b*****n
发帖数: 1841 | 1 现在有两个实验室可能接纳我。一个实验室做老鼠模型的，刚建实验室，模型是我最喜
欢做的，可以学到很多新东西，以后的道路偏基础研究。一个实验室很有名，做小分子
筛选的，很有应用前景，可以申请专利往工业界发展，但是没啥新东西可以学，做的多
数是cell based的东西，不用太动脑筋，自己去纯劳动力。从发文章的角度来说，前者
更可能发好文章，本人比较偏向于喜欢做研究。
想听听大家的意见，如果是你，你会选择哪个实验室？ |
s******y
发帖数: 28562 | 2 刚建立的实验室最好不要去！除非你和对方很熟悉，知道对方有能力有人品
有充分资金。
要是我，当然选第二个，别把豆包不当干粮。很多以前习惯作基础研究的人
往往轻视做生物工程的人，觉得他们的技术含量不高，这个其实是一个隔行如
隔山的误会，因为不同行业的技术思路是不同的不能直接比较。
而且，生物工程实验室出来的人，除了可以到工业界，还很容易找法考题职位。
【在 b*****n 的大作中提到】
: 现在有两个实验室可能接纳我。一个实验室做老鼠模型的，刚建实验室，模型是我最喜
: 欢做的，可以学到很多新东西，以后的道路偏基础研究。一个实验室很有名，做小分子
: 筛选的，很有应用前景，可以申请专利往工业界发展，但是没啥新东西可以学，做的多
: 数是cell based的东西，不用太动脑筋，自己去纯劳动力。从发文章的角度来说，前者
: 更可能发好文章，本人比较偏向于喜欢做研究。
: 想听听大家的意见，如果是你，你会选择哪个实验室？
|
a***e
发帖数: 1010 | 3 生物工程方向的比基础研究在找 faculty 上和找工作上都好找多了.
basic science 的或者某些诡异的模式生物的，那真是惨啦。 |
b*****n
发帖数: 1841 | 4 小分子筛选我已经做过了，知道怎么回事，他之所以对我感兴趣，也是因为背景fit。
本人比较擅长想idea，比较抗拒做重复劳动。
“生物工程实验室出来的人，除了可以到工业界，还很容易找法考题职位”。我就怕做
了这个大规模筛选的东西以后不容易进学术界。可有什么例子？
【在 s******y 的大作中提到】
: 刚建立的实验室最好不要去！除非你和对方很熟悉，知道对方有能力有人品
: 有充分资金。
: 要是我，当然选第二个，别把豆包不当干粮。很多以前习惯作基础研究的人
: 往往轻视做生物工程的人，觉得他们的技术含量不高，这个其实是一个隔行如
: 隔山的误会，因为不同行业的技术思路是不同的不能直接比较。
: 而且，生物工程实验室出来的人，除了可以到工业界，还很容易找法考题职位。
|
s******y
发帖数: 28562 | 5 Just look at the faculty list of most bioengineering department.
I seriously suggest you NOT to go to the first lab, otherwise, most likely,
you will regret you decision after one year.
I have seen something similar to your case in one of my friend. When he
was choosing his postdoc position, he insisted to get into a new lab despite
opposite suggestions from me and other friends. After two years' struggle in
that lab, he finally regretted his decision.
