m******t
发帖数: 109 | 1 i want to know the digestion buffer for genotyping that doesnot purify
genomic DNA.i forgot it. let me know. thx. |
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b******r
发帖数: 111 | 2 These days, almost make me desperate to death. As a 2-year post-doc,who did
lots of cloning, I fail to do such an easy work. Isolate mouse genomic DNA
by phenol/chloroform,75%ethanol precipitation. Then digest by HindIII
restriction enzyme as follows:
genomic DNA: 2 ug,1ug,and 0.4ug;
HindIII enzyme: 70 units(3.5ul,and different units);
Buffer: 5 ul
add water to 50ul
37 degree for 10 hours. Run gel.Genomic DNA is still a strong band, not
smear at all. I have try HindIII from NEB and Roche com... 阅读全帖 |
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b******r
发帖数: 111 | 3 I have done '用chloroform 再洗一次，75% ethanol 再沉淀。',but yield is too
low.
Can I do this:
add NaAc(0.1 volume of genomic DNA), mix,then 2.5 volume of 100% ethanol to
precipitate again? The question is: does a little NaAc still stay in
purified genomic DNA and inhibit digestion of HindIII? |
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g*****n
发帖数: 250 | 4 The question is: does a little NaAc still stay in
purified genomic DNA and inhibit digestion of HindIII?
Maybe. You can use a large volume (e.g., 0.5 ml) of 70% ethanol to wash the
DNA pellet. |
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y****6
发帖数: 196 | 5 I need to digest an antibody into small fragments. Which protease is the
best? Trypsin?
Thanks a lot |
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h*******b
发帖数: 275 | 6 Physiology and Pathology of Membrane Digestion，作者是Ugolev, A. M.
链接http://link.springer.com/book/10.1007%2F978-1-4757-0028-2
不知道谁能下到不？
下到的话麻烦发我邮箱[email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.getElementsByTagName("script");l=b[b.length-1].previousSibling;a=l.getAttribute('data-cfemail');if(a){s='';r=parseInt(a.substr(0,2),16);for(j=2;a.length-j;j+=2){c=parseInt(a.substr(j,2),16)^r;s+=String.fromCharCode(c);}s=document.createTextNode(s);l.parentNode.replaceChild(s,l);}}catch(e){}})... 阅读全帖 |
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b****o
发帖数: 806 | 7 我们想买一些 CEM Microwave Digestion System 的耗材，但是我们的折扣很差，哪位
大侠可以提供好的折扣， 请联系，谢谢了！！ |
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f******h
发帖数: 609 | 8 Thanks a lot, please check for the 5Baozi.
I have the 6page paper. I want the 2page digest from the conference CD...
Thank you very much anyway. |
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w*******y
发帖数: 60932 | 9 Discount Mags
Popular Science
$4.99 per year (up to 4 yrs) w/ code 5001
Golf Digest
$4.99 per year (up to 3 yrs) w/ code 5001
Prevention
from $3.49 per year with code 5001
3 yrs $10.47 ($3.49 per yr)
2 yrs $6.98 ($3.49 per yr)
1 yr $3.99
|
|
w*******y
发帖数: 60932 | 10 Discount Mags
Reader's Digest Magazine Subscription
$3.99 per year with code 9406
up to 4 years
|
|
|
|
|
w*******y
发帖数: 60932 | 14 DiscountMags
2-pack Magazine Bundles $10 each
use code FIREWORKS at checkout
Women's Fitness Pack
Select from the following mags:Shape, Fitness, Weight Watchers, Oxygen
Men's Fitness Pack
Select from the following mags:Maximum Fitness, Flex, Men's Fitness, Men's
Journal
Science & Technology Pack
Select from the following mags:Wired, Popular Science, Discover, Science
Illustrated
Food & Cooking Pack
Select from the following mags:Savuer, Taste of Home, Every Day with Rachel
Ray, Clean Eating
Spor... 阅读全帖 |
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n**4
发帖数: 719 | 17 2楼进化树能展开说一下吗？
我有一个解法 记得是版上某大牛做的
basic idea: use a hash function to get a digest of a tree by hashing its
child subtrees' digests:
digest(tree)=0 if tree is empty
digest(tree)=hash(digest(LeftChildTree),digest(RightChildTree),rootVal)
therefore, this problem can be solved by a post-order traversal. On the
traversal, all the subtrees are digested and registered. If a match occurs,
the roots of both trees and their size are compared and recorded.
