q****u
发帖数: 1421 | 1 FACTORS NECESSARY FOR PROGRESS
This talk was given on October 12, 1972, in New York City
During these days here we have had four or five talks on the different
aspects of Spirituality. Our purpose is to progress on the way. To progress
on the way, there are certain things which are very essential. If they are
not there, progress won't be there.
First of all is regularity. As you take food daily for your body, twice or
three times a day, similarly you are here for the main purpose of giving
food |
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w***f
发帖数: 903 | 2 IEEE Transactions on Knowledge and Data Engineering
2.285
ACM Transactions on Database Systems
1.245
其他两个好像没有。
impact factor 绝对不是joke，不要迷信就是了 |
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d******c
发帖数: 2407 | 3 第二个运行结果不对把
is.na(x)[2] <- T 是什么乱七八糟的？这跟x根本没关系，根本没改变x好吧，我运行
结果x也没有变。
而且用T当TRUE是非常坏的用法。
TRUE and FALSE are reserved words denoting logical constants in the R
language, whereas T and F are global variables whose initial values set to
these.
> (x <- factor(c(1, 2, NA) , exclude = NULL))
[1] 1 2
Levels: 1 2
> x
[1] 1 2
Levels: 1 2
> is.na(x)[2]
[1] FALSE
> is.na(x)
[1] FALSE FALSE FALSE
> is.na(x)[2] <- T
> x
[1] 1
Levels: 1 2 |
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I****s
发帖数: 467 | 4 我是新手正在准备BEC的考试, 看到Becker里面的一道例题看不懂, 这里请教一下.
B3-70页的关于AR factoring的例题,
前面都懂, 就是最后算APR的时候看不懂, 为什么是6,800/40,000?
这里6,800是一年的net cost,但这个40,000是一个月里面收到的钱, 前面计算6,800的
时候都是乘以12的,那么为什么这个40,000不乘以12呢? 因为每个月都可以收到40,000
啊, 那么乘以12不是应该的嘛?
我下面把例题作为图片传上来.
多谢各位了. |
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R******s
发帖数: 16 | 5 Hi, All,
I would like to join this family. As a new member, I wish everybody has a
wonderful career journey and would like to share my experience with you in
the future.
I would like to ask help as well, if you have some experience in the loss
development factor (LDF) analysis. Recently I got a project regarding how
to grouping the territorial LDFs. One idea is based on ANOVA, however, I don
't know how to start it. Have you guys has such experience, what do you
usually do?
Thanks a lot! |
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k********y
发帖数: 27 | 6 小弟正在实习，产险的。遇到了一个project，其中一个book of business里面的一个
line是liability的，在选loss development factor的时候，就是那个loss三角形里面
的数据不充足，接近三分之一是没有loss的，也就导致了数据的缺失。给位牛人们谁知
道遇到这种情况的时候该用什么方法选出LDF？先谢谢啦！ |
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r*******n
发帖数: 1739 | 7 industry factor or weighted average |
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s*******0
发帖数: 3461 | 8 where can get the industry factor? |
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p********1
发帖数: 1011 | 9 是不是在一堆variables里先找几个靠谱的，扔进logistic regression里试试。
然后再加进新的variables来试。但此时，看哪个参数知道这个新加进的variable应该
保留呢？ 换句话，看哪个参数知道model提高了？
还有别的方法找risk factors嘛？ |
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d*****r
发帖数: 2583 | 10 【 以下文字转载自 Chemistry 讨论区 】
发信人: agostic (节水期), 信区: Chemistry
标 题: ISI 2006 Impact Factor (top 1-2000)
发信站: BBS 未名空间站 (Tue Jun 19 15:44:02 2007) |
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a****d
发帖数: 1919 | 11 最新一期的Nature paper，reprogramming from fibroblasts to functional neurons
. 一样的套路，不过还是感觉很crazy！
Direct conversion of fibroblasts to functional neurons by defined factors
Thomas Vierbuchen1,2, Austin Ostermeier1,2, Zhiping P. Pang3, Yuko Kokubu1,
Thomas C. Südhof3,4 & Marius Wernig1,2
Abstract
Cellular differentiation and lineage commitment are considered to be robust
and irreversible processes during development. Recent work has shown that
mouse and human fibroblasts can be reprogrammed to a plurip |
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h**********8
发帖数: 650 | 12 More than acetylation.
EMBO J. 2010 Jan 28.
Crosstalk between C/EBPbeta phosphorylation, arginine methylation, and SWI/
SNF/Mediator implies an indexing transcription factor code.
