s********8
发帖数: 619 | 1 那倒是,但是好像2A还是比IRES要靠谱些,至少翻译的时候没差太远. |
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c********n
发帖数: 67 | 2 In vivo real-time visualization of leukocytes and intracellular hydrogen
peroxide levels during a zebrafish acute inflammation assay.
PMID:22341223
h***[email protected]
Thanks very much :) |
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c********n
发帖数: 67 | 3 Research progress of zebrafish as a model system for hematological neoplasms
Thanks very much
h***[email protected] |
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G*****m
发帖数: 198 | 4 zebrafish 比较省钱，你们这堆已经/即将开始自己lab的人里面，有做斑马鱼神经系统
的么？ |
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r*****g
发帖数: 478 | 5 差点被你的翻译吓到了。原来是说zebrafish |
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f*********a
发帖数: 46 | 6 Full-time Associate Professor or Full Professor
The Model Animal Research Center (MARC) of Nanjing University invites
applications for* full-time faculty positions, at academic ranks of
Associate or Full Professor.* Individuals with demonstrated accomplishments
in, but not limited to, the following areas are encouraged to apply –
neurobiology, immunology, development biology, metabolic disease and cancer
biology. Highly competitive research support will be provided in an
interactive and nurturin... 阅读全帖 |
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c********n
发帖数: 67 | 7 New insights into signaling during myelination in zebrafish
Thanks for your help
h***[email protected] |
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w***y
发帖数: 493 | 8 我现在正在尝试从zebrafish embryo的total RNA里面提取mRNA， 用的是invitrogen的
Dynabeads和他们的mRNA purification kit。 按照他们的protocol做完后跑胶发现里
面还是有挺多rRNA。上网搜了一下发现有的人建议连续做2次protocol里说的
purification。 请问大家有什么建议么？
我试了一下发现第1次纯化后获得了大概3.8%的total RNA。第二次以后有1.5%的total
RNA。我不太确定这样连续2次purification会不会损失太多mRNA。 |
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w***y
发帖数: 493 | 9 谢谢， 我只想要mRNA，因为我要去做mRNA-seq, 所以要去掉rRNA。 我看了rRNA-zero
kit，发现这是用在Human/Mouse/Rat上的， 似乎不能确定是否能用在zebrafish上，在
网上搜到有人说在鱼里效果不怎么样。 请问你是用在鱼里么？另外这个kit会不会导致mRNA的丢失呢？
about 5ug and ends up with 150- |
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l**********1
发帖数: 5204 | 10 alternatively you can try Oligotex-dt30 mRNA purification kit for total RNA
sample:
Manual book:
//catalog.takara-bio.co.jp/PDFFiles/NTrap%20mRNA_j.pdf
Vender link:
//www.mn-net.com/Products/DNAandRNApurification/RNA/NucleoTrapmRNA/tabid/1328/language/en-
US/Default.aspx
Reference:
please go to
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/18678863
T Yabu - 2008 JBC
31 Oct 2008 – In zebrafish embryonic cells, the endogenous SMase enzyme was
.... using the Oligotex-dt30 mRNA purification kit (Tak... 阅读全帖 |
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n***w
发帖数: 2405 | 12 sent
next time put the article title in the main text. :-) |
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m*********r
发帖数: 136 | 13 Embedding, serial sectioning and staining of zebrafish embryos using JB-4
resin
Thanks
y******[email protected] |
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M*****n
发帖数: 16729 | 14 neverthink 老大，同济现在给什么价码？
刚刚发现一个研究zebrafish的朋友也在同济拿到professor了. |
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S*********e
发帖数: 127 | 15 check Mary Goll's recent work on Gal4-UAS system in zebrafish. |
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l******e
发帖数: 427 | 17 Faculty Positions in Chronobiology
Multiple faculty positions in the broad areas of chronobiology at Assistant
/Associate /Full Professor level are available in Soochow University Center
for Circadian Clocks (SUCCC) in Suzhou, China.