the hashTable_get/put functions need the tree digest... 阅读全帖 |
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G**********e
发帖数: 11693 | 18 几乎零卡路里，低GI，粗纤维，抑制食欲，天然的肠胃清道夫，清肠解毒，降血脂血糖
，绝对的好东西
Amorphophallus konjac is a large, perennial exotic Asian plant. It grows a
single, elongated center called a corm and a single large leaf that wraps
around it. The corm is the part of the plant that is used, as well as its
tuber, which is commonly called the konnyaku potato. Glucomannan is its
scientific name, and its other common names include konjac mannan, devil's
tongue, konjac, elephant-foot yam, snake plant and konnyaku.
Significance
Amorphop... 阅读全帖 |
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L*********u
发帖数: 57 | 19 大家有没有空读读这篇英文，提点建设性的意见？
Have you got wrinkles? We all get them when we age. A close look at them
reveals that the wrinkle area has too much skin with very little muscle
underneath. If you want to get rid of wrinkles you can have plastic surgery
to remove the extra skin or you can try to eat a diet to build muscle. If
you decide to build more muscle, the best things to eat are protein and meat
. However protein and meat are not easy to digest. The best time to eat them
is in the winter. Let me exp... 阅读全帖 |
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L*********u
发帖数: 57 | 20 Have you got wrinkles? We all get them when we age. A close look at them
reveals that the wrinkle area has too much skin with very little muscle
underneath. If you want to get rid of wrinkles you can have plastic surgery
to remove the extra skin or you can try to eat a diet to build muscle. If
you decide to build more muscle, the best things to eat are protein and meat
. However protein and meat are not easy to digest. The best time to eat them
is in the winter. Let me explain why.
We take food ... 阅读全帖 |
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b**********1
发帖数: 190 | 21 http://www.coliccalm.com/baby_infant_newborn_articles/baby_coli
What are the Symptoms of Colic?
It is not uncommon for newborn babies to go through periods when they appear
abnormally irritable or seemingly cry for no reason. However, if you
suspect your baby is suffering from colic, you may look for the following
symptoms:
cries vigorously for long periods, despite efforts to console
symptoms occur around the same time each day or night, often after meal
times, and usually ending as abruptly as... 阅读全帖 |
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z********0
发帖数: 9013 | 22 http://stronglifts.com/milk-post-workout-build-muscle-gains/
Post workout nutrition is crucial for muscle recovery & rehydration. The
common advice is to have whey after a weight lifting session because it’s a
fast protein. You want to get protein in your muscles as fast as you can for
recovery.
