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全部话题 - 话题: transduce
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x***h
发帖数: 9020
1
ABSTRACT
For modern systems, wear estimation plays an important role
in preventing failure, scheduling maintenance, and improving
utility. Wear estimation relies on a series of sensors, measuring the state
of the system. In some components, the sensors
used to estimate wear may not be fast enough to capture brief
transient states that are indicative of wear. For this reason
it is bene?cial to be capable of detecting and estimating the
extent of component wear using steady-state measurements.
Thi... 阅读全帖
x***h
发帖数: 9020
2
For modern systems, wear estimation plays an important role
in preventing failure, scheduling maintenance, and improving
utility. Wear estimation relies on a series of sensors, measuring the state
of the system. In some components, the sensors
used to estimate wear may not be fast enough to capture brief
transient states that are indicative of wear. For this reason
it is beneficial to be capable of detecting and estimating the
extent of component wear using steady-state measurements.
This ... 阅读全帖
N**W
发帖数: 65
3
想弄个kayak 钓鱼,看了很多装 fishfinder transducer 的 youtube videos。好像有
一个人是把 transducer 装在 kayak 仓内贴底的。想想好像应该也是可以的,
transducer 本身就是包在塑料/橡胶里的,多包一层应该不会阻断sonar 信号。不知道
版上的大神们有没有试过,或者这方面的信息。先谢一个。
l****k
发帖数: 3388
4
deeper fishfinder
[在 NXNW (北偏西) 的大作中提到:]
:想弄个kayak 钓鱼,看了很多装 fishfinder transducer 的 youtube videos。好像
有一个人是把 transducer 装在 kayak 仓内贴底的。想想好像应该也是可以的,
:transducer 本身就是包在塑料/橡胶里的,多包一层应该不会阻断sonar 信号。不知
道版上的大神们有没有试过,或者这方面的信息。先谢一个。
i*******n
发帖数: 3
5
来自主题: Faculty版 - Transducers 2011 Beijing?
Does anyone here know about the abstract/paper submission to Transducers
2011 in Beijing? The website site has nothing related to the format or
submission. I sent three emails to those contact emails. No response at all!
!!
t**********r
发帖数: 145
6
老板要我花一段时间做个过程优化, 已经有Lentivirus vector带目标基因来transduce
primary human T cells, 想提高transduction efficiency, 都有哪些过程参数, 如
何优化?
多谢多谢!!!
B**********y
发帖数: 261
7
钓鱼装备无极限啊!
1) Amazon上的这款有两种价格,其中一种是单机,不带transducer,那么是否带各类
的wiring cables?如果是带了transducer的,是不是肯定带有各类必须的cables?
不带transducer,应该只带电源线,没有其他各类的wiring cables
如果是带了transducer的,肯定带有各类必须的cables
如果要StructureScan®功能,需要另外买一个StructureScan® HD
transducer.
2) 懒得拖船到dealer那里,考虑自己安装,看了看,感觉不难,请教是否可以自己安
装?
可以,取决于您的DIY能力 :)
3) transducer的bracket是否可以用强力水下胶固定?省下钻孔的麻烦?
用强力水下胶固定?那要看胶力强度如何
建议用不锈钢螺丝固定
4) 不想用dashboard那一堆线,真搞不明白,是否可以自己引线,不接安全盒?怎么
拆甲板来引线?我翻了tracker手册,没找到怎么引线,也没找到怎么拆甲板?能否指
点一二?(Bass Tracker Pro Team... 阅读全帖
h*i
发帖数: 3446
8
来自主题: Programming版 - 王垠新文 为Java说句公道话
我不知道你说的transducer是不是同一个东西。不太可能是同一个东西。
我说的是这一系列事件:
当2014年Rich Hickey推出transducer的时候,
http://blog.cognitect.com/blog/2014/8/6/transducers-are-coming
他用了几个类似type signature的假代码例子来介绍概念,这就引发了一些haskell程
序员试图在haskell里面来实现transducer的活动,和一系列的有关能不能type
transducer的争论, Rich Hickey自己也参加了这个活动,比如:
http://conscientiousprogrammer.com/blog/2014/08/07/understandin
https://news.ycombinator.com/item?id=8342718
最后的结论是transducer的特性是原创的,比如early stopping, stateful,
completion operation, init等等,那种“其实transducer就是X“的说法都是错的。... 阅读全帖
W*******s
发帖数: 18705
9
很多人不知道,所以你不懂也正常。
Transducer内部构筑基本是通用很多不同的声纳,只要声纳内部的构造频率没有改变,
同一厂家的Transducer可以通用,比方说Humminbird的SideImage系列最低至最高的
1199都通用一个Transducer,新出的Onix/Ion,声纳构造不一样了,DI是独立的Built-
in,这就不能用在前期的声纳上。Trolling或是Transom Mount的声纳本质上一样的东
西,但是外部构造不一样,Transon船尾的声纳有个瓶颈弧度用螺丝固定,碰到障碍物
会翻起来不至于伤害Transducer,这个瓶颈弧度用在Trolling马达上就不行了,
Transducer要平面的才能妥妥的绑在TM上。Again,Retail Box里面是Transom Mount,
厂家免费给换Trolling Mount。
附图是我的老声纳的二个Transducer,一模一样的声纳,船尾跟船头的Transducer完全
不一样,船头那个是2D声纳Universal吧,可以通用很多不同的声纳了。图三不是我的
船,用原装船尾的声纳是错误的。
明白没?