We are seeking outstanding scholars who use fruit fly, zebrafish, mouse or
human to investigate molecular genetic mechanisms underlying circadian
regulation, or study regulatory roles of circadian clocks in development,
reproduction, metabolism, sleep, behaviors, im... 阅读全帖 |
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h**********r
发帖数: 671 | 18 td-Tomato和mcherry都可以，用tac或者trc promoter都可以，不想用IPTG诱导的话，
去掉或者破坏lacI
可以看看这个AEM
Broad-Host-Range Plasmids for Red Fluorescent Protein Labeling of
Gram-Negative Bacteria for Use in the Zebrafish Model System
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2010, p. 3467–3474 Vol. 76, No
. 11
doi:10.1128/AEM.01679-09 |
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c*********r
发帖数: 1312 | 19 谢谢shyoung姐姐支持！
就再多啰嗦几句吧。当时写这句话也是想着照顾对方感情，想把事情做圆满些。文化差
异吧。shyoung的回复又让我学会了以后类似情况该怎么处理！
顺带回复一下wonderlich，不知他还是否会看到。关于拉选票，确实也是和对手学的。
不过至少我还会在自己帖子里夸一下每个参选作品的优点。对手在facebook里则简单明
了的只说Vote for xxx's turtle!!!。而且互相吹捧"My, what a handsome turtle!!"
呵呵，要说sick，他们也不会更好吧。
转发一下当时课程director对本轮投票的email：
“Hi All,
Hope you all are doing well. The fourth (and final) round of four pictures
up for a Development cover have now been posted at the Node.
http://thenode.biologists.com/vote-for-a-development-cover-wood
Pl... 阅读全帖 |
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a***r
发帖数: 58 | 20 感觉是用zebrafish转基因来构建水质检测或者评估的模型 |
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b****n
发帖数: 110 | 21 Faculty Positions in Chronobiology
Multiple faculty positions in the broad areas of chronobiology at Assistant
/Associate /Full Professor level are available in Soochow University Center
for
Circadian Clocks (SUCCC) in Suzhou, China.
We are seeking outstanding scholars who use fruit fly, zebrafish, mouse or
human to investigate molecular genetic mechanisms underlying circadian
regulation,
or study regulatory roles of circadian clocks in development, reproduction,
metabolism, sleep, behaviors, i... 阅读全帖 |
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l**********1
发帖数: 5204 | 22 it might take 3 Ys or even more conditional GPCRs (--) (+-) (++)
Mouse/Rat/zebrafish
LZ her/his CEO/CFO can not allow him to do that
so that kind of task always likes below:
同主题阅读：现在的核心问题是没有什么上手快，费少，钱又多，又稳定，又体面，又
不是青春饭的protocol/method/patent
[版面:生物学][首篇作者：KeeVan] , 2012年09月25日
http://www.mitbbs.com/article_t/Biology/31733655.html |
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l******g
发帖数: 2 | 24 Postdoc positions are available for highly motivated, independently thinking
individuals with strong interests in tool development and/or basic
molecular/cellular mechanisms.
Our lab is focused on: 1) developing unique tools; 2) applying these tools
to biological/biomedical study. Our study ranges from single-molecule to
whole-animal, including: 1) protein-protein interactions; 2) cell-cell
interactions; 3) tissue development and malfunction (e.g. tumor,
neurodegeneration).
We are looking for ca... 阅读全帖 |
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T*********l
发帖数: 298 | 25 Methods in Molecular Biology
2012, Volume 926, 317-329,
DOI: 10.1007/978-1-62703-002-1_20
Title:
Application of embryonic and adult zebrafish for nanotoxicity assessment.
email: g*******[email protected]
有包子！谢谢！ |
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C***2
发帖数: 62 | 26 Hey, I have read several articles using 2A to generate transgenic mouse,
please refer the following articles( I just copy the titles, it is very easy
to search fulltext if you are interesting):
1.High Cleavage Efficiency of a 2A Peptide Derived from
Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and
Mice
2.Correction of multi-gene deficiency in vivo using a
single ‘self-cleaving’ 2A peptide–based retroviral vector
3.Faithful Expression of Multiple Proteins via 2A-Peptide Self-Processing:... 阅读全帖 |
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c********r
发帖数: 1125 | 27
在zebrafish里面我听说会影响早期发育得。。 |
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Z******5
发帖数: 435 | 28 我们这边研究斑马鱼发育有人用。
就是把目的基因构建到带有T7或者SP6等启动子的质粒中，然后用体外转录系统转录出
mRNA，注射到zebrafish的卵里。 |
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l**********1
发帖数: 5204 | 29 炸药生理医学奖的2013 Y 提名 RAW 被取消了吧
from this one:
HTTP : //www.sciencedirect.com/science/article/pii/S1535610803000308
more pls refer
同主题阅读：Re: 大家说说Cancer cell metabolism吧，这个越来越火啊！
[版面:生物学][首篇作者：lostashoe] , 2012年11月02日
http://www.mitbbs.com/article_t1/Biology/31748337_0_3.html
>>
打脸的文章出来了
http://www.ncbi.nlm.nih.gov/pubmed/23267074
M2 isoform of pyruvate kinase is dispensable for tumor maintenance and
growth.
