anybody used multisite gateway vectors to generate knockout cell lines? - Biology版

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Biology版 - anybody used multisite gateway vectors to generate knockout cell lines?

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● Cre line to generate global knockout	● induceable lentiviral vector
● 也问gateway vector: 有没有可以同时放shRNA和cDNA的	● 两组时间序列的比较
● 请问一个gateway clone问题	● [转载] Re: 有谁知道记忆是怎么回事吗? 拜托各位
● 有没有人用multisite gateway做克隆的？	● Re: A question about KO
● multisite gateway pro cloning kit from Invitrogen	● GENE KNOCKOUTS LISTED
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● 该如何分开与insert大小相近的vector呢？	● 工科的转作生物，有哪些合适的方向呢？

相关话题的讨论汇总
话题: multisite话题: anybody话题: vectors

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w******d
发帖数: 46

There are some publications about that. I am just wondering if anybody have
some personal experience with this system. Is it easy to work with?
Thanks!

(共1页)

进入Biology版参与讨论

相关主题
● 工科的转作生物，有哪些合适的方向呢？	● multisite gateway pro cloning kit from Invitrogen
● 做发育的给科普一下	● 大家给推荐个做multisites-directed mutagenesis 的kit吧
● 新手请教 'Genotype-phenotype correlation’	● stable co-transfection
● 关于RNAi Knockdown的efficiency	● 该如何分开与insert大小相近的vector呢？
● Cre line to generate global knockout	● induceable lentiviral vector
● 也问gateway vector: 有没有可以同时放shRNA和cDNA的	● 两组时间序列的比较
● 请问一个gateway clone问题	● [转载] Re: 有谁知道记忆是怎么回事吗? 拜托各位
● 有没有人用multisite gateway做克隆的？	● Re: A question about KO

相关话题的讨论汇总
话题: multisite话题: anybody话题: vectors

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