z*h
发帖数: 773 | 1 The use of metabolic model for simulation of complicated biological system
is a day dream based on available knowledge. The father of metabolic
engineering -- JE Bailey -- pointed it out in 1998.
Bailey, J. E. (1998) Mathematical modeling and analysis in biochemical
engineering: past accomplishments and future opportunities, Biotechnol. Prog
. 14, 8-20.
Who are really hearing his suggestions? In vivo synthetic biology for
biofuels production will be a dead end surely because of poor efficiencies... 阅读全帖 |
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h**********r
发帖数: 671 | 2 paper1
Title: optimization of a blueprint for in vitro glycolysis by metabolic real
-time analysis
Nature Chemical Biology (2011) DOI: doi:10.1038/nchembio.541
Matthias Bujara,1 Michael Schümperli,2 René Pellaux,1, 2 Matthias
Heinemann2, 3 & Sven Panke1, 2
Paper2
Title: Enzyme mechanism as a kinetic control element for designing synthetic
biofuel pathways
Brooks B Bond-Watts,1, 2 Robert J Bellerose1, 2 & Michelle C Y Chang1, 2, 3
Nature Chemical Biology, Volume: 7, Pages: 222–227 (2011)
Please s... 阅读全帖 |
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a********e
发帖数: 1181 | 3 Mark Rank Abbreviated Journal Title
(linked to journal information) ISSN JCR Data More Information
EigenfactorTM MetricsMore Information
Total Cites Impact
Factor 5-Year
Impact
Factor Immediacy
Index Articles Cited
Half-life EigenfactorTM
Score Article InfluenceTM
Score
1 NAT BIOTECHNOL 1087-0156 34520 31.085 29.061 5
.342 117 6.2 0.13226 12.257
2 NAT REV DRUG DISCOV 1474-1776 14742 28... 阅读全帖 |
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a********k
发帖数: 2273 | 4 JCR Year and Edition: 2010 Science
Abbreviated Journal Title ISSN {2010} Total Cites Impact Factor
5-Year Impact Factor Immediacy Index {2010} Articles Cited Half-
Life Eigenfactor Score Article Influence Score
CA-CANCER J CLIN 0007-9235 9801 94.262 70.216 8.667 18
3.8 0.04923 24.782
ACTA CRYSTALLOGR A 0108-7673 13944 54.333 24.717 0.629 70
6.2 0.04... 阅读全帖 |
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z*h
发帖数: 773 | 5 Good summary. In our field (biofuels), most of science and nature papers
are like it. |
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p*******r
发帖数: 4048 | 6 Interesting, can you pleae provide a link to the original article? |
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d***y
发帖数: 8536 | 8 吹牛也是要本事的。用这个方法产的biofuel,价值超过了钻石，是一种崭新的方法。 |
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s****r
发帖数: 736 | 9 小熊，就当这是一份salad。原料和组合都是旧的，就是份量较普通的多了点。即便如
此，原料和酱的处理都有不可取的地方。更关键的是，这salad物廉价高。按这种法门
做下去，biofuels这个行业破产就是早晚。 |
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z*h
发帖数: 773 | 10 Most biofuels seemingly "big" papers just cheated laymen.
Example 1. E.J. Steen, Y. Kang, G. Bokinsky, Z. Hu, A. Schirmer, A. McClure,
S.B. del Cardayre, J.D. Keasling, Microbial production of fatty-acid-
derived fuels and chemicals from plant biomass, Nature, 463 (2010) 559-562.
This is a combination of X. Lu, H. Vora, C. Khosla, Overproduction of free
fatty acids in E. coli: Implications for biodiesel production, Metab. Eng.,
10 (2008) 333-339. and a lot of old papers about xylan-utilization.
... 阅读全帖 |
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l*********1
发帖数: 351 | 11 K biofuels方面的工作被您批了很多次了吧?为啥大家都买他帐呢?他那个artemisinic
acid的工作当初吹得那么神乎奇迹最后还是啥都没有发生...
请问作代谢工程的到底哪个方向才是真正赢利,站得住脚的?
