T**********t 发帖数: 1604 | 1 好久没做miniprep了,实验室换了好几茬儿kit我都不知道。
这两天想做几个miniprep来着,可是发现一堆盒子里都没有qiaprep spin column,只
在冰箱里找到一些MinElute Spin Column,不知道可不可以换着用啊?
不行的话,估计就只能订新的kit了。 | T**********t 发帖数: 1604 | 2 看了一下Qiagen网上的faq,好像不行,虽然没提MinElute,可是估计道理一样的:
Are QIAprep and QIAquick Spin columns interchangeable?
No. While columns from the QIAprep Spin Miniprep Kit and the QIAquick PCR
Purification- and Gel Extraction Kits are based on silica-gel-membrane
technology, each is designed to work optimally within its own kit format. In
addition, the binding capacity and DNA recovery size cut-offs of the
QIAprep and QIAquick Spin columns are different. QIAprep Spin columns bind
up to 20 ug of plasmid DNA up to |
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