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发帖数: 4 | 1 An alternative novel tool for DNA editing without target sequence limitation
Shu Xu†, Shasha Cao†, Bingjie Zou†, Yunyun Yue†,
Chun Gu, Xin Chen, Pei Wang, Xiaohua Dong, Zheng Xiang, Kai Li, Minsheng
ZhuEmail author, Qingshun ZhaoEmail author and Guohua Zhou
†Contributed equally
Abstract
Engineered endonucleases are a powerful tool for editing DNA. However,
sequence preferences may limit their application. We engineer a structure-
guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which
recognizes the 3′ flap structure, and the cleavage domain of Fok I (Fn1),
which cleaves DNA strands. The SGN recognizes the target DNA on the basis of
the 3′ flap structure formed between the target and the guide DNA (gDNA)
and cut the target through its Fn1 dimerization. Our results show that the
SGN, guided by a pair of gDNAs, cleaves transgenic reporter gene and
endogenous genes in zebrafish embryonic genome.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-1038-5 | c********6
发帖数: 693 | 2 但愿不是第二个韩春雨。看了这篇文章，感觉他所讲的优点很勉强，不是太有兴趣 |
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