KV
发帖数: 5728
10
非常感谢。俺对fishfinder一窍不通,今天上午看了看hummingbird和lowrance的
fishfinder,综合考虑了一下,以下这款感觉符合要求,大小也算合适,内置了GPS和
地形扫描,可以加一个无线传输。
Lowrance HDS 9 Gen2 Touch screen
再配一个无线传输器 Lowrance WIFI-1 Module,感觉这样可以省下船头的fishfinder
,直接同步在手机上看了。
我有几个问题,请教你和版上的各位懂Fishfinder/GPS的大侠,请帮忙看看。
1) Amazon上的这款有两种价格,其中一种是单机,不带transducer,那么是否带各类
的wiring cables?如果是带了transducer的,是不是肯定带有各类必须的cables?
2) 懒得拖船到dealer那里,考虑自己安装,看了看,感觉不难,请教是否可以自己安
装?
3) transducer的bracket是否可以用强力水下胶固定?省下钻孔的麻烦?
4) 不想用dashboard那一堆线,真搞不明白,是否可以自己引线,不接安全盒?怎么
拆甲板来引线?我翻了tr... 阅读全帖
KV
发帖数: 5728
11
综合考虑了一下,今天去west marine看了看实物,决定定7英寸的,否则太大了,
tracker的console那里太小。傍晚回来认真对比了Hummingbird 8 ci touch,Simrad
NSS7,还是觉得Lowrance HDS 7 gen 2 touch胜出很多,在定transducer时候很犹豫,
在50/200 hz和83/200 hz之间对比了很长时间,后来看网上资料,说100尺以内,83/
200略好,100尺以外,50/200更好。所以transducer定的是83/200。地形扫描的
transducer也一并定了,安装的位置还没研究,我考虑马达两侧一边一个transducer,
不知道是否可行。
还订了Lowrance WIFI module,这样在船头就可以看手机操控,省下一个fishfinder。
marker也顺便多定了几个,看到水下树枝就扔定位。过两天到了以后,先研究一下怎么
用,然后安装,不知道transducer这东西,dry run是否可行?必须要在水里么?
youtube上的同款,安装好wifi module以后,可以用手机,pad或者... 阅读全帖
k******9
发帖数: 878
12
来自主题: HiFi版 - 请大家帮忙推荐卡拉OK音响
如果准备买JBL ES80,那Onkyo TX-NR626可以搭配吗,我把参数都列在下面,麻烦高手
看看,谢谢了
JBL ES80. Specifications General Specifications Finish Black Series ES Wall-
Mount Capable No Type Floorstanding Audio Specifications Crossover
Frequencies 700Hz, 3.6kHz a?" 24dB/octave; 12kHz a?" 18dB/octave Maximum
Recommended Amplifier Power 200W Power Handling (Recommended) 100W
Sensitivity (2.83V@1m) 91dB Power Handling (Peak) 400W Nominal Impedance 8
ohms Frequency Response 45Hz a?" 40kHz Speaker Specifications High-Frequncy
Transduc... 阅读全帖
m********u
发帖数: 3942
13
来自主题: JobHunting版 - Acoustic Engineer in CA
Transducer speaker design experience is a must.
