n******g
发帖数: 662 | 1 Did someone have experience on the biotinylation of monoclonal antibody?
I used Pierce kits, but it seems monoclonal antibody loses its activity. |
m*******h
发帖数: 896 | 2 completely loose activity?
Over biotinlyted?
【在 n******g 的大作中提到】
: Did someone have experience on the biotinylation of monoclonal antibody?
: I used Pierce kits, but it seems monoclonal antibody loses its activity.
|
n******g
发帖数: 662 | 3 It was debugged. Mab is over-biotinylated. |
w******n
发帖数: 13202 | 4 每个antibody对修饰程度的忍受度都不一样，所以对修饰的antibody要做一序列的修饰
筛选。具体而言，对于一定浓度的antibody，用1倍，2倍，。。。20倍biotin试剂来修
饰，然后用western blot从中找出最多biotin的活性antibody。
所以你的antibody失活，不是Pierce的错。 |
