g*********o
发帖数: 20357 | 1 oo
Organic foods are foods that are produced using methods that do not involve
modern synthetic inputs such as synthetic pesticides and chemical
fertilizers. Organic foods are not processed using irradiation, industrial
solvents, or chemical food additives. |
|
q*********n
发帖数: 103 | 2 借宝地求教如何计算ccd count number
已知 ccd camera的luminance (radiance)或illuminance(irradiance)以及光的光谱分
布，如何计算出CCD在不同积分时间下，光谱峰值的count number。比如知道了相机镜
头表面的照度是10000lux,我怎么估算CCD在不同波长接收到了多少个counts?
我需要计算饱和度。如果有了这个count level,外加很容易测的饱和时的counts,就可
以预算saturation level 了.
多谢不吝赐教 |
|
m******y
发帖数: 26 | 3 宝石级的应该不少了吧：
Gemstones
A colorless faceted gem
Colorless gem cut from diamond grown by chemical vapor deposition
Synthetic diamonds for use as gemstones are grown by HPHT[28] or CVD[84]
methods. They are available in yellow and blue, and to a lesser extent
colorless (or white). The yellow color comes from nitrogen impurities in the
manufacturing process while the blue color comes from boron.[26] Other
colors such as pink or green are achievable after synthesis using
irradiation.[85] Several compa |
|
z*****n
发帖数: 7639 | 4 from wikipedia:
Foods claiming to be organic must be free of artificial food additives, and
are often processed with fewer artificial methods, materials and conditions,
such as chemical ripening, food irradiation, and genetically modified ingre
dients.
It doesn't say OF has to be GMO-free. |
|
w******e
发帖数: 953 | 5 How safe does transgenic food need to be?
Laura DeFrancesco1
Journal name:
Nature Biotechnology
Volume:
31,
Pages:
794–802
Year published:
(2013)
DOI:
doi:10.1038/nbt.2686
Published online 10 September 2013 Corrected online 26 September 2013
Article tools
PDF
How safe does transgenic food need to be?
Download as PDF (812 KB) View interactive PDF in ReadCube
Citation
Reprints
Rights & permissions
Article metrics
Disputes over how to assess a foodstuff's safety continue to play into
public fears a... 阅读全帖 |
|
L*******e
发帖数: 2153 | 6 恩，Gamma Irradiation of C. elegans
然后就screening了，几百个pcr搞定吧。 |
|
O*******d
发帖数: 20343 | 7 It is very easy. Seal food inside a container, then irradiate it with
cobalt 60. |
|
G******U
发帖数: 4211 | 8 Properties of Hydrogen
Hydrogen is a nonmetal. It is placed above group in the periodic table
because it has ns1 electron configuration like the alkali metals. However,
it varies greatly from the alkali metals as it forms cations (H+) more
reluctantly than the other alkali metals. Hydrogen‘s ionization energy is
1312 kJ/mol, while lithium (the alkali metal with the highest ionization
energy) has an ionization energy of 520 kJ/mol.
Because hydrogen is a nonmetal and forms H- (hydride anions), ... 阅读全帖 |
|
|
m***h
发帖数: 23691 | 9 Is it roasted or irradiated? |
|
c*****a
发帖数: 5428 | 10 看到了
说是treated by irradiation |
|
|
f****2
发帖数: 311 | 12 牛奶，谎言与内幕
作者：蒂埃里•苏卡
Thierry Souccar
译者：陈懿祯
出版社：商周出版
出版日期： 2007年10月27日
语言：繁体中文ISBN：9789861249421
牛奶，真的能让你喝出一身病！
没错，牛奶能让你在三十岁达到骨密度的高峰，代价却是中老年以后骨质多孔的风险喝
牛奶就像替细胞安装了加速器，它让你长得又高又壮，却也加速肿瘤的生长！是谁绑架
了你的健康，你还能装作不知道吗？
这本大爆食品工业内幕的书将会使你大开眼界！作者锁定农产食品市场区块的最大种类
——乳制品大胆挑战，揭露这个庞大产业试图隐瞒的内幕。
这本书包含了科学证据和国际级的好资料，很难找到漏洞来攻击作者的论点。通过这本
书将能了解到因过量摄取牛奶中的生长因数所产生的全部坏处，甚至会看到过量摄取乳
制品可能会让骨质疏松症的病情更严重这一情况。作者以研究作为支持，披露乳品工业
和营养学论述是如何运用一些错误的观念来诱导大众购买越来越多的牛奶的。然而，究
竟牛奶的营养价值到底为何？是否真的喝越多越好？这本书也从正反两方面的研究数据
来告诉大家真相。
作者的意图不在于告诉大家不要喝牛奶，而... 阅读全帖 |
|
t**r
发帖数: 82 | 13 这个人一定要求gamma irradiation后，一点点都不能变黄，变脆， 还说Becton
Dickinson syringe 就可以做到。 我心想，BD还不是在中国source， TYYD.