有兴趣的同学站内或者发邮件联系 [email protected]/* */
Job Summary
The Engineer in this position serves as the primary technical point of
contact and in-house expert for a wide range of products and acts as the
interface between the company and its customers. (S)he will guide the
audio designs and work with R&D, production, and other teams to introduce
new products, improve design performance, and solve acoustic related issues
arisen in production.
This j... 阅读全帖
w**s
发帖数: 367
14
来自主题: Fishing版 - 请教方法:怎样去掉epoxy?
今天 fishfinder transducer 从kayak壁上脱落了。
请教我怎样能去掉epoxy 从transducer 表面上?transducer 表面好像是比朔料坚硬的
某种材料。我粘transducer时,涂了比较厚的epoxy。
W*******s
发帖数: 18705
15
来自主题: Fishing版 - KV进来看看这个新玩意。
太牛鼻了,1.6英亩的覆盖面积。
Product Description
PRE-ORDER TODAY and get FREE SHIPPING! Estimated to ship August 1st, 2012
Revolutionary 360° Imaging scans underwater places that are virtually
impossible to see with conventional sonar. It produces a 150-ft. beam that
sweeps in every direction to create a 300-ft. circle of detection around
your boat, letting you see fish all around you without moving over them (1.6
acres of total coverage). The imaging beam sweeps the surrounding water and
detects fish, unde... 阅读全帖
k******9
发帖数: 878
16
来自主题: Music版 - 请大家帮忙推荐卡拉OK音响
这种卡拉OK要求的音响,有什么参数要求,这方面一窍不通,听大家说音响容易烧坏,
那是要哪个参数高或者低?有具体的选购参数标准吗?
我在版上翻了翻,有推荐JBL ES80的,还有BOSE301的,前几天看到NEWEGG上有POLK打
折的,等等等,大家可以给个建议吗?
另外如果选好了音响,如何看功放的参数和音响是否匹配,不会烧坏也不会推不上去,
那要看哪几个项目的数值,实在是什么都不懂,望高手帮忙!感激不尽
如果准备买JBL ES80,那Onkyo TX-NR626可以搭配吗,我把参数都列在下面,麻烦高手
看看,谢谢了
JBL ES80. Specifications General Specifications Finish Black Series ES Wall-
Mount Capable No Type Floorstanding Audio Specifications Crossover
Frequencies 700Hz, 3.6kHz a?" 24dB/octave; 12kHz a?" 18dB/octave Maximum
Recommended Amplifier... 阅读全帖
KV
发帖数: 5728
17
地形扫描transducer的个头真惊人,被彻底镇了,对比之下,Down scan/ Side scan的
transducer的个头看起来就像小弟弟,而扫描地形的简直就是一只大脚。
现在的问题是,是需要把这两支transducer并排安装,还是可以分开两侧安装。哪种比
较好?
另外,挂这么个大家伙在船屁股后面,实在难看。
KV
发帖数: 5728
18
来自主题: Fishing版 - 眼高手低- 半途总结
买了条淡水船一个月内,虽然属于打酱油人士,但也连续定了很多额外的设备,忙了一
阵子,后来一直忙,就没继续干,计划中的活主要有以下几点
1)引线,battery compartment到console,已完成
2)拆除原配fishfinder和transducer,已完成
3)安装升级后的fishfiner和transducer以及structural scan transducer,完成了一半
4)电池升级为军用agm电池,安装minn kota随船充电器,完成
5)拆除原配trolling motor,未完成
6)升级线,从battery compartment到bow,未完成
7)安装升级trolling motor,未完成
8)安装自动升降trolling motor设备,未完成
9)安装船上无线网络系统,连接pad和手机,操作fishfinder/gps,未完成
10)安装power pole,未完成
这个周末很累,今天没去上班,睡了懒觉,下午在家忙着升级电池,原配电池如图一,
两块,简洁明了的接线,在拆除过程中,把一个电极的螺帽拧折了,没办法只好剪掉重
新接,浪费了一个多小时... 阅读全帖
l*****m
发帖数: 122
19
来自主题: Biology版 - 问个病毒侵染BMDC的问题
You're absolutely right...I've seen tons of papers using either lenti- or
retro-virus to transduce BMDCs. Although the protocols vary a little bit
from one to another, none of them worked for me so far :(
I don't see much cell death by PI staining, but the pattern of the side/
forward scatter of the transduced cells does look a little different from
that of the untransduced cells (gated on live cells only). And more
importantly, CD11c expression is highly inhibited in transduced cells.
