m*********r
发帖数: 2456 | 1 今天浏览了最近的一篇nature paper，“lincRNAs act in the circuitry controlling pluripotency and differentiation” 看
到第二页就发现了一处明显的错误，还是大牛文章呢， 原文描述如下：
Among these lincRNAs, 13 are associated with endoderm differentiation, 7
with ectoderm differentiation,
5 with neuroectoderm differentiation, 7 with mesoderm differentiation and 2
with the trophectoderm lineage (Fig. 3a).
图见附件，显然5个数字，数错3个， 哎～～～ |
|
H****N
发帖数: 997 | 2 Just to counter you argument on eye evolution, Pax6 is required for eye
formation in all animals examined so far, strongly arguing for a single
origin of all eyes during evolution. Of course, further evolution has
happened along different phylogenetic lineages, but no eye has got away from
the basic structure of photoreceptor-pigment cell.
As to your "巧合", you are making many assumptions again. If evolution is
directional as you think, covengent evlution events should not be as rare as
they are.... 阅读全帖 |
|
s******a
发帖数: 472 | 3 http://www.cell.com/molecular-cell/fulltext/S1097-2765(11)00331
Fifty Years after Jacob and Monod: What Are the Unanswered Questions in Molecular Biology?
=====================================================================
Tomorrow's Molecular Biology
Marc W. Kirschner
Harvard University
Today, the term molecular biology has a rather prosaic sound. After all,
there is little in contemporary biology that isn't molecular. It's hard to
remember that 50 years ago, molecular biology was distinctly ... 阅读全帖 |
|
n***w
发帖数: 2405 | 4 Two cell types and the field generally think they belong to the same
lineage. |
|
|
m**e
发帖数: 857 | 6 can you believe of it? Just 3 amino acid.
Regulatory evolution through divergence of a phosphoswitch in the
transcription factor CEBPB
Lynch VJ, May G, Wagner GP.
Nature. 2011 Nov 13;480(7377):383-6. doi: 10.1038/nature10595.
Abstract
There is an emerging consensus that gene regulation evolves through changes
in cis-regulatory elements and transcription factors. Although it is clear
how nucleotide substitutions in cis-regulatory elements affect gene
expression, it is not clear how amino-acid sub... 阅读全帖 |
|
|
P****o
发帖数: 172 | 8 Yongjun Liu, Director, Baylor Institute of Immunology, and vice president of
research. He is a big icon in pDC field. Both he and Chen Dong (discovered
Th17 lineage, Director of immunology section, MD Anderson) are much more
famous than Shu Hongbing in the immunology field. |
|
l*****1
发帖数: 8 | 9 Int J Oncol. 2011 Apr;38(4):911-9. doi: 10.3892/ijo.2011.933. Epub 2011 Feb
2.
Characterization of cytarabine-resistant leukemic cell lines established
from five different blood cell lineages using gene expression and proteomic
analyses.
http://www.ncbi.nlm.nih.gov/pubmed/21290089
email: l*******[email protected]
Thanks a lot. |
|
r********y
发帖数: 18 | 10 个人理解， microglia 应该+/+的吧。因为microglia作为microphage lineage, 是
bone marrow derived（donor）， 而其他的cns细胞是-/-， 是recipient derived
microglia作用的是不够有说服力的。不知道这样理解对吗？ |
|
g********0
发帖数: 6201 | 11 Federal Grant Bar for Neurologist
02/29/2012 David Pittman
A former SUNY neurology department chair has been found guilty of
fabricating data in two published papers, four grants applications, and a
submitted manuscript.