坚决抵制重阳媚外，像gauze swab之类的dressing, 老外的质量不一定比的过国内。又
不愿意多花钱，又要质量好。去die8。 |
|
h********5
发帖数: 386 | 14 蒙古人来了
建奴来了
日本鬼子进村了
－－－－
中国人民历经苦难， 跑的慢的都淘汰了， 跟风也是逼出来的
不过盐不是吃下去， １磅盐加１磅苏打热水泡澡能帮助身体消解辐射毒害， 爱普生盐
浴法。
多吃海菜不吃糖也有用。
The Epson Salt Bath
Here’s the typical Epson salt bath formula for detoxifying the body from
general radiation exposure, such as from Cobalt-60 irradiated food, X-rays,
or air flights where you are subject to high levels of ionizing radiation.
Dissolve 1 pound of sea salt or rock salt and 1 pound of baking soda in a
hot bath -- as hot as can be tolerated -- and soak into the water until t... 阅读全帖 |
|
q**w
发帖数: 782 | 15 http://www.nature.com/nature/journal/v461/n7268/abs/nature08470
Letter
Nature 461, 1265-1268 (29 October 2009) | doi:10.1038/nature08470; Received
17 June 2009; Accepted 27 August 2009
Preserving electron spin coherence in solids by optimal dynamical decoupling
Jiangfeng Du1, Xing Rong1, Nan Zhao2, Ya Wang1, Jiahui Yang1 & R. B. Liu2
1 Hefei National Laboratory for Physical Sciences at Microscale and
Department of Modern Physics, University of Science and Technology of China,
Hefei, Anhui 230026... 阅读全帖 |
|
发帖数: 1 | 16 请诸位帮忙下载一篇文章
我只能看到abstract,本人email:
[email protected]/* */
Degradation of the natural mutagenic compound safrole in spices by cooking
and irradiation
不胜感谢 |
|
i***a
发帖数: 11095 | 17 Dear HOME members,
亲爱的HOME成员：
I hope you and your family and friends are fine.
As a student in Nuclear Engineering and following the Japanese TV and
Internet news almost non-stop since Friday night I would like to write more
specifically about the situation with the nuclear power plants of Fukushima-
1 and 2. I wish I could bring you information and an analysis of the
situation which would help you feeling a little bit safer and also help you
act calmly and wisely.
我希望你和你们的家人及朋友都平安无事。
身为专攻核子工程的学... 阅读全帖 |
|
R****e
发帖数: 704 | 18 稍长。不过很有趣。。。
In the January 2009 issue of PHYSICS TODAY, Philip Duffy, Benjamin Santer,
and Tom Wigley attempted (page 48) to rebut our argument that there is
significant climate response to solar variability (PHYSICS TODAY, March 2008
, page 50). We find their arguments unconvincing.
The composite curve in their figure 1 is the PMOD composite of satellite
data for total solar irradiance (TSI), which has no upward trend for the
period 1980–2000. However, the second well-known composite, ACRIM, do |
|
a****l
发帖数: 6431 | 19 乳腺癌比例高据说是吃奶制品吃的。
现在有个有名的理论是不吃奶制品。
有个英国的女化学教授在自己得乳腺癌后写了这本书, 就是宣传这里理论。
Your Life In Your Hands: Understanding, Preventing, and Overcoming Breast
Cancer
by Prof. Jane Plant
亚马逊上4.5颗星。
我有一封老外朋友转给我的，介绍这本书。
FW: REVISIT: WHY WOMEN IN CHINA DO NOT GET BREAST CANCER!
Why didn't Chinese women in China get breast cancer?