One exper... 阅读全帖
f**d
发帖数: 768
20
来自主题: Neuroscience版 - eBook: From computer to brain
这是一本计算神经科学的优秀著作,全文拷贝这里(图和公式缺),有兴趣的同学可以
阅读
如需要,我可以分享PDF文件(--仅供个人学习,无商业用途)
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖
w*******y
发帖数: 60932
21
MartinLogan ESL Series Purity DarkCherry Fully Powered Hybrid Electrostatic
Loudspeaker Each:
http://www.newegg.com/Product/Product.aspx?Item=N82E16882981016
$200 off w/ promo code EMCKCJE74, ends 7/17
Model
Brand
MartinLogan
Series
ESL Series
Model
Purity DarkCherry
Spec
Type
Floorstanding Speakers
Sold As
Each
Driver Units
High Frequency Transducer: XStat CLS electrostatic transducer
- Panel Dimensions: 28" 8.6" (71.1 21.8cm)
- Radiating Area: 241 in2 (1,550 cm2... 阅读全帖
w*******y
发帖数: 60932
22
Amazon Marketplace has Harman Kardon Soundsticks III:
http://www.amazon.com/Harman-Kardon-Soundsticks-III/dp/B0042F3K
fulfilled by Amazon for $116.23.
Next lowest price from a reputable seller is $135. These sell new for $169
@ most places.
Listen to a work of art.
Praised for its stunning design, the harman kardonSoundSticks II speakers
and subwoofer system became a milestone in harman kardon history when it
became part of the permanent collection at New York City's Museum of Modern
Art (MoM... 阅读全帖
N***r
发帖数: 2539
23
i don't know exactly what kind of instruments they are using in a NPP (
nuclear power plant).
I'd say probably digital kind of, probably pressure transducer so you can
read digital numbers.
I know in my lab, we use both pressure gauge and pressure transducer.
pressure gauge sometimes is really confusing, see a pic below, they do have
different units there. of course, as a well trained engineer you should not
make mistake like reading an incorrent unit. well, in an accident like this,
you can't g... 阅读全帖
s***a
发帖数: 4921
24
JBL Venue Series Monitor 5-Inch 2-Way Speakers (Pair) $107.69 Shipped
http://www.fatwallet.com/t/18/652943/
This is about $70 less then most places are selling these.
* Maximum Recommended Amplifier: Power 125 Watts
* Handling: (Continuous/Peak) 60/240 Watts
* Impedance: 8 Ohms nominal
* Sensitivity: (2.83V/1m) 86dB
* Frequency Response: (-3dB) 68Hz - 20kHz
* Crossover Frequency: 3000Hz
* High-Frequency Transducer: 3/4 inch (19mm) Titanium-laminate dome,
shielded
* Low-Frequency Transducer: 5 in
g*****9
发帖数: 4125
25
A few speaker180 degree out of phase.
If you want mount on the fence, you can use tactile transducer, same 180
degree out of phase. It might require some testing to perfect this. That is
what I use to cancel the sound from my home theater room. I have over 3000
watts (one 400 watt amp for each channel) for the theater, I used about 20
tactile transducers mounted strategically in between the drywalls to block
the sound, after I spend some time tuning it, it works amazingly well now.
L******r
发帖数: 141
26
来自主题: Immigration版 - 怎样写好推荐信 EB1A/B
All the materials were taken from Administrative Decisions (Aliens with Extraordinary Ability/ Outstanding Professors and Researchers). 所以这些话是权威!!!
I downloaded All the AAO decisions for the past three years. I read one by one for a total number of more than 200 decisions. I took good paragraphs from recommendation letters which IOs thought have met criterion of contribution. Because the works were from PDF files, I had to reformat them into word file.