【在 b*****n 的大作中提到】
: 小分子筛选我已经做过了，知道怎么回事，他之所以对我感兴趣，也是因为背景fit。
: 本人比较擅长想idea，比较抗拒做重复劳动。
: “生物工程实验室出来的人，除了可以到工业界，还很容易找法考题职位”。我就怕做
: 了这个大规模筛选的东西以后不容易进学术界。可有什么例子？
|
p*****m
发帖数: 7030 | 6 我来搭车问问你觉得cell based的小分子筛选有什么技术含量没？还是说基本都成系统
了换个assay谁都能做起来呢？
【在 b*****n 的大作中提到】
: 小分子筛选我已经做过了，知道怎么回事，他之所以对我感兴趣，也是因为背景fit。
: 本人比较擅长想idea，比较抗拒做重复劳动。
: “生物工程实验室出来的人，除了可以到工业界，还很容易找法考题职位”。我就怕做
: 了这个大规模筛选的东西以后不容易进学术界。可有什么例子？
|
p*****m
发帖数: 7030 | 7 这个也未必吧 我边上两个新AP的lab 其中一个老板的第一个postdoc已经拿到AP offer
了（老板还没tenure呢） 另外一个的第一个postdoc也开始上market了。这两个还都是
比较普通的AP 那种rising star型的还不在讨论范围
,
despite
in
【在 s******y 的大作中提到】
: Just look at the faculty list of most bioengineering department.
: I seriously suggest you NOT to go to the first lab, otherwise, most likely,
: you will regret you decision after one year.
: I have seen something similar to your case in one of my friend. When he
: was choosing his postdoc position, he insisted to get into a new lab despite
: opposite suggestions from me and other friends. After two years' struggle in
: that lab, he finally regretted his decision.
|
s******y
发帖数: 28562 | 8 That really, really depends. My current mentor is also a new AP and I am
actually very happy in her lab. However that is because I am very sure about
her personality and her academic ability because we are in the same field
and
I have heard a lot of 8g about who is nice and who is not.
In LZ's case, he obvisouly want to change field and may not be fimiliar
with what the new lab is doing. In such a case, he has a big risk.
offer
【在 p*****m 的大作中提到】
: 这个也未必吧 我边上两个新AP的lab 其中一个老板的第一个postdoc已经拿到AP offer
: 了（老板还没tenure呢） 另外一个的第一个postdoc也开始上market了。这两个还都是
: 比较普通的AP 那种rising star型的还不在讨论范围
:
: ,
: despite
: in
|
b*****n
发帖数: 1841 | 9 没啥技术含量，机械手也没那么聪明稳定，有很多很细节的trouble-shooting。
重要的是你的assay有重要意义，申请grant能通过审核。
【在 p*****m 的大作中提到】
: 我来搭车问问你觉得cell based的小分子筛选有什么技术含量没？还是说基本都成系统
: 了换个assay谁都能做起来呢？
|
p*****m
发帖数: 7030 | 10 谢谢啦 那你觉得从一个assay 到能scale-up的screen系统 一般得多少时间呢？换句话
说就是把assay定量搞好能上高通量外加数据分析blabla这些工作会需要很长时间么？
【在 b*****n 的大作中提到】
: 没啥技术含量，机械手也没那么聪明稳定，有很多很细节的trouble-shooting。
: 重要的是你的assay有重要意义，申请grant能通过审核。
|
|
|
p*****m
发帖数: 7030 | 11 这个自然 不过人品问题去找人打听就好呀 反正就算是established PI也得打听不是
about
【在 s******y 的大作中提到】
: That really, really depends. My current mentor is also a new AP and I am
: actually very happy in her lab. However that is because I am very sure about
: her personality and her academic ability because we are in the same field
: and
: I have heard a lot of 8g about who is nice and who is not.
: In LZ's case, he obvisouly want to change field and may not be fimiliar
: with what the new lab is doing. In such a case, he has a big risk.
