h********8
发帖数: 49 | 1 十几年前香港中文大的Dennis Lo的一篇孕妇外周血的文章，在测序的成本降到可接受
的水平后，成就了国内飞速发展的无创产前检测产业。
现在期待很久的癌症基因检测的文章来了，有可能改变国内下一代基因检测市场，想创
业的，你准备好了么？
Noninvasive detection of cancer-associated genome-wide hypomethylation and
copy number aberrations by plasma DNA bisulfite sequencing
PNAS 2013 ; published ahead of print November 4, 2013, doi:10.1073/pnas.
1313995110
Significance
Genome-wide hypomethylation is frequently observed in cancers. In this study
, we showed that genome-wide hypomethylation analysis in plasma using
shotgun massively parallel bisulfite sequencing is a powerful general
approach for the detection of multiple types of cancers. This approach is
particularly attractive because high sensitivity and specificity can be
achieved using low sequence depth, which is practical diagnostically. This
approach can also be used for monitoring patients following treatment. The
same sequencing data can be further used for detecting cancer-associated
copy number aberrations at no additional costs. One could thus combine
plasma hypomethylation and copy number analyses in a synergistic manner for
further enhancing detection sensitivity or specificity. | W***o
发帖数: 6519 | 2 wow, opportunity
study
【在 h********8 的大作中提到】
: 十几年前香港中文大的Dennis Lo的一篇孕妇外周血的文章，在测序的成本降到可接受
: 的水平后，成就了国内飞速发展的无创产前检测产业。
: 现在期待很久的癌症基因检测的文章来了，有可能改变国内下一代基因检测市场，想创
: 业的，你准备好了么？
: Noninvasive detection of cancer-associated genome-wide hypomethylation and
: copy number aberrations by plasma DNA bisulfite sequencing
: PNAS 2013 ; published ahead of print November 4, 2013, doi:10.1073/pnas.
: 1313995110
: Significance
: Genome-wide hypomethylation is frequently observed in cancers. In this study
| S*********s
发帖数: 304 | 3 plasma cancer gene detection一直就有,尤其是EGFR mutation.
这个系列有很多文章。只是sensitivity和false negative的问题。
如JCO上的这篇。是北大肿瘤医院王洁做的。
EGFR mutations detected in plasma are associated with patient outcomes in
erlotinib plus docetaxel-treated non-small cell lung cancer.
http://www.ncbi.nlm.nih.gov/pubmed/19414683
可以解决一些病人不做穿刺（实在是很痛苦）。
study
【在 h********8 的大作中提到】
: 十几年前香港中文大的Dennis Lo的一篇孕妇外周血的文章，在测序的成本降到可接受
: 的水平后，成就了国内飞速发展的无创产前检测产业。
: 现在期待很久的癌症基因检测的文章来了，有可能改变国内下一代基因检测市场，想创
: 业的，你准备好了么？
: Noninvasive detection of cancer-associated genome-wide hypomethylation and
: copy number aberrations by plasma DNA bisulfite sequencing
: PNAS 2013 ; published ahead of print November 4, 2013, doi:10.1073/pnas.
: 1313995110
: Significance
: Genome-wide hypomethylation is frequently observed in cancers. In this study
|
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