It took me many hours to do the work and I... 阅读全帖
s*******e
发帖数: 740
27
来自主题: PHILADELPHIA版 - 费城startup工作机会 (转载)
【 以下文字转载自 Biology 讨论区 】
发信人: smallbite (一小口), 信区: Biology
标 题: 费城startup工作机会
发信站: BBS 未名空间站 (Sat Feb 3 16:45:54 2018, 美东)
Scientist/Associate Scientist of immunology
Responsibilities:
Primary immune cell isolation and culture
Antibody purification and characterization
DNA vector construction and cell transfection
Retrovirus production and cell transducing
Skills/Requirements
Experience in primary immune cell culture, immune cell-based assays, flow
cytometry, and cell sorting
Experience in retr... 阅读全帖
A*********r
发帖数: 2115
28
I have the Hawkeye F335P model.
It is surprisingly accurate but it is not very feasible to use from shore.
The cord sinks and retracts the transducer towards shore.
It is good for small boat/Kayak/Canoe fishing and ice fishing.
The transducer can listen through the hull or ice layer.
Bottom line, it will find you fish.
m********n
发帖数: 2995
29
来自主题: Fishing版 - 请教探鱼器fish finder
transducer本身有声音是完全正常的,这个声音就是声纳的脉冲啊,这个声音出去,回
来的回波被接收到才有你的图像。模拟的时候transducer不工作,所以没有声音。
m********n
发帖数: 2995
30
来自主题: Fishing版 - 请教探鱼器fish finder
根据网上的讨论来看,老的humminbird,Lowrance很多型号都有。新的可能没有,也可
能是没有注意。这个tick是很轻很轻的从transducer里面出来的声音是从sonar屏幕出
来的声音。船长的船如果transducer是装在船底里面的,估计比较不容易听到。只有在
比较安静的情况下,sonar工作的时候才会有这个声音。
s****s
发帖数: 486
31
来自主题: Fishing版 - 【秋风送爽】跟着饵鱼走
我主要在小河里钓。找creek turns 或者 shallow flats。transducer是humminbird
XTH 9 20 P plastic thru hull. 我的是ocean kayak trident,有一个专门给
transducer的scupper hole,直接安在那儿。
h******1
发帖数: 1930
32
KV老大问的问题都太高深了,我真的不算懂,不敢瞎说。
就目前的评价和普及率来说, Lawrence的确是比hummingbird好。我们当初买
hummingbird主要是考虑便宜,一个package就行不用额外买transducer.
另外前两天船长不是推荐了Garmin么?我最喜欢的钓手Scott Martin就是用Garmin的,
似乎也没有什么问题。这个只要功能到位了,主要是习惯问题了吧。
船前面安装一台的好处大大的,装在trolling motor上,才能真正做到指哪儿打哪儿,
精准到厘米。否则在船后面transducer给你的信号,掉个头之后多少都会偏离。我觉得
前面的简单点就行, 不用啥gps,直接是个sonar,有down image最好。
这个就够了:
http://www.tacklewarehouse.com/Lowrance_Elite-5x_HDI_Color_Fish

fishfinder
y***n
发帖数: 2806
33
来自主题: Fishing版 - 在seaeagle 上装fishfinder
同求。我装transducer时不知道怎么固定比较好。看视频,有用橡皮泥的,有用胶加上
海绵垫的,我kayak运输时一般都是倒扣在车顶,老感觉这么搞好像不太结实,有没好
点建议?
fishfinder往kayak上装youtube上有视频的,自己要有电钻就行。但视频里装的
transducer方法,我感觉不太适合我。我还没法往船底打洞加螺丝固定,看着好像只能
用胶。
f**m
发帖数: 16
34
来自主题: Fishing版 - 在seaeagle 上装fishfinder
用硅胶把transducer粘在kayak里面的底部。在transducer下一定要没有气泡。
KV
发帖数: 5728
35
来自主题: Fishing版 - 淡水tracker pro team 175 txw升级
冬季版上真冷清,几天没来,十分钟看完所有帖子,南方的同志们在干嘛?
去年十一月底买条淡水船以后,开始了一步步地升级,此中得到版上同志们的大力帮忙
,学到了很多知识,重要的部分俺没有交给dealer去做(因为船的基本知识贫乏而不得
不自己干),大到型号小到镙丝俺都一点点研究和学习,这几个月受益匪浅,韩少,
boatmotorguy,pplulu等等几位大佬知识非常丰富,还有很多其他各位老大回帖,俺也
学了很多。
除非购买ranger,nitro等top of the line的淡水船,tracker这种船是很简单的入门
级,船上除了汽油马达外,其他大多主要配件都需要更换或者升级。我的教训是,最好
是购买之前研究明白,然后开单子让dealer去干,否则自己干的工作量太大了,而且开
销相当不菲,不划算,好处是能学到很多基本知识,对船的了解也更多。
我购买时候请dealer升级了自动锚等一些非关键配件,也根据新泽西的规定,downsize
了马达,但是忽略了关键的两样,即fishfinder/GPS和trolling motor,走了很多弯路
,买了一堆没用的配件,包括lifeline电池,现... 阅读全帖
e***d
发帖数: 288
36

不对吧, Transducer就一种吧.