I had collaborated once with Chinese colleagues on a study of links between
soil chemistry and disease, and I remembered some of the statistics.
The disease was virtually non-existent thro... 阅读全帖 |
|
a*******r
发帖数: 7558 | 20 问题：到达地球Top of Atmosphere的太阳黑体辐射产生的辐照率是多少？［W/m^2/nm]
辐照率irradiance＝E
辐亮度radiance=L
黑体辐射辐亮度：L=2hc^2/(lamda^5*(exp(hc/lamda*k*T)-1)
太阳半径 r=6.96e8 m
1AU=1.496e11 m
omega=6.8e-5 sr （在地球看到太阳所张的立体角）
E=L*omega
我把T=5900K代入L再乘以omega后，得到的曲线总比测量曲线高出一截，而“正确”的
应该是基本重合：
如果我用E=L*omega*cos(66.5deg)，就基本重合了。66.5度是地球自转轴倾斜角。
问题是：在top of atmosphere用卫星测量太阳黑体辐照率，应该和地球自传轴倾角无
关吧？ |
|
p****e
发帖数: 105 | 21 实验要用伽玛射线将癌细胞杀死，可是用伽玛射线要很长时间的培训。请问有没有设么
类似手段可以将癌细胞杀死而且保留细胞蛋白和细胞膜的？紫外行不行? |
|
|
|
j********r
发帖数: 156 | 24 要用gamma-ray 作 feeder MEFs for culture of embryonic stem cells. 大家推荐一个
protocol (such as dose and procedure, cells to be kept in pellet with ice
incubation or on tissue culture dish in antibiotics-free medium?) 多谢！ |
|
S********f
发帖数: 36 | 25 If you have access to the following papers, please help me download them and
send to S********[email protected]. Thanks a lot!
1. Photochem Photobiol. 2009 85(6):1364-74.
Near-infrared photoinactivation of bacteria and fungi at physiologic
temperatures.
Bornstein E, Hermans W, Gridley S, Manni J.
2. Lasers in Medical Science. 23 (4), 349-353,
The effects of laser irradiation on Trichophyton rubrum growth
Emre Vural, Harry L. Winfield, Alexander W. Shingleton, Thomas D. Horn and
Gal Shafirstein |
|
c****e
发帖数: 188 | 26 我想做一个insulin的染色，用那家公司的抗体最好？手上有linko公司的guineapig
anti-human insulin，但总觉得特异性太差，背景太高。还有如何养beta cell使得它
能保持分泌insulin的活性？比如，用gelatin, collagen coated plate,或者low
attachment culture in suspension?有没有人试过用irradiated MEF，beta cell会不
会自己形成一个小colony像IPS那样？实验室就我一人做diabetes的课题，没有太多经
验，想跟各位高手交流一下，谢谢！！！ |
|
b****n
发帖数: 311 | 27 老大，你鸣不平也要做点homework吧？
郭岩怎么着都有5篇plant cell. 就算他去年review,去掉2010年的2篇, 那也有3篇
plant cell.
1: Zhou Y, Zhang J, Lin H, Guo G, Guo Y. MORPHEUS' MOLECULE1 is required to
prevent aberrant RNA transcriptional read-through in Arabidopsis. Plant
Physiol.
2010 Nov;154(3):1272-80. Epub 2010 Sep 8. PubMed PMID: 20826701; PubMed
Central
PMCID: PMC2971605.
2: Zhang C, Guo H, Zhang J, Guo G, Schumaker KS, Guo Y. Arabidopsis cockayne
syndrome A-like proteins 1A and 1B form a complex with CULLIN4 and damage
DNA
bind... 阅读全帖 |
|
l******n
发帖数: 298 | 28 谢谢，我是irradiate the mice for 6 weeks and wait for the replenish of the
donor bone marrow. Then perform vessel injury by denudation or ligature(
possibly by perfusion ischemia).
我还是没明白二者在根本上有什么区别。一种方法，不用放射性，直接注射BMMC,做动
物损伤模型。 另一种方法，就是我用的方法，用放射性，注射的也都是donor的骨髓（
全骨髓）没有在体外分化的，然后做动物血管损伤模型。既然第一种方法也挺好，为什
么我老板让我用第二种方法，多花6个礼拜的时间等donor bone marrow repopulate
into the recipients bone marrow site?
