p*****m
发帖数: 7030 | 1 前几天刚讨论过不少 俺在准备这周的journal club打算就讲这个了 仔细读了读还有不
少问题 大家帮忙哈
1 感觉文章行文逻辑有点奇怪 按说既然打算做mammal的Co2 sensing 首先要做的肯定是
看behaviorally动物怎么感知Co2 感知的浓度范围阿 对年龄性别温度湿度circadian r
hythm有没有要求 这些数据对他们做后来的电生理/imaging都是必需的 可是他们却把行
为放到fig4 把CAII+ neuron的molecular identity放到fig1 俺理解得对不对？
2 还是和1有关的。CA和CO2 sensing有关看起来在别的动物里做过不少了，不过CAII在
mouse里和CO2 sensing有关应该是第一个报道。那么当他们发现CA inhibitor能够影响
CO2 sensing behaviorally之后 如果想要知道这些CAII-expressing neuron的molecul
ar nature的话最简单的方法就是做个micorarray看看CAII+ cell里面还表达什么特殊的
东东。不过他们却很奇怪的从PDE |
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B******1
发帖数: 9094 | 2 They would schedule a hearing and ask the poor creature who caused its
misery. Animal protection groups would stage a protest and demand the
immediate release of the fuzzy ball and its safe return to its habitat. Due
to the enormous outcry from the media and the general public, the
institution would have no choice but to set the lab rat free at a live-
televised ceremony, attended by such notables as Presidents Barack Obama and Jintao
Hu. Once running wild in nature, the appreciative mammal wou |
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p*****m
发帖数: 7030 | 4 janelia farm只做两件事 发展imaging技术 研究neural circuitry。这两个目标号称是
是HHMI找一堆牛人关门研究的结果 觉得这两个方向代表了neuroscience的前途。
他们当然是有mammal的人了 做neural circuitry的 |
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p*****m
发帖数: 7030 | 5 欢迎总结啊 不过不能认同你对worm/fly的看法 RNAi在mammal里面in vivo的应用屈指
可数 效果也差 (in vivo)光从提供了一个简单高效的in vivo system这一个好处出发
fly/worm就
不是能被淘汰的 工具方面 mammalian系统抗体是多 可架不住fly/worm随便能弄个GFP
reporter 很多时候压根就不需要抗体
当然mammalian people对yeast/fly/worm有偏见 NIH也有很强的指向性 这都是事实 |
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p****y
发帖数: 23737 | 6 你有没有看电影The Matrix？里面很有名的一句话：
A virus. Human beings are a disease, a cancer of this planet.
“Every mammal on this planet instinctively develops a natural equilibrium
with the surrounding environment but you humans do not. You move to an area
and you multiply and multiply until every natural resource is consumed and
the only way you can survive is to spread to another area. There is another
organism on this planet that follows the same pattern. Do you know what it
is? A virus. Human beings are a d |
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a********k
发帖数: 2273 | 8 share any homolgy with mammals |
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l**********n
发帖数: 240 | 9 Josef Martin Penninger
Most significant scientific contributions:
Our basic approach is to genetically manipulate and change genes in mice and
to
determine the effects of these mutations in development of the whole
organism and in
diseases. From these mutations we are trying to establish basic principles
of
development and basic mechanisms of disease pathogenesis. My laboratory
focuses on
heart diseases, autoimmune diseases and cancers, and bone diseases. On all
the listed
contributions below I ... 阅读全帖 |
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j********8
发帖数: 21 | 10 讲的就是基因组印记？
要是这样岂不是将灌水发挥到了极至？
Paternally Induced Transgenerational
Environmental Reprogramming of
Metabolic Gene Expression inMammals
Epigenetic information can be inherited through the
mammalian germline and represents a plausible
transgenerational carrier of environmental information.
