m******5
发帖数: 1383 | 1 疑惑了，我本来觉得探针杂交后后面的immune detecting步骤就和ｗｅｓｔｅｒｎ应该
差不多
但发现ｃｏｍｍｅｒｃｉａｌ ｂｕｆｆｅｒ里都有马来酸 |
m******5
发帖数: 1383 | |
h******y
发帖数: 351 | 3 I asked the same question before on Histonet and other forum but never got a
satisfactory answer. :-(
I was doing in situ hybridization using TBS or PBS and came across another
protocol using Maleic acid. Then I went back and checked the data sheet
coming with the anti-DIG-AP antibody (Roche), and found that they suggested
using Maleic acid based buffer for "membrane application" and Tris-based
buffer for "other application".
Maleic acid has less buffering capacity than Tris in the pH range of 7-8,
but maleic acid buffer can be treated with DEPC-H2O, while Tris buffer can't.
There is a saying, "if it ain't broke, don't fix it".
【在 m******5 的大作中提到】
: 好奇地继续问……
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m******5
发帖数: 1383 | 4 噢，谢谢回复，顺便问一下，你用过Roche给的NBT/NICD底物么？平时用的时候它在膜
上显出要用的条带一般需要多久？ 以前没用过 |
h******y
发帖数: 351 | 5 我没有用过。Sorry。
【在 m******5 的大作中提到】
: 噢，谢谢回复，顺便问一下，你用过Roche给的NBT/NICD底物么？平时用的时候它在膜
: 上显出要用的条带一般需要多久？ 以前没用过
|
m******5
发帖数: 1383 | 6 ^_^ 你们一般用什么底物做southern 显AP conjugated antibody?
【在 h******y 的大作中提到】
: 我没有用过。Sorry。
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