你明白我说的吗？我自己都懵了 |
|
p*****m
发帖数: 7030 | 29 我很小白的问 现在治疗白血病的干细胞移植 不就等于把病人的造血干细胞干光（化疗
+放疗） 然后弄新的进去么？所以把造血干细胞都弄死在弄新的进去技术上没有什么问
题。
但是sunnyday说的办法问题在于，你不可能把所有分化后的血液细胞都弄死啊，我也不
知道有什么技术可以实现这个（要是照射剂量能把分化细胞都弄死，这个人就已经被弄
死了）。你irradiation老鼠，弄死的还是只是bone marrow干细胞吧，然后你种新的进
去，因为分化细胞的寿命有限，几天之后血液里的分化细胞都是新的干细胞产生的了，
bingo。但是对于AIDS病人来说，这几天时间足够HIV从原来的分化细胞跑到新的干细胞
里面去了，等于什么也没干。【 在 seesnowman (wachdog) 的大作中提到: 】 |
|
v*********d
发帖数: 382 | 30 50gy/dose/dayX3 这个条件下，你的细胞都被做成做feeder cell了， ：）
一般的细胞10gy下1000 个里活下1个都不错了。 irradiator 都会调试的，不会坏. 你
要不放心，用gamma-h2ax 染一下细胞就知道了 |
|
l**********1
发帖数: 5204 | 31 En
温习一下 其十一年前的豪言 啊
中国科学：显著的发展和严峻的挑战
——历史演变和现状比较
于2001年12月4日 1st version
饶毅
本文在简要回顾中国科学史的基础上，介绍一些近年研究的内容，肯定中国
科学令人乐观的进步，并讨论可能的意义。同时也指出，中国优秀论文总量仍不
到世界的百分之一，低于中国经济在世界所占的百分比、也不能适应中国持续发
展的要求。中国科学的规模需要相当程度的扩大、质量有待进一步提高。中国科
技还存在面临许多问题和挑战。
中国科学历史上的优秀例子
一个国家科学研究状况可以近似地由发表论文的情况所反映。以下，本文主
要从生命科学的研究来讨论中国科学的情况，一方面这是我有一定判断力的领域，
另一方面生命科学是科学技术最重要的组成部分之一，可以反映科学主流。讨论
中国论文发表情况前，先谈两个背景：中国科学的历史情况，优秀科学和著名杂
志的关系。
奠定中国生命科学研究是二、三十年代协和医学院生理系林可胜和生化系吴
宪。他们不仅自己研究出色，而且培养和带领了其他研究者。林可胜在胃肠道生
理和神经生理有优秀工作。1942年，他在中国当选为美国科学院外籍院士，是... 阅读全帖 |
|
o*******a
发帖数: 242 | 32 泥腿子也好，高中生也好。你最好还是听听，三人行也有我师。
In fighting for hepatitis B infection, the fucntion of cell mediated
immunity is important, but they are not as essential as you said. Instead,
HBsAb is the determining factor that eradicates virus from the body. That
you completely ignore the function of HBsAb is really shocking, and appears
like an un-educated paper reading machine.
You forget the common senses.
The common senses are:
1), HBsAb is REQUIRED to eradicate virus from the body.
In people with chornic h... 阅读全帖 |
|
o*******a
发帖数: 242 | 33 泥腿子也好，高中生也好。你最好还是听听，三人行也有我师。
In fighting for hepatitis B infection, the fucntion of cell mediated
immunity is important, but they are not as essential as you said. Instead,
HBsAb is the determining factor that eradicates virus from the body. That
you completely ignore the function of HBsAb is really shocking, and appears
like an un-educated paper reading machine.
You forget the common senses.
The common senses are:
1), HBsAb is REQUIRED to eradicate virus from the body.
