m*******n
发帖数: 387 | 1 推测某个非histone的蛋白可能被甲基化
想用pan-methylation-K的抗体试试，当然是IP之后
求推荐一个比较好用的抗体
或者还有其他比较好的方法，证明某个蛋白可以被甲基化吗？ | g****e
发帖数: 137 | 2 the most classic way to do this is to use radioactive SAM or stable isotope
labeled methyl group donor. There are many pan-methylation antibody
available, but don't expect too much for them. Unlike acetylation and many
other PTM, methylation has mono, di and tri-methylation, that's why is
difficult to develop an antibody recognize all three types of methylation. | m*******n
发帖数: 387 | 3 谢谢回复
那要是把这个蛋白纯化出来，做质谱如何？
isotope
【在 g****e 的大作中提到】
: the most classic way to do this is to use radioactive SAM or stable isotope
: labeled methyl group donor. There are many pan-methylation antibody
: available, but don't expect too much for them. Unlike acetylation and many
: other PTM, methylation has mono, di and tri-methylation, that's why is
: difficult to develop an antibody recognize all three types of methylation.
| g****e
发帖数: 137 | 4
Yes, it's doable but it's not as sensitive as isotope labeled protein.
【在 m*******n 的大作中提到】
: 谢谢回复
: 那要是把这个蛋白纯化出来，做质谱如何？
:
: isotope
|
|