Truth or marketing from supplement companies? Fact is that many weight
lifters have used milk as a post workout drink for years. After reading this
post you’ll know why it’s the ultimate post workout foo... 阅读全帖 |
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c**********0
发帖数: 836 | 23 Update: added English report link
中国年轻父母追捧的雅培奶粉竟然完全不符合国际标准，也未达到中国国家标准。昨天
，第三方机构香港CERResearch公司发布报告称，其送检了从中国市场购买的六个品牌
的婴幼儿配方奶粉，检测结果均不合格，雅培样品是最差的。
□检测结果
六品牌均不合格雅培喜康宝最差
CERResearch从事中国市场的调查和研究，公司 CEO Graham Earnshaw(晏格文)昨
天接受记者采访时称，公司日前从上海和香港购买了六个品牌的婴幼儿配方奶粉，邮寄
到德国一个世界一流的专业检测食品安全的实验室进行成分检测和分析。
“目前，这些样品的检测数据都出来了，我们将这些数据提供给专家，由他们做出
判断”，晏格文表示，目前专家的态度是，检测的6个样品中，没有一个符合中国国家
标准。其中，雅培的样品被检测出是送检样品中最差的一个，完全不符合国际标准，也
未达中国新国标。
>>雅培样品问题
酪蛋白太高疑高温处理
报告指出，根据中国标准，婴幼儿配方奶粉中乳清蛋白与酪蛋白的最低比例不能低
于60%，而雅培的喜康宝一段的最终检测结果为41%... 阅读全帖 |
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q*d
发帖数: 22178 | 24 ☆─────────────────────────────────────☆
swjtuer (swjtuer) 于 (Tue Mar 30 23:56:30 2010, 美东) 提到:
星期一
早餐 牛奶，煮鸡蛋，玉米馒头，榨菜
早点 芦柑
午餐 中大：绿豆饭，木须肉，海米冬瓜，棒骨菠菜汤
小婴：绿豆饭，三叶瓜炒肉，海米冬瓜，棒骨菠菜汤
午点 冰糖山楂水，蔬菜饼
晚餐 棒骨冬苋菜瘦肉粥，卤肉秋叶夹馍
星期二
早餐 牛奶，蒸鸡蛋，枣泥糕
早点 香蕉
午餐 中大：胡萝卜饭，芹菜炒牛肉，珊瑚豆腐，棒骨凤尾汤
小婴：胡萝卜饭，芹菜炒肉，珊瑚豆腐，棒骨凤尾汤
午点 果汁，卤猪肝
晚餐 豌豆肉末焖饭，番茄虾皮紫菜汤
星期三
早餐 南瓜粥，卤蛋，如意肉卷
早点 脐橙
午餐 中大：金银饭，咸烧白，海米儿菜，棒骨冬苋菜汤
小婴：金银饭，樱桃肉，海米儿菜，棒骨冬苋菜汤
午点 牛奶，蛋卷
晚餐 米饭，蒜苔炒肉，芙蓉蒸蛋，棒骨海带萝卜汤
星期四
早餐 牛奶，蒸鸡蛋，奶香馒头
早点 苹果
午餐 中大：南瓜饭，什锦鱼丸，韭菜炒豆干，番茄菌类鱼汤
... 阅读全帖 |
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c*******e
发帖数: 814 | 25 【 以下文字转载自 Environmental 讨论区 】
发信人: coliphage (coliphage), 信区: Environmental
标 题: 审稿机会 for Water S&T
发信站: BBS 未名空间站 (Tue Oct 22 09:58:56 2013, 美东)
环境工程方面的
manuscript tile及abstract如下：
有兴趣请发你的brief description of qualification及简历至我的邮箱：pfremail@
yahoo.com
Thanks，
Survival of Antibiotic-Resistant Bacteria in Livestock Manure during
Mesophilic Anaerobic Digestion and Electrochemical Disinfection
This is the abstract:
In this study, survival of antibiotic-resistant bacteria in dairy and beef
manures d... 阅读全帖 |
|
c*******e
发帖数: 814 | 26 manuscript tile及abstract如下：
有兴趣请发你的brief description of qualification及简历至我的邮箱：pfremail@
yahoo.com
Thanks，
Survival of Antibiotic-Resistant Bacteria in Livestock Manure during
Mesophilic Anaerobic Digestion and Electrochemical Disinfection
This is the abstract:
In this study, survival of antibiotic-resistant bacteria in dairy and beef
manures during batch mesophilic anaerobic digestion and electrochemical
disinfection process was investigated for the reduction of environmental
pollution. Tested m... 阅读全帖 |
|
s******t
发帖数: 1672 | 27 要求如下。
最主要的两点要求：
1。 有高压操作的经验。 我们的反应器可能要用到4000psi。
2。 要熟悉自动化控制的设备，仪器，软件等。
另外
3。 最好有根据操作条件，设计过程，选用设备的经验
4。 公司可办 H1b。工作地点在 Phoenix AZ
如果感兴趣，发信给HM：
或站内联系我也可以。
多谢！
=========================================================================
Job Title: Process Engineer, W2E
Department/Location: Engineering – Chandler, AZ
Reports To:
Date: 3/28/12
Purpose:
This position is responsible for optimizing the operation of a waste-water
to energy dig... 阅读全帖 |
|
s******t
发帖数: 1672 | 28 要求如下。
最主要的两点要求：
1。 有高压操作的经验。 我们的反应器可能要用到4000psi。
2。 要熟悉自动化控制的设备，仪器，软件等。
另外
3。 最好有根据操作条件，设计过程，选用设备的经验
4。 公司可办 H1b。工作地点在 Phoenix AZ
或站内联系我也可以。
多谢！
=========================================================================
Job Title: Process Engineer, W2E
Department/Location: Engineering – Chandler, AZ
Reports To: Date: 3/28/12
Purpose:
This position is responsible for optimizing the operation of a waste-water
to energy digester system.... 阅读全帖 |
|
q****i
发帖数: 6923 | 29 white ppl are born to have much more stronger digestion system than chinese.
