由买买提看人间百态

boards

本页内容为未名空间相应帖子的节选和存档,一周内的贴子最多显示50字,超过一周显示500字 访问原贴
Biology版 - Re: Help! Lentiviral expression system
相关主题
siRNA初级问题请教any trick to PCR tandem GFP?
请推荐一个lentiviral vector能表达两个gene+shRNAmammalian cells transfection 后 integration 的效率大概是多少?
被慢病毒折腾惨了,大家帮忙看看吧大家有什么好的lentival vector推荐一下么
lentiviral based Cell line 求助做实验室 碰到伤口了
花了一个月克隆LncRNA,发现一个大问题,我想跳楼。。。用lentiviral vector对操作者的危害有多大啊?
induceable lentiviral vectorHas anyone made pGIPZ lentiviral KD construction before?
5个包子求解释文章的一个实验结果-Flow cytometry analysisshRNA and Lentiviral Particle Gene Silencers
Direct reprogramming from ES or iPS cells to somatic cells by TF ectopic expression可行吗?请问如何筛选悬浮细胞的稳定株
相关话题的讨论汇总
话题: lentiviral话题: expression话题: system话题: why话题: gfp
进入Biology版参与讨论
1 (共1页)
w****t
发帖数: 1
1

If things happened like that, why not design a control such as GFP insterted
into your vector, so it is easy to check the infection efficiency. It is not
surprised some cells survived after drug selection, there is why you need to
confirm the ectopic expression of your targer gene in stable cell line by WB
or other ways.
1 (共1页)
进入Biology版参与讨论
相关主题
请问如何筛选悬浮细胞的稳定株花了一个月克隆LncRNA,发现一个大问题,我想跳楼。。。
请教lentiviral vector转染neuronal cultureinduceable lentiviral vector
RNAi knockdown5个包子求解释文章的一个实验结果-Flow cytometry analysis
求paperDirect reprogramming from ES or iPS cells to somatic cells by TF ectopic expression可行吗?
siRNA初级问题请教any trick to PCR tandem GFP?
请推荐一个lentiviral vector能表达两个gene+shRNAmammalian cells transfection 后 integration 的效率大概是多少?
被慢病毒折腾惨了,大家帮忙看看吧大家有什么好的lentival vector推荐一下么
lentiviral based Cell line 求助做实验室 碰到伤口了
相关话题的讨论汇总
话题: lentiviral话题: expression话题: system话题: why话题: gfp