C***2
发帖数: 62 | 1 http://www.nature.com/ncb/journal/vaop/ncurrent/full/ncb2765.ht
Sequential introduction of reprogramming factors reveals a time-sensitive
requirement for individual factors and a sequential EMT–MET mechanism for
optimal reprogramming
Abstract:
Present practices for reprogramming somatic cells to induced pluripotent
stem cells involve simultaneous introduction of reprogramming factors. Here
we report that a sequential introduction protocol (Oct4–Klf4 first, then c-
Myc and finally Sox2) outperforms the simultaneous one. Surprisingly, the
sequential protocol activates an early epithelial-to-mesenchymal transition
(EMT) as indicated by the upregulation of Slug and N-cadherin followed by a
delayed mesenchymal-to-epithelial transition (MET). An early EMT induced by
1.5-day TGF-β treatment enhances reprogramming with the simultaneous
protocol, whereas 12-day treatment blocks reprogramming. Consistent results
were obtained when the TGF-β antagonist Repsox was applied in the
sequential protocol. These results reveal a time-sensitive role of
individual factors for optimal reprogramming and a sequential EMT–MET
mechanism at the start of reprogramming. Our studies provide a rationale for
further optimizing reprogramming, and introduce the concept of a sequential
EMT–MET mechanism for cell fate decision that should be investigated
further in other systems, both in vitro and in vivo. | t*d
发帖数: 1290 | 2 发的杂志差了点。发在 NB 就牛了，呵呵。
Here
c-
transition
【在 C***2 的大作中提到】
: http://www.nature.com/ncb/journal/vaop/ncurrent/full/ncb2765.ht
: Sequential introduction of reprogramming factors reveals a time-sensitive
: requirement for individual factors and a sequential EMT–MET mechanism for
: optimal reprogramming
: Abstract:
: Present practices for reprogramming somatic cells to induced pluripotent
: stem cells involve simultaneous introduction of reprogramming factors. Here
: we report that a sequential introduction protocol (Oct4–Klf4 first, then c-
: Myc and finally Sox2) outperforms the simultaneous one. Surprisingly, the
: sequential protocol activates an early epithelial-to-mesenchymal transition
|
|
