s**u
发帖数: 9035 | 1 I am using DAKO monoclonal mouse anti-human ki-67 antibody(clone MIB-1) to
do IHC stainng in mouse tumor tissues.
Is concentration 1:100 good, I am using ABC kit, DAB 20 seconds, hematoxylin
6 minutes good?
Do you have suggestions? | v***a
发帖数: 1242 | 2 IHC这玩意就只能自己多试
我觉得最重要的是得有一个好antibody
一般我是多查paper看有没有前人用过的
不同的动物或组织染出来还不一样
DAB的时间长短也不一定
要根据显色反应的程度来进行判断
我是几秒到10几分钟都有过
hematoxylin
【在 s**u 的大作中提到】
: I am using DAKO monoclonal mouse anti-human ki-67 antibody(clone MIB-1) to
: do IHC stainng in mouse tumor tissues.
: Is concentration 1:100 good, I am using ABC kit, DAB 20 seconds, hematoxylin
: 6 minutes good?
: Do you have suggestions?
| M*****e
发帖数: 279 | 3 If you are interested in cell proliferation, phosphorylated Histone H3 S10
antibody is better.
Ki67 is not M-phase specific. Histone H3 pS10 is relatively M-phase specific.
You can use 1:50 to 1:100 for Histone H3 pS10 on Formalin fixed paraffin
embedded tissue section. You can recycle the antibody (add sodium azide) and
re-use it later. |
|