:
: offer
|
b*****n
发帖数: 1841 | 12 assay development，可行性数据收集，一般半年之内。grant 审批半年以上。
screening本身其实很快，机器一两个星期就做完了。数据分析很routine，也很快。
很多时间你要考虑develop counter screening，把你不要的compound排除掉， 否则一
般 cell based 的hits太多。
screening 本身是为后来铺路的，不能发什么好文章。
【在 p*****m 的大作中提到】
: 谢谢啦 那你觉得从一个assay 到能scale-up的screen系统 一般得多少时间呢？换句话
: 说就是把assay定量搞好能上高通量外加数据分析blabla这些工作会需要很长时间么？
|
p*****m
发帖数: 7030 | 13 I see 看来你们通常做法是拿到assay就申funding然后做HTS咯？是那种R21性质的然后
去NIH的平台作么？
【在 b*****n 的大作中提到】
: assay development，可行性数据收集，一般半年之内。grant 审批半年以上。
: screening本身其实很快，机器一两个星期就做完了。数据分析很routine，也很快。
: 很多时间你要考虑develop counter screening，把你不要的compound排除掉， 否则一
: 般 cell based 的hits太多。
: screening 本身是为后来铺路的，不能发什么好文章。
|
s******y
发帖数: 28562 | 14 I myself actually is planning to get into this field later this year...
【在 b*****n 的大作中提到】
: assay development，可行性数据收集，一般半年之内。grant 审批半年以上。
: screening本身其实很快，机器一两个星期就做完了。数据分析很routine，也很快。
: 很多时间你要考虑develop counter screening，把你不要的compound排除掉， 否则一
: 般 cell based 的hits太多。
: screening 本身是为后来铺路的，不能发什么好文章。
|
b*****n
发帖数: 1841 | 15 说实话，我现在不太在乎老板有多tough，只要reasonable，不同风格的老板我应该都
能handle。我觉得文章才是硬道理。否则爽几年，后面更悲惨。
about
【在 s******y 的大作中提到】
: That really, really depends. My current mentor is also a new AP and I am
: actually very happy in her lab. However that is because I am very sure about
: her personality and her academic ability because we are in the same field
: and
: I have heard a lot of 8g about who is nice and who is not.
: In LZ's case, he obvisouly want to change field and may not be fimiliar
: with what the new lab is doing. In such a case, he has a big risk.
:
: offer
|
p*****m
发帖数: 7030 | 16 我其实也是差不多思路 reasonable+supportive就够了
【在 b*****n 的大作中提到】
: 说实话，我现在不太在乎老板有多tough，只要reasonable，不同风格的老板我应该都
: 能handle。我觉得文章才是硬道理。否则爽几年，后面更悲惨。
:
: about
|
X******n
发帖数: 914 | 17 Sunnyday几时招postdoc说一声，我都等了很久了。 |
b*****n
发帖数: 1841 | 18 是啊！
assay development本身有一个R03可以申请。
【在 p*****m 的大作中提到】
: I see 看来你们通常做法是拿到assay就申funding然后做HTS咯？是那种R21性质的然后
: 去NIH的平台作么？
|
s******y
发帖数: 28562 | 19 There is a big risk to join new lab in the sense of publication.
If the lab has been set up for more than one year, then it is probably
alright because all the system should be working now. Otherwise, you
will bump into a lot of trouble. That is what happened to the friend I
mentioned. His postdoc boss has some nice publications beofore but
unfortunately has no clue to conduct research independently and
he wasted two years in his lab doing a lot of blind research that end up
no publications at a
【在 b*****n 的大作中提到】
: 说实话，我现在不太在乎老板有多tough，只要reasonable，不同风格的老板我应该都
: 能handle。我觉得文章才是硬道理。否则爽几年，后面更悲惨。
:
: about
|
s******y
发帖数: 28562 | 20 Ft...If you are waiting for me, then you will have to keep waiting...
【在 X******n 的大作中提到】
: Sunnyday几时招postdoc说一声，我都等了很久了。
|
|
|
p*****m
发帖数: 7030 | 21 3x~
【在 b*****n 的大作中提到】
: 是啊！
: assay development本身有一个R03可以申请。
|
X******n
发帖数: 914 | 22 啊，可惜可惜,我实在等不住了。
如果让你做小分子化合物筛选，你会选择哪个领域：autophagy，UPR，cancer，aging
，circadian clock... |
a***e
发帖数: 1010 | 23 不许跟我抢，
【在 X******n 的大作中提到】
: Sunnyday几时招postdoc说一声，我都等了很久了。
|
T**********t
发帖数: 1604 | 24 找博后跟买股票似的，能买得起绩优股当然好，买不起就只好押宝在潜力股身上了。。。
【在 s******y 的大作中提到】
: There is a big risk to join new lab in the sense of publication.