To test whether transgenerational inheritance
of environmental information occurs in mammals, we
carried out an expression profiling screen for genes
in mice that responded to paternal diet. Offspring
of males fed... 阅读全帖 |
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t******e
发帖数: 37 | 11 Situated in Southern California, Zymo Research Corporation is a privately
owned biotechnology company that has gained recognition for its top quality
products in nucleic acid purification and analysis. Most recently, Zymo
Research has been noted for its ground-breaking products in the growing
field of Epigenetics by being a recipient of the Frost and Sullivan 2010
North American Product Quality Leadership Award for Epigenetics. For further
information about Zymo Research Corp., please visit www.... 阅读全帖 |
|
p*****m
发帖数: 7030 | 12 这是个问题 说大点就是fish有点不尴不尬的，如果说做保守的东西还不如fly/worm又快
又便宜 reagent也发达；如果说一定要接近人，那fish和mammal还是差的挺多的，就连
frog都不如。说了20多年还是坐定一个所谓的研究vertebrate发育的model来用，我一个
门外汉的感觉就是有点为了搞个model system而model system的意思。
我感觉现在可以慢慢的用fish做一点本来设计之外的东东了 说不定可以更好地利用fis
h的优点。比如说，体外受精这个好处是不是可以用来系统的做epigenetic control,甚
至做reprograming的东西（如果frog不方便的话）？还有，larvae早期脑袋还是透明的
，用来搞很fancy的neural circuitry study会不会很爽？用来做neurodegeneration/r
egeneration会不会很方便？更不要说drug screen了，如果能想出好的assay来潜力非常
大啊，最近几个用fish做的发育之外的screen都很有意思，一个sleep的，一个optomot
or re... 阅读全帖 |
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z*t
发帖数: 863 | 13 circuit有人做了吧，用opticgenetics控制larva的游动方向。
关于epigenetics的话fish有没有imprinting这种现象?要做的话做什么呢?Histone
modification? DNA methylation?Histone modification还好说，因为整套系统相对比
较保守的，但DNA methylation 上因为genome GC含量的不同fish和mammal差的还是挺
多的。您有什么好的idea呢？
又快
一个
fis
/r
非常
optomot |
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p*****m
发帖数: 7030 | 14 我觉得fish做这个最大的好处 就是IVF。从single cell stage开始就能获取到细胞，
以后每个阶段都可以取道细胞。这个在mammal里几乎是不可能的：因为在妈妈肚子里呢。
同样的理由，这几年用拟南芥作epigenetics也是风生水起的。
只是我一点门外汉的理解 仅供参考把。另外你也没得罪我啊:) |
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p*****m
发帖数: 7030 | 15 我觉得这么说吧：现实中大概超过95%的lead compound在preclinical和phase I被kill
掉吧（我记不住了 也许更多）。主要原因一是药代有问题，比如说到不了该去的organ
,或者进了细胞被golgi修饰了被lysosome讲解了,blabla；第二个是safety，直接把动物
搞死了再有用也不行。这两点在cell based screen里都解决不了。
如果用fish替代cell based screen的话，这两方面都会有进步。药代肯定要好得多，至
少细胞内修饰问题应该是保守的；safety方面，这个要看做出来的结果fish和mammal有
多大相关性了。今年fish meeting我看到有两个pharma再尝试validate fish as a tox
icity model，其中一个是pfizer还有一个忘了，还没有什么结果倒是，但是如果相关性
不错的话这方面fish也有优势。 |
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a****d
发帖数: 1919 | 16 genomic imprinting目前确实只在placental mammals 和 flowering plants中有报道。
机制
xenopus |
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p****u
发帖数: 239 | 17 Shinya Yamanaka
The J. David Gladstone Institutes - USA
and
Kyoto University - Japan
Rudolf Jaenisch
M.I.T.
and
Whitehead Institute
U.S.A.
For the generation of induced pluripotent stem cells (iPS cells) from skin
cells (SY) and demonstration that iPS cells can be used to cure genetic
disease in a mammal, thus establishing their therapeutic potential (RJ). |
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t******e
发帖数: 37 | 18 Situated in Southern California, Zymo Research Corporation is a privately
owned biotechnology company that has gained recognition for its top quality
products in nucleic acid purification and analysis. Most recently, Zymo
Research has been noted for its ground-breaking products in the growing
field of Epigenetics by being a recipient of the Frost and Sullivan 2010
North American Product Quality Leadership Award for Epigenetics. For further
information about Zymo Research Corp., please visit www.... 阅读全帖 |
|
z*t
发帖数: 863 | 19 The guy is actually the first to demostrate environment affected mestable
epiallele existed in mammals, the postdoc who work with him is at Baylor now
, he told me about he's not quite in favor of transgenerational epigenetic
inheretance in epidemiological study, he's going to study the early time(
pregnacy/neonatal) will affect the risk of disease by affact the epiallele.