A former State University of New York (SUNY) neurobiology department chair
has been banned from conducting federally funded research for a year and
will have his work supervised for another two years. The ban is the result
of an investigation that found he falsified data in two ... 阅读全帖 |
|
m******5
发帖数: 1383 | 12 我用这个DsRed Express 2做repoter标记lineage，结果杯具了，因为我的绿光filter
是long pass…… |
|
l**********1
发帖数: 5204 | 13 try
Human Phospho-MAPK Array kit
(R&D Systems, Minneapolis, MN).
link:
//www.biocompare.com/ProductDetails/3015549/Proteome-Profiler-Human-Phospho-
MAPK-Array-Kit-NEW.html
cited from 2011 paper:
Activation of p38 mitogen-activated protein kinase by norepinephrine in T-
lineage cells.
Immunology. 2011 Feb;132(2):197-
link:
//www.ncbi.nlm.nih.gov/pubmed/21039464
>Phospho-MAPK protein array
>Following exposure to NE (150 lM, 15 min), total cellular
>protein was obtained using the reagents and proto... 阅读全帖 |
|
i*********0
发帖数: 915 | 14 你可以看看他们的brdu chasing实验，两个paper的结果差别可不是一般的大。
我怀疑NCB是用neonatal的mice做的，而在Cell上是成年小鼠做的，不过cell paper的
figure和他们的结论有点察觉。cell paper的lineage tracing做的很sexy，有tumor，
有人的samples，这个也是他们能上cell的一个原因吧。
NCB上的都是一堆真正做ISC， skin SC的牛人，分析简单，统计的东西稍微弱了一点。 |
|
c********r
发帖数: 1125 | 15 不是这个领域的，但是读过一些这个领域的文章，另外主要的3个实验室boss的seminar
听过2个，瞎说几句，真正内行不要笑话。。。
实际从前两年开始已经有开始质疑lgr5+cell 是isc细胞的了，比如这篇文章。
Nature. 2011 Sep 18;478(7368):255-9.
A reserve stem cell population in small intestine renders Lgr5-positive
cells dispensable.
这篇ncb是hans clevers实验室也就是最早发现lgr5并且建立整个story体系的实验室，
以前他们还做出了非常fancy的lgr5＋细胞的单细胞体外培养出有功能的器官（小肠，
胃。。。）
但是越来越多的证据显然显示lgr5不是唯一的决定性因子，这篇cell的主要卖点觉得就
是这个，所以lrig1＋与lgr5＋的细胞到底是不是一群实际上是非常关键的问题。。。
如果cell的data是确实的话那么lgr5的重要性显然要大打折扣，但也不至于完全颠覆，
毕竟clevers实验室的体外单细胞培养器官再造和动物移植模型已... 阅读全帖 |
|
|
|
f*****f
发帖数: 195 | 18 新闻上报道的再生人类肝脏只是投到了某会议。
PI是Hideki Taniguchi，刚发了一篇类似的，不过感觉很粗糙啊。不知道新闻忽悠了没
，再生的肝脏具体如何.
Transplant Proc. 2012 May;44(4):1127-9.
Highly efficient generation of definitive endoderm lineage from human
induced pluripotent stem cells. |
|
w********e
发帖数: 21 | 19 John Sulston干了两件纯data driven的大事: 线虫的 Cell lineage 和人类（先是线
虫）的基因组。 |
|
u**********d
发帖数: 573 | 20 是否确定是tuj1的信号？可以做做western看能不能在ES的WCE里检测到条带，及大小是
否合适；
是否其它的ES细胞系也是这样？
如果你确实检测到的是tuj1的信号，我怀疑你的ES疯掉了，比如REST没了，可以看看其
它的neural lineage marker信号。
另外，确实有报道说oct4能在NSC中残留的。 |
|
l**********1
发帖数: 5204 | 21 LZ 的细胞培养过程中 有stress 的话
it might happened. (NB: here stress likes oxgen concentration value)
please refer
Fernandes TG et al. (2010)
Different stages of pluripotency determine distinct patterns of
proliferation, metabolism, and lineage commitment of embryonic stem cells
under hypoxia.
