c******r
发帖数: 889 | 1 self-mummification是早就有的传统了，上千年了。特殊diet,要准备好多年，先限食
脱脂消瘦，再喝利尿泻药一类的草药脱水，某种草药成分可以杀死肠道
细菌，并在体内积聚抗菌。反正是准备死了，什么慢性毒药都可以吃了。死前基本都瘦
的和骷髅没两样儿了。死后身体腐败的几率减小。
细胞95%是水分，脱水就不剩什么了，蛋白质，lipid,fat在那么长折腾过程中消耗掉了
。没有水，细菌是无法大量繁殖的。
不是什么超自然的东西，这么干的佛教徒多。需要很强的意志和自律。
前几年说的香河老人，看那过程就是这样的。她估计是在什么典籍里看到这个记载，方
法，照做了。
常人没理由这么做。因为一些佛徒认为这是修炼成果，所以会这么做。过程比较痛苦。 |
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l*****a
发帖数: 38403 | 2 Explain that there is no simple cell. It has many dependencies, not just
thousands but actually millions and billions of genetic processes including
the very basic system of genes that pass instructions for self-manufacture
of life by that specific form of life -- and perhaps, epigenetic tags that
act in an approximately stepwise process by which genetic information is
thought to be translated into the substance and behavior of that specific
organism.[2]
There is ingestion using an organized and... 阅读全帖 |
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c****t
发帖数: 19049 | 3 ScienceDaily (July 9, 2011) — For the past decade, researchers have tried
to reprogram the identity of all kinds of cell types. Heart cells are one of
the most sought-after cells in regenerative medicine because researchers
anticipate that they may help to repair injured hearts by replacing lost
tissue. Now, researchers at the Perelman School of Medicine at the
University of Pennsylvania are the first to demonstrate the direct
conversion of a non-heart cell type into a heart cell by RNA transfer... 阅读全帖 |
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l**********t
发帖数: 5754 | 4
-- again, Mendel's experiment has nothing to do with Darwin's "Evolution
Theory"(new functions are created from "random"mutation and "natural"
selection.)but inheritance/swaping of existing functions .
--wrong. recombination of chromosome, in most cases, provides variations in
the combination of existing gene set, but not creating new functions. It is
a re-assembly process. The "raw materal" for new functional genes (the lego
building blocks for reassmbly, figuratively speaking), are the DNA/R... 阅读全帖 |
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l**********t
发帖数: 5754 | 5 "甚至都已经开始了纯人工生命了" -- that is not true, and completely
misleading for people w/o basic science trainings.
First, the coding of DNA, the transcription to RNA and the translation from
the genetic codes to functional protein are NOT invented or created by any
human being. It is only discovered and decipered by the biologists thru
decades of hardwork. It is like learning to read a "book" after learning the
phonics. At best they are toddler readers, not the AUTHOR of life.
Secondly, the published resea... 阅读全帖 |
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l**********t
发帖数: 5754 | 6 1. 人是由上帝以『現在形態』造出來 deterministically and intentionally.
2. How God create/achieve creation is subject to scientific research to
explore.
3. 人不是猿類（或其他物種）randomly and spontaneously "演化"而來. Highly
complex biological systems are from intelligent design.