I think that the bad eating habit could be the reason for the weaker
digestion system of Chinese. IF you observe carefully, you will find that
some white ppl have very good eating habits, they never over eat and they
exercise a lot.Surely, exercise will help with the digestion.The good
digestion system may evolve after many generations.
Usually ppl who eat together will all have H. pylori if one of them has. My
mom has ... 阅读全帖 |
|
s******t
发帖数: 1672 | 30 要求如下。
最主要的两点要求：
1。 有高压操作的经验。 我们的反应器可能要用到4000psi。
2。 要熟悉自动化控制的设备，仪器，软件等。
另外
3。 最好有根据操作条件，设计过程，选用设备的经验
4。 公司可办 H1b。工作地点在 Phoenix AZ
如果感兴趣，发信给HM：
或站内联系我也可以。
多谢！
=========================================================================
Job Title: Process Engineer, W2E
Department/Location: Engineering – Chandler, AZ
Reports To:
Date: 3/28/12
Purpose:
This position is responsible for optimizing the operation of a waste-water
to energy di... 阅读全帖 |
|
s******t
发帖数: 1672 | 31 这两天 HM 已经收到了一些简历。面试从明天也开始要了。
祝好！
===============
要求如下。
最主要的两点要求：
1。 有高压操作的经验。 我们的反应器可能要用到4000psi。
2。 要熟悉自动化控制的设备，仪器，软件等。
另外
3。 最好有根据操作条件，设计过程，选用设备的经验
4。 公司可办 H1b。工作地点在 Phoenix AZ
如果感兴趣，
或站内联系我也可以。
多谢！
=========================================================================
Job Title: Process Engineer, W2E
Department/Location: Engineering – Chandler, AZ
Reports To:
Date: 3/28/12
Purpose:
This position is responsible for optimizing t... 阅读全帖 |
|
w*******y
发帖数: 60932 | 32 Surprised none of you early birds posted this. Great deal on an electric
sharpener specifically designed for Shun Knives. Sharpens at a 16-degree
angle. If you bought in on that Williams-Sonoma deal last month, you may
want this. Goes for $80 on Amazon and good reviews.
Link:
http://home.woot.com/sale/kai-shun-electric-knife-sharpener?utm Digest&utm_campaign=ed9508f2ab-Daily Digest - 20120116 - Home.Woot&utm_medium=email#tracked
#tracked" rel="nofollow" target="_blank" onclick="_gaq.push(['_... 阅读全帖 |
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j********x
发帖数: 2330 | 33 greedy只有在每门只是至多另一门课的先修课程的情况下是正确的，证明在下面的论文
里：
http://www.jstor.org/stable/pdfplus/167050.pdf?acceptTC=true
845页以后的部分，比较麻烦。
如果是任意的先后关系，则问题是np hard。经典的scheduling问题。
一般的算法分析可以大概猜出这道题在一般情况下是np hard这个结论。greedy应该是
一个很明显的近似算法，至于其他就看面试官心情了。
另外lz面什么方向的职位，怎么问这么奇葩的算法。。。
后面的document查重我以前问过类似的题，我提到的做法是从文件里的任意位置取少量
字符做digest。用digest做hash key，将放到hash table里，另设一个
table存放（这里假设ID是线性排列的整数，如果不是同样做hash table
）。找重在两个表里搜索就行。
假设160bit，20byte的digest，ID为4byte，每个表大概是10G内存，总共消耗20G，看
情况吧。
数据量更大就上mapred... 阅读全帖 |
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d**********0
发帖数: 13081 | 35 Breakthrough In Probiotics Has Significant Weight Loss Effects
October 28, 2014 by admin
PBIHealthcare experts continue to regard probiotics as one of the most
powerful tools in the management of everything from constipation and
bloating to diarrhea and skin health.
