G*******g
发帖数: 556 | 1 no, the radiation is nucleus level, not atom/ion level... |
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l*******1
发帖数: 128 | 2 大脑知道是几点钟，因为大脑有一个生物钟，最高生物钟在视交叉上核（
suprachiasmatic nucleus, SCN)。其它组织，比如肝脏，也有生物钟，称为外周生物
钟(peripheral clocks），受SCN生物钟的调控。肝脏生物钟还受饮食习惯的调控－－
所以吃饭要定时。
SCN生物钟的时间（可以想象成一个圆周循环的相位）受到几个主要的环境因子的调控：
1。光－－冬天抑郁症采取光疗法的依据。
2。温度。
3。饮食规律。
所以说到楼主MM的生物钟，我觉得只要她每天在白天同一个时刻睡觉，关上门窗，晚上
吃三餐，她的生物钟不会有大问题。她的体温最低点可能就在白天而不是晚上3点了。 |
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a******e
发帖数: 17 | 3 以下是我一个哈佛物理博士朋友的回答。但愿能给你一点安慰。。。
Our family is planning a east coast vacation (for real) to outrun the
nuclear cloud drifting our way... I am kidding, but you may find the
following information entertaining, and potentially educational.
We need to put any risk in perspective. The main stream media is playing a
constructive roles here to report the risk as justified by the information
available and by the possible worst-case scenarios. Risk cannot be
discussed without quantification, otherwise i... 阅读全帖 |
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S*********g
发帖数: 24893 | 4 【 以下文字转载自 StephenKing 俱乐部 】
发信人: StephenKing (金博士), 信区: StephenKing
标 题: 不系安全带，John Nash今天车祸死了
发信站: BBS 未名空间站 (Sun May 24 11:17:59 2015, 美东)
John Forbes Nash, Jr.
Born June 13, 1928
Bluefield, West Virginia, U.S.
Died May 23, 2015 (aged 86) [1]
Residence United States
Nationality American
Fields
Mathematics
Economics
Institutions
Massachusetts Institute of Technology
Princeton University
Alma mater
Princeton University
Carnegie Institute of Te... 阅读全帖 |
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b*s
发帖数: 82482 | 5 http://skyserver.sdss.org/dr1/en/proj/advanced/galaxies/spectra
When you look at the spectrum of a galaxy, you are really looking at the
combination of spectra from the millions of stars in the galaxy. So studying
the features of a galaxy spectrum tells you about the types of stars the
galaxy contains, and the relative abundances of each type of star.
Galaxy spectra also clearly show you whether a galaxy contains star-forming
regions called HII regions. HII is a spectral emission line that corre... 阅读全帖 |
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l*********1
发帖数: 2971 | 7 Egg Quality
Egg quality is often blamed as the cause for IVF failure. Improving egg
quality gives you a better outcome from subsequent IVF cycles and also
contributes to having a healthy pregnancy and baby.
One of the reasons that the focus is more on egg quality than sperm quality
is that the egg contributes all the mitochondria for the embryo.
Mitochondria are tiny organelles in every cell of your body where energy is
produced. All the mitochondria in the embryo come from the egg – none come
f... 阅读全帖 |
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S**C
发帖数: 2964 | 8 http://www.nytimes.com/2014/02/16/opinion/sunday/is-the-univers
IN Mikhail Bulgakov's novel “The Master and Margarita,” the protagonist, a
writer, burns a manuscript in a moment of despair, only to find out later
from the Devil that “manuscripts don’t burn.” While you might appreciate
