这个的研究，
不管是in vitro的3T3 cell 还是precursor stem cell都搞不定。 |
|
p*****m
发帖数: 7030 | 12 谢谢哈 那这个看起来是animal model可以搞搞的？另外你觉得在animal model看adipo
cyte怎么被环境影响（细胞数量，位置 vs diet, stress, etc）有做头没？
另外我想做cell line/precursor cell还有个没法看的就是有什么remote cue能影响，
比如说有个secreted protein来自pancreas会影响adipogenesis，这个In vitro没法看
？这么想有道理没？ |
|
m*****u
发帖数: 15526 | 13 另外，7天分化时间是不够的。7天只能看precursor的分化情况。更接近干细胞的细胞
需要更长的时间。一般半固体培养基也是14-21天观察。 |
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n********k
发帖数: 2818 | 14 This is a smart, very focused and productive one I have heard from people
working with him...XD Wang's first student was even more impressive if I am not too old:) and several from HJ Song's lab also have impressive records, A and P from this board both have impressive records in pretty shoret time too...
BTW, to upbeat/Q a bit for those (including myself) who may feel depressed now)....Nothing particularly/unusually, Pretty much decent intelligence, strong motivations/working/person habits/... 阅读全帖 |
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s*******s
发帖数: 623 | 15 谁叫人家是名人呢～
http://www.ncbi.nlm.nih.gov/pubmed/22123987
Proc Natl Acad Sci U S A. 2011 Nov 28. [Epub ahead of print]
Synthesis of three advanced biofuels from ionic liquid-pretreated
switchgrass using engineered Escherichia coli.
Bokinsky G, Peralta-Yahya PP, George A, Holmes BM, Steen EJ, Dietrich J,
Soon Lee T, Tullman-Ercek D, Voigt CA, Simmons BA, Keasling JD.
Source
Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608.
Abstract
One approach to reducing the costs of advanced bio... 阅读全帖 |
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t*d
发帖数: 1290 | 16 嚣张啊
Jinghui Zhang. et al. A novel retinoblastoma therapy from genomic and
epigenetic analyses. Nature. Published online 11 January 2012
Jinghui Zhang. et al. The genetic basis of early T-cell precursor acute
lymphoblastic leukaemia. Nature. Published online 11 January 2012
印象中芝加哥大学的 Lahn 作为通讯作者在同一期 Science 发过两篇。
|
|
w**********g
发帖数: 1985 | 17 http://pharmacy.whu.edu.cn/NewsDetail.asp?MaxSort=szdw&MaxUrl=t
目前以第一作者和通讯作者身份发表包括《Science》、《Annual Review of Genetics
》、《Metabolic Engineering》、《Chemistry & Biology》、《Methods in
Enzymology》等多篇高水平文章。
代表性论文：
1. Liu T, Khosla C. 2010. A Balancing Act for Taxol Precursor Pathways in E.
coli. Science. 330:44-45 （共通讯作者） |
|
i****g
发帖数: 3896 | 18 2006年-- 2007年
美国布朗大学化学系David E. Cane教授（美国科学促进会院士）实验室
博士生联合培养
晕，这个老板是AAAS fellow都要列出来，又不是多牛的头衔。
http://pharmacy.whu.edu.cn/NewsDetail.asp?MaxSort=szdw&MaxUrl=t
目前以第一作者和通讯作者身份发表包括《Science》、《Annual Review of Genetics
》、《Metabolic Engineering》、《Chemistry & Biology》、《Methods in
Enzymology》等多篇高水平文章。
代表性论文：
1. Liu T, Khosla C. 2010. A Balancing Act for Taxol Precursor Pathways in E.
coli. Science. 330:44-45 （共通讯作者） |
|
w**********g
发帖数: 1985 | 19 you are 悲愤ing. I do not悲愤 at all.
My point is that is this prospective a 论文?
