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h*****4
发帖数: 1158 | 2 厉害
关键是找到了工作，
lz能否介绍一下你的具体方向和工业界的运用么？
（我知道不少做biofuel就是做些promoter engineering或是搞突变筛突变株，好像有
点boring呵呵，楼主不要介意哈） |
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b******k
发帖数: 1874 | 3 for this topic, many ppl replied. I have compiled some of them here. (btw, s
ome of them are better than the one marked). while majority of ppl suggest t
o add Pi tto oligo 3' end by TNK, I don't know why. Anyone could help give r
elated mechanisms? Thanks a lot.
???: smartkevin (Biofuels), ??: Biology
? ?: Re: ????:?????30bp?DNA fragment ???vector?
???: BBS ????? (Tue May 18 04:01:41 2010, ??)
??oligo?????????????,???????????
??????protocol,??????:
1. oligos?TE or H2O??,??100 uM;
2. ???????,37 |
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s******y
发帖数: 28562 | 4 我的那个朋友水平其实不错，但是他博士的时候是做植物的，后来找博士后的
时候有好几个offer包括一个做biofuel的大牛院士（我一直怂恿他去那个）
但是他觉得自己植物做的太久了继续作没有意思，于是一心要转个方向，
当时给他offer的大部分都是和植物相关的，只有一个用老鼠作model的刚刚
设立实验室的新PI那里也给了他offer。于是他就一心要去那个实验室。当时
我大惊，拼命劝说他不要去，因为我也做一些老鼠，知道这个领域的残酷性。
可是他不听，说对方说了能够发CNS. 于是竟去了。然后去了两年，据他说
适应起来非常艰难（这个我理解，老鼠可不是那么容易上手的）。更糟糕的是
他的老板主意极多而且变得极快，经常让他换项目，然后他起早摸黑的刻苦
干了两年，什么都没有做出来，虽然他老板人其实不坏，但还是黯然的退了
（因为感到绝望），幸好他博士时候发的文章足够好，让他在一个生物公司
里找到了一个位置。
friend
very |
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h**********8
发帖数: 650 | 5 It was posted by Novoenzyme.
Biofuel will be as prosperous as Solar energy. |
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r********7
发帖数: 229 | 6 biofuel and biomaterial?
找个基因突变了（或者就像袁隆平那样筛选优良品种--如果担心genetic
contamination的话），然后导致feedstock的bio-ethanol, bio-diesel
syngas, etc的产量大增。
虽然这块受政府扶持(subsidize等)很严重，不过现在中国不是提倡绿色，低
碳，减排吗。加上BP漏油的事件，估计这块会热。然后农民伯伯就有生意
了：）
都打赤膊
一户人家
我会尽量 |
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s*3
发帖数: 352 | 7 酿酒酵母的戊糖利用途径不是早就做出来了么？
印象中有若干文献，专利了；利用该途径用于biofuel也算不上“新”了吧？一堆人都
在做的。 |
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s**********e
发帖数: 2888 | 8 你说的这个，其实就学术career(就是混碗饭吃）来说，也是非常不好的一条道路，也许做biofuel的会好一点，我就不知道了。
如果是做natural product biosynthesis，非常的不好。因为学术界的需求很少，到现在为止还都是纯粹的理论研究。所以出来的faculty都是那么几个大牛的学生，基本上如果你不是那么几个大牛，比如Christopher Walsh的学生，你几乎不可能在美国学术界找到位置。你看看最新的这个领域的faculty，几乎都是walsh的学生，老人不算。
我觉得原因是因为，这块的研究其实是几块的混合，genetics, biochemistry,
chemistry。最后的结果就是，你得genetics没有genetics的做得好，真正做genetics的人觉得你做的是小儿科；真正做biochemistry/chemistry的人也觉得你做得很简单。你牛的地方是这几块你都会做。
所以，纯粹的理论价值有限，应用前景不明了。其实说穿了，就是想和synthetic chemistry竞争。也许在遥远的未来，microbial biosynthesis会超过s |
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f**u
发帖数: 346 | 9 但问题是有些东西你不用生物合成不行。著名的紫杉醇就是一个例子。
我个人觉得如果你确定了一个天然产物有应用价值，但是没法化学合成，
那么去找biosynthetic pathway应该是应用前景很大的工作，当然难度是另一码事。
也许做biofuel的会好一点，我就不知道了。
现在为止还都是纯粹的理论研究。所以出来的faculty都是那么几个大牛的学生，基本
上如果你不是那么几个大牛，比如Christopher Walsh的学生，你几乎不可能在美国学
术界找到位置。你看看最新的
genetics的人觉得你做的是小儿科；真正做biochemistry/chemistry的人也觉得你做得
很简单。你牛的地方是这几块你都会做。
chemistry竞争。也许在遥远的未来，microbial biosynthesis会超过synthetic
chemistry，但是现在来看，synthetic chemistry还是非常的成熟强大稳定。 |
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h**********r
发帖数: 671 | 10 在下做点biofuel相关的课题。两个月前从一个教授那儿要到了一株菌，其实也可以从
欧洲一个菌种保存机构买到。要回来之后，这期间构建了一些pathway进去，结果也可
以。但是最近发现原来给我菌的教授也做了相关的工作。但是文章没有发表，有一些媒
体报道。请问这种情况下怎么办？
1. 写email交流一下，写文章让其coauthor?
