l**********1
发帖数: 5204 | 1 if to alternative splcing 3'-end multi ployA sites plus that alternative
ORF length are so longer than superscript reverse transcriptase can cover
likes 20kb then only
one choice is use that oligo dt and Multiscibe reverse transcriptase
then PCR different fragment by diffeeernt forward and reverse primer pairs
set then sub-cloning then sequencing then assembly different fragment to
one super long ORF.
Multiscribe |
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K*C
发帖数: 2825 | 2 本来是用来配Random primer的，dT好像应当用superscript，不知道dT和Multiscribe
能不能一起用 |
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w*****0
发帖数: 3349 | 3 is this english?
only
super |
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