: If the lab has been set up for more than one year, then it is probably
: alright because all the system should be working now. Otherwise, you
: will bump into a lot of trouble. That is what happened to the friend I
: mentioned. His postdoc boss has some nice publications beofore but
: unfortunately has no clue to conduct research independently and
: he wasted two years in his lab doing a lot of blind research that end up
: no publications at a
|
X******n
发帖数: 914 | 25 我已经被据了！
【在 a***e 的大作中提到】
: 不许跟我抢，
|
j**********e
发帖数: 2 | 26
不同意这个说法，screening本身其实很快，机器一两个星期就做完了。我做全基因组
的siRNA筛选，仅仅筛选就做了2-3个月。依据过程的复杂程度和LIBRARY的大小以及重
复次数而定。
【在 b*****n 的大作中提到】
: assay development，可行性数据收集，一般半年之内。grant 审批半年以上。
: screening本身其实很快，机器一两个星期就做完了。数据分析很routine，也很快。
: 很多时间你要考虑develop counter screening，把你不要的compound排除掉， 否则一
: 般 cell based 的hits太多。
: screening 本身是为后来铺路的，不能发什么好文章。
|
p*****m
发帖数: 7030 | 27 人家是做small molecule screen，又不是RNAi screen
【在 j**********e 的大作中提到】
:
: 不同意这个说法，screening本身其实很快，机器一两个星期就做完了。我做全基因组
: 的siRNA筛选，仅仅筛选就做了2-3个月。依据过程的复杂程度和LIBRARY的大小以及重
: 复次数而定。
|
h********n
发帖数: 4079 | 28 我不能说你高估了自己, 但有可能你低估了老板变态的可能性和强度.
【在 b*****n 的大作中提到】
: 说实话，我现在不太在乎老板有多tough，只要reasonable，不同风格的老板我应该都
: 能handle。我觉得文章才是硬道理。否则爽几年，后面更悲惨。
:
: about
|
c*******y
发帖数: 1657 | 29 前者太冒险
选后者
【在 b*****n 的大作中提到】
: 现在有两个实验室可能接纳我。一个实验室做老鼠模型的，刚建实验室，模型是我最喜
: 欢做的，可以学到很多新东西，以后的道路偏基础研究。一个实验室很有名，做小分子
: 筛选的，很有应用前景，可以申请专利往工业界发展，但是没啥新东西可以学，做的多
: 数是cell based的东西，不用太动脑筋，自己去纯劳动力。从发文章的角度来说，前者
: 更可能发好文章，本人比较偏向于喜欢做研究。
: 想听听大家的意见，如果是你，你会选择哪个实验室？
|
b*****n
发帖数: 1841 | 30 到实验室面试的时候基本可以确定人会变态到什么程度。我不太相信自己走眼太厉害。
要是真走眼的，那到时候换实验室吧。
【在 h********n 的大作中提到】
: 我不能说你高估了自己, 但有可能你低估了老板变态的可能性和强度.
|
|
|
s******y
发帖数: 28562 | 31 I think I will actually do virus :) I am always interested in that area but
have never really done anything in it.
aging
【在 X******n 的大作中提到】
: 啊，可惜可惜,我实在等不住了。
: 如果让你做小分子化合物筛选，你会选择哪个领域：autophagy，UPR，cancer，aging
: ，circadian clock...
|
n********k
发帖数: 2818 | 32 well, publication wise, it really depends on the research directions,
project and whether he/she has vision or
not, and how independent she/he can be...And no offense, it seems ur friend
wasn't good enough unless
his/her boss is stupid and arrogant...But I have to agree it is tough/very
challenging to join a new lab...I
would think it over and over before I would choose a junior lab over a
reputable senior lab...one more point if
I might disagree: I would join a lab which is brand new with no
【在 s******y 的大作中提到】
: There is a big risk to join new lab in the sense of publication.
