d****i
发帖数: 2346 | | a***e
发帖数: 1010 | | d****i
发帖数: 2346 | 3 我是想把目的蛋白和核定位信号序列融合构成plasmid，希望转染后表达的蛋白都进到
细胞核内。这种融合的时候在NLS和目的基因之间要加其他序列吗？不会就这么硬生生
的连在一起吧？谢谢! | b******y
发帖数: 627 | 4 Check Yuh Min Chook's studies. There are many options. Of course, the one
suggested by ahche works. As far as linkers are concerned, a GGS linker in
between should do it. If your protein is intrinsically disordered at the
location of linkage, direct fusion should also do it. What matters is the
availability of the NLS to importin(s). |
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