4. Different species share common biological building materials/blocks, from
basicpolynucleotides to aa to guar/lipids, to similar gene encoding, to
high level structures in protein motifs and eventually phenotype. |
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O*****e
发帖数: 114 | 7 ☆─────────────────────────────────────☆
lipid (牛三斤) 于 (Tue Feb 3 20:10:16 2009) 提到:
corresponding authors不负责。
中国这种大实验就是害人。
老板不负责，还名利双收。
【八阕】郑重声明：本则消息未经严格核实，也不代表《八阕》观点。【八阕】一个
劳动人民群众喜闻乐见的好地方:http://www.popyard.org
八阕 http://www.popyard.org 近日，浙江大学药学院博士后贺海波被指作为第一作
者，在国际顶级学术期刊上发表的一系列学术论文涉嫌数据造假、一稿多投等。目前
他已被校方解聘，有人同时披露，在这些论文的作者中还包括中国工程院院士、浙江
大学药学院院长李连达及其课题组的主要成员。
校方认为造假系个人行为，与院士无关。
旅欧学者发现14篇论文有假
揭露院士课题组论文造假的人名叫祝国光，目前他旅居芬兰，为全欧中医药协会联合
会副主席。
昨天，祝国光告诉记者，2008年5月，德国《NSA药理学》杂志刊登以HaiboHe(贺海波
)为第一作者的文章《丹 |
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z***a
发帖数: 8436 | 10 thyroid的正常范围理论来说很广。
TSH从3.5到5都算正常。但正常偏低比如3.5，3.6可能会体温偏低。
hypothyroidism也影响lipids代谢：LDL,TG,TC可能偏高，HDL可能偏低。
女性hypothyroidism严重会影响生育。
过年好，包子拿来，嘻嘻 |
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b***y
发帖数: 2799 | 11 ☆─────────────────────────────────────☆
lipid (老子叫荔枝的时候，还没有超女呢) 于 (Sun Sep 18 22:29:39 2005) 提到:
有个code, 先把一个box分成小盒子 grid,
the number of grid in the x, y, z directions is n_xbox, n_ybox, and n_zbox.
下面的一个code写着
/* pre-computing the inverse distances between grid nodes.
N_sqrts = n_xbox * n_xbox + n_ybox * n_ybox + n_zbox * n_zbox + 1
问题1：为什么要加1？
sqrts[0]=0
for (k=1, k
问题2：这个code的目的很明显是先计算各个grid间的距离的倒数，
所以如果，最小的grid的index是(1,1,1),那末最大的inde |
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e****a
发帖数: 17 | 12 the toxicity depends on which cell line you are using
generally, CaP is less toxic than lipid methods in my hands.
and what construct you are tranfecting in to the cells?
is it lethal to cells? |
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c****r
发帖数: 199 | 15 Clinical Lipidology
August 2009, Vol. 4, No. 4, Pages 501-515
A new model system swims into focus: using the zebrafish
to visualize intestinal lipid metabolism in vivo
Juliana D Carten & Steven A Farber
Please send the pdf to:
a********[email protected]
I really appreciated!!! |
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y****i
发帖数: 2194 | 16 我可以负责任的告诉你 不知道这个是什么囊泡但肯定不是lipid droplet
这囊泡往往是你过分stress 细胞之后出现的
比如加病毒浓度太大，加药太猛之类的
10天够长了 你这个照片看起来就是没有分化
你还不确信的话可以测AP2 或者adiposin, adiponectin, 的mRNA或者蛋白 |
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m*p
发帖数: 226 | 17 Embryonic hearts were co-transfected by RFP and GFP plasmids. Only 20% were
transfected. Boss argued that
all RFP and GFP should co-localized and I said it is impossible, only when
RFP and GFP plasmids are in the
same lipid vesicle and fused with the same cell, then they will colocalize.
She said she was one thousand
percent sure. I can not say more, as this might harm the relationship.
However, I would like to know the truth about this co-transfection. Thanks
for your imput. |
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a******u
发帖数: 211 | 18 Int J Pharm. 2002 Apr 2;236(1-2):125-33.
HEPC-based liposomes trigger cytokine release from peripheral blood cells:
effects of liposomal size, dose and lipid composition.
Yamamoto S, Ishida T, Inoue A, Mikami J, Muraguchi M, Ohmoto Y, Kiwada H.
Please send the paper to e*********[email protected]
thanks so much for your help!!!! |
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s*********a
发帖数: 24 | 19 Nature Reviews Endocrinology 6, 71-82 (February 2010) | doi:10.1038/nrendo.