In people with chornic h... 阅读全帖 |
|
P****o
发帖数: 172 | 34 如果你的target 细胞是hematopoitic stem cell来源的，也可以试一下HSC
transduciton, 再转到irradiated rag-/- 老鼠。 |
|
H*H
发帖数: 472 | 35 1. 尚没有听说过转基因作物不可控繁殖的例子！你说的这个中国蟹跟兔子正好说明物
种入侵跟转基因没有半毛钱关系，只要这个物种引进后在当地没有天乱而且环境合适，
他就会大师繁殖。无论是蟹，兔子，还是老鼠。。。
2. 是不是organic不是指种子的基因怎么样！而更多是关注作物种植过程中有没有施无
机肥等等。。。。 Organic foods are foods that are produced using methods
that do not involve modern synthetic inputs such as synthetic pesticides and
chemical fertilizers. Organic foods are also not processed using
irradiation, industrial solvents, or chemical food additives. 袁隆平的杂交水
稻就是转基因，只不过当时的科技还不发达，只能从植株水平来筛选，而现在就可以直
接从分子水平来筛选！你可以说杂交水稻都不好吗？现在还有谁会吃几十年前那种又... 阅读全帖 |
|
e*****r
发帖数: 700 | 36
很多转基因的都是生存能力好。那两个例子是自然界的动物，因为换了个环境生存能力
强。我所知道的转基因的生存能力强，这个没问题吧？转基因不就是要多产么？
and
专家您别忽悠我。这里是USDA的说明
Organic crops. The USDA organic seal verifies that irradiation, sewage
sludge, synthetic fertilizers, prohibited pesticides, and genetically
modified organisms were not used.
链接如下
http://www.ams.usda.gov/AMSv1.0/ams.fetchTemplateData.do?templa
4. 担心非转基因作物被转基因作物污染而绝种，就像担心中国55个少数民族被汉族基
因入侵而灭绝一样，杞人忧天！
可能在世界上的某个角落还有，那么成本呢？我吃个黄豆不能让我花熊掌的钱吧？ |
|
e*****r
发帖数: 700 | 37 不要给我搞农粮组织，它提供organic认证吗？我一个普通消费者只知道美国农业部的
USDA ORGANIC 标签。买食品也只看是否有USDA认证标签。
我前面已经贴过一遍了，Organic crops. The USDA organic seal verifies that
irradiation, sewage sludge, synthetic fertilizers, prohibited pesticides,
and genetically modified organisms were not used. 请注意最后这个 "and" 以后
的短句。
原文连接如下。
http://www.ams.usda.gov/AMSv1.0/ams.fetchTemplateData.do?templa
所以你跟我谈其他的Organic的定义，对我没用。
另外，就算前面有些人说种子入境限制不是因为种子本身，而是害虫之类的。那也是因
为害怕本土不能抵抗外来物种的侵入。防止灾害大流行。流行这个现象就是说该物种在
新环境下生存能力强。如果该物种在本土有天敌，进来一点点根本不算什么。
本人和生... 阅读全帖 |
|
d****d
发帖数: 214 | 38 衷心感谢楼上两位的回复。
我用的骨髓是syngeneic，所用的irradiation的dosage是常规的80%，而且是分两次放
射的。另外老鼠骨髓移植后要喝四周的四环素水。另外我用一般的老鼠作recipient一
点问题都没有。所以真不知道是哪里出了问题。
我一般是转3 million BM cells/recipient。你们一般转多少细胞？ |
|
l****j
发帖数: 70 | 39 有两笼基因敲除小鼠，Female，六个月，SPF饲养环境，food和bedding是gamma-
irradiation的，水是autoclave的，
最近刚发现小鼠有脱毛现象，一笼是躯干脱毛，另一笼是头顶脱毛，
应该怎么处理这些鼠？有可能是皮炎吗？ |
|
p****s
发帖数: 3153 | 40 My advisor says peenas a lot. And for 恶俗,
look at the acronym here:
Proton-enhanced nuclear induction spectroscopy
Proton-enhanced nuclear induction spectroscopy (PENIS[1]) is a nuclear
magnetic resonance technique invented by Michael Gibby and Alexander Pines
while they were graduate students in the lab of Professor John S. Waugh at
the Massachusetts Institute of Technology.[2] It was one of the first of
Pines' experiments transferring spin orientation from one atomic nucleus to
another, which ... 阅读全帖 |
|
r*****i
发帖数: 117 | 41 thank all of you,
I discussed with my boss, he suggested me to do the following experiment:
1. measure the density of bone of KO mice, young KO vs WT, old KO vs WT
2. sections for live and spleen to verify the tumor: myeloid sarcoma,
lymphoma or others,
3. transplantation experiment: KO mice as recipient and donor to check
intrinsic or extrinsic influence,
4. Irradiate young KO mice (sub-lethal) to check DNA damage-inducible
changes in bone marrow, sleen, thymus.