放在TM上的是有配件, 绑上去的.
还没发现有专门真对TM的transducer
p****i
发帖数: 5597
37
来自主题: PhotoGear版 - 请问版上有EE的吗?
有个问题需要请教:
俺是纯外行,不过现在的工作需要用到超声,基本就是用FG产生信号,amplifier放大
,然后接到transducer。FG是一个EE的博士生设计的,amplifier是ENI 2100L,50db,
2手的
现在问题是:FG的信号设定是1V,1.5Mkz. Amplifier输出的应该的是316V,但是我观
测到的只有3V,如果amplifier的输出不连接任何cable,输出可以到67V,不过还是远
小于正常值。我同事已经用oscilloscope测出FG产生的信号正常。难道是amplifier的
问题?如果是的话如何检测呢?我们的这个transducer设计的是在1.5Mkz工作。
多谢大家的帮忙。
Y**u
发帖数: 5466
38
☆─────────────────────────────────────☆
wuyu (wuyu) 于 (Fri Sep 9 12:23:52 2011, 美东) 提到:
行禅在念住修行中有很重要的地位。跟大多数这儿的同学们一样,我没有福气有专门时
间在一个清静的地方做行禅。只能在上班路上做行禅,虽然效果不是很好,但千里之行
,始于脚下,希望为将来有机会密集禅修打下基础。
我曾经试着给每一步脚掌的移动分解标识“按,移,推,提”。开始感觉效果不错
,但缺点是标识经常跟不上所缘变化,导致标识变成了念口诀。后来请教卡玛兰蒂禅师
,她建议我只用察觉“左脚,右脚”。用这个方法我基本上能跟上所缘变化。但是很容
易走神被杂念打乱。到最后,我想了一个办法。这个办法跟我练习安般息念的经验有关
,我一般用数息(吸呼1-10)来使自己沉淀杂念。在行禅中,我也试着以“数步”让自
己能持续专注。我发现数七步是最好的:(一)容易持续专注;跟数息的道理一样。(
二)可进可退;如果环境嘈杂,或专注力弱,可只觉知“一三五七”步。如果专注力强
,可以“一二三四五六七”左右脚全部觉知(三)易于平衡;因为是奇... 阅读全帖
h*i
发帖数: 3446
39
来自主题: Programming版 - FP更接近人的思维
粗粗看上去有点像Clojure里面的transducer,可能transducer是Arrows的一种特例?
比较高深哦,等我退休了来研究吧。category theory, quantum computing, 还有啥
geometric algebra, 都是高大上的,我研究了一下没有入门,决定放入我的退休研究项
目单的东东。呵呵。
p*******n
发帖数: 6
40
来自主题: Biology版 - 请教phage transduction相关问题
我刚开始做金葡菌的致病因子,要用phage从一种strain上载入一个mutant DNA片段导
入另一个strain。用的phage是transducing phage,据提供者说high frequency of
lysogenization。我用plaques提取lytic phage,然后和recipient混合,incubate 20
分钟以后立即稀释、涂板,结果没有一个带resistant marker的colony长出来。
我的实验方法可能会出什么问题?
这种phage是不是主要进行specialized transduction?收集lysogen然后transduce是
不是可行的方法?
谢谢各位。
m**********d
发帖数: 137
41
之前做过很多lentivirus,都是超速离心virus之后infect细胞,transduction效率都
还不错,这次偷懒没离心,直接用的含有病毒的medium,可是puromycin select一两天
后细胞全死光了,以下是详细的protocol:
vector用的是pBABE-Luc (基于pBABE-puro,只不过也表达luciferase)
293T细胞,养在75cm2 flask里,用lipofectamine2000转染的,包装病毒用的是second
generation packaging system (PAX2,pMD2.G)
分别于转染后48hr和72hr collect medium,过了一下0.45uM的filter,又加了8ug/ml
的polybrene,就加到要transduce的细胞里去了,24hr之后开始puromycin selection
我能想到的问题可能是准备transduce的细胞有点太confluent了,但这也没法解释全死
光的结果,我想很可能是没有病毒包装,高人们帮忙看看这过程中可能是么出了问题
r**o
发帖数: 212
42
FASEB J. 2012 Aug;26(8):3140-7. doi: 10.1096/fj.11-198515. Epub 2012 Apr 24.
