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Biology版 - [合集] 请问saponin透化细胞做免疫染色有什么好处?
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Re: 求助!GST fusion protein cleavage-thrombinimmunoprecipitation 请教.
请教做long bone section 的高手请教Ab Ag binding的问题
请大家看看这样做Co-IP是否靠谱VWR 的CO2 incubator好用吗?
Flow cytometry: permeability agent for DNA probesDNA damage FPG assay请教
有人用sulfo-NHS-LC-biotin吗?Re: GST-Fusion 蛋白提纯求助!!!
Interesting report in NatureRe: western blotting杂带太多怎么办?
请推荐一个用来incubate enzyme 和底物的incubator大家再来个PVDF western大讨论好吗?
【发包子】请ELISA高手进3 ways Re: 请问怎样浓缩浓度过
相关话题的讨论汇总
话题: saponin话题: 透化话题: 免疫染色话题: ab话题: trition
进入Biology版参与讨论
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bewithu (x-fire in the sea) 于 (Sun Dec 31 10:38:41 2006) 提到:
For regular immunofluorescence, Trition may be good enough (such as 0.1% in
PBS, 10-15 min, RT). Saponin, which is generally purified from plants, is a
much weaker detergent. It is believed that the permeabilzation effect given
by saponin is reversible, thus you need to use saponin-containing solutions
for all steps during IF (Ab incubation and washing).
The advantage is that saponin will gi
1 (共1页)
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3 ways Re: 请问怎样浓缩浓度过有人用sulfo-NHS-LC-biotin吗?
Re: How long does it take for expressionInteresting report in Nature
Re: 请教免疫荧光请推荐一个用来incubate enzyme 和底物的incubator
Re: 免疫荧光对照有染色怎搞?【发包子】请ELISA高手进
Re: 求助!GST fusion protein cleavage-thrombinimmunoprecipitation 请教.
请教做long bone section 的高手请教Ab Ag binding的问题
请大家看看这样做Co-IP是否靠谱VWR 的CO2 incubator好用吗?
Flow cytometry: permeability agent for DNA probesDNA damage FPG assay请教
相关话题的讨论汇总
话题: saponin话题: 透化话题: 免疫染色话题: ab话题: trition