For transgenerational epigenetic inheretance, Yang Shi seems conduct project
with worms,and the recent paper by Oliver Rando... 阅读全帖 |
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v******l
发帖数: 235 | 20 求Paper, 请发到 j********[email protected]. 包子答谢!
PMID: 19546862
[PubMed - indexed for MEDLINE]
Nat Rev Nephrol. 2009 Aug;5(8):441-9. Epub 2009 Jun 23.
The role of transient receptor potential channels in kidney disease.
Woudenberg-Vrenken TE, Bindels RJ, Hoenderop JG.
Source
Department of Physiology, Radboud University Nijmegen Medical Centre,
Nijmegen, The Netherlands.
Abstract
The transient receptor potential (TRP) superfamily consists, in mammals, of
six protein subfamilies, TRPC, TRPM, TRPV, T... 阅读全帖 |
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M*****n
发帖数: 16729 | 21 can't agree more.
I pretty much share the same feeling about sleeping.
just one comment, whether the metabolism of fish is comparable to that of
mammals and human is an open question, so this field needs to be established
first.
的。 |
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k****r
发帖数: 24 | 22 AGENDA – TUESDAY, SEPTEMBER 20, 2011
8:00 a.m. Welcome and Introductions of the 2011 NIH Director’s Pioneer
Awardees
8:30 a.m. Cheng Chi Lee, Ph.D.
University of Texas Health Science Center at Houston
5’-AMP-Induced Deep Hypometabolism in Mammals: Insight Into Its Mechanism
and Potential Applications
9:00 a.m. Karla Kirkegaard, Ph.D.
Stanford University School of Medicine
Quenching Rainbows: Suppressing Diversity in RNA Viruses
9:30 a.m. Evgeny A. Nudler, Ph.D.
New York University Sc... 阅读全帖 |
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c********b
发帖数: 363 | 23 我隐约记得还有几个类似的例子线虫,蚱蜢里面都有small RNA细胞间运输的类似报道(
倒不一定都是吃进去的).
可是这不是在高等的mammal上发现了吗,意义瞬间拔高... |
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x*******e
发帖数: 111 | 24 Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of
cross-kingdom regulation by microRNA
Lin Zhang1,*, Dongxia Hou1,*, Xi Chen1,*, Donghai Li1,*, Lingyun Zhu1,2,
Yujing Zhang1, Jing Li1, Zhen Bian1, Xiangying Liang1, Xing Cai1, Yuan Yin1,
Cheng Wang1, Tianfu Zhang1, Dihan Zhu1, Dianmu Zhang1, Jie Xu1, Qun Chen1,
Yi Ba3, Jing Liu1, Qiang Wang1, Jianqun Chen1, Jin Wang1, Meng Wang1, Qipeng
Zhang1, Junfeng Zhang1, Ke Zen1 and Chen-Yu Zhang1
1Jiangsu Engineering Research Cent... 阅读全帖 |
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c********b
发帖数: 363 | 25 我隐约记得还有几个类似的例子线虫,蚱蜢里面都有small RNA细胞间运输的类似报道(
倒不一定都是吃进去的).