MicroRNA-31 targets FIH-1 to positively regulate corneal epithelial glycogen
metabolism.
Peng H, Hamanaka RB, Katsnelson J, Hao LL, Yang W, Chandel NS, Lavker RM.
Corneal epithelium relies on abundant glycogen stores as its primary energy
source. MicroRNA-31 (miR-31), a corneal epithelial-preferred miRNA,
negatively regulates factor inhibiting hypoxia-inducible factor-1 (FIH-1).
Since HIF-1α is involved in anaerobic ene... 阅读全帖
s*******e
发帖数: 740
43
来自主题: Biology版 - 公司成立不久,还不能办签证
Scientist/Associate Scientist of immunology
Responsibilities:
Primary immune cell isolation and culture
Antibody purification and characterization
DNA vector construction and cell transfection
Retrovirus production and cell transducing
Skills/Requirements
Experience in primary immune cell culture, immune cell-based assays, flow
cytometry, and cell sorting
Experience in retrovirus preparation and cell transducing
Experience in protein purification from yeast, E.coli and mammalian cells
Experience... 阅读全帖
m*******o
发帖数: 108
44
Gold Nanocages as Photothermal Transducers for Cancer Treatment
Journal: SMALL
Vol/Issue: 6 (7), Date: 2010, Pages: 811-817
Article: Gold Nanocages as Photothermal Transducers for Cancer Treatment
Author(s): Chen, J; Glaus, C; Laforest, R; Zhang, Q; Yang, M; Gidding, M
请发到m********[email protected]或站内都行
多谢!
o*****n
发帖数: 7
t******m
发帖数: 4
46
电路好象不是很难,关键是电流的测量,朋友告诉我用LEM的current transducer, 但我
觉得好象精度不够.比如我要测的SiC JFET, 开关时间在几十个纳秒,但是transducer反
应的时间要一个毫秒左右,waveform测出来应该误差很大吧...
本人绝对菜鸟,望达人不吝赐教.
d*****l
发帖数: 8441
47
涉及transducer吗?
Transducer更多是物理和电子电器
p****i
发帖数: 5597
48
俺是纯外行,不过现在的工作需要用到超声,基本就是用FG产生信号,amplifier放大
,然后接到transducer。FG是一个EE的博士生设计的,amplifier是ENI 2100L,50db,
2手的
现在问题是:FG的信号设定是1V,1.5Mhz. Amplifier输出的应该的是316V,但是我观
测到的只有3V,如果amplifier的输出不连接任何cable,输出可以到67V,不过还是远
小于正常值。我同事已经用oscilloscope测出FG产生的信号正常。难道是amplifier的
问题?如果是的话如何检测呢?我们的这个transducer设计的是在1.5Mhz工作。
多谢大家的帮忙。
d*****m
发帖数: 1
49
来自主题: Macromolecules版 - GPC help
I am working on an old GPC, Waters Multisolvent delivery 600E.
The pressure is fluctuating too much, and flow rate is not normal too.
If eliminate the solvent influence and pump prolem, could it be the problem of
the Transducer? I try to replace it, but the transducer is comparatively
expensive and my boss doesn't want to do it.
Can anyone help me, I am almost sick of that. Thank You!
d****y
发帖数: 2180
50
来自主题: MedicalCareer版 - CK form 1 block 2 Q18
Good point. Thanks.
I think the diagnosis of Cardiac Tamponade can be made even without Echo in
this case. Cardiac Tamponade is a clinical diagnosis.
This patient is hemodynamically unstable, isn't it urgent to tap some water
out to relieve the pressure to heart before any diagnostic test?
Of caourse the term paracentesis is not a good one, if this option
change to pericardiocentesis, which one would you choose?
http://emedicine.medscape.com/article/152083-treatment
Removal of pericardial fluid ... 阅读全帖
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