c**i
发帖数: 6973 | 1 Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
cheaper way to sequence genome. New York Times, July 21, 2011.
http://www.nytimes.com/2011/07/21/science/21genome.html
Excerpt in the window of print: It will take years to make sense of much of
teh data a novel technology reveals.
My comment:
(a) Techmnology. Ion Torrent, undated.
http://www.iontorrent.com/technology-scalability-simplicity-spe
Go to "How does it work?" in the left column. Clcik "Read more" and you
reach the text. By comparing the video clip, it is clear that the sequence
of the DNA for the last several nucleosides are 5'-T-G-A-C-T-T-3'.
(b) To be frank, I was not taught that a hydrogen ion would be released. The
following is what we are taught in biochemistry.
See the figure at page 866, in
Reginald Garrett and Charles M. Grisham. Biochemistry, fourth edition.
publisher: Mary Finch, 2010.
http://books.google.com/books?id=iGPsen3fSOIC&pg=
PA866&lpg=PA866&dq=%22dna+polymerase%22+elongation
+5'+biochemistry+%223'+oh%22&source=bl&ots=P9_lkwuZ03&sig
=Jl74E6oEjomoKueuR6F7XWyAO-Q&hl=en&ei=UHkoTuHyH8idgQeGlLRc&sa
=X&oi=book_result&ct=result&resnum=2&ved=0CBwQ6AEwAQ#v=onepage&q&f=false
("FIGURE 28.4 The chain elongation reaction catalyzed by DNA polymerase.The
3-OH carries out a nucleophilic attack on the -phosphoryl group of the
incoming dNTP to form a phosphoester bond, and PP is released. The
subsequent hydrolysis of PP, by inorganic pyrophosphatase renders teh
reaction effectively irreversible")
* dNTP is a short hand for deoxyNucleoside TriPhosphate. See the left panel
of the first, color graphic in
http://en.wikipedia.org/wiki/Nucleotide
* For laypersons, DNA elongation proceeds from 5' to 3'. The the phosphate of
the incoming dNTP would combine with the hydroxyl (OH) of 3' end. | w******e
发帖数: 1187 | 2 ion torrent 不新了吧？throughput还是低了点。
,
of
【在 c**i 的大作中提到】
: Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
: cheaper way to sequence genome. New York Times, July 21, 2011.
: http://www.nytimes.com/2011/07/21/science/21genome.html
: Excerpt in the window of print: It will take years to make sense of much of
: teh data a novel technology reveals.
: My comment:
: (a) Techmnology. Ion Torrent, undated.
: http://www.iontorrent.com/technology-scalability-simplicity-spe
: Go to "How does it work?" in the left column. Clcik "Read more" and you
: reach the text. By comparing the video clip, it is clear that the sequence
| s******s
发帖数: 13035 | 3 这个版上都讨论了一年多了
【在 w******e 的大作中提到】
: ion torrent 不新了吧？throughput还是低了点。
:
: ,
: of
| m***T
发帖数: 11058 | 4 主要是第一篇介绍ION技术的paper刚在nature上发表了。公司的marketing自然不会放
过这么好的机会 | f**********e
发帖数: 1994 | 5 这方法通量不行，library prep 和 solid 一样 ”好“。 | e*******e
发帖数: 1837 | 6 This is a more insightful post if you care about the study:
How accurate is the new Ion Torrent genome, really?
http://m.wired.com/wiredscience/2011/07/how-accurate-is-the-new
,
of
【在 c**i 的大作中提到】
: Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
: cheaper way to sequence genome. New York Times, July 21, 2011.
: http://www.nytimes.com/2011/07/21/science/21genome.html
: Excerpt in the window of print: It will take years to make sense of much of
: teh data a novel technology reveals.
: My comment:
: (a) Techmnology. Ion Torrent, undated.
: http://www.iontorrent.com/technology-scalability-simplicity-spe
: Go to "How does it work?" in the left column. Clcik "Read more" and you
: reach the text. By comparing the video clip, it is clear that the sequence
| p****r
发帖数: 148 | 7 大家对Pacific bio和Complete Genome的技术怎么看？ | f**********e
发帖数: 1994 | |
|
