m******5
发帖数: 1383 | 1 大家一般都筛多久呢？
筛出来有多少是干净的homozygote?
我用6mg G418 筛了25天就6，7个克隆…… |
i*********0
发帖数: 915 | 2 6mg, this is really high?
which cell type are you using? |
m******5
发帖数: 1383 | 3 mES cell
by the way, you still didn't answer the last question regarding whether or
not your mES cell could go into germ line………… I am really curious about
it.
【在 i*********0 的大作中提到】
: 6mg, this is really high?
: which cell type are you using?
|
i*********0
发帖数: 915 | 4 we did not try to get a germline transmission. |
y******8
发帖数: 1764 | 5 You may get undesired integration. |
g*********5
发帖数: 2533 | 6 usually how long could you maintaining the mES cells?
about
【在 m******5 的大作中提到】
: mES cell
: by the way, you still didn't answer the last question regarding whether or
: not your mES cell could go into germ line………… I am really curious about
: it.
|
m******5
发帖数: 1383 | 7 could you make your question more specific? I don't get it……
【在 g*********5 的大作中提到】
: usually how long could you maintaining the mES cells?
:
: about
|