alfa-
---
>>
发信人: Zebrafishing (饕餮), 信区: Biology
标 题: Re: 大家说说Cancer cell metabolism吧，这... 阅读全帖 |
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S******1
发帖数: 6 | 30 PhD student position available
Department of Molecular Cell Biology, Leiden University Medical Center, The
Netherlands
Overview of the project and role
We are looking to recruit a PhD student to investigate the role of TGF-&#
61538; family members in liver fibrosis and cancer. A combination of
molecular biology, biochemistry, cell biology and animal model based
techniques will be used. The research project will be performed at the
Department of Molecular Cell Biology, Leiden University Medical ... 阅读全帖 |
|
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G*****m
发帖数: 198 | 32 in zebrafish, some cells will happen. lox-DsRed-lox-GFP will become lox-GFP.
don't know why. |
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P****d
发帖数: 564 | 33 I'm surprised by the numbers of R01s on basic research, so I did some search
at NIH RePORTER. I searched for active projects/R01 equivalents at NIMH,
NINDS, NIAAA and NIDA. Turned up about 400 projects with the key word
elegans, drosophila or zebrafish. Another 1,500 with mouse. Very
interesting sociology of basic research. I'm glad that you motivated me to
do the search!
I suspect that NIGMS (chemistry), NIBIB (imaging), NEI, NICHD will all
participate, perhaps a couple more ICs where the ... 阅读全帖 |
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y******8
发帖数: 1764 | 34 Here are what I did
NIH reporter,
key words: elegans, drosophila or zebrafish
Insitute: NINDS, NIMH
Application Type: New, or competing renewal
R01,
Active
return number is 29. So the average per insitute is less than 20 new R01 a
year.
Except bigbull DEISSEROTH with a grant of more than 5M a year, other costs
are routine numbers for R01.
search
to
obvious. |
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c****r
发帖数: 199 | 35 Cell Mol Life Sci. 2012 Sep;69(18):3069-77. doi: 10.1007/s00018-012-0985-6.
Epub 2012 Apr 19.
Left-right asymmetry in zebrafish.
Matsui T, Bessho Y.
Please send pdf to: a********[email protected]
Many thanks! |
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p*****c
发帖数: 20445 | 36 A-D E-K L-P R-Z
Peter Baumann, Ph.D.
HHMI Early Career Scientist
Stowers Institute for Medical Research
Peter Baumann’s research focuses on beginnings and endings: beginnings in
the form of reproduction in unisexual lizards and endings in the form of the
telomeres that protect chromosome tips.
Each time chromosomes are copied, telomeres—specialized stretches of DNA
that extend from the ends of each chromosome—become progressively shorter.
With each cell division, they ero... 阅读全帖 |
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a********k
发帖数: 2273 | 37 小牛吧，做zebrafish的，我觉得比不上schier |
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k******y
发帖数: 897 | 38 如题，求文章：
Adult zebrafish as a model for successful central nervous system
regeneration.
Becker CG, Becker T.
Restor Neurol Neurosci. 2008;26(2-3):71-80. Review.
非常感谢 |
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A********o
发帖数: 1307 | 39 Min-Kyeong Yeo,Misook Kang. Effects of Cu x TiO y nanometer particles on
biological toxicity during zebrafish embryogenesis. Korean Journal of
Chemical Engineering, May 2009, Volume 26, Issue 3, pp 711-718
h*****[email protected] Thanks |
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m******a
发帖数: 553 | 40 最近办绿卡急用，求各位同仁把不要的review推荐给我。以下是我的self
recommendation letter，我是做以斑马鱼为模式生物的心血管研究的，并对cancer和
其他分子生物学有研究。多谢！
My name is Jingchun Yang. I am currently working at Mayo Clinic College of
Medicine, USA, as a Research Associate. I got my Ph.D. degree from Institute
of Genetics, Fudan University, China in 2008. I would like to contribute as
a reviewer to review papers for xxx. I have published 9 papers in peer-
reviewed journal including FASEB Journal, Circulation Research and Human
Molecular Genetics. T... 阅读全帖 |
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n***m
发帖数: 292 | 41 Curr Med Chem. 2013 May 27. [Epub ahead of print]
Mitochondria Damage in the Pathogenesis of Diabetic Retinopathy and in the
Metabolic Memory Associated with its Continued Progression.