2. 停止手头上的东西，因为别人也可能submit了类似的paper?
3. 自己马上把实验补完，submit文章？
很纠结，别人能把菌给咱，真的是很nice。不想让别人觉得我太mean。
求建议，多谢！！！ |
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S****y
发帖数: 751 | 11 现在跟biofuel结合炒的很火，不知道这个方向就业前景怎么样 |
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k*****o
发帖数: 1486 | 12 It is interesting, but a long way to go...
Biofuel is a promising project, but to scale up the production is
difficult.
If you use algae, that's gonna take a lot of spaces, as you have seen from
the discovery channel. Mechanical and chemical engineers are trying to make
some space-saving "bucket" to make it work, but I don't think it is going to
be easy.
If you use E. coli, the efficient degradation process of a cheap resource, e
.g. fiber, is required. There has been no such a powerful enzyme y |
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z*h
发帖数: 773 | 13 Another point is that in academia, some persons are praised like God. In
fact, they are so-so. Given the same chance, you may do better than them.
At least, Keasling's work is not new nor important. His biofuels project
will go to a dead end surely. After 10 years, most will know it. |
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g*********r
发帖数: 9366 | 14 迄今为止所有的biofuel都不能赚钱， 而且在可预见的未来也不能赚钱
这个行业更多的是用来探索新技术，并且保持之，
这里面政治因素多于经济考虑 |
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z*h
发帖数: 773 | 17 That is right.
In biofuels, Nature and Science published so many trash papers recently.
Because they are member of the club. In fact, they do not understand
research topics at all. |
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d*****r
发帖数: 2583 | 18 http://www.the-scientist.com/news/display/57865/
And now for the next 50 years...
Check out what attendees of this year's ASCB meeting predict will occur
in the next 50 years of biology
2010-2019
Epigenetic cancer drugs
Identification of what "junk" DNA does
1 nm resolution light microscopy
High content screening
Digital displays for posters
Full human delivery of siRNA
2020-2029
HIV vaccine
Cure for ADD/ADHD
Bioartificial organ
Protein folding solved
First human clone
Nanocrystalline buildings ... 阅读全帖 |
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s********n
发帖数: 2939 | 19 我也不是老手啊，这个conference也是别人推荐的，感觉还不错，牛人很多，比如
frances arnold, roger tsein，他们的presentation都非常棒，虽然我对FA的看法有
些不是很赞同的。还有像Jens Neilsen更是metabolic engineering的大牛前辈，能够
目睹他们的风采还是很值得的。
看你的研究领域，个人觉得这个meeting更fundamental一些，有不少参加者都是做和
pharmaceutics相关的；如果是做biofuels和chemicals的话则可能参加像SIM，ASM比较
好。 |
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I*****h
发帖数: 470 | 21 "algae or corn"都是dead end for sure
industry |
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l*********1
发帖数: 351 | 24 corn是肯定的dead end。algae看不清楚 |
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O******e
发帖数: 4845 | 25 那篇论证Corn-Ethanol比石油对环境的破坏还大的Science paper出来后，生物燃料
立马就倒退了。 |
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I*****h
发帖数: 470 | 27 简单点
对于algae
一是效率
二是收集algae造的fuel消耗能源太大
成本，成本，还是成本
没看Shell都撤了algae了？ |
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W****C
发帖数: 1937 | 30 飞机除了石油大概只能用生物油了， 可是谁知道石油能用多少年啊？ 谁愿意把自己
的一辈子就去给别人做个备份？ |
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T*********I
发帖数: 10729 | 31 好多大的新闻媒体转载，比如ＡＢＣｎｅｗｓ．
Mass. company making diesel with sun, water, CO2
CAMBRIDGE, Mass. (AP) -- A Massachusetts biotechnology company says it can
produce the fuel that runs Jaguars and jet engines using the same
ingredients that make grass grow.