Stem Cell Res. 5: 76-89.
citation:
>beta-tubulin (Tuj1) revealed a slightly higher percentage of
neuronal cells at low oxygen tension that
link:
//www.ncbi.nlm.nih.gov/pubmed/20537975 |
|
b*****o
发帖数: 150 | 22 我想通过负筛选去掉正在分化的细胞的某个lineage。
因为是负向筛选需要一个特殊的gene
我只知道的是TK
请问还有什么类似的么 最好是universal自杀gene。 |
|
m******t
发帖数: 109 | 23 RT. 所谓的lineage tracing?/ |
|
O******e
发帖数: 4845 | 24 你的这个思维还停留到lineage的层面上，实际上跟cancer stem cell理论是一致的。
我比较倾向于cancer evolution的理论，认为癌细胞一直在发生着剧烈的变化，这些
变化中最简单的就是基因突变。这所谓肿瘤进化的动力来自于癌细胞所处环境的巨大
变化。
你可能没有关注最近关于cancer genome heterogeneity的一系列文章。其中一篇文章
把一个肿瘤对等切成四份分别测序，结果呢，得到四份区别天大的突变数据，彼此重合
少得可怜。你想想，以后哪怕NGS便宜到是个病人都能做，你该如何做biopsy才能拿到
最有代表性的组织来测序？更悲观点说，你真能找到所谓有代表性的组织么？
基因的表达调控表现在很多层次上，包括基因突变。但肿瘤细胞所在处的周边环境和
彼此之间的interaction都可能改变基因表达的变化－－这些变化很多都不会体现在
DNA水平。 |
|
w***7
发帖数: 1637 | 25 source：http://bbs.netbig.com/thread-2491712-1-2.html
看了一下，今年不错啊，和清华并驾齐驱。
而且下半年我知道的还有两篇NS。
~~~~~~~~~~~~~~~~~
1.
Yuan W, Wu T, Fu H, Dai C, Wu H, Liu N, Li X, Xu M, Zhang Z, Niu T, Han Z,
Chai J, Zhou XJ, Gao S, Zhu B
Dense Chromatin Activates Polycomb Repressive Complex 2 to Regulate H3
Lysine 27 Methylation
Science, DOI: 10.1126/science.1225237, Aug. 24, 2012
2.
Liu B, Du H, Rutkowski R, Gartner A, Wang X
LAAT-1 Is the Lysosomal Lysine/Arginine Transporter That Maintains Amino
Acid Homeostasis
S... 阅读全帖 |
|
w***r
发帖数: 709 | 26 我不是NIBS黑。我以前的帖子里说过NIBS的水平在美国完全是top10的。前面的帖子里
已经解释了我所谓“一般”的含义。也是相对于晓东以前工作的水平和影响。
我不是做Necrosis的，只是觉得它的生理作用和已知的凋亡的意义无法相比。也许只在
某些特殊的细胞和条件下，才会有所谓“可调控的”的坏死。另外线粒体的动态变化和
凋亡的关系已经提出过了，我也不认同“又”开辟了Necrosis的一扇大门（如果你指的
是这个方面）。文章做得很漂亮，尤其是这一篇 Mixed Lineage Kinase Domain-like
Protein Mediates Necrosis Signaling Downstream of RIP3 Kinase。
言语有些夸张和不当的地方见谅，以上为个人观点。
欢迎指教 |
|
z*t
发帖数: 863 | 27 是不是上皮干细胞没有很合适的研究手段
我当时看他和clevers的Lgr5 neutral drift文章感觉好cool
但是后来发现好多Paper用不同marker都argue他们的结论，认为asymmetric是主流。
不过clevers后来说你们用的不是authentic stem cell marker...
Simons应该去祸害血液干细胞，发育的lineage清楚多了 |
|
y*********u
发帖数: 183 | 28 请问有哪些commercial available的质粒好用的？
有人用过如下这个质粒么？
Generation of a double-fluorescent double-selectable Cre/loxP indicator
vector for monitoring of intracellular recombination events.
Pfannkuche K, Wunderlich FT, Doss MX, Spitkovsky D, Reppel M, Sachinidis A,
Hescheler J.