McClure,
,
Petzold
753 |
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z*h
发帖数: 773 | 12 Biofuels R&D is not basic science. You must compete with available systems
and fuels. Keep it in mind, we have enough fossil fuels that can last
another several hundred years. (Check more data, you will know that you are
misled.)
Keasling's work does not equal invention of locomotive. His work may have
great rooms for further improvement. But even his best future cannot compete
with available systems or other systems. It is why his work does not make
sense now or in the future. |
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x********u
发帖数: 430 | 13 To Prof. Zhang,
I think you have pointed out the truth. Your paper should go to Current
Opinions in Biotechnology not just Process Biochemistry. I went to the
Electrofuels conference at Providence last month, people in the conference are
talking a lot about biohydrogen and artificial photosynthesis. Non-
cellulosic sugar substrate is a dead end .By the way, Kealsing's fatty acids
project is not the end. One guy (Steven Singer) from JBEI is still working
on this project. Their next step is to eng... 阅读全帖 |
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z*h
发帖数: 773 | 14 Biofuels production from biomass would be sufficient to replace most
applications of crude oil. The keys are to increase energy efficiency and
decrease costs. |
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L*******r
发帖数: 5448 | 15 Environment Sci&Tech刚有篇文章说把biomass变成biofuel来减排的效率远不如
直接用biomass烧火取暖 |
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s********n
发帖数: 2939 | 16 Zyh讨论的其实很多人都看到了，但biofuel还是这么热，其实其背后有大量不同的政治
力量在博弈，说到底和美国的政治关系密切，并不是单纯的科研问题了。
还有就是化工产品对于石油总消耗而言只是小头，大概在5-10%，而能源要占到90-95%
，还有就是煤和天然气也是化石能源。 |
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s********n
发帖数: 2939 | 17 这种完全靠发酵菌株自身产的enzymes来水解biomass，也就是所谓的CBP，现阶段是完
全不现实的。比较现实的做法是用这种菌株（大部分是yeast表达fungal hydrolases）
联合fungi产的cellulases和hemicellulases来水解biomass，理论是可以减少enzyme
loading。
象JK的方向的确不为做工程的人所看重，所有生产biofuel过程的最大瓶颈就是cheap
feedstock，可以是sugars或者是“CO2+light”，后者看似免费，真正利用起来比
biomass还难。 |
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s********n
发帖数: 2939 | 18 根据DoE的能源报告，全世界生物质的产生量是全球能源消耗的10倍，可用来产生能源
而且不与食物冲突的生物质（主要是lignocellulose）大概与全球能源消耗量持平，当
然不同的model得到的结果有出入。
对于高价值的化工产品，对原材料的价格并不sensitive，用一般的glucose或者是生物
质产生的sugar并不是关键；但是对于biofuel而言cheap sugar是关键，只有利用所有
生物质产生的sugar甚至lignin monomers才有可能实现。 |
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f****e
发帖数: 679 | 21 paper sent to your gmail box |
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M**a
发帖数: 4816 | 22 Appreciated very much!
Baozi is coming!! |
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s*******s
发帖数: 623 | 24 各位有没有做工业微生物的
知不知道具体的regulation of genetically modified organism (GMO)？
具体一些：
比如用GMO生产biofuel或者biochemical，GMO并不会被释放到环境中去（因为发酵完毕
后要灭活的）
还需要通过regulation of GMO的审批不？
谢谢！ |
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x********u
发帖数: 430 | 25 It's just a 2-page commentary paper, not a perspective paper. His another
high-impact paper is a review paper about biofuels published on Annual
Reviews of Genetics.
The only research paper he had is one published on Metabolic engineering,
which is the extension of Xuefeng LV's work. By the way, it's ridiculous
that PNAS published their work about reconstitution of fatty acids synthase
multienzyme complex -- all about enzyme kinetics and protein purification.
