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Biology版 - Re: Need help please on WB
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8楼更新提问,关于用苯清洗的替代问题色谱中的载荷量和上样量是一个意思吗? (转载)
Re: 变态老师要求用EB染胶请问有没有好的immunofluorescence on tissue cultures cells的protocol?
How to Destain In situ hybridization?Do you guys reuse the WB transfer buffer?
哪个公司的WB dry transfer system比较好用?有没有人做western用Biorad的半干转移,给点指示吧
Biofuels/energy research directions/postdoc positionwestern转膜见鬼了
含KCl的蛋白跑SDSPAGE胶遇到沉淀怎么办?问个ethanol-dry ice bath的问题
问一下methanol/acetic acid的fixation大家碰到过膜蛋白在胞质中染色的吗?
能不能用可逆的 fixation 做 RPPA?【求助】 有图:蛋白胶跑成这样,会是什么原因?
相关话题的讨论汇总
话题: need话题: wb话题: 1ab话题: 2ab话题: washed
进入Biology版参与讨论
1 (共1页)
m*t
发帖数: 1
1
Let me make a bold guess just for fun. :)))
Assume that the band you have seen after 2Ab is the correct band instead of a
crossreactivity artifact, I guess what was happening is that you 1Ab has a
very weak interaction against the fusion protein. The Ab can be easily washed
off in the subsequent washing steps, however, when you added destain solution,
the methanol fixes the 1Ab on the blot so that it woon't be totally washed.
Therefore the 2Ab can detect the 1Ab on the membrane, and that's also
1 (共1页)
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【求助】 有图:蛋白胶跑成这样,会是什么原因?Biofuels/energy research directions/postdoc position
EGFP免疫荧光固定?含KCl的蛋白跑SDSPAGE胶遇到沉淀怎么办?
为啥做个phalloidin染actin都做不出来[求助]问一下methanol/acetic acid的fixation
问一个 IF 的问题能不能用可逆的 fixation 做 RPPA?
8楼更新提问,关于用苯清洗的替代问题色谱中的载荷量和上样量是一个意思吗? (转载)
Re: 变态老师要求用EB染胶请问有没有好的immunofluorescence on tissue cultures cells的protocol?
How to Destain In situ hybridization?Do you guys reuse the WB transfer buffer?
哪个公司的WB dry transfer system比较好用?有没有人做western用Biorad的半干转移,给点指示吧
相关话题的讨论汇总
话题: need话题: wb话题: 1ab话题: 2ab话题: washed