5. analyze different sub-populat... 阅读全帖 |
|
s**********d
发帖数: 1694 | 42 T和B是没有了，还剩些什么其他的呢？google了一下，貌似macrophage是
radioresistant的，那DC, neutrophil,NK什么的都还有吗？倒是可以做把实验试试，
又怕recreate the wheel。还有，老鼠照完了会有inflammation吗？那么多细胞死掉了
，不是很容易诱导inflammation 吗？哪位大牛给点拨一下？ |
|
r******g
发帖数: 600 | 43 我没做过live mouse的radiation~ 但是理论上说，HSC失去分化和proliferate能力了
，所以，所有HSC分化出来的细胞应该都不在了： DC，NK，T, B, macrophage~ 当然，
组织和血液中已经有的这些细胞可能死不了~ |
|
s********n
发帖数: 248 | 44 update：
我上周四按照板上的建议，把CFSE浓度降低到0.5uM,10^7cell/ml室温label 8分钟。
昨天取popliteal LN FACS了一下，所有老鼠的都有很多的OTI细胞，也有很好的
proliferation，在wt/ht/mt里边proliferation的区别也看到了。
估计是我之前用的CFSE浓度太高了，T细胞CFSE label以后都死光了。
真心感谢版上大家的帮忙！
需要比较WT和MT的dendritic cell antigen presentation的差异，因为MT的老鼠本书T
/B细胞和wt有差异,所以把老鼠breed 到RAG deficient background里。
实验是这样设计的，把OTI/OTII （2-5×10^6 cell/mouse） 细胞通过静脉注射打进老
鼠体内，一天以后footpad注射OVA+LPS. 再过72小时harvest popliteal lymph node，
看OTI/OTII增殖情况。因为是RAG deficient 老鼠，所以做tail vein injection前没
有irradiate老... 阅读全帖 |
|
M*********l
发帖数: 378 | 45 能不能确认说，荧光的photobleach只发生在excite的光谱波长范围内？
要做一个绿色荧光CFSE染色，然后做gamma ray irradiation，想确认一下gamma ray不
会对CFSE的荧光产生部分淬灭。
谁知道吗？谢谢 |
|
|
n****3
发帖数: 543 | 47 Organic is a labeling term that indicates that the food or other
agricultural product has been produced through approved methods that
integrate cultural, biological, and mechanical practices that foster cycling
of resources, promote ecological balance, and conserve biodiversity.
Synthetic fertilizers, sewage sludge, irradiation, and genetic engineering
may not be used.
see the link
http://www.ams.usda.gov/AMSv1.0/nop
system
to |
|
l**********1
发帖数: 5204 | 48 用GFP UV-corissing immunoprecipitated mRNAs assay
pls refer,
>
GFP-Based Method for Detection of mRNA-Protein Interactions
HeLa cells expressing N- or C-terminally EGFP/YFP-tagged proteins (Table S7)
were induced with tetracycline, irradiated with UV light, and lysed. GFP-
binding protein (GBP; GFP agarose trap, Chromotek)-immunoprecipitated mRNAs
were detected using an oligo(dT)25 probe fused to TRed dye (Sigma).
RNAs coimmunoprecipitated with GFP/YFP-tagged proteins were identified by
RNASeq. D... 阅读全帖 |
|
f******g
发帖数: 1003 | 49 Kit Regulates HSC Engraftment across the Human-Mouse Species Barrier
•Human HSCs engraft efficiently into adult immune-deficient Kit
mutant mice
•Kit mutation enables human HSC engraftment without irradiation
conditioning
•Human HSCs show robust multilineage engraftment and self-renewal
in mice
•Differential Kit expression delineates two subpopulations in the
human HSC pool |
|
z*t
发帖数: 863 | 50 这事和yamanska有本质的不同吧，yamanaka factor几乎立刻被一流实验室独立证明。
和这次n多人重复不出来有本质区别，而且不光是屌丝大牛lab肯定也有在重复。
stress 和stem cell 这个领域以前有人发现Cancer Stem cell本身对irradiation
resistant,这是stem cell 自有性质还是induce的很难说.楼主好像以前也发帖支持过
stap概念？我觉得与其在这里发帖，不如自己去重复（或者换条件）去证明stap这个概
念。 |
|