2009.264
Inflammatory lipid mediators in adipocyte function and obesity
Abishek Iyer, David P. Fairlie, Johannes B. Prins, Bruce D. Hammock &
Lindsay Brown
信箱 c****[email protected] |
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a******u
发帖数: 211 | 20 Biomaterials. 2009 Aug;30(22):3691-701. Epub 2009 Apr 15.
Addressing the problem of cationic lipid-mediated toxicity: the
magnetoliposome model.
Soenen SJ, Brisson AR, De Cuyper M.
Please send the paper to e*********[email protected]
Thanks so much!!! |
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p*****m
发帖数: 7030 | 21 both 我打个简单比方吧 随便想一个例子出来：比如说一个人要找到调节动脉粥样
硬化的gene/drug 思路可以是找个mutant出来(一般是病人研究出来的gene 比如和
LDL代谢有关系的)看看是不是有类似phenotype；同时也可以弄些环境因素比如说猛
吃fat给动物。然后拿到类似phenotype之后简单的当然就是找点candidates看看了
比如上面说的LDL related 或者纤维化相关的SMC相关gene之类的。你要想搞复杂
的可以上screen，但是老鼠你总不能每一只都解剖了看血管把 太慢了；鱼你就可以
直接看血管里面荧光标记的lipid怎么分布之类的。。screen的价钱也要低得多。不
管是drug screen还是genetic screen |
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j********r
发帖数: 156 | 22 Please recommend a lipid for primary MEF transfection. Thanks,
Seems electroporation (amexa) gives the rate around 15%, who might be quite
low. There are many lipo products on the market, just name a few
lipofetamine 2000 (with plus)
Fugene 6
GenePorter
GeneCarrier
DreamFect
Superfect
....
which or what else is best for MEF? |
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w***e
发帖数: 269 | 24 it is better to use the diet from the same company. The reason is that the
macronutrient content of mouse diet (lipid, protein and carbonhydrate) is
controlled, but not micronutrients (minerals etc) nor the sources ( animal
protein vs. soy protein). So i think it is better to use the diet from the
same company. |
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h***a
发帖数: 145 | 25 Hi,
I am wondering if anyone worked on MLE-15 cells transfection with siRNA
before?
What lipid solution did you use and at what confluency point did you
transfect the cells?
Thanks a lot |
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h**********r
发帖数: 671 | 26 As far as I know, hexose fermentation is not a big deal. But utilization of
hemicellulose or lignin cellulose is a problem.
Right now, many pepole are turning to microalgae or some photosynthetic
organims such as cyanobacterium. By the way, lipid production from algae
sometimes is better than alcohol. Some dark horses utilize CO2 under
unaerobic conditions have also been paid much attention.
If you want to work on technolgy, there's much work need to do, such as
harvesting oil or strain isolatio |
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j********r
发帖数: 156 | 27 Admiring yi xia! It seems that your efficiency is much higher than the two
NS papers using non-viral vector. Just curious which MEF did you use (to
enable transfection and drug selection)? I also tried plasmid transfection (
via lipids) in primary MEF (at passage 2), however, the transfection is
extremely low. Thanks for the great information! |
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j**********k
发帖数: 296 | 28 According to Web of Science，他关于lipid metabolism的citations大概2.5k（
postdoc期间的SREBP discovery相关），独立做PI后，关于cell death的citations大
概27k，关于RNAi的大概0.55k。合计应该刚好超过30k。刨去非1st或非通讯作者，
完全contributed by him的在25k以上。 |
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M*****e
发帖数: 279 | 29 Can anyone elaborate on Xiaodong's SREBP?
SREBP transcription factors: master regulators of lipid homeostasis.
Is Xiaodong the first person who found some of transcription factors (like
SREBP) that can be synthesized as transmembrane (or membrane bound)
precursors, but become active transcription factors by intramembraneous
proteolysis?
It is now well-known that intramembraneous proteolysis plays an important
role in some signaling pathways, such as WNT (beta-catenin) and Notch.
If Xiaodong is t |
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l***m
发帖数: 26 | 30 我只知道现在比较热的，预测未来那个好像不是我们该做的！当然有远见很好！
lipid signaling？？
autophagy?
glycosylation?
stem cell related...