The primary author for this Pnas pap... 阅读全帖 |
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b******s
发帖数: 1089 | 26 我们是植物领域做基础发育的。funding形式看起来很惨淡。老板只有一个NSF funding
，今年要到期，没有renew成功。实验室另外一个日本薄厚暑假之后必须要走人。老板
许诺我仍然会用startup的funding资助我。我对老板能否资助我倒不是很担心，但是对
自己未来的发展还是挺担心的。
据老板说，USDA现在基本只资助做crop相关的，DOE只资助photosynthesis和biofuel，
而NSF一个PI不能有超过一个grant的（除了genome research）。所以现在唯一的希望
是NIH，但是我们植物发育跟NIH联系起来非常困难。
我对这样的funding形式还是很担心的。我想再等几篇文章出来就上market，但是现在
看起来要么换方向，要么回国。否则在美国有可能很难拿到grant。换方向我也一直在
考虑，不过除非再去做一个薄厚，否则换起来会是一个漫长的过程。
版上有没有了解植物领域funding情况的给讲解讲解。一是上面funding的情形是否接近
事实？二是NIH对于植物领域的支持现在集中在哪些方向？三是，做植物的是不是剩下
回国一条路？最后，靠自己转方... 阅读全帖 |
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V**3
发帖数: 12756 | 27 生物信息北京，转到生物能源跨度很大吧
你原来是计算机背景，要转进到biofuel /metabolic engineering领域，困难不是一般
的大
（我没有理解错吧？）其中的分子生物学，和化学背景你有么？
而且你最好想清楚是否想做这个领域
这个领域恐怕是下一个被戳破的骗局
迄今为止，和可预见的将来，这个东西都是不能赚钱的，都是只能靠政治支撑 |
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w*********r
发帖数: 3 | 28 A postdoc and a technician position are available in Prof. Zhang's lab at
the Washington University in St. Louis starting from Fall 2012 (Chemical
Engineering department).
For the postdoc position, his/her project will focus on design of synthetic
biology tools for the microbial production of biofuels and pharmaceuticals.
Candidates should have background in Metabolic Engineering, Synthetic
Biology, or experience working with cyanobacteria.
For the technician position, solid training in molecula... 阅读全帖 |
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l**********1
发帖数: 5204 | 30 Re LX request: 能不能给列出几个这样做的组?
Here you go
http://www.csh-asia.org/design11.html
Keynote Speakers:
Jay Keasling, University of California, Berkeley, USA
Invited Speakers:
Adam Arkin, University of California, Berkeley, USA
Yaakov (Kobi) Benenson, ETH Zurich, SWITZERLAND
Austin Burt, Imperial College London, UK
Jeff Boeke, Johns Hopkins University, USA
Michelle Chang, University of California, Berkeley, USA
Virginia Cornish, Columbia University, USA
John Dueber, University of California, Berkeley... 阅读全帖 |
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l**********1
发帖数: 5204 | 31 Re LX request: 能不能给列出几个这样做的组?
Here you go
http://www.csh-asia.org/design11.html
Keynote Speakers:
Jay Keasling, University of California, Berkeley, USA
Invited Speakers:
Adam Arkin, University of California, Berkeley, USA
Yaakov (Kobi) Benenson, ETH Zurich, SWITZERLAND
Austin Burt, Imperial College London, UK
Jeff Boeke, Johns Hopkins University, USA
Michelle Chang, University of California, Berkeley, USA
Virginia Cornish, Columbia University, USA
John Dueber, University of California, Berkeley... 阅读全帖 |
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l*******6
发帖数: 128 | 32 非常感谢Ruyang 和 bestia的详细回复，其中提到了很多重要的POINT，受益匪浅！我
第一次面试是网上视频，做了30-40分钟PRESENTATION,中间他们也提问，然后和这家小
公司的员工和CEO都分别谈了一下。最后CEO以幻灯片的形式介绍了一下公司情况及技术
，我也就此问了些技术层面的问题。CEO说还有几个CANDIDATE，让我等消息，一周后通
知我ON-SITE面试。
当时的PRESENTATION他们反映不错，正如Ruyang 和 bestia所说的，有足够的细节，但
又不能够全部是细节，我把2个有些相关的PROJECT用一个大的TITLE和主线串起来，最
后结尾的时候给出个大的轮廓PICTURE，我觉得应该需要展示自己对本领域全局的了解
和把握程度，让他们可以认可自己的研究水平,觉得有点像申请PI的PRESENTATION.