: If the lab has been set up for more than one year, then it is probably
: alright because all the system should be working now. Otherwise, you
: will bump into a lot of trouble. That is what happened to the friend I
: mentioned. His postdoc boss has some nice publications beofore but
: unfortunately has no clue to conduct research independently and
: he wasted two years in his lab doing a lot of blind research that end up
: no publications at a
|
t****p
发帖数: 1504 | 33 Good point.
friend
very
【在 n********k 的大作中提到】
: well, publication wise, it really depends on the research directions,
: project and whether he/she has vision or
: not, and how independent she/he can be...And no offense, it seems ur friend
: wasn't good enough unless
: his/her boss is stupid and arrogant...But I have to agree it is tough/very
: challenging to join a new lab...I
: would think it over and over before I would choose a junior lab over a
: reputable senior lab...one more point if
: I might disagree: I would join a lab which is brand new with no
|
s******y
发帖数: 28562 | 34 我的那个朋友水平其实不错，但是他博士的时候是做植物的，后来找博士后的
时候有好几个offer包括一个做biofuel的大牛院士（我一直怂恿他去那个）
但是他觉得自己植物做的太久了继续作没有意思，于是一心要转个方向，
当时给他offer的大部分都是和植物相关的，只有一个用老鼠作model的刚刚
设立实验室的新PI那里也给了他offer。于是他就一心要去那个实验室。当时
我大惊，拼命劝说他不要去，因为我也做一些老鼠，知道这个领域的残酷性。
可是他不听，说对方说了能够发CNS. 于是竟去了。然后去了两年，据他说
适应起来非常艰难（这个我理解，老鼠可不是那么容易上手的）。更糟糕的是
他的老板主意极多而且变得极快，经常让他换项目，然后他起早摸黑的刻苦
干了两年，什么都没有做出来，虽然他老板人其实不坏，但还是黯然的退了
（因为感到绝望），幸好他博士时候发的文章足够好，让他在一个生物公司
里找到了一个位置。
friend
very
【在 n********k 的大作中提到】
: well, publication wise, it really depends on the research directions,
: project and whether he/she has vision or
: not, and how independent she/he can be...And no offense, it seems ur friend
: wasn't good enough unless
: his/her boss is stupid and arrogant...But I have to agree it is tough/very
: challenging to join a new lab...I
: would think it over and over before I would choose a junior lab over a
: reputable senior lab...one more point if
: I might disagree: I would join a lab which is brand new with no
|
O******e
发帖数: 4845 | 35 The 1st PI might be a rising star.....
,
despite
in
【在 s******y 的大作中提到】
: Just look at the faculty list of most bioengineering department.
: I seriously suggest you NOT to go to the first lab, otherwise, most likely,
: you will regret you decision after one year.
: I have seen something similar to your case in one of my friend. When he
: was choosing his postdoc position, he insisted to get into a new lab despite
: opposite suggestions from me and other friends. After two years' struggle in
: that lab, he finally regretted his decision.
