J********n
发帖数: 534 | 1 版上有用这个cytokine的人么？
最近需要>1 mg的蛋白，做一些binding study。查了查，1个mg都要5000+。
有人能推荐推荐质量过关，价格又比较便宜的vendor么？
E.coli refolded or insect cell expressed的都可以。 |
s****9
发帖数: 932 | 2 Peprotech, cheapest. Works fine for M-CSF induced BM macrophage in vitro.
For 1mg cytokine, best to purify by yourself unless your lab is super rich.
【在 J********n 的大作中提到】
: 版上有用这个cytokine的人么？
: 最近需要>1 mg的蛋白，做一些binding study。查了查，1个mg都要5000+。
: 有人能推荐推荐质量过关，价格又比较便宜的vendor么？
: E.coli refolded or insect cell expressed的都可以。
|
J********n
发帖数: 534 | 3 $5000 for 1mg?
Too expensive.
Do you have the E.coli expression plasmid?
May I get some from you? I have the refolding protocol.
vitro.
rich.
【在 s****9 的大作中提到】
: Peprotech, cheapest. Works fine for M-CSF induced BM macrophage in vitro.
: For 1mg cytokine, best to purify by yourself unless your lab is super rich.
|
s****9
发帖数: 932 | 4 Have the cell line reduced with M-CSF. Just used the culture supernatant
for culturing macrophage purpose. It is not tagged and thus I am concerned
that it may not fit for your purpose.
【在 J********n 的大作中提到】
: $5000 for 1mg?
: Too expensive.
: Do you have the E.coli expression plasmid?
: May I get some from you? I have the refolding protocol.
:
: vitro.
: rich.
|
