e****p
发帖数: 354 | 1 Pull down & Mass spec - a lot of proteins 60-80
Ask for help, - how to find the protein complex that my gene interacts with? |
A*****e
发帖数: 29 | 2 You need a database to exclude non-specific binding proteins. What epitope
did you tag on your protein? According to my experiences, more than 95%
proteins are not your interest. Your may try CRAPome online program to give
you a clue what are real hits. |
e****p
发帖数: 354 | 3 I used biotin-labeled - RNA pull down
thanks!
give
【在 A*****e 的大作中提到】
: You need a database to exclude non-specific binding proteins. What epitope
: did you tag on your protein? According to my experiences, more than 95%
: proteins are not your interest. Your may try CRAPome online program to give
: you a clue what are real hits.
|
C*********h
发帖数: 83 | 4 SILAC
with?
【在 e****p 的大作中提到】
: Pull down & Mass spec - a lot of proteins 60-80
: Ask for help, - how to find the protein complex that my gene interacts with?
|