Source
Institute for Neurophysiology, Robert Koch Str. 39, 50931 Cologne, Germany.
Abstract
Here we describe the generation of a double-fluorescent Cre/loxP indicator
system. This protocol involves (i) all cloning steps to generate ... 阅读全帖 |
|
l**********1
发帖数: 5204 | 29 ZZ
幹細胞是一種能自我增生並且
具有分化成ㄧ到數種細胞潛能的細胞。依據它分化
潛能可分為單效性（unipotent，分化為一
種細胞）、複效性（pluripotent，分化成胚胎期
同源細胞）和全效性（totipotent，幾乎可分
化成身體中任何一種細胞）
----
我們稱這些內層細胞為〝複效性的〞（pluripotent）──也
就是他們可以形成多種類型的細胞但是並非
胎兒發育所需要的所有細胞類型。由於其效能並非
完全的，他們不是全效性的也非胚胎本身，
事實上，如果將一個內層細胞放在子宮內，
它並無法形成一個胎兒。
-----
三、細胞療法的細胞來源
幹細胞依其來源可分為胚胎幹細胞（embryonic stem cells）
及成體幹細胞兩種。胚胎幹細胞來自於囊胚的內層
細胞團，是一種複效性幹細胞（pluripotent stem cells）
而成體幹細胞則是由成體各種器官或組織裡所分離
出來的幹細胞。在不同的成體幹細胞中，造血幹
細胞（hematopoietic stem cells）以及
間葉幹細胞（mesenchymal stem cells）一直是研
究的熱門題目，尤其... 阅读全帖 |
|
l*******i
发帖数: 153 | 30 首先有一个概念要明确：目前说的ES细胞，都是in vitro culture的，与in vivo的不
一样。
ES细胞分为naive和prime两个stage
1)naive stage：ES colony在形态上看起来更“致密”，呈三维结构，对胰酶不敏感（
即胰酶消化不会诱导其凋亡）。对小鼠ES而言，naive的ES来源于inner cell mass(ICM
)。此stage的ES self-renewal更迅速，population double大概只需要12-14小时。对
于其self-renewal的维持需要LIF，stat3,BMP4等。雌性小鼠naive ES的另一个显著特
点是X chromosome reactivation,而在其他类型的细胞中有一条X chromosome是失活的
。从全能性角度来讲，naive ES是真正具有全能性的，能形成完整的个体。
2）prime stage：对小鼠而言，其来源于postimplantation epiblast,是naive stage
的下一个阶段，其形态看起来与naive ES明显不一样，呈“二维”结构，即colony... 阅读全帖 |
|
a**m
发帖数: 184 | 31 If you only know how to get sem from the output of a software rather than
really understand what that means (also arrogantly pointed out something
right to be wrong) you should go and do some STAT 101 review. What I said
was right--I was talking about standard error whereas standard error of the
mean is a more specific lineage of the topic.
额地个神啊，请无视这个帖子。。。概念问题不懂的找书，找不着书也可以google个
wiki了解个大概
★ Sent from iPhone App: iReader Mitbbs Lite 7.56 |
|
a**m
发帖数: 184 | 32 If you only know how to get sem from the output of a software rather than
really understand what that means (also arrogantly pointed out something
right to be wrong) you should go and do some STAT 101 review. What I said
was right--I was talking about standard error whereas standard error of the
mean is a more specific lineage of the topic.