可是这不是在高等的mammal上发现了吗,意义瞬间拔高... |
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x*******e
发帖数: 111 | 26 Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of
cross-kingdom regulation by microRNA
Lin Zhang1,*, Dongxia Hou1,*, Xi Chen1,*, Donghai Li1,*, Lingyun Zhu1,2,
Yujing Zhang1, Jing Li1, Zhen Bian1, Xiangying Liang1, Xing Cai1, Yuan Yin1,
Cheng Wang1, Tianfu Zhang1, Dihan Zhu1, Dianmu Zhang1, Jie Xu1, Qun Chen1,
Yi Ba3, Jing Liu1, Qiang Wang1, Jianqun Chen1, Jin Wang1, Meng Wang1, Qipeng
Zhang1, Junfeng Zhang1, Ke Zen1 and Chen-Yu Zhang1
1Jiangsu Engineering Research Cent... 阅读全帖 |
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c********b
发帖数: 363 | 28 读了全文,觉得最重要的生理条件下这些miRNAs是有功能的这一点没有被很好的support.
当然,也很难.文章都是那chinese的血清,得找不吃大米的人的血清才有系,不然连对照
都没有. : )
你要全文的话给个email.
of
Jing |
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j*****d
发帖数: 787 | 29 如果能证明在mouse中有功能都足够了。还有，有没有更直接的证明这些mir定位于单细
胞内？
support.
★ 发自iPhone App: ChineseWeb - 中文网站浏览器 |
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s******y
发帖数: 28562 | 30 我有，把email address 给我吧
我粗粗的看了一下，数据还是挺多的，文章也比较完整。
当然他们没有特别直接的实验来证明他们测到的miRNA168a是直接来自于食物的
（比方说放射性标记一下那个miRNA168a），但是有一大堆间接证据，比方说进食米饭
之后这个东西在血清里的浓度就上升，进食对照食品之后不上升，但是这个有一个顾虑
就是不能排除是代谢反应。不过，话说回来，这个mrRNA168a应该是不可能来源于人的
基因组的吧？
of
Jing |
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l*****e
发帖数: 138 | 31 大概看了一下，数据很多．不过我第一反应是，为什么他们要用HepG2 这个肝癌细胞株
做体外试验呢？
of
Jing |
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j*****d
发帖数: 787 | 32 His lab should generate transgenic plants, as soon as possible, with RNAi
cassette, targeting oncogene, and feed animal models with tumor.
they
inhibit |
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D*a
发帖数: 6830 | 33 : "most animals at wild die long before they reach the beginning of their
＝＝＝＝
This is true
小鼠在自然界中平均寿命也就六个来月，不可能活到两年多。中国古代女子15岁及笄（
可以嫁人），加上解放前平均寿命30岁的数据，所以能作用于reproduction的基因肯定
是跟寿命有这样那样的联系的。
现在说得通的寿命理论（evolutionary theory of aging），我觉得并不是突变积累和
抗氧化，而是kirwood的disposal soma，由willarms的antagonistic pleiotropy发展
而来，中心思想是能量的分配，这个理论认为，机体soma是用来支持生育的，现在的长
寿实际上是要求用更多的能量来维持机体的功能，所以不可避免要从其他地方（例如生
育能力）调出能量，从这个角度可以理解抑制growth hormone/IGF-1通路，或者
caloric restrition，可以影响机体的能量分配方式。
突变积累和抗氧化，我认为只是作用方式
reproduct... 阅读全帖 |
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D*a
发帖数: 6830 | 34 你说的跟我说的并没有矛盾。
正因为small mammals most die from accidents or predation, not die of aging，
才造成aging不是evoltion考虑的因素，所以没有所谓“长寿基因”，而是因为抑制了
这些通路改变了年轻时期的能量分配方式，从而延长了寿命。
人的特例，williarms也试图解释过，如果幼仔成熟期晚，那么menopause的优势就是让
母亲可以有精力抚养长大最后一个孩子。如果母亲死的时候孩子还没有独立，只能在竞
争中死亡，那么延长生育期也是没有意义了。
evolution的竞争单位是个体（更甚一步是“自私的基因”），不是种群。
aging. |
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f********t
发帖数: 6999 | 35 【 以下文字转载自 Military 讨论区 】
发信人: wayofflying (小破熊), 信区: Military
标 题: 南京大学重大发现 揭露了转基因惊人秘密
发信站: BBS 未名空间站 (Sun Oct 2 23:46:36 2011, 美东)
南京大学张辰宇教授重大发现 “剑指”转基因食物安全(图)
在九月出版的《细胞研究》(Cell research)的一篇研究中，南京大学张辰宇教授课题
组展示了一项非常令人惊奇的发现——植物的微小核糖核酸（microRNA）可以通过日常
食物摄 取的方式进入人体血液和组织器官。并且，一旦进入体内，它们将通过调控人
体内靶基因表达的方式影响人体的生理功能，进而发挥生物学作用。该研究证明植物微
小核糖核酸可能是食物中的“第七种营养成分”（其他六种分别是水、蛋白质、脂肪
酸、碳水化合物、维他命和稀有元素）。
南大教授张辰宇作《21世纪生物科学与生物经济》讲座
原文地址
Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of
cross-... 阅读全帖 |
|
i***R
发帖数: 663 | 36 UT Southwestern Scientist Shares 2011 Nobel Prize in Physiology or Medicine
Dr. Bruce A. Beutler, the new Director of the Center for the Genetics of
Host Defense at UT Southwestern Medical Center, today shared the Nobel Prize
in Physiology or Medicine with two other scientists for their discoveries
in how the immune system works.