断断续续3个月内申请了有几十个POSTED POSITION，公司的只有这一家邀请面试，其他
都没有消息。我是做微生物遗传的，觉得PUBLICATION 还可以（一作SCIENCE一篇,GENE
& DEV一篇,加上其他乱七八糟的约20篇）... 阅读全帖 |
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A********o
发帖数: 1307 | 33 Deng X-D, Gu B, Li Y-J, Hu X-W, Guo J-C, Fei X-W (2012) The Roles of acyl-
CoA: Diacylglycerol Acyltransferase 2 Genes in the Biosynthesis of
Triacylglycerols by the Green Algae Chlamydomonas reinhardtii. Mol Plant 5:
945-947
Huerlimann R, Heimann K (2012) Comprehensive guide to acetyl-carboxylases in
algae. Critical Reviews in Biotechnology Ahead of Print: 1-17
Jansson C (2012) Metabolic Engineering of Cyanobacteria for Direct
Conversion of CO2 to Hydrocarbon Biofuels. Progress in Botany: Progr... 阅读全帖 |
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a**y
发帖数: 464 | 34 当然不是每个学生都应该去盯着学术界的位置。特别是能力和背景本身就不强的
现在技术日新越易，工业界工作也不少。生物药物开发，next generation
sequencing，biofuel，biomaterial，其实眼光放开了，工作其实不少。 |
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y******8
发帖数: 1764 | 35 Abbreviated Journal Title ISSN {2012} Total Cites Impact Factor
5-Year Impact Factor Immediacy Index {2012} Articles Cited Half-
Life Eigenfactor Score Article Influence Score
CA-CANCER J CLIN 0007-9235 13722 153.459 88.55 27.04 25
3.3 0.0517 29.478
NEW ENGL J MED 0028-4793 245605 51.658 50.807 12.667 360
8 0.65776 21.494
REV MOD PHYS 0034-6861 35720 44.982 51.882 6.478 46 10
0.13048 32.634... 阅读全帖 |
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h**********r
发帖数: 671 | 36 An Engineered Microbial Platform for Direct Biofuel Production from Brown
Macroalgae
老早版上还讨论过这个日本postdoc和他的startup |
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c****a
发帖数: 2339 | 37 准备申请绿卡，若有以下方向审稿机会，非常感谢推荐:
email: c**********[email protected]
或站内信，多谢！
关键词: 生物能源 bioenergy
生物燃料 biofuel
环境微生物 environmental biology
生物催化 biocatalysis
酶工程，发酵工程 enzyme engineering,fermentation engineering
生物化工 biochemical engineering
生物反应器 bioreactor
食品科学 food science |
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j****n
发帖数: 3370 | 38 Metabolic engineering没你说的这么不堪吧 至少Dupont Dow都有很大的比例在做这个
，工业化的例子也不少了 bp shell等石油公司的biofuel department基本也是做
metabolic engineering的吧
★ 发自iPhone App: ChineseWeb 8.6 |
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h**********r
发帖数: 671 | 41 proposal的equipment这块儿需要一个fermentor的大致信息，包括大致价格和型号等。
大家有最近买发酵罐，或者quote过厂家的或者是从事这个行业的吗？要得比较急，来
不及online去quote.
We are working on yeast and fungi for biofuel production, which is an
aerobic process. The temperature can be 25-35C (usually around 30C). We
need to control pH, temperature and oxygen content. Both parallel and single
-Use bioreactors are OK for us. The budget is about $50,000. It can be 1 or
2-liter scale.