this romantic sentiment, there is of course no reason to think that it is
true. Nikolai Gogol apparently burned the second volume of “Dead Souls,”
and it has been lost forever. Likewise, if Bulgakov had burned his
... 阅读全帖 |
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n*********0
发帖数: 925 | 9 http://www.glorypress.com/devotional/FaithAndLifeOneYearBook.asp?bid=3
上帝的乘法表
经文:「至小的族要加增千倍；微弱的国必成为强盛，我──耶和华要按定期速成这事
。」（以赛亚书六十︰22）
上帝的工作刚开始时，常是微不足道的，但这也没什么不好，软弱使我们接近
上帝，困境可操练我的信心，使上帝的名可大得荣耀。上帝应许我们要繁荣倍增，就像
芥菜种子在所有植物当中是最小的，但却可长成像一颗树那样大，让飞鸟来栖息。我们
开始可能只是「一」，而且是小小的「一」，但它可变成千，这是上帝奇妙的乘法表。
上帝不是常对祂的仆人说︰「我要使你结实百倍、千倍！」信靠上帝，祂会让你得人如
得鱼，而且人数渐多，因为只要奉耶稣的名聚在一起，上帝必要在我们当中。
「小小的一个」，这在以量取胜的世人眼中来看，是多么地令人泄气。然而行
远必自迩，登高必自卑，万丈高楼也是平地起。夜里只要第一颗星开始亮，转瞬间，繁
星就将陆续出现，布满夜空。
我们也不要臆测这样的应许，只不过是个远景，并非指日可待的美梦。... 阅读全帖 |
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J*****3
发帖数: 4298 | 11 笑死我了，还来玩黑白颠倒的吗？
我给你全文吧，这么撒谎的话，是耶稣教的吗？撒谎神不喜悦
这篇文章是基督教手册用来歪曲人类进化不成立的，是JAMES给我了文章的一个LINK，但是文章看不全，我根据作者名字找到了全文，也读了，发现，文章根本没有说进化不成立，他们断章取义，黑白颠倒地撒谎。我BS这种恶心的行为。
“5、大概在97年，一个著名的非基督徒考古学家，在非洲发现的所谓人类化石，把一
直以来公认的人类进化顺序，猿人——直立人——能人——智人，给彻底推翻了。目前
人类进化链条重新归零，变为空白。”
原文
http://news.nationalgeographic.com/news/2010/05/100506-science-
翻译：
“Even if the new result doesn't quite settle the debate about whether
Neandertals mixed with modern humans, it does underscore how different they
were from our own lineage. And t... 阅读全帖 |
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s*********a
发帖数: 801 | 12 看到后面的那个链接，终于明白你在说什么了。
我还以为你很专业呢，你怎么也不研究一下就人云亦云呀。
- 声明一下，不是我译的。不过，就算“心脏”错用在“aortic arch”，对全
文无伤大雅。
vagus nerve 到 aortic arch 那里分叉，分出一条recurrent laryngeal nerve，正好
在主气管的下端，由下往上，控制颈部肌肉。有什么问题吗？
你贴的那个文章一开头就错了，什么绕过心脏，明明是在主动脉那里分出的支神经，你
最好量量看有多长，再决定是不是绕了弯路，可别把vagas nerve也一块儿量进去了。
我估计你贴的文章里就把vagas nerve也当recurrent laryngeal nerve算了。
－ 有问题，recurrent laryngeal nerve 的 nucleus (nuclei) 在哪里？
superior laryngeal nerve 在哪里从 vagus nerve 分叉处来的？同是通向 larynx,
为什么一条直接连接，另外一条拐了一个弯？recurrent laryngeal nerve 是控制“颈
... 阅读全帖 |
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P******l
发帖数: 1648 | 14 来自主题: TrustInJesus版 - 人类的肤色 WHAT COLOR WAS ADAM?
In western countries, nearly every imaginative painting of Adam and Eve
depict two adult Caucasians with fair skin and blue eyes. These images, even
used as Bible illustrations, tend to shape the reader's mental image of the
first man and woman. The Sunday-school origin of the dark races is often
that they were descendants of Adam and Eve who had migrated to a hot climate
where the suntan eventually became an inherited characteristic. These
images and explanations discredit ... 阅读全帖 |
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g******d
发帖数: 1774 | 16 it's really puzzling, to be honest. i don't think low turn-around number is
the problem, rather, we should encourage all of us already here to post
actively, to become a nucleus that other fellow njuers will like to be
around.
please share with us your ideas, recommendations. it's up to each one of us. |
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b*******g
发帖数: 1309 | 17 become a nucleus ????
