o**a
发帖数: 86 | 1 Hi all,
Anyone knows of any way to separate the sense and antisense strands of
a 20~30 duplex? Base-paring can be broken by urea, and how to go after that?
Is HPLC possible? | s******9
发帖数: 283 | 2 some ways to do that. you need to tell your purpose. you can put a biotin
tag on one strand and use streptavidin beads to capture it . | b******n
发帖数: 4225 | 3 如果知道序列，找引物公司合成，肯定是单链，然后做你想做的
如果序列未知而且是混合物，末端连上tag，搞next generation sequencing搞清楚序
列之后再考虑是否合成
that?
【在 o**a 的大作中提到】
: Hi all,
: Anyone knows of any way to separate the sense and antisense strands of
: a 20~30 duplex? Base-paring can be broken by urea, and how to go after that?
: Is HPLC possible?
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