s*****g
发帖数: 7857 | 1 用invetrogen的胶和膜.谢谢!
电压?
电流?
时间? |
I*****y
发帖数: 6402 | 2 1 minute per kD at the constant current of 300 mA. YOu better use PVDF
membrane, and keep the transfer buffer cold during the transfer.
【在 s*****g 的大作中提到】
: 用invetrogen的胶和膜.谢谢!
: 电压?
: 电流?
: 时间?
|
s*****g
发帖数: 7857 | |
g*****y
发帖数: 6325 | 4 我过去的实验室都是在冰上transfer overnight.
【在 I*****y 的大作中提到】
: 1 minute per kD at the constant current of 300 mA. YOu better use PVDF
: membrane, and keep the transfer buffer cold during the transfer.
|
I*****y
发帖数: 6402 | 5 we do it in our 4 degree cold room. Overnight transfer for western is not
common doing, most power supplies have a timer nowadays.
【在 g*****y 的大作中提到】
: 我过去的实验室都是在冰上transfer overnight.
|
a***a
发帖数: 40617 | 6 偷懒都用overnight啊
【在 I*****y 的大作中提到】
: we do it in our 4 degree cold room. Overnight transfer for western is not
: common doing, most power supplies have a timer nowadays.
|
s********x
发帖数: 472 | 7 most people do overnight transfer, and there are so many shakers and frigdes
need power all day.
【在 I*****y 的大作中提到】
: we do it in our 4 degree cold room. Overnight transfer for western is not
: common doing, most power supplies have a timer nowadays.
|
g*********5
发帖数: 2533 | 8 7min with iblot and use INVITROGEN 3-8% TRIS ACETATE GEL. NO PROBELM with
mTor, 280kDa. |