晶体长多了吧
呵呵
is
us. |
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g******d
发帖数: 1774 | 18 from webster dictionary
4 : a basic or essential part : CORE
team> |
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w*********s
发帖数: 618 | 19 神经科学顶级期刊Neuron刊登南京大学王建军教授、朱景宁副教授课题组研究成果
[发布时间：2011-03-07] [字体大小： 小 中 大]
神经科学顶级期刊Neuron（神经元，系Cell Press杂志社出版的Cell杂志姐妹
期刊）最近刊登了南大生命科学学院和生物医药技术国家重点实验室王建军教授、朱景
宁副教授课题组的研究论文“A role for orexin in central vestibular motor
control”（Neuron, 2011, 69:793-804）。
Orexin是一种新近发现的小分子神经肽，因其强烈的促食欲作用而得名。已经
发现，起源于下丘脑外侧区/穹窿周区的中枢orexin能神经系统在机体能量代谢、药物
成瘾、奖赏和情绪反应等许多非躯体性活动中发挥重要功能。有趣的是，orexin缺乏可
致人和动物发生嗜睡-猝倒症（narcolepsy- cataplexy），一种表现为白天无法用意志
控制的过多睡眠发作以及毫无预警的突然昏睡或猝倒。该病遍发于世界各地，且不分年
龄种族。猝倒发作时患者虽意识清醒，但却因躯体... 阅读全帖 |
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h******i
发帖数: 13 | 20 电子科技大学能源科学与工程学院
诚邀海内外高层次人才加盟
UESTC SESE Warmly Welcomes Young Professionals
一、学校概况
I. About UESTC
电子科技大学是国家首批“211工程”和“985工程”重点建设大学，是中国39所“985
工程”入选高校中唯一一所电子信息类高校，也是全国第一所在“电子科学与技术”和
“信息与通信工程”两个一级学科中所有二级学科均为国家重点学科的高校。
University of Electronic Science and Technology of China (UESTC for short)
is one of the first 211 Project and 985 Project universities, and also, the
only electronic information–oriented university. It is the first university
in China with all the sub-disciplines of the two discipli... 阅读全帖 |
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h******i
发帖数: 13 | 21 电子科技大学能源科学与工程学院
诚邀海内外高层次人才加盟
UESTC SESE Warmly Welcomes Young Professionals
一、学校概况
I. About UESTC
电子科技大学是国家首批“211工程”和“985工程”重点建设大学，是中国39所“985
工程”入选高校中唯一一所电子信息类高校，也是全国第一所在“电子科学与技术”和
“信息与通信工程”两个一级学科中所有二级学科均为国家重点学科的高校。
University of Electronic Science and Technology of China (UESTC for short)
is one of the first 211 Project and 985 Project universities, and also, the
only electronic information–oriented university. It is the first university
in China with all the sub-disciplines of the two discipli... 阅读全帖 |
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q**w
发帖数: 782 | 22 今年生物似乎不错
http://www.nature.com/nsmb/journal/vaop/ncurrent/full/nsmb.2959
NATURE STRUCTURAL & MOLECULAR BIOLOGY | ARTICLE
Exon-intron circular RNAs regulate transcription in the nucleus
Zhaoyong Li, Chuan Huang, Chun Bao, Liang Chen, Mei Lin,
Xiaolin Wang, Guolin Zhong, Bin Yu, Wanchen Hu, Limin Dai,
Pengfei Zhu, Zhaoxia Chang, Qingfa Wu, Yi Zhao, Ya Jia, Ping Xu,
Huijie Liu & Ge Shan |
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T********l
发帖数: 1670 | 23 原理上是对的，不过需要强调的是 1. 南极很干燥，而且温差又非常至少， 好像记得只
有5度，这一点点的温度的落差很难让很干的空气变成过饱和而形成condensation。 2.
形成condensation另外一个条件是：seeds or nucleus，因为南极太干净了,而缺少象
陆地形成condensation 足够的seeds：越有seeds，越容易形成condensation。当然
seed的大小会控制condensation的大小，所以有时会形成液滴，有时会是水汽， 也就
这个道理。
呵！ |
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g******y
发帖数: 21 | 24 Below这些软件在使用CPANEL为控制面板的
hosting上面，点击几下就安装完了，都不用下载调试和安装。
我现在用的gohomehost.com 就是这样的。
具体cpanel可以一键安装的主流系统见下面：
* Blogs: b2evolution, Nucleus, WordPress
* Content Management: Drupal, Geeklog, Joomla, Mambo, PHP-Nuke, phpWCMS,