Kowenz-Leutz E, Pless O, Dittmar G, Knoblich M, Leutz A. |
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a****o
发帖数: 1786 | 13 different. anyting involved in transcription can be called transcription
factor. it includes at least activator and repressor. |
|
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d**a
发帖数: 11 | 15 哪里可以搜某个journal的impact factor? 好像有个excel表包括了所有的.谢 |
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g****e
发帖数: 137 | 16 I'm curious about Plos One's impact factor |
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m******o
发帖数: 571 | 17 PLoS ONE's impact factor has been discussed for a while, now it finally came
out.
4.351 is not too bad. |
|
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w******a
发帖数: 1527 | 19 why do you have to use transcription factor rather than normal
differentiation process? |
|
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m******h
发帖数: 28 | 21 Did anybody know if the trans-acting factor binding consensus has direction.
For example, if the Smad3 recognizes the 5'CAGA3' sequence, can it
recognize the 5'TCTG3' sequence? Are there any experimental evidences? Thanks
for answering. |
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t**k
发帖数: 16 | 22 碰到麻烦乐， 老板让我研究研究有什么软件能够推测dna和transcription factor结合
的软件，然后替换dna上面 base SNP
to see if the changing of single base will decrease the binding affinity ?
Help ! Thank you ! |
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m******t
发帖数: 109 | 23 拿到gene array data，找人分析了以下。和我想的不太一样。 我的设想：根据
dysregulated gene，找certain transcriptional factor target gene, 回推
identify TF. is it doable? and how? |
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c********r
发帖数: 1125 | 24 这个chemokine CXCL family factors在发育里面的作用不是什么很新的东西了，在PGC
迁移，肌肉前体细胞迁移，血管发生上面都有报道了。
我记得比较清楚的是在斑马鱼里面的工作，你可以参考:
Siekmann, A.F., Standley, C., Fogarty, K.E., Wolfe, S.A., Lawson, N.D. (2009
): Chemokine signaling guides regional patterning of the first embryonic
artery. Genes & Development, 1;23(19): 2272-7.
Vasyutina, E., Stebler, J., Brand-Saberi, B., Schulz, S., Raz, E. and
Birchmeier, C. (2005)
CXCR4 and Gab1 cooperate to control the development of migrating muscle
progenitor cells.
Genes and Devel... 阅读全帖 |
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c********r
发帖数: 189 | 25 问一个关于蛋白polymerization的问题，一般来说transcription factor polymerization是不是只有两种情况：translation的时候cotranslational dimerizition；或者binding DNA的时候继续polymerize来稳定与DNA的相互作用。
在用overexpression研究这个问题的时候，有没有可能观察到的polymerization是overexpression的artifact呢？ |
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W*******g
发帖数: 68 | 26 自己顶。
另外有没有人知道到哪里可以查到growth factor在水溶液里面的的degradation时间？
谢谢！
precursor |
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D*a
发帖数: 6830 | 27 我有去年的impact factor 不知道怎么给你？ |
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w****o
发帖数: 367 | 29 Journal of Balkan Union of Oncology.
15 1 2010
Title: Dietary factors and gastric cancer risk: hospital-based case control
study
Author: Lazarevic, K.
请发j*******[email protected] |
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s********8
发帖数: 619 | 30 试了下,找出来一堆啊,这个会不会有很多false positive?
有没有人用过这个Mulan:
http://mulan.dcode.org/
It identifies transcription factor binding sites evolutionarily conserved
across multiple species.
但是我没搞明白怎么用啊,请高手指点. |
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s********8
发帖数: 619 | 31 先谢谢楼上各位的回复!找到了一些ChIP的data,照这个找到了我感兴趣的TXN factor的
两个potential binding site. |
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j****x
发帖数: 1704 | 32 transcriptional factors are Double-strand DNA binding proteins |
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P*********y
发帖数: 41 | 33 Recently helped a collaborator in China to revise their responses to
reviewers' comments. I found the Editor-in-Chief asked them:
"To increase the visibility and impact of this study, it is advisable that
the authors search through articles recently published in THIS journal,
XXXXX, and cite 3 to 4 relevant articles published since 2011".
The impact factor of this journal is around 3; seems all are contributed by
the self citation advised by the Editor-in-Chief.