每个学科都无法预测吧！因为你不知道未来有什么新发现！ |
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m*********7
发帖数: 5207 | 31 不需要用lipid based transfection reagent。 |
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b******n
发帖数: 82 | 32 http://www.cell.com/abstract/S0092-8674%2810%2901180-3
The ER UDPase ENTPD5 Promotes Protein N-Glycosylation, the Warburg Effect,
and Proliferation in the PTEN Pathway
Min Fang, Zhirong Shen, Song Huang, Liping Zhao, She Chen, Tak W. Mak,
Xiaodong Wang
* Highlights
* The ER UDPase ENTPD5 is upregulated by AKT in PTEN mutant cells
* ENTPD5 hydrolyzes UDP to UMP to promote protein N-glycosylation
* ENTPD5, along with CMPK1 and AK-1, hydrolyze ATP to AMP, increasing
aerobic glycolys... 阅读全帖 |
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h**********r
发帖数: 671 | 33 Accumulation of PHB is very common in the bacteria under imbalanced growth
conditions, especially nitrogen/Pi limitation. This is one of storage
compounds. Furthermore, some algae can produce higher content of lipids
under such conditions. Maybe PHB has other functions. |
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m******5
发帖数: 1383 | 34 A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus
1. Felisa Wolfe-Simon1,2,*,
2. Jodi Switzer Blum2,
3. Thomas R. Kulp2,
4. Gwyneth W. Gordon3,
5. Shelley E. Hoeft2,
6. Jennifer Pett-Ridge4,
7. John F. Stolz5,
8. Samuel M. Webb6,
9. Peter K. Weber4,
10. Paul C. W. Davies1,7,
11. Ariel D. Anbar1,3,8 and
12. Ronald S. Oremland2
+ Author Affiliations
1.
1NASA Astrobiology Institute, USA.
2.
2U.S. Geological Survey, Menlo Park, CA, US... 阅读全帖 |
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S******G
发帖数: 965 | 35 我的一个朋友给了我文章里面的数据。
1. 砷出现在protein，DNA这些里面不能说明他们对machinery和biochemistry有贡献。
换言之，这个细菌也许只用了其他10%的endogenous的100%含磷的agent来进行生化活动
。更退一步来说，如果there had been no phosphorus to begin with, 这些细菌肯定
活不下去。
2. 说明砷和磷一样是砷酸盐的形势存在。很好很强大，还是不能说明问题。
我觉得他们放卫星放得太早了点。
1. When the researchers added radioactively labeled arsenate to the bacteria
’s culture,they were later able to discern its presence in the protein,
lipid, nucleic acid, and metabolite fractions of the cells, suggesting that
arsenic had been incorporated i... 阅读全帖 |
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s********n
发帖数: 2939 | 36 1. 好氧细菌（16S rRNA鉴定）；
2. 不是严格的嗜砷生物（not obligate arsenophile）；
3. 此菌在磷存在的条件下生长得更好；
4. 在As+/P-的条件下，细胞中还是含有一定量的磷（从试剂中带入），但砷大概是磷
的7.3倍（文章中显示砷的测定不是很准确），而且此数量的磷据推测不足以满足细菌
生长的需要；
5. As+/P-条件下生长的细胞比As-/P+条件下生长的细胞大，主要是由于产生了PHB；
6. 作者通过实验证明As分布在细胞的各个部分，包括DNA, RNA, protein, lipids和
metobalites（放射性深和磷的分布）;
7. 作者讨论提到尽管含As的代谢物尽管相对于P而言很不稳定，但他们推测此菌有一套
应对的方法，比如说利用PHB的疏水环境。 |
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m**z
发帖数: 787 | 37 some mass spec data on either small molecules such as ATP, lipids or
macromolecules such as DNA or RNA? |
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k****u
发帖数: 3454 | 38 re
我只看了摘要
摘要里说可以识别water, lipid和protein
那么还有无穷多的metabolites呢，我不是非常清楚raman scattering的原理，但是这
玩意儿的dynamic range如何？还有如何解决low abundance的东西的问题？有没人能科
普下拉曼光谱成像啊。。。 |
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z*t
发帖数: 863 | 39 even if you saw skin it's not enough, how can you distigush different cells
under SRS? By morphorlogy? Some cell are so similar you can only distingush
them by surface marker. Can they distigush these cells by SRS?