phpWebSite, Siteframe, TYPO3, Xoops, Zikula
* Customer Relationship: Crafty Syntax Live Help, Help Center Live,
osTicket, PerlDesk, PHP Support Tickets, Support Logic Helpdesk, Support
Services Manager
* Discussion Boards: phpBB, SMF
* E-Commerce: CubeCart, |
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s****n
发帖数: 786 | 25 当然可以，buildroot+uclibc+busybox轻松到300k，按lz的要求减100k太容易了。
Vxworks, Nucleus, ThreadX,QNX，这些kernel最小可以只有6-8k，Linus应该后悔没有
实现微内核。
编译后几百 K的C源代码怎么也有两三万行吧，你随便写写也忒厉害了。 |
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d****i
发帖数: 4809 | 26 C#是有一些工作的，毕竟Windows下它是主流，但是随着微软的萎缩，桌面需求的下降
，Windows会逐渐萎缩，现在的客户端平台除了Windows基本都是Unix的体系出来的：
Android: Linux
iOS: Unix-like
BlackBerry OS: QNX (Unix-like)
Tizen: Linux
Firefox OS: Linux
WebOS: Linux
Chrome OS: Linux
这还不包括很多运行在嵌入式系统上的实时操作系统，它们中很多也是Unix-like，象
VxWorks, Integrity, Nucleus, ThreadX等等。
然后服务器端，除了Windows以外也都是Unix血统
Linux (Ubuntu, Fedora, Red Hat Linux, SUSE, CentOS, Debian)
IBM AIX (Unix)
HP/UX (Unix)
Sun Solaris (Unix)
DEC/Compaq Tru64 (Unix)
FreeBSD (Unix)
OpenBSD (Unix)
Mac OS (Unix-lik... 阅读全帖 |
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c******e
发帖数: 545 | 27 也不能这么绝对，解决方案好的话普及也有可能。联发科好像是用Nucleus RTOS的吧。
解决方案齐全，通吃山寨机 |
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d****i
发帖数: 4809 | 28 你搞反了，数百种RTOS都是C写的，现在RTOS虽然受到embedded Linux的一定的冲击，
但在各种工业领域还是大量使用的，比如VxWorks, QNX, Integrity，RTX, uC/OS，
Nucleus, ThreadX等等，这些RTOS实际上就是相当于嵌入式系统上的UNIX。 |
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d****i
发帖数: 4809 | 29 你这个显然不对，各种工业上用的实时操作系统，既不是UNIX, 也不是Linux，更不是
Windows, 而是专用的实时操作系统，RTOS有几百种，常见的几种有比如
VxWorks
QNX
Greenhills Integrity
Nucleus
ThreadX
RTX
当然实际上他们的设计都深受UNIX设计思想和架构的影响，就是把普通台式机和服务器
的操作系统迷你化，实时化，和专用化后的一个个专用的操作系统。至于你说的多数都
是windows的，都是给人用的控制台程序，带有UI界面的，和实时操作系统完全是两码
事。 |
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d****i
发帖数: 4809 | 30 Linux, UNIX, Windows这些通用操作系统都不是real time的，真正的real time的操作
系统都是专门用于embedded上的像VxWorks, QNX，Integrity, Nucleus, RTX等等。
MSN |
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d*****u
发帖数: 17243 | 31 那其实我们说的是一回事
只是我不倾向于认为-r是“韵尾”，实际上它常常是音节的核心成分(nucleus)
所以我把-r~看成是鼻化元音儿化的结果，而-r则是普通元音儿化的结果
这里所说的“鼻化”也不等同于an,ang中出现的那个音位变体[a~]
而是phonological rule在作用时的一个中间状态
我现在手里有端木三在MIT的博士论文，就是关于汉语音系的
我发现他没有从非线性音系学的角度来解释为何-n,-ng儿化的结果不一样
只是提出一个语音学上的说法：北京话的-ng可能是nasal glide，而不是nasal stop
这还是有一定道理，我注意到北京话的ang收尾时没有成都话“紧”
而且不像成都话ng可以作声母
如果此说成立的话，说明ang韵母在北京话里其实就是一个鼻化（双）元音而已
它儿化以后仍然保持[+nasal]特性
而an韵母存在一个delinking，丧失了[+nasal]特性 |
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s******o
发帖数: 298 | 32 By Brian Berger
Space News Staff Writer
posted: 12:23 pm ET
28 June 2002
WASHINGTON — The discovery of dust on the solar array panels of the Comet
Nucleus Tour spacecraft Thursday prompted officials to halt preparations for
the planned July 1 launch of the comet-bound NASA vehicle.
The launch has been postponed to no earlier than July 3 while the mission
team investigates what is on the solar array and how it got there.