Dr. Beutler and Dr. Jules A. Hoffmann of Strasbourg University’s Institut
de Biologie Moléculaire et Cellulaire in France shared half the prize for
their discovery of r... 阅读全帖 |
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h******y
发帖数: 1374 | 37 The Nobel Prize in Physiology or Medicine 2011
shall be divided, with one half jointly to
Bruce A. Beutler and Jules A. Hoffmann
for their discoveries concerning the activation of innate immunity
and the other half to
Ralph M. Steinman
for his discovery of the dendritic cell and its role in adaptive immunity
Summary
This year's Nobel Laureates have revolutionized our understanding of the
immune system by discovering key principles for its activation.
Scientists have long been searching for the g... 阅读全帖 |
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z*t
发帖数: 863 | 38 Beutler得奖，Medzhitov会不会有意见？
http://www.sciencedirect.com/science/article/pii/S1074761309002
Ruslan Medzhitov
This year marks the 20th anniversary of a publication (Janeway, 1989) that
in many ways revolutionized our understanding of the immune system. As part
of the proceedings of the Cold Spring Harbor Symposium on Immune Recognition
, the paper authored by the late Charles A. Janeway, Jr. was not a standard
peer-reviewed publication. In fact, Charlie used to refer to it as “the
best paper I've ... 阅读全帖 |
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D*a
发帖数: 6830 | 39 你以为你真的懂进化论？（十一）
最近关于范美忠的讨论引起了大多数人的兴趣。这不奇怪，因为人类都非常喜欢就别人
的道德水准说三道四。我奇怪的是，这次似乎没人借此机会重提“宗教传统”问题，而
以前经常可以见到这样的解释：中国目前之所以道德沦丧，就是因为中国人没有宗教信
仰。
国外也有很多类似的观点。甚至有很多人认为，进化论是导致人类丧失道德的主要原因。
Actually people in more secular countries appear to behave more morally. And
even if this claim was true, that would not alter the facts or justify
their suppression.
但是，越是世俗化的国家，其国民的道德水准似乎越高。不过，即使真的如此，也不能
改变进化的事实，或者压制关于进化论的讨论和传播。
进化论真的会让人道德败坏吗？很多宗教界人士持有这种观点。可惜的是，他们所能找
到的最好的例子也不过是下面这首歌曲：
这是一支名叫Bloodhound Gang的乐队演唱的一首歌，名字叫做... 阅读全帖 |
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y******8
发帖数: 1764 | 40 People observed some transposon activation in somatic cells. But for germ
cells in higher mammals, it is hard to believe, though.
,
allele
theory.
specific
rapid |
|
P****d
发帖数: 564 | 41 Agreed. The scheme can only work in species with huge population size, or
in an experimental system, large progeny number. That's why I didn't say
human or mammal but meant to discuss it as a general question. :) On the
other hand, I've been wondering about the avalanche of mutations in some
cancer cells. It seems to contradict our intuition about deleterious
mutations, so it might be easier to create survivable cells (emphasis on
cells) with large scale random geneome reorganization?
human... 阅读全帖 |
|
y******8
发帖数: 1764 | 42 The information I have is probably outdated, because I left a evolution lab
long time ago.