额地个神啊，请无视这个帖子。。。概念问题不懂的找书，找不着书也可以google个
wiki了解个大概
★ Sent from iPhone App: iReader Mitbbs Lite 7.56 |
|
s**********y
发帖数: 144 | 33 I found this one :HCN4-H2B-EGFP knock-in mice
used in this paper from Sylvia Evans lab
Islet 1 is Expressed in Distinct Cardiovascular Lineages, Including
Pacemaker and Coronary Vascular Cells
Not sure if it is what u r looking for.
and there is a transgenic HCN4-GFP line
http://www.informatics.jax.org/javawi2/servlet/WIFetch?page=all |
|
Z**********g
发帖数: 222 | 34 确实是超新星。略看过他的一些文章，lineage tracing玩得很好啊，而且还会
modelling。不过结论是挺震撼的，如体内证实cancer stem cell，如不存在
multipotent mammary stem cells. |
|
s***o
发帖数: 11 | 35
同问！扫了眼相关abstract，做lineage tracking的，不知道有何特殊之处？ |
|
g***s
发帖数: 60 | 36 比如说某个小鼠组织由两种细胞组成，
请问有什么方法证明它们之间能或者不能互相转化。 |
|
g*****n
发帖数: 250 | 37 不可能证明＂不相互转化＂。只能证明＂相互转化＂。 |
|
g***s
发帖数: 60 | 38 谢谢这么专业的回答，
简单的说B细胞是mosaic在A细胞之间的，
好处是有分别的Cre line available，不过都不是inducible的。
本来想法是用B cre + floxed dephtheria toxin receptor，这样
用toxin杀死B细胞，看看B细胞会不会新生，毛病是没法知道是不是
A细胞起源的。用A cre也是一样的问题。
不知道有没有什么巧妙的办法来解决这个问题.
reporter |
|
|
|
m******5
发帖数: 1383 | 41 欠揍地说一句。要不你按照自己的需求重新做个ki 老鼠？ |
|
|
m******5
发帖数: 1383 | 43 你再查查是否其中一个line有flipase line instead of cre line? |
|
|
i*****g
发帖数: 11893 | 45 UCLA吴红水平一般
听过她seminar，整个没有光彩
她是靠出国早，混谱系（著名实验室lineage）出来的
宁可饶毅大嘴当PKU 院长 |
|
A*******s
发帖数: 9638 | 46 有人认为是浪费时间和精力。
AAN: Stem Cells Show Promise in Mouse Model of ALS
THURSDAY, Jan. 10 (HealthDay News) -- Implantation of a neural stem cell (
NCS) fraction of induced pluripotent stem cells (iPSCs) shows promise in a
mouse model of amyotrophic lateral sclerosis (ALS), according to a study
released in advance of its presentation at the annual meeting of the
American Academy of Neurology, which will be held from March 16 to 23 in San
Diego.
Stefania Corti, M.D., Ph.D., from the University of Milan, ... 阅读全帖 |
|
L*********y
发帖数: 322 | 47 为什么不能进清北生命联合中心？You can never use papers as the sole criteria
to judge a scientist's overall quality. Looks like SY has a fairly good
training lineage. SY's Ph.D boss used to be a HHMI investigator. Her postdoc
boss at Stanford, Lu Bingwei was Jan YN's postdoc and Lu has bee doing
pretty well in the Drosophila neurobiology field. |
|
b******k
发帖数: 2321 | 48 lineage好又如何啊 SY要不是他老公怎么可能拿到offer...
criteria
postdoc |
|
b******k
发帖数: 2321 | 49 这个很难讲 因为国内和美国看的东西不一样。
国内看paper,看future direction(尤其是ambition),看方向国内缺不缺；
这里看paper,看lineage,看topic热不热sexy不sexy。 |
|
l**********1
发帖数: 5204 | 50 那是 有牛人罩着
就像她师兄 Inoue T 2007-2008 在干啥 他简历上缺少这n Ys 的记录
http://medicine.nus.edu.sg/bch/pi/takaoi/about.html
but
Inoue T and Sternberg PW. (2010) C. elegans BED domain transcription factor
BED-3 controls lineage-specific cell proliferation during organogenesis. Dev
Biol. 338(2):226-36
Green JL, Inoue T and Sternberg PW. (2008) Opposing Wnt pathways orient cell
polarity during organogenesis. Cell. 134: 646-656.
Inoue T, Ailion M, Poon S, Kim HK, Thomas JH and Sternberg PW. (2007)
Genetic Analys... 阅读全帖 |
|
