m******t
发帖数: 109 | 1 拿到gene array data，找人分析了以下。和我想的不太一样。 我的设想：根据
dysregulated gene，找certain transcriptional factor target gene, 回推
identify TF. is it doable? and how? | p****z
发帖数: 31 | | M*******s
发帖数: 108 | 3 there are transcriptome data
which TF will transcribe which gene, and also combinatorial effects of TF.
you can just use that kind of data to pull out your genes then you may get
TFs
but I doubt it is convincing enough for publish if without ChIP data
you may carry some further study. For instance, build up the regulatory
network first, then find the dysregulated genes from upstream to downstream
in the network, then you may find some trace of the dysregulation. Generally
, upper-stream usually determines more on original dysregulation...truly
dysregulation (or I prefer heterogeneity) could accumulate by regulatory
signals of time course. | j*p
发帖数: 411 | 4 Try GSEA, see which TF is enriched in your dysregulated gene sets | a********n
发帖数: 844 | 5 用chip把在某个promoter上组装的TR弄下来mass spec一下 |
|
