How to Destain In situ hybridization? - Biology版 - 未名存档

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Biology版 - How to Destain In situ hybridization?

相关主题
● anybody has experience in zebrafish insitu hybridization?	● 请推荐做In situ hybridization的公司
● Northern Blot用Dig-labelled探针Li-cor检测，可行吗？	● 请问做section in situ hybridization 怎样能提高灵敏度？
● Re: Who has experience in doing In-situ Hybridization?	● 有啥公司能给做in situ hybridization 用的plasmid
● 问个技术问题。。frozen section 做in situ hybridization	● 发育生物学Phd找Industry job
● 有人做过quantatative immunofluorescence 么？	● 请教：有没有公司可以做in situ hybridization服务的
● Roche的DIG in situ system 怎么样？	● 检测某个基因的产物(RNA/protein)的位置有什么好办法?
● 请问有人找过公司做in situ hybridization的么？	● 如何定位细胞释放因子？
● 请教做long bone section 的高手	● Bay Area Biotech Scientist Position

相关话题的讨论汇总
话题: situ话题: destain话题: destaining话题: nbt

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M*****n 发帖数: 16729	1 Hi, Anybody has experience in destaining BCIP/NBT coloration product? my in situ gave a pretty high background, hope it may be rescued by destaining. Thanks very much.
c*********r 发帖数: 1312	2 【在 M*****n 的大作中提到】 : Hi, Anybody has experience in destaining BCIP/NBT coloration product? : my in situ gave a pretty high background, hope it may be rescued by : destaining. : Thanks very much.
s****9 发帖数: 932	3 95% Etoh for 30-60 mins. 【在 M*****n 的大作中提到】 : Hi, Anybody has experience in destaining BCIP/NBT coloration product? : my in situ gave a pretty high background, hope it may be rescued by : destaining. : Thanks very much.
M*****n 发帖数: 16729	4 thanks guys, will try tomorrow.
F*****e 发帖数: 182	5 泡在PBST（0.1% Tween-20)里，4度放几天，可以降低一些background。
j*****d 发帖数: 787	6 too long! try 3 minutes, 6 minutes, 10 minutes..... 【在 s****9 的大作中提到】 : 95% Etoh for 30-60 mins.

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相关主题
● Bay Area Biotech Scientist Position	● 有人做过quantatative immunofluorescence 么？
● RNA in situ hybridization请教	● Roche的DIG in situ system 怎么样？
● in situ hybridization做不好，有没有好的protocol？	● 请问有人找过公司做in situ hybridization的么？
● Re: 变态老师要求用EB染胶	● 请教做long bone section 的高手
● anybody has experience in zebrafish insitu hybridization?	● 请推荐做In situ hybridization的公司
● Northern Blot用Dig-labelled探针Li-cor检测，可行吗？	● 请问做section in situ hybridization 怎样能提高灵敏度？
● Re: Who has experience in doing In-situ Hybridization?	● 有啥公司能给做in situ hybridization 用的plasmid
● 问个技术问题。。frozen section 做in situ hybridization	● 发育生物学Phd找Industry job

相关话题的讨论汇总
话题: situ话题: destain话题: destaining话题: nbt

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