In general, cat genus has much less genomic diversity comparing to other
mammals. This might be the reason why there are much more lines of domestic
dogs than domestic cats.
would |
|
m**e
发帖数: 857 | 43 can you believe of it? Just 3 amino acid.
Regulatory evolution through divergence of a phosphoswitch in the
transcription factor CEBPB
Lynch VJ, May G, Wagner GP.
Nature. 2011 Nov 13;480(7377):383-6. doi: 10.1038/nature10595.
Abstract
There is an emerging consensus that gene regulation evolves through changes
in cis-regulatory elements and transcription factors. Although it is clear
how nucleotide substitutions in cis-regulatory elements affect gene
expression, it is not clear how amino-acid sub... 阅读全帖 |
|
T****i
发帖数: 15191 | 44 好像是AACAAC(ATG)。反正与mammal不一样。不过你最好google一下。我也许会记错。 |
|
t*d
发帖数: 1290 | 45 真是有钱人会更有钱
Exercise benefits a variety of organ systems in mammals, and some of the
best-recognized effects of exercise on muscle are mediated by the
transcriptional co-activator PPAR-γ co-activator-1 α (PGC1-α). Here we
show in mouse that PGC1-α expression in muscle stimulates an increase in
expression of FNDC5, a membrane protein that is cleaved and secreted as a
newly identified hormone, irisin. Irisin acts on white adipose cells in
culture and in vivo to stimulate UCP1 expression and a broad ... 阅读全帖 |
|
S*********k
发帖数: 507 | 46 Charnov EL and SKM Ernest. 2006. The Offspring‐Size/Clutch‐Size Trade‐Off
in Mammals. American Naturalist 167:578-582.
q****************[email protected] |
|
l**********1
发帖数: 5204 | 47 Please refer
Harmonics of Circadian Gene Transcription in Mammals
PLoS Genet. 2009 April; 5(4): e1000442.
Published online 2009 April 3
Validation experiments show that 12-hour oscillatory transcripts occur in several other peripheral tissues
as well including heart, kidney, and lungs. These harmonics are lost ex vivo, as well as under restricted
feeding conditions. Taken in sum, these studies illustrate the importance of time sampling with respect to
multiple testing, suggest caution in use of ... 阅读全帖 |
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n***3
发帖数: 663 | 48 Science. 2012 Mar 29. [Epub ahead of print]
GBP5 Promotes NLRP3 Inflammasome Assembly and Immunity in Mammals.
Shenoy AR, Wellington DA, Kumar P, Kassa H, Booth CJ, Cresswell P,
Macmicking JD.
请把paper和supplementary data发到e*********[email protected]
thanks! |
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d*e
发帖数: 843 | 49 One of the leading biological physicists of his generation has died.
Tom Duke was born in Claygate, Surrey, on 14 March 1964 and received a
scholarship to study at King's College School, Wimbledon. A degree in
natural sciences at Emmanuel College, Cambridge (1982-85), was followed by a
PhD in polymer physics at the Cavendish Laboratory (1985-88).
After a short period as a postdoctoral research assistant at the Cavendish,
Professor Duke left the UK to take up a position as a Marie Curie Research
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p*****c
发帖数: 20445 | 50 不知道你怎么判断的？
pubmed search "Jun Lu, mit"，这四篇文章都有Golub TR
http://www.ncbi.nlm.nih.gov/pubmed?term=jun%20lu%2C%20mit
Results: 4
1.
MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte
progenitors.
Lu J, Guo S, Ebert BL, Zhang H, Peng X, Bosco J, Pretz J, Schlanger R, Wang
JY, Mak RH, Dombkowski DM, Preffer FI, Scadden DT, Golub TR.
Dev Cell. 2008 Jun;14(6):843-53.
PMID: 18539114 [PubMed - indexed for MEDLINE] Free PMC Article
Related citations
2.
Impaired microRNA processing enhances ... 阅读全帖 |
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