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发帖数: 134 | 1 Could anyone please help me with some conditional KO questions?
Our lab have flox-transcription factor X-flox mice. I am thinking of
crossing these mice with some kind of
cre mice for conditional knockout of X . My purpose is to study the down
stream target of X. Specifically, I plan
to culture the cells in vitro , induce deletion of gene A in vitro, and to
examine gene expression between WT
and X KO cells. My questions are :
1) I hear that the efficiency of inducing conditional KO in vitro is not
very high. Is that true? Do I have to use
Rosa-GFP or some other kind of reporter to differentiate GFP+ and GFP- cells
? My cells are hematopoietic cells
, so I will be able to isolate GFP+ cells from GFP- cells by flow cytometry.
2) Is there any good cre mouse database that I can check and see which cre
mice is available.
Xie Xie!!!!! |
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