The spacecraft, called CONTOUR for short, is an eight-sided spacecraft built |
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s******o
发帖数: 298 | 33 By Jonathan McDowell
July 3, 2002 | At 2:47 a.m. EDT this morning, the Applied Physics Laboratory's
Contour comet probe launched from Cape Canaveral, Florida, aboard a Delta 7425
rocket en route to its elliptical, 109,000-kilometer apogee orbit around
Earth. Contour (a contraction for Comet Nucleus Tour) will spend until August
15th in this parking orbit. On that date a solid-fuel rocket will put Contour
in its intended solar orbit. This new path will range between the orbits of
Venus and Mars, |
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s******o
发帖数: 298 | 34 July 24, 2002 | It seems another comet is dissolving into nothingness, right
in front of astronomers' eyes. On July 13th, the NASA Jet Propulsion
Laboratory's Near Earth Asteroid Tracking program (NEAT) reported finding a
peculiar object with a faint coma. Calculations at the Minor Planet Center
soon revealed that the small body had the same motion as the periodic comet
57P/du Toit-Neujmin-Delporte but was separated from its nucleus by some 0.2
degree.
Alerted by those observations, a team of as |
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s*****t
发帖数: 1994 | 35 Spiral Galaxy NGC 2997 from VLT
Explanation: NGC 2997 is a grand design spiral galaxy. Its small nucleus and sprawling spiral
arms give it a type Sc designation. NGC 2997, pictured above, is speeding away from us at
about 1100 kilometers per second, which would place it at about 55 million light years distant,
given current estimates of the expansion rate of our universe. NGC 2997 is thought to have a
mass of about 100 billion times that of our Sun, but is probably less massiv |
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s*****t
发帖数: 1994 | 36 Spiral Galaxy NGC 300
Credit: M. Schirmer (IAEF, Bonn), W. Gieren (Univ. de Concepcisn, Chile), et al., ESO
Explanation: NGC 300 is so interesting because it is so normal. An Sc-type spiral galaxy in the
nearby Sculptor group of galaxies, NGC 300 shows typical flowing blue spiral arms, an expected
compact nucleus, and the requisite amount of stars, star clusters, and nebulae. Therefore,
studying NGC 300 should indicate how, exactly, a normal spiral galaxy works. Toward this goal,
NGC 300 and t |
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s*****t
发帖数: 1994 | 37 Comet Neat Passes an Erupting Sun
Credit: SOHO Consortium, LASCO, ESA, NASA
Explanation: As Comet NEAT flared last week, the Sun roared. Just as the comet swooped
inside the orbit of Mercury and developed a long and flowing tail of gas and dust, the Sun
emitted a huge Coronal Mass Ejection (CME). Neither the fortuitous hot ball of solar gas nor
the intense glare of sunlight appeared to disrupt the comet's nucleus. The action was too close
to the Sun to be easily visible by humans, |
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a*****t
发帖数: 81 | 38 这篇文章我读过, 发表在The Journal of Neuroscience, September, 1999.
Reconstruction of Natural Scenes from Ensemble Responses in the
Lateral Geniculate Nucleus. Garrett B. Stanley, Fei F. Li, and
Yang Dan. 注意到老板 Yang Dan 是个很漂亮的年轻中国姑娘, (网址:
http://mcb.berkeley.edu/faculty/NEU/dany.html) 想想人家30岁就拿到
UCBerkeley 的 assistant professor了, 有时觉得自己选择做 neuroscience
是不是一个错误.
这篇来自sina.com的文章有几个小错误, 首先177个外膝体细胞不是同时记录到的,
而是采用相同的视觉刺激, 分11次收集到的, 但是它们的receptive field各不
相同. 其次这还只是对于视觉重建(reconstruction of spatiotemporal visua |
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a*****t
发帖数: 81 | 39 这篇文章我读过, 发表在The Journal of Neuroscience, September, 1999.
Reconstruction of Natural Scenes from Ensemble Responses in the
Lateral Geniculate Nucleus. Garrett B. Stanley, Fei F. Li, and
Yang Dan. 注意到老板 Yang Dan 是个很漂亮的年轻中国姑娘, (网址:
http://mcb.berkeley.edu/faculty/NEU/dany.html) 想想人家30岁就拿到
UCBerkeley 的 assistant professor了, 有时觉得自己选择做 neuroscience
是不是一个错误.
