s****z
发帖数: 50 | 1 准备用real-time PCR 检侧一个RNA在细胞核和细胞质中的分布。
请教板上的大虾，用什么做internal control 比较合适啊？
似乎18S, tubulin, etc, 都不好 | e*r
发帖数: 103 | | s****z
发帖数: 50 | 3 Is histone mRNA equally distributed in nuclei and cytoplasm? | c*******s
发帖数: 29 | 4 I think you should use the same proportion of the nuclear RNA and
cytoplasmic RNA for the RT-PCR. What's the purpose of your control? You can
do RT-PCR for an pre-mRNA (containing introns which should be exported) to
show your fractionation is clean.
【在 s****z 的大作中提到】
: 准备用real-time PCR 检侧一个RNA在细胞核和细胞质中的分布。
: 请教板上的大虾，用什么做internal control 比较合适啊？
: 似乎18S, tubulin, etc, 都不好
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