Historically, yogurt has been a primary source of probiotics, but yogurt
products loaded with sugar have their own health implications for the tens
of millions of Americans who are trying to lose weight. Sales of the
healthier Greek-... 阅读全帖 |
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M**********e
发帖数: 4237 | 36 PLAYERS!
Competition is stiff!
Give yourself the edge
and
Help yourself to be the very best you can be!
What you put into your body does affect your energy level
Read the great advice and tips below.
With thanks to Health Consultant
Diana McKenzie, RN
Soccer is a fast paced, intense, competitive sport, and the demands on a
players’ body can be incredible. During a game, a player is in constant
motion
for 30-45 minutes at one time, depending on age and level of play, followed
by
a 10 minute break... 阅读全帖 |
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y***r
发帖数: 16594 | 37 http://sangnabar.org/?fromuid=43593
大家看看，这都是啥乱七八糟的？
【良主任评】上天眷顾云雨共度穿梭在我记忆中的双飞姐妹花??（21P+录音） 一
刀 阅至1043楼 ...23456..143 蜀之赵云 2012-06-01 14297202
wdl7772012-10-04 10:21
【良副书评】丝袜口暴风情万种女医生（5P露脸露奶） attachment agree 專業打
飛機 阅至1084楼 [金钱 +18] ...23456..132 李少白 2012-06-08 13198001
连战2012-10-04 04:52
【良专评】我参加户外爬山，却爬到了良家床上去了！［8P＋录音］ attachment
digest agree 小狼探花 阅至831楼 [金钱 +10] ...23456..129 风花雪月 2012-
04-07 12846450 crespo1312012-08-31 15:20
【良巡评】原创和吉林少妇... 阅读全帖 |
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c********x
发帖数: 1565 | 40 觉得这篇问答写的不错，regarding CRF， phosphate level and raw feeding :
以下文字转载自
http://feline-nutrition.org/answers/answers-kidney-disease-phos
----------------------------------------------------------------------------
------
I have a cat with kidney disease and I have heard that reducing phosphorus
intake is important for cats with kidney problems. Is it safe to feed a cat
with chronic kidney disease a raw meat diet? Doesn't it have too much
phosphorus? My cat hates the prescription diet and I am looking... 阅读全帖 |
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|
w*******y
发帖数: 60932 | 42 Link:
http://www.amazon.com/Vibrant-Health-Green-Vibrance-Family/dp/B
$41.90 with Subscribe & Save - Free Shipping
The cheapest I've seen this for is the current price, which is 49.29, and
this is the first time I've seen it eligible for subscribe & safe... 25
billion probiotics per serving.
Excellent product - check out the reviews.
Green Vibranc
The Greatest Food on Earth
* The #1 Green Food in the Country!
* Nutritional Powerhouse
* Loaded with trace nutrients
* Supports healt... 阅读全帖 |
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k****u
发帖数: 1686 | 43 2012年专四常见词辨析：“吸收”与“荒谬” 东方网教育 2011年08月24日15：03 来
源：考试吧 absorb，suck，digest，incorporate
这些动词均有“吸收”之意。
1.absorb ：普通用词，词义广泛，既可指吸收光、热、液体等具体东西，又可指
吸收知识等抽象概念的东西。
eg: 1.Dry earth absorbs water quickly.
干土吸水很快。
2.You should absorb lots of knowledge when you are young.
你应该在年轻的时候多吸收知识。
2.suck ：作“吸收”解时，可与absorb换用，但还可有“吮吸”之意。
eg: 1.He sucked hard for air to keep breathing.