这篇来自sina.com的文章有几个小错误, 首先177个外膝体细胞不是同时记录到的,
而是采用相同的视觉刺激, 分11次收集到的, 但是它们的receptive field各不
相同. 其次这还只是对于视觉重建(reconstruction of spatiotemporal visua... 阅读全帖 |
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r***o
发帖数: 162 | 40 you can not simply say whether the result of an experiment right or
not,
I have read papers of both sides for the origin of that lovely
protein virus(virus?), I even have asked my professor, he dare not
judge
either, by the way, he is a really excellent virologist, and editor of
RNA magazine, :)
one thing, I think we need to add to the answer of the question is
that
some DNA viruses can enter nucleus, with histones, forming a structure
just like mini-chromosome, for transcription and replication |
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M****e
发帖数: 70 | 41 this is a good paper that tries to dissect the regulatory
mechanism of strict control on DNA replication during cell
cycle. to ensure only one round of DNA replication at each
S phase, yeast S. cerevisiae exploits cdk2 kinase to regulate
functionally reduntant pathways: degradation of Cdc6, export
of MCM from the nucleus, and phosphorylation of ORC. however,
the mechanism in metazoan is unclear while the cellular
targets of cdk2 have not been completely understood yet.
the author (only one besid |
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O******e
发帖数: 4845 | 42 ☆─────────────────────────────────────☆
idiotic (confused) 于 (Fri Jan 19 21:48:19 2007) 提到:
For pioneering biochemical studies on apoptosis, which have elucidated a
molecular pathway leading into
and out of the mitochondrion and to the nucleus.
Former winners include Brown and Goldstein, Alfred Gilman, Gunter Blobel,
Richard Axel, Stanley Prusiner,
John Kuriyan...
☆─────────────────────────────────────☆
forfun2060 (悠悠观天下) 于 (Sat Jan 20 00:34:17 2007) 提到:
值得骄傲!会有越来越多的华人在这个领域里作出惊人的成绩来的, |
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p*****n
发帖数: 981 | 43 ☆─────────────────────────────────────☆
else (where) 于 (Mon Jan 22 14:29:08 2007) 提到:
Overexpressed one protein in human cell line and found the granular staining
pattern, seems like intracelluar transport vesicles. Can anybody give some
hints about the cellular compartments? See attached pics (green is
overexpressed protein, blue is nucleus).
Thanks
☆─────────────────────────────────────☆
Biomacro (革命好青年) 于 (Mon Jan 22 14:38:25 2007) 提到:
像intrcellular vesicles.
不过有个疑问，你是用DAPI染的核吧？为啥核周 |
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p*****n
发帖数: 981 | 44 ☆─────────────────────────────────────☆
Oncogene (基因头) 于 (Tue Jan 30 00:25:07 2007) 提到:
☆─────────────────────────────────────☆
idiotic (confused) 于 (Fri Jan 19 21:48:19 2007) 提到:
For pioneering biochemical studies on apoptosis, which have elucidated a
molecular pathway leading into
and out of the mitochondrion and to the nucleus.
Former winners include Brown and Goldstein, Alfred Gilman, Gunter Blobel,
Richard Axel, Stanley Prusiner,
John Kuriyan...
☆────────────────────────────────── |
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a***e
发帖数: 1010 | 45 (1) if your protein localizes in cytoplasm, a gentle lysis will only release
your protein to the solution, but not chromatin DNA.
(2) if your protein accumulates in nucleus but not sticks to DNA, you can
try several rounds freeze-thaw in some buffer with detergent. Then spin down
. The chromatin DNA will stay in pellet.
(3) and you can always add a pre-clear step with beads only. |
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a***e
发帖数: 1010 | 46 your procedure has some problems. (1) the first NP-40 10 min is too long. (2
) the second 2x wash is not enough to remove residue cytoplasm.
think about this way, if you don't care about recovery efficiency:
for the first step, 0.5% NP40 for only 1 min, transfer supernatant to new
tube. this should be very clean cytoplasm.
for the second step, 0.5% NP40 for 10 min, wash 2x, save pellet this time.
this should be very clean nucleus.
I mean 1 min and 10 min are very extreme examples. |
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s******y
发帖数: 28562 | 47 Almost the same for staining nucleus.
But for staining small DNAs such as the the mitochondria plasmid, Hoechst is
better. |
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L****S
发帖数: 76 | 48 Dear all,
I got another problem for Image J. My boos asked me to score TUNEL staining
for apoptosis, that means I need to score almost 1000 DAPI (nucleus) to see
among them how many TUNEL positive cell according to her. That is a lot of
scoring under microscope.
I wonder whether I can take images and use Image J to score DAPI (I just
need to score TUNEL).
Is there anyone knows how to use Image J to score DAPI?
Thank you.
Lei |
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