代表性论文：
1. Liu T, Khosla C. 2010. A Balancing Act for Taxol Precursor Pathways in E.
coli. Science. 330:44-45 （共通讯作者） |
|
p*****m
发帖数: 7030 | 20 quote:
Science 1 October 2010:
Vol. 330 no. 6000 pp. 44-45
DOI: 10.1126/science.1195014
******PERSPECTIVE*********
CHEMISTRY
A Balancing Act for Taxol Precursor Pathways in E. coli
synthase |
|
c*******d
发帖数: 192 | 21 The Shapiro Laboratory at Columbia University in New York City, working
together with the NIH Vaccine Research Center (VRC), in Bethesda, MD, seeks
a postdoctoral or staff scientist-level researcher in computational biology
and bioinformatics. Work will involve analyses of high-throughput 454
antibody sequences from HIV infected patients. The goal is to understand
the maturation of these antibodies from their genomically-encoded precursors
in order to inform rational vaccine development. (See... 阅读全帖 |
|
K**4
发帖数: 1015 | 22 这个教授是名人啊
seeks
biology
precursors |
|
n***w
发帖数: 2405 | 23 You can also sort adipocyte precursor cells using FACS and the do some
functional assays. And IHC may give you informative results regarding APCs. |
|
g*********5
发帖数: 2533 | 24 I bought pCMV3Flag vector from sigma,
and now I quit, don't use this plasmid again.
My field is mitochondria,
and this plasmid produce a lot precursor protein... |
|
m******5
发帖数: 1383 | 25 直接表达后可用的，非precursor
google半日后只找到human版本 |
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l**********1
发帖数: 5204 | 26 LZ pls try go to
//www.sanger.ac.uk/genetics/CGP/cosmic/
or Cell article:
by
Nik-Zainal S et al. and Stratton MR (2012)
Mutational processes molding the genomes of 21 breast cancers.
Cell. 149: 979-93.
Abstract
All cancers carry somatic mutations. The patterns of mutation in cancer
genomes reflect the DNA damage and repair processes to which cancer cells
and their precursors have been exposed. To explore these mechanisms further,
we generated catalogs of somatic mutation from 21 breast cancer... 阅读全帖 |
|
j*b
发帖数: 341 | 27 这个PKM2和肿瘤代谢的关系已经知道很久了，这两篇还不是原创贴。
cantley还可以发nature，能不能解释一下？
Anticancer Res. 2003 Mar-Apr;23(2A):1155-8.
Tumor marker pyruvate kinase type tumor M2 in patients suffering from
diabetic nephropathy.
Oremek GM, Rutner F, Sapoutzis N, Sauer-Eppel H.
Source
Central Laboratory, Hospital of the Johann Wolfgang Goethe-University,
Frankfurt/Main, Germany.
Abstract
INTRODUCTION:
Compared to normal tissues all tumors investigated so far and especially
those with a metastatic potential overexpress an isoenzy... 阅读全帖 |
|
j*b
发帖数: 341 | 28 这个PKM2和肿瘤代谢的关系已经知道很久了，这两篇还不是原创贴。
cantley还可以发nature，能不能解释一下？
Anticancer Res. 2003 Mar-Apr;23(2A):1155-8.
Tumor marker pyruvate kinase type tumor M2 in patients suffering from
diabetic nephropathy.
Oremek GM, Rutner F, Sapoutzis N, Sauer-Eppel H.
Source
Central Laboratory, Hospital of the Johann Wolfgang Goethe-University,
Frankfurt/Main, Germany.
Abstract
INTRODUCTION:
Compared to normal tissues all tumors investigated so far and especially
those with a metastatic potential overexpress an isoenzy... 阅读全帖 |
|
J****n
发帖数: 156 | 29 Harvard E-journals竟然没有，有达人能帮我找找这篇文章吗？万分感谢。
J Biotechnol. 2012 Aug 31;160(3-4):146-50. doi: 10.1016/j.jbiotec.2012.02.
015. Epub 2012 Mar 21.
A simplified method for constructing artificial microRNAs based on the osa-
MIR528 precursor. |
|
s*****g
发帖数: 7857 | 30 是Glucosamine吗?常见的关节保健品.
