if to alternative splcing 3'-end multi ployA sites plus that alternative
ORF length are so longer than superscript reverse transcriptase can cover
likes 20kb then only
one choice is use that oligo dt and Multiscibe reverse transcriptase
then PCR different fragment by diffeeernt forward and reverse primer pairs
set then sub-cloning then sequencing then assembly different fragment to
one super long ORF.
【在 l**********1 的大作中提到】 : if to alternative splcing 3'-end multi ployA sites plus that alternative : ORF length are so longer than superscript reverse transcriptase can cover : likes 20kb then only : one choice is use that oligo dt and Multiscibe reverse transcriptase : then PCR different fragment by diffeeernt forward and reverse primer pairs : set then sub-cloning then sequencing then assembly different fragment to : one super long ORF. : : Multiscribe