Speechless! |
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p********1
发帖数: 1011 | 34 是不是在一堆variables里先找几个靠谱的，扔进logistic regression里试试。
然后再加进新的variables来试。但此时，看哪个参数知道这个新加进的variable应该
保留呢？ 换句话，看哪个参数知道model提高了？
还有别的方法找risk factors嘛？ |
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z*********8
发帖数: 1203 | 35 我不太懂統計，是不是應該先做相關性study（調查結果和factor independant or not
independent），比如Xsqaure之類的test，如果相關的話，然後再做logistic
regression啊？ |
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t**********1
发帖数: 61 | 36 今天2013 JCR IF终于出来了，今年比较有看头，因为有很多新兴的journal, 它们与老
牌journal之间的竞争是看点。简单说一下我的看法。
1. NPG
Nature publishing groups的Nat sub整体来讲继续高歌猛进，Nat Commun干掉了PNAS
， Nat Genetics/Materials/Biotech/Nanotech持续保持高Impact factor (>30), 有
的甚至比Science和Cell都高。Nat Biotech去年IF波动较大，今年强势反弹。Nat Med
这个老牌杂志现在看来是越来越走下坡路了，但是应该不会跌倒20以下。Nat Climate
Change第一年的IF非常不错，但是上升前景不是十分乐观。Nat Chem Bio和Nat Struct
Mol Bio影响因子下降，前景不如Nat Commun. Nat Neuro中规中矩，表现令人满意。
Nat Methods继续看涨，但继续大幅上升的可能性不大。总体来讲，Nat sub表现十分抢
眼，尤其是Nature Communications, 这个主打m... 阅读全帖 |
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i****g
发帖数: 3896 | 37 JCI JEM不会那么容易就下去的。有意思的是AHA的几个杂志影响因子都持续走高，比如
circulation和circulation research，都在11分以上了，以前circulation是拼不过
JCI的。
期待eLife的影响因子
今天2013 JCR IF终于出来了，今年比较有看头，因为有很多新兴的journal, 它们与老
牌journal之间的竞争是看点。简单说一下我的看法。
1. NPG
Nature publishing groups的Nat sub整体来讲继续高歌猛进，Nat Commun干掉了PNAS
， Nat Genetics/Materials/Biotech/Nanotech持续保持高Impact factor (>30), 有
的甚至比Science和Cell都高。Nat Biotech去年IF波动较大，今年强势反弹。Nat Med
这个老牌杂志现在看来是越来越走下坡路了，但是应该不会跌倒20以下。Nat Climate
Change第一年的IF非常不错，但是上升前景不是十分乐观。Nat Chem Bio和Nat Struct
Mol Bio影响... 阅读全帖 |
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e*****i
发帖数: 149 | 38 啥叫进取心？
把impact factor搞得很高？ |
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S********e
发帖数: 16 | 39 请教一个transcription factor的问题，在做一个转录因子，发现它特异的binding 一
个基因的3'UTR region in vivo, 并且抑制这个基因的表达，想和大家讨论一下这可能
是什么样的机理呢？多谢！ |
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S********e
发帖数: 16 | 40 请教一个transcription factor的问题，在做一个转录因子，发现它特异的binding 一
个基因的3'UTR region in vivo, 并且抑制这个基因的表达，想和大家讨论一下这可能
是什么样的机理呢？多谢！ |
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m******5
发帖数: 1383 | 41 目的蛋白是个transcription factor，表达很低。
看了不少Chip protocol，似乎stringency都很高，貌似都是给 histon之类表达量高的
蛋白用的，有同修接触过类似情况么? |
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p*****h
发帖数: 36 | 42 Serum starvation gives a lower (thus better) baseline, as serum contains
growth factors; also, your cells are generally synchronized if there is a
growth arrest with serum starvation. You can skip the starvation, and
usually you'll also see an increase in phospho-AKT, ERK1/2 etc.
You may try 0.2% FBS 0.1% FBS etc. instead of no FBS at all.
Check the literature about the concentrations used for your particular cell
lines. 10-100 nM is a good start. |
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D*a
发帖数: 6830 | 44 impact factor也next generation了，汗，都玩大数据啊 |
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T*****e
发帖数: 247 | 45 Cite from web of science:
If you have the legacy JCR platform and you are within the recognized IP
range for your institutions access, then shouldn't need need a
personalization password/ID. Clicking the Journal Citation Reports link on
the top banner of Web of Science should take you to JCR. You can also try
this direct URL. (Which will only work on campus.) The 2013 Impact factors
should be loaded later today.
http://webofknowledge.com/jcr
看来就是今天了。跟我也没啥关系。纯八卦。 |
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m******5
发帖数: 1383 | 46 如题。
用CMV启动子over express一个transcription factor |
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n********e
发帖数: 1630 | 47 有个课题想讨论一下，有个蛋白可以bind nucleic acid， 想看看蛋白跟DNA complex
的结构，这个蛋白的那些部分是跟DNA有接触，specficity
查了一下，很多transcriptional factor - DNA结构都是在solution里面解的
我想问问能用cryoEM解吗
EM能看到蛋白bind DNA，但是要借结构的话，population的heterogeneity如何解决？
用短的nucleic acid，只让一个蛋白bind一段吗
DNA是flexible，弯来弯去的，能解吗？ 单个蛋白分子，complex是可以的
谢谢 |
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