常是三种合一Glucosamine + Chondroitin + MSM
Glucosamine (C6H13NO5) is an amino sugar and a prominent precursor in the
biochemical synthesis of glycosylated proteins and lipids. Glucosamine is
part of the structure of the polysaccharides chitosan and chitin, which
compose the exoskeletons of crustaceans and other arthropods, cell walls in
fungi and many higher organisms. Glucosamine is one of the most abundant
monosaccharides.[1] It is produced commercially by the hydrolysis of
... 阅读全帖 |
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A*******s
发帖数: 9638 | 31 有人认为是浪费时间和精力。
AAN: Stem Cells Show Promise in Mouse Model of ALS
THURSDAY, Jan. 10 (HealthDay News) -- Implantation of a neural stem cell (
NCS) fraction of induced pluripotent stem cells (iPSCs) shows promise in a
mouse model of amyotrophic lateral sclerosis (ALS), according to a study
released in advance of its presentation at the annual meeting of the
American Academy of Neurology, which will be held from March 16 to 23 in San
Diego.
Stefania Corti, M.D., Ph.D., from the University of Milan, ... 阅读全帖 |
|
G***G
发帖数: 16778 | 32 how to convert .mzxml to .dta?
how to set the following parameters:
1)precursor charge state range
2) minimum MH+ mass and maximum MH+ mass |
|
q******g
发帖数: 3858 | 33 Andraka cultured MIA PaCa cells, from a commercial pancreatic carcinoma cell
line, which overexpress mesothelin, a biomarker for pancreatic cancer. The
mesothelin was isolated, concentrated and quantified with ELISA.[6] After
optimization with the Western Blot assay, the human mesothelin-specific
antibodies were mixed with single walled carbon nanotubes and used to coat
strips of ordinary filter paper. This made the paper conductive. The optimal
layering was determined using a scanning electron ... 阅读全帖 |
|
a****d
发帖数: 1919 | 34 The radial glia cells divide asymmetrically and produce precursor/immature
neurons, which migrate along the radial process up towards the cortical
plate.
Neurons were born first,the glia cells were produced later during cortical
development. Both are originated from the radial glia cells. Recently there
are findings suggesting that glia cells divide locally at the upper cortex
to increase the glia population.
Hopefully this is helpful. |
|
l********n
发帖数: 260 | 35 Absence of A673T amyloid-β precursor protein variant in Alzheimer's disease
and other neurological diseases
please send to j***********[email protected]
Thx a lot |
|
s******9
发帖数: 283 | 36 很久以前从yeast转到E.coli合成，现在又是在yeast。有牛人评论一下为什么还能发
Nature？就靠提高了产量和Keasling的名头？这么多年，投入多少钱和人啊。。。
中间体artemisinic acid看起来不很复杂，化学方法真的很难？
High-level semi-synthetic production of the potent antimalarial artemisinin.
http://www.nature.com/nature/journal/v496/n7446/full/nature1205
In 2010 there were more than 200 million cases of malaria, and at
least 655,000 deaths1. The World Health Organization has recommended
artemisinin-based combination therapies (ACTs) for the treatment of
uncomplicated malaria c... 阅读全帖 |
|
D****g
发帖数: 275 | 37
I am not in cancer field, I can not give exact answer about whether there is
liver cancer originated from blood or not, but I guess the answer should be
YES. Because both spleen and liver get cancer in your mice and both tissues
have cells originated from bone morrow (macrophages for both tissues and
erythroblast precursor for spleen), there is high chance that the cancer is
related with bone marrow, it will be very helpful if you can check the cell
population in the bone marrow, blood and sple... 阅读全帖 |
|
r******g
发帖数: 600 | 38 下午在实验室，木有中文输入~ 不好意思
monocyte是macrophage的precursor细胞~ 在circulation里面应该是木有什么
antipathogenic function的！
对于sepesis这种情况，我也不是特别清楚具体自身免疫系统有多强大的能力来控制
sepesis. 理论上来说，你可以理解脾脏是一个大血泡，circulation的血液都会循环经
过spleen~ spleen是人体最大的淋巴器官，因为，他可以filter血液的细菌啊什么的！
当血液不干净的时候，脾脏的pulp有一层macrophage lining，那些macrophage可以把
细菌吞了。
这些只是理论，实践中，一旦病人有sepesis，自身免疫系统木有什么能力来对付! 如
果医疗不介入，患者多半是个死！白求恩不就是得败血症死的么...
绝大多数感染是不侵犯循环系统的，只有infection progression到很严重的时候才会
出现bacteremia. |
|
a**********e
发帖数: 418 | 39 1）无法证明生命的来源 is a precursor for Evolution---also called Evil
Illusion.
That question cannot be answered by biologist. Chemistry and physics might
help.
