s*********y
发帖数: 579 | 1 We usually cut a piece of tissue and put in RNAlater. This way we can
isolate RNA later.
This time I got some tissue from other lab. They just keep tissue in -80
degree.Has anybody extract RNA from them? How is the quality? |
C*********u
发帖数: 811 | 2 It depends on which tissue...
For liver, brain, muscle, white fat and lung, I got very good quality of RNA
from them. But for pancreas, no...
【在 s*********y 的大作中提到】
: We usually cut a piece of tissue and put in RNAlater. This way we can
: isolate RNA later.
: This time I got some tissue from other lab. They just keep tissue in -80
: degree.Has anybody extract RNA from them? How is the quality?
|
s*********y
发帖数: 579 | 3 They are lung tissues. But they are not in Trizol or RNAlater.I think they
just freeze the tissue. So I don't want to try.
Pacrease is hard to get good quality RNA, even fresh tissue. |
w***a
发帖数: 4361 | 4 For lung, no problem to flash freeze the sample and do Trizol extraction
later.
【在 s*********y 的大作中提到】
: They are lung tissues. But they are not in Trizol or RNAlater.I think they
: just freeze the tissue. So I don't want to try.
: Pacrease is hard to get good quality RNA, even fresh tissue.
|
s***o
发帖数: 11 | 5 同意。液氮下研磨后加Tizol提取没问题。
我们存了几千份肿瘤标本，都是离体后马上丢到液氮里，然后转到-80冰箱长期保存。
如果离体后速度降温，中间没有反复升降温度，十年以上都没有问题。
当然有些组织Rnase特别丰富的需要小心些，比如胰腺。
【在 w***a 的大作中提到】
: For lung, no problem to flash freeze the sample and do Trizol extraction
: later.
|
s*********y
发帖数: 579 | 6 I know it's OK to do quick-freeze in liquid nitrogene and leave in -80.
But they just cut the tissue and leave in -80.I guess it's not good. |