谢谢大家！ |
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g****d
发帖数: 238 | 42 不是新英格兰地区吧，宾州州大地处山中，老板算化学工程但实验主要微生物方面很杂
，生物膜，petsister cell， green chemistry，biofuel，toxin/antitoxin，
engineering Ab, 工业界有联系好多基金出自公司和能源部，但他希望找去学术界的人
做这persister cell机制，实验室以前博后一个同胞回国一个去别国教职，好像没看到
留美教职或去公司所以不清楚对以后出路有什么支持，q其实ABC的领域与我博士所学（
MDR transporter）都不同，去任何地方都不免学习新技术新背景，我对三个方向都有
兴趣，只想尽量出点好文章搞定身份以后学术还是业界看到时哪种可能实在不行回去 |
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m******g
发帖数: 467 | 43 Science 14.10.03
小知识：
1. The littlest patient
Cutting-edge mouse models fuel hope for understanding and treating cancer
2. How large predators manage the cost of hunting
3. How to survive being hot and inebriated
Biofuel, yeast |
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l********e
发帖数: 415 | 44 Sandia National Laboratories has an immediate and exciting opportunity for a
Post- Doctoral Appointee in Livermore, CA.
Candidates may contact Cathy Scott anytime for further information: cascot@
sandia.gov
___________________________________________________________________________
_______________________________________________________
Job Posting for Sandia National Laboratories in Livermore, CA. We are
seeking a Post doc in Virology, Immunology, Microbiology, Bioengineering or
relevant Ph... 阅读全帖 |
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s*******s
发帖数: 623 | 45 前几年发文章很牛的领域内的牛牛们不知有没有新进展？
像Keasling, James Liao等等 |
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c********n
发帖数: 225 | 46 【记录历史】谁重复验证了NgAgo？
项目 结题
date： 2017年8月2日
updated: 2017年8月4日
Note2017080401: added a few notable reviews/summaries
---------------------------------------
- NBT retraction note：
http://www.nature.com/nbt/journal/v34/n7/full/nbt.3547.html#correction2
原文引用
“Retracted online 02 August 2017
We are retracting our study because of the continued inability of the
research community to replicate the key results in Figure 4, using the
protocols provided in our paper. In this figure we report that the
Natro... 阅读全帖 |
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z*h
发帖数: 773 | 47 Dupont is cutting a lot of directions and selling them out.
They are focusing on selected directions. For example, emerging biofuels and
biobased materials. |
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m*****n
发帖数: 5245 | 48
&
topic
貌似Biofuel现在很hot |
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w****b
发帖数: 229 | 49 版主: Synthon karni 投票中，按V进入 讨论区 [
ChemicalEngineering]
离开[←,e] 选择[↑,↓] 阅读[→,r] 发表文章[Ctrl-P] 砍信[d] 备忘录[TAB] 求助[
h]
编号 刊 登 者 日 期 文章标题 在线/最高在线: 6/16 [一般模
式]
981 B ecochem Sep 6 ● New Faculty Position Available at Texas A&M
982 * handldk Sep 6 Re: 请教一下，大家觉得biofuels方向怎样？
983 * atomsgreat Sep 6 ● 恭喜板斧上任
984 * goddy Sep 6 Re: 恭喜板斧上任
985 * salt Sep 6 Re: 恭喜板斧上任
986 * upcspring Sep 6 Re: 恭喜板斧上任
987 * nemocell |
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m***n
发帖数: 266 | 50 ChemE的排名我较着其实挺tricky的.
要想排名高,招些做很fancy science的assistant prof
nano, bio, patterning/microfluidic, system biology,
fuel cell, biofuel, metabolic engineering等等,反正什么
热就招什么方向的的,funding自然多,也吸引本科生和研究生,
保证排名马上上去.可是这样做倒底有没有突出ChemE的特色和
长处,谁也不知道.
先说好啊,没有贬低上面那些方向的意思.关键是如果你是化工的人,
你就得拿出你看家的本领来从你不同于别人的角度来研究热点方向.
要不混然于其他众学科,就没法justify你这个单独学科存在的意义.
体外话,我一直以为
虽说90年代后 EE, CSE, MechE是工学院三大王牌军
但是 ChemE也不至于太差..
整了半天60-80年代在工学院绝对拉风的ChemE
现在成了拉大家后腿的差生了,faint.
就说新成员BioE吧,多少历史都来自ChemE啊.
ChemE老人给新人做嫁衣了... |
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