棲， |
|
d****h
发帖数: 46 | 40 For rat neuron
GABRA1: (Neuron. 1989 Dec;3(6):745-53.A novel alpha subunit in rat brain
GABAA receptors.Khrestchatisky M, MacLennan AJ, Chiang MY, Xu WT, Jackson MB
, Brecha N, Sternini C, Olsen RW, Tobin AJ.)
GABBR2:similar to GABRA1, highly neuron-specific
MAP2:(A Harada, J Teng, Y Takei, K Oguchi, N Hirokawa
MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and
proper PKA signal transduction J Cell Biol, 158 (2002), pp. 541–549)
Tuj1: (J Neurobiol. 1995 May;27(1):26-43.Div... 阅读全帖 |
|
j****x
发帖数: 1704 | 41 有转染效率的control吗？效率不够的话看不到表型很正常，miRNA转染虽说并不tricky
，但有时候也会不稳定。
用质粒表达miRNA不稀奇。你直接转染的其实是miRNA mimics,拿载体表达的是miRNA
precursor. |
|
f******g
发帖数: 1003 | 42 老的T细胞如果不被新生取代的话，容易生成tumor。
Cell competition is a tumour suppressor mechanism in the thymus
Cell competition is an emerging principle underlying selection for cellular
fitness during development and disease. Competition may be relevant for
cancer, but an experimental link between defects in competition and
tumorigenesis is elusive. In the thymus, T lymphocytes develop from
precursors that are constantly replaced by bone-marrow-derived progenitors.
Here we show that in mice this turnover is regula... 阅读全帖 |
|
s*****5
发帖数: 52 | 43 问版上做质谱的行家，有重复过Josh coon几年前发表的那个ETD/CID decision tree的
方法吗?就是用ETD碎裂高电荷低M/Z的precursor,其他的用CID. 我最近在Orbitrap
Fusion上试图重复，发现ETD使CID的scan数也减少了很多，最后还不如只用CID。只用
ETD得到的蛋白数比CID少非常多，觉得可能是反应时间设的过长,但是设的短谱图质量
看起来很低。顺便求问如何优化ETD的参数及注意事项等，谢谢！ |
|
r*****m
发帖数: 3619 | 44 CID 的缺点一个是看不到小于 precursor ion MW 三分之一的 fragment ion, 另一个
是容易打掉蛋白的修饰基团，对修饰基团的定位带来困难。
ETD 可以克服above两个问题，但是对sample的纯度和用量，都比 CID 高。
Fusion 真正高级的地方，是在做HCD时可以连续把 MSn 做下去。 |
|
r*****m
发帖数: 3619 | 45 定量还是用 triple quadrupole 做真正的 precursor ion scanning，拿标准品建立
multiple reaction monitoring
Psmart,
Cycle |
|
k****o
发帖数: 728 | 46 什么叫“对sample的纯度和用量，都比 CID 高”？
做MS2之前都是要isolate precursor ions。 |
|
r*****m
发帖数: 3619 | 47 ETD 对 precursor ion 的 ion abundance 要求高 |
|
k****o
发帖数: 728 | 48 看你怎么看了。ETD确实是charge assumption的技术。如果想把precursor全部与ETD
reagent anion反应完，则需要比理论optimized reaction更多的时间，而这也同时带
来使First-generation fragment ions继续与reagent ion react产生internal
fragments和更多的small N- and C-term fragment ions。对于不大的peptide（比如
3000 Da以下的），延长reaction time来提高fragment intensity没什么大问题，因为
靠+1, +2 fragments你就能ID或characterize peptide了。 而对于大的peptide，这
样做会产生internal fragmentation（这些internal fragment你是看不到的，他们“
沉淀”成为MS2 background），会让比较small的terminal fragment ions增多（
signal增强），你只能得到比较好的terminal seq... 阅读全帖 |
|
s*****5
发帖数: 52 | 49 data dependent scan的话， 会选取一些最abundant的precursor做MS2.如果您说的是
targeted scan，的确对样品中目标肽的要求高。 |
|
k****o
发帖数: 728 | 50 忘记问了，你precursor isolation用的是quadrupole isolation吧。别用IT
isolation。OF用quadrupole isolation是Thermo建议的，原因这里就不细说了。跟过
去变化很大。 |
|