One cell can have lot of different metabolites, how can they tell what kind
of substance is there in the cell? They claim they can do cytometry by SRS,
however, you can only distingush by morphorlogy
They're still long way to go. But anyway, that's big advance. Meng Wang in
Baylor has... 阅读全帖 |
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b****n
发帖数: 311 | 40 好像他们的镜子看lipid挺成熟。Ruvkun和他们有合作。 |
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p*l
发帖数: 1359 | 41 After hundreds years of astronomy research, we are pretty sure that the
physics law applies to the whole universe. All chemical elements and the law
of chemical bonds should be universal too.
I think the basic principle of life should be the same too. You need a
genetic material to carry the code of life through generations, a mechanism to harvest and transfer energy, and a chemical structure that separates life from its surrounding. But the recipe for making live may have different versions. L... 阅读全帖 |
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K**********e
发帖数: 188 | 42 不要这么肯定吧。基本物理定律在人类存在的宏观三维宇宙适用，但是对于更高维度就
不知道了。何况还有物力定律失效的
其它宇宙。
至于生命是什么，也要先定义一下。也许有和地球生命完全不同形式的“生命”，只是
地球人观察不到，或者不认为它们
是“生命”。即使是人类认可的生命形式，前几天不是还有砷基生命之说吗。
law
mechanism to harvest and transfer energy,
and a chemical structure that separate life from its surrounding. But the
recipe for making live may have
different version. Live may not be made out by DNA, RNA, ATP and lipids. |
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s******r
发帖数: 2876 | 43 这两天正在看三体，
宇宙是否均匀看来还不能肯定，那物理定律也可能不一定各处都成立。
After hundreds years of astronomy research, we are pretty sure that the
physics law applies to the whole universe. All chemical elements and the law
of chemical bonds should be universal too.
I think the basic principle of life should be the same too. You need a
genetic material to carry the code of life through generations, a mechanism
to harvest and transfer energy, and a chemical structure that separates
life from its surrounding. But the recipe for... 阅读全帖 |
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z**n
发帖数: 22303 | 44 量子力学创立才六十年，经典力学就被敲打的摇摇欲坠。。。
law
mechanism to harvest and transfer energy, and a chemical structure that
separates life from its surrounding. But the recipe for making live may have
different versions. Live may not be made out by DNA, RNA, ATP and lipids. |
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W****7
发帖数: 426 | 45 估计是礼品lipid一类的东西，我做脂肪细胞的时候遇到这种情况太多了，只能多离心
几次，分几次取出来不能怕麻烦。过滤器也是个好办法，不过你样品很少的话过滤器就
不行了，基本上都损失掉了！过滤器后的样品我做过100-200kD的蛋白大体上都没有什
么问题。 |
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n********k
发帖数: 2818 | 46 Never done it but know that People also use peptide or lipids conjugated
siRNA to enhance penetration of cell membrane...Pls keep us posted as to how
it goes...thanks... |
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s******s
发帖数: 13035 | 47 嗯，我没想过这个denature的问题。
对我来说，non-ionic对lipid作用比较强，对蛋白－蛋白作用弱，所以能去除比较弱
的蛋白相互作用。ionic的分离蛋白作用强点，0.1%SDS还是很稀，不至于蛋白全变性，
能够去掉中等的相互作用，并且溶解一下不好溶的蛋白。
ionic
recognizes
these |
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a********k
发帖数: 2273 | 48 they should be doing some biophysics. I know someone doing DNA penetration
and diffusion studies on lipid membranes. |
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