p**5 发帖数: 2544 | 1 can anyone recommend a good one and where to order? Thanks.
want to dialysis a peptide (to remove DTT)
gel filtration may not be sufficient in removing DTT |
a****k 发帖数: 1130 | 2 try this, we ordered from Fisher
http://www.spectrumlabs.com/dialysis/RCtubing.html
【在 p**5 的大作中提到】 : can anyone recommend a good one and where to order? Thanks. : want to dialysis a peptide (to remove DTT) : gel filtration may not be sufficient in removing DTT
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a****k 发帖数: 1130 | 3 btw, you also need to order Spectra/Por Closures
http://www.spectrumlabs.com/dialysis/RegClose.html
【在 p**5 的大作中提到】 : can anyone recommend a good one and where to order? Thanks. : want to dialysis a peptide (to remove DTT) : gel filtration may not be sufficient in removing DTT
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b******y 发帖数: 627 | 4 I guess peptide column http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165421 should be able to separate most peptide from DTT or BME unless your peptide is only a few mer. |
p**5 发帖数: 2544 | 5 thanks. I will order this one.
I also see this online, but not sure about it. http://shop.midsci.com/productdetail/M50/IB48302
【在 a****k 的大作中提到】 : try this, we ordered from Fisher : http://www.spectrumlabs.com/dialysis/RCtubing.html
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p**5 发帖数: 2544 | 6 Thanks, good to know.
My peptide is 26 AA long, but I still worry DTT may not be completely
removed.
【在 b******y 的大作中提到】 : I guess peptide column http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165421 should be able to separate most peptide from DTT or BME unless your peptide is only a few mer.
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b******y 发帖数: 627 | 7 it should be ok. Or use bme instead, which is half of dtt. |
a****k 发帖数: 1130 | 8 sorry,刚刚看成10K了,1k的话看看这些吧
http://eu.spectrumlabs.com/dialysis/RCtreat.html?Pn=132103;
我们一直用的是这家的
【在 p**5 的大作中提到】 : Thanks, good to know. : My peptide is 26 AA long, but I still worry DTT may not be completely : removed.
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c******6 发帖数: 928 | 9 there are two types of tubes, one is organic solvent-resistant, the other
one is not and can only be used for dialysis in aqueous environment. |
p**5 发帖数: 2544 | 10 没关系,在那里我看到1K的了,就是这个132103合适,16 foot length合适
【在 a****k 的大作中提到】 : sorry,刚刚看成10K了,1k的话看看这些吧 : http://eu.spectrumlabs.com/dialysis/RCtreat.html?Pn=132103; : 我们一直用的是这家的
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p**5 发帖数: 2544 | 11 I want to use tcep, which is stabler and less buffer issues.
【在 b******y 的大作中提到】 : it should be ok. Or use bme instead, which is half of dtt.
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