Kowluru RA.
J Vis Exp. 2013 Feb 28;(72):e50232. doi: 10.3791/50232.
A zebrafish model of diabetes mellitus and metabolic memory.
Intine RV, Olsen AS, Sarras MP Jr. |
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c********n
发帖数: 67 | 42 Neutrophil Motility In Vivo Using Zebrafish
Thanks,
h***[email protected] |
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l**********1
发帖数: 5204 | 43 cited,
>Both zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs)
>can be used to mutagenize genomes at specific loci, but
these systems require two different DNA binding proteins
flanking a sequence of interest, each with a C-terminal
FokI nuclease module. As a result these methods have not
been widely adopted by the plant research community.
from
>Belhaj K et al., (2013).
>Plant genome editing made easy: targeted mutagenesis in model and crop
plants using the CRISPR/Cas >system.
>P... 阅读全帖 |
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m******g
发帖数: 467 | 44 Science 14.04.04
推荐：
1. Designer Chromosome
Total Synthesis of a Functional Designer Eukaryotic Chromosome
稍微了解一下：
1. Many individual stories for scientists
2. Designer Embryo
Construction of a Vertebrate Embryo from Two Opposing Morphogen Gradients
Nodal and BMP in Zebrafish
小知识：
1. Engineering Larger DNA Structures
Polyhedra Self-Assembled from DNA Tripods and Characterized with 3D DNA-
PAINT |
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x******g
发帖数: 289 | 45 1.
Title: A polymer tandem solar cell with 10.6% power conversion efficiency
Author(s): You, Jingbi; Dou, Letian; Yoshimura, Ken; et al.
Source: NATURE COMMUNICATIONS Volume: 4 Article Number: 1446 DOI: 10.
1038/ncomms2411 Published: FEB 2013
Times Cited: 133 (from Web of Science)
Context Sensitive Links
[ View the abstractView abstract ]
2.
Title: Efficient genome editing in zebrafish using a CRISPR-Cas system
Author(s): Hwang, Woong Y.; Fu, Yanfang; Reyon, Deepak; et al.
Sourc... 阅读全帖 |
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J********e
发帖数: 341 | 46 For my friend, also for people who are looking for similar positions. Please
contact him directly.
Laboratory Research Positions in Cardiovascular Regenerative Biology (UCSF,
Mission Bay, San Francisco CA)
We are seeking highly motivated candidates with interests in regenerative
biology and medicine to join Dr. Huang’s laboratory at UCSF. The laboratory
has a general interest in vertebrate organ development, regeneration and
repair, and is currently focusing on heart regeneration using both zebr... 阅读全帖 |
|
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s****n
发帖数: 16 | 48 it is popular in flies and zebrafish. why not in mice? any reasons other
than convenience? |
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b******k
发帖数: 2321 | 49 本来想搭一个荧光显微镜加一个电动步进的平台，用来给96孔板照相，结果几家仪器公
司来推他们带成像功能的microplate reader，比如biotek的cytation3，MD的i3等等，
看照片的话似乎成像质量也不错了，不比一般的荧光显微镜差多少，而且这样的系统能
很好的配高通量系统，比如机器手和liquid handing system，价格也不贵，着实吸引
人。
不知道有没有用过类似系统的说说看到底怎么样？拍出来的照片有发表质量么？能替代
一般的荧光显微镜么？我们主要就是在96孔板里面养zebrafish larvae，看各种荧光标
记的变化，会做一些中小规模的化合物筛选。 |
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L****T
发帖数: 169 | 50 这些系统一般都是针对细胞的，96个孔的焦距基本差不多，只要在一个里面调整好了，
其余基本就不会差太多。价格应该是同等配置荧光显微镜的5倍左右。
Zebrafish可能自动对焦会很麻烦，中小通量可能没有你手动来的快。 |
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