m*****6
发帖数: 19 | 1 看到最近Stephen Quake的review：
Single-cell genome sequencing: current state of the science
其中提到：
。。。 two quite similar hybrid methods have been
developed. Both of these methods use a limited isothermal amplification
followed by PCR amplification of
the amplicons generated during the isothermal step
(FIG. 2c). As the name implies, ‘displacement DOP-PCR’
(also known as PicoPLEX) uses degenerate primers in
the first step to add a common sequence, followed by
priming of the common sequence for subsequent PCR
amplification. Most recently, multiple annealing and
looping-based amplification cycles (MALBAC) uses a
similar protocol, with the exception of using random
primers, as well as new common sequences and temperature cycling that are
claimed to promote looping
of the isothermal amplicons to inhibit further amplification before the PCR
step, which may result in more
uniform amplification.
不太明白尽管MALBAC有优势，但两种原理方法很类似的技术，PicoPLEX2002年就出来了
，为啥后面还能有MALBAC？ |
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