|
O******e
发帖数: 4845 | 36 说技术含量低，估计他也是没深入做下去。
【在 p*****m 的大作中提到】
: 我来搭车问问你觉得cell based的小分子筛选有什么技术含量没？还是说基本都成系统
: 了换个assay谁都能做起来呢？
|
b******d
发帖数: 149 | 37 I think that you guys misunderstand small molecule screening. The design of
assay is very tricky and state of the art. Once you get interesting hits,
identifying the target and studying biological mechanism is even more
interesting. This often leads to unexpected, yet big discovery. There have
been some break through in target identification methods (find it in your
favorite search engine) and big discovery in Nature (and its sister journal)
and Cells. For example, today's CELL paper by Ste |
b******d
发帖数: 149 | 38 I think that you guys misunderstand small molecule screening. The design of
assay is very tricky and state of the art. Once you get interesting hits,
identifying the target and studying biological mechanism is even more
interesting. This often leads to unexpected, yet big discovery. There have
been some break through in target identification methods (find it in your
favorite search engine) and big discovery in Nature (and its sister journal)
and Cells. For example, today's CELL paper by Ste |
p*****m
发帖数: 7030 | 39 hoho 话说31902我才刚刚提过这篇Paper 不过这文章里assay design没什么特别之处吧
比较惊人的就是他们居然真的做了这个screen
of
,
have
journal)
【在 b******d 的大作中提到】
: I think that you guys misunderstand small molecule screening. The design of
: assay is very tricky and state of the art. Once you get interesting hits,
: identifying the target and studying biological mechanism is even more
: interesting. This often leads to unexpected, yet big discovery. There have
: been some break through in target identification methods (find it in your
: favorite search engine) and big discovery in Nature (and its sister journal)
: and Cells. For example, today's CELL paper by Ste
|
X******n
发帖数: 914 | 40 1000个化合物，我觉得可以接受了。听了那个采访，这个项目大概做了5年，筛选用了2
年时间。但是可惜的是，还是没有找到target，说实在的，这才是最有趣其实也是最难
的部分。 |
|
|
s******y
发帖数: 28562 | 41 I am very surpsied that their protocol can pass the approval.
Isn't against the rule to do random screening on animal?
【在 p*****m 的大作中提到】
: hoho 话说31902我才刚刚提过这篇Paper 不过这文章里assay design没什么特别之处吧
: 比较惊人的就是他们居然真的做了这个screen
:
: of
: ,
: have
: journal)
|
p*****m
发帖数: 7030 | 42 我觉得target不是问题吧 要说现在还有n多的FDA approved drug没有已知target
了2
【在 X******n 的大作中提到】
: 1000个化合物，我觉得可以接受了。听了那个采访，这个项目大概做了5年，筛选用了2
: 年时间。但是可惜的是，还是没有找到target，说实在的，这才是最有趣其实也是最难
: 的部分。
|
p*****m
发帖数: 7030 | 43 真有这样的规定啊?
【在 s******y 的大作中提到】
: I am very surpsied that their protocol can pass the approval.
: Isn't against the rule to do random screening on animal?
|
T**********t
发帖数: 1604 | 44 PETA will be PITA for those people.
【在 p*****m 的大作中提到】
: 真有这样的规定啊?
|
b******d
发帖数: 149 | 45 Target and mechanism are very important to understand the basic biological
problem as well as how potential drug works and what the side effects are.
A good small molecule hit with targets and insights often opens up a new
field or advances a field that has no progress for ages (speaking of which,
check today's Nature Biotechnology!). It also speeds up drug design,
development and avoid all potential side effects and law suits. How many
lawsuits and bad PR of existing FDA approved drugs have |
X******n
发帖数: 914 | 46 Good point。
看了那两篇文章，rapamycin是我最喜欢的化合物之一。没想到Huang’lab做了这么好
的工作， 不亏是Shreiber的弟子。早听说Mike筛选了无数化合物，手上有上千化合物
有好的phenotype，看来到出东西的时候了。酵母这个模型做筛选还是有优势。
,
【在 b******d 的大作中提到】
: Target and mechanism are very important to understand the basic biological
: problem as well as how potential drug works and what the side effects are.
: A good small molecule hit with targets and insights often opens up a new
: field or advances a field that has no progress for ages (speaking of which,
: check today's Nature Biotechnology!). It also speeds up drug design,
: development and avoid all potential side effects and law suits. How many
: lawsuits and bad PR of existing FDA approved drugs have
|