他拼命吸气以维持呼吸。
2.The baby was sucking its mother's breast.
婴儿正在吮吸母乳。
3.digest ：侧重在消化道内改变食物的化学结构后被人体吸收。
eg: 1.Cheese doesn't digest easily.
... 阅读全帖 |
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q****i
发帖数: 6923 | 45 i think that his body is quite weak. you need to strengthen his body first.
One of the choice will be food. Some food could help with digestion. After
good digestion system is developed, he will have energy in the daytime and
at the same time rest well at night.
The food that I recommend is nuts such as cashew and macadamia. Also, you
can try red dates and cinnamon. When you cook, especially breakfast and
lunch, use more ginger, onion and garlic, you can ever try Five spices for
flavor, they wil... 阅读全帖 |
|
g********e
发帖数: 1638 | 46 195 黄佳
(chem.) 男 1980/11/23 同济大学 工程与材料科学 2009年05月毕
业于[美国]约翰霍普金斯大学 [美国]马里兰大学 博士后研究员/Research
Associate
这位自己有简历在网上，
http://www.linkedin.com/pub/jia-h/8/b10/3b9
第一作者文章很少，有一个JACS，
Publications
Applications of ZnO in organic and hybrid solar cells
Energy & Environmental Science
2011
Authors: Jia H.
(1) Huang, Jia*; Yin, Zhigang; Zheng, Qingdong. Energy & Environmental
Science. (2011) Accepted, *corresponding author
Polymeric Semiconductor/Gra... 阅读全帖 |
|
c******t
发帖数: 1733 | 47 The first time I looked into an open chest cavity at a heart muscle working
to pump blood through the body, I was brought up short by the tragic
graffiti of atherosclerotic plaque. This waxy goo, often found in overweight
people, builds up in the vessels surrounding the organ in which,
philosophers tell us, the soul lives. But I quickly pushed any such
distracting thoughts from my mind. I was learning to operate and was frankly
thrilled at the prospect of harvesting a leg vein to bypass the bloc... 阅读全帖 |
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m******i
发帖数: 9479 | 48 今年的新paper
http://www.ncbi.nlm.nih.gov/pubmed/22330017
所以说重训的日子睡觉之前吃点dairy吧~
Med Sci Sports Exerc. 2012 Aug;44(8):1560-9. doi: 10.1249/MSS.
0b013e31824cc363.
Protein ingestion before sleep improves postexercise overnight recovery.
Res PT, Groen B, Pennings B, Beelen M, Wallis GA, Gijsen AP, Senden JM, VAN
Loon LJ.
Source
Department of Human Movement Sciences, NUTRIM School for Nutrition,
Toxicology and Metabolism, Maastricht University Medical Centre+, Maastricht
, The Netherlands.
Abstract
INTRO... 阅读全帖 |
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p*********l
发帖数: 26270 | 49 http://www.sparkpeople.com/resource/nutrition_articles.asp?id=5
Alcohol is metabolized differently than other foods and beverages. Under
normal conditions, your body gets its energy from the calories in
carbohydrates, fats and proteins, which are slowly digested and absorbed
within the gastrointestinal system. However, this digestive process changes
when alcohol is present. When you drink alcohol, it gets immediate attention
(because it is viewed by the body as a toxin) and needs no digestion.
O... 阅读全帖 |
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j*j
发帖数: 5564 | 50 原作者Yann Martel在写了《Life of Pi》不久之后，又写了一部作品，
《We ate the children last》
可见他对吃人这个话题耿耿于怀，难以摆脱。这暗示他写《Life of Pi》的真实意图
中确实有写人吃人那一层意思，这层意思起码占50%吧。
说白了，写小说就是一个游戏，他故意要写得亦真亦幻，让那些经历不同信仰不同的读
者们对此作不同的解读。
-------------------------------------------------
We ate the children last
Yann Martel
The Guardian, Friday 16 July 2004
The first human trial was on Patient D, a 56-year-old male, single and
childless, who was suffering from colon cancer. He was a skeletal man with
white, bloodless skin who could no longer ... 阅读全帖 |
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