Joule Unlimited has invented a genetically-engineered organism that it says
simply secretes diesel fuel or ethanol wherever it finds sunlight, water and
carbon dioxide.
The Cambridge, Mass.-based company says it can manipulate the organism to
produce the... 阅读全帖 |
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H****s
发帖数: 301 | 32 Efficient recovery of biofuel from algae is a challenge everywhere. |
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I*****h
发帖数: 470 | 35 Lee Lynd
Jay Keasling
James Liao
随便去一个你就牛逼了！ |
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d***y
发帖数: 8536 | 36 Jay Keasling是最NB的，大牛中的战斗机
James Liao是个台湾人，不错貌似组里大陆人也很多。 主要搞butanol isobutanol的
，现在也是很NB的人，拿到过总统啥奖
Lee Lynd也是很牛的人物，组里的工作主要是围绕ethanol的。 |
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k*****o
发帖数: 1486 | 41 作Biofuel的有些大牛（不点名了），自己在做某organism，别人的就统统都没有前途
。听他们演讲回答问题就可以预料到，要是自己文章送他们那得结果。 |
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h**********r
发帖数: 671 | 42 http://stkarnick.com/culture/2011/03/21/national-science-founda
On the February 23rd broadcast, a synthetic biologist from Nebraska named
Jay Keasling was profiled. Keasling’s interests are currently focused on
synthetic biofuels research and, it’s made abundantly clear, having sex
with other men.
Jay Keasling
Keasling admits he’s gay, but because of peer pressure in the Bible-belt
Midwest he didn’t “come out” until he moved to Berkeley to do research.
His father, in a brief insert, says he acce... 阅读全帖 |
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h**********r
发帖数: 671 | 43 对的，算是搞biofuel的吧。唉，可惜真的伤不起啊～～～对了，这个会议有人去吧。
33rd Symposium on Biotechnology for Fuels and Chemicals
May 2-5, 2011
Sheraton Seattle
Seattle, WA
到时候好好向大家学习学习。如果版上那位老兄faculty了，跟着做做postdoc.好好过
过metabolic engineering/synthetic biology的瘾。 |
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s******9
发帖数: 283 | 44 呵呵，老板私底下对他研究有相似的评价。想当初联系他postdoc的时候email，mail都
石沉大海。现在一看见人说用bacteria合成pharmaceutical，biofuel就觉得boring。
scaffold |
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l*********1
发帖数: 351 | 45 不做pharma,不做biofuel,那做啥呢?如果能分享一下心得的话.做biological tools满
足好奇心? |
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z*h
发帖数: 773 | 46 The use of metabolic model for simulation of complicated biological system
is a day dream based on available knowledge. The father of metabolic
engineering -- JE Bailey -- pointed it out in 1998.
Bailey, J. E. (1998) Mathematical modeling and analysis in biochemical
engineering: past accomplishments and future opportunities, Biotechnol. Prog
. 14, 8-20.
Who are really hearing his suggestions? In vivo synthetic biology for
biofuels production will be a dead end surely because of poor efficiencies... 阅读全帖 |
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h**********r
发帖数: 671 | 47 http://stkarnick.com/culture/2011/03/21/national-science-founda
On the February 23rd broadcast, a synthetic biologist from Nebraska named
Jay Keasling was profiled. Keasling’s interests are currently focused on
synthetic biofuels research and, it’s made abundantly clear, having sex
with other men.
Jay Keasling
Keasling admits he’s gay, but because of peer pressure in the Bible-belt
Midwest he didn’t “come out” until he moved to Berkeley to do research.
His father, in a brief insert, says he acce... 阅读全帖 |
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h**********r
发帖数: 671 | 48 对的，算是搞biofuel的吧。唉，可惜真的伤不起啊～～～对了，这个会议有人去吧。
33rd Symposium on Biotechnology for Fuels and Chemicals
May 2-5, 2011
Sheraton Seattle
Seattle, WA
到时候好好向大家学习学习。如果版上那位老兄faculty了，跟着做做postdoc.好好过
过metabolic engineering/synthetic biology的瘾。 |
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s******9
发帖数: 283 | 49 呵呵，老板私底下对他研究有相似的评价。想当初联系他postdoc的时候email，mail都
石沉大海。现在一看见人说用bacteria合成pharmaceutical，biofuel就觉得boring。
scaffold |
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l*********1
发帖数: 351 | 50 不做pharma,不做biofuel,那做啥呢?如果能分享一下心得的话.做biological tools满
足好奇心? |
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