w***1
发帖数: 1951 | 1 赞一个，并握个抓！
我也是交通口的GIS开发，曾经和楼主用的差不多了。
不过现在工作了，反而用比较多的现成商业工具。
1. GeoDatabase: SQL Server 2008/2012 Geometry
2. Backend: .Net WCF MVC -> Visual Studio 2010/2012
3. GIS Services： ESRI ArcGIS Server
4. Frontend: ESRI Javascript API -> Sublime Text 2/VIM
5. Python Script: Notepad++/Sublime Text 2 自己配插件调试
6. Project Management: Visual Studio TFS+Git |
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c****e
发帖数: 1453 | 2 不可以，所以才是优点。JIRA就是狗屁各种数据库表的排列组合。每次checkin还要我
说用了多少时间, 美其名曰 smart comment. 在agile的开发环境下，就是自找麻烦。
对于老年产品track bug什么的还凑合。一星期一次production rollout,时间光是伺候
JIRA都不够，和微软的TFS一个德行。 |
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T******7
发帖数: 1419 | 4 yours link's content is too old.
Lot of things have changed.
5 years after that ppt.
just say one thing, they have changed to use ext4 not ext3. |
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N*n
发帖数: 456 | 5 差不多4-5年老。。因为ppt里提到09年的实现。。
从ext3 到 ext4 的差别，
comparing ext3 and ext4:
"Supports huge individual file size and overall file system size.
Maximum individual file size can be from 16 GB to 16 TB
Overall maximum ext4 file system size is 1 EB (exabyte). 1 EB = 1024 PB (
petabyte). 1 PB = 1024 TB (terabyte).
Directory can contain a maximum of 64,000 subdirectories (as opposed to 32,
000 in ext3)
You can also mount an existing ext3 fs as ext4 fs (without having to upgrade
it).
Several other new feature... 阅读全帖 |
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N*n
发帖数: 456 | 6 lots of things changed? like what else? |
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T******7
发帖数: 1419 | 7 when did they start double 11,
and when did they start "Miao-Shao" (seconds-kill)
also,
ext3 and ext4 are so different.
this diff-list you found is too superficial.
for example, one feature Taobao uses a lot from ext4 is inline data. Which
is developed by Taobao developers and merged to mainline tree already.
upgrade |
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N*n
发帖数: 456 | 8 看不太懂前两句。
淘光节是2010 11/11 开始的。
秒杀不清楚何时开始。
in-line data.简单查了一下
Inline Data
The inline data feature was designed to handle the case that a file's data
is so tiny that it readily fits inside the inode, which (theoretically)
reduces disk block consumption and reduces seeks. If the file is smaller
than 60 bytes, then the data are stored inline in inode.i_block. If the rest
of the file would fit inside the extended attribute space, then it might be
found as an extended attribute "system.data" within the... 阅读全帖 |
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z****e
发帖数: 54598 | 9 国内银行保险民航电信物流石油这些领域
构架基本上都是这几个外企在搞
在天朝参加这些外企组织的seminar
案例就喜欢拿大国企来说事，甚至还有殡仪馆医院，无处不在
后来的政府，搞电子政务，不过这块一般是金蝶什么在做
天朝政府还是不能相信外企
当时先吹web2.0，然后是soa，一路下来
稍微跟传统有点不同的就是淘宝到后期开始投入big data也就是tfs那些东西
就有点像amazon的那些东西
这个也是最近这些年发展起来的东西
这点比天朝官僚机构要强 |
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c****e
发帖数: 1453 | 10 来自主题: Programming版 - Jira Over complicated and over managed, even worse than TFS. we use Trello board
to manage all our
work items now. |
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g*****g
发帖数: 34805 | 11 来自主题: Programming版 - Jira Way better than TFS unless you work for M$
board |
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d***n
发帖数: 832 | 12 现在什么都往云里挪
什么都做成service
是什么语言还有那么重要么
需要什么就用什么
怎么快就怎么弄
我自己做的东西
一部分用vs和c#不妨碍我用
lucene和elastic search, Redis Cache
也不妨碍我用sendgrid之类的服务
数据库可以用sql azure, mangodb, azure storage。。。
.NET, Java, PHP, Node.js, Python都可以host在azure websites里
也可以从Git, TFS, GitHub deploy
总之，云平台可以聊的东西太多了
我做的小东东所有都在云里 |
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k**n
发帖数: 3989 | 13 是楼主快猛糙习惯了，从不适应其他SDLC吧。
不管java，c#都要build然后deploy,
现在连node.js都用上build了。
简单的说，
首先，你用vs在本机fix bug。。如用上TDD，mstest就能把大部分bug搞定，
然后你checkin TFS 或其他的source control， 然后continue Integration自动build
与deploy到服务器上。
服务器应该有dev,qa,uat ，Prod等，
你上QA后，所以测试由专门QAtester来完成。每个bug会记录在系统发给你修改。
稍正规点的软件公司都这样的。 |
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p**r
发帖数: 5853 | 14 我大牛个屁，就一斧头帮的混日子的。
这葵花板块至少一半码农的技术能甩我几条街。
我只是好管闲事，不管自己水平如何，都喜欢给人出出主意，顺便刷脸装个逼
很惭愧的说，之前一直在用c#，啥东西都放tfs
至于github，空有一个账号 |
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N********n
发帖数: 8363 | 15
identity啥的都是说辞。其实就是FP/immutable没那么神。race condition
导致处理mutable问题很复杂。immutable号称可以简化编程。那好，OOP靠边
站，请FP露一手吧。用immutable方式操作银行账户，把原地操作换成FP指定
的copy/append，楼上折腾半天结果连基本的存储操作都搞错。mutable环境下
头疼的RC问题换immutable照样没招，那些copy/append都是无用功。
至于保存immutable数据做audit，那不就是版本控制么。那是软件系统需求，
跟程序语言有啥关系？常用的github / svm / tfs / cvs / perforce 就是
典型的immutable存储。需要的话OOP照样写一个，又不是FP独有的概念。
世界上不是一切都是mutable，但也不是一切都immutable。你开车去加油站，
你的油箱是变成新油箱了，还是就在原箱加满油？你的车轮每转一圈就被复制
成新轮子了，还是原来的老轮子？凡事量身而做，把immutable当成真理然后去
生搬硬套，那是书呆子本末倒置闹笑话。 |
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g****t
发帖数: 31659 | 16 所有的数学书，物理书里面的map 都是不改
变量的，函数内也没有状态一说，immutable的思路不能轻视。
从实践来讲。我多年前写的有的code因为是reference transpancy,避免过一次编译器
重大事故。我并不知道什么是fp.
所以我个人看法，凡是明确描述数学物理模型的
代码，尽量和数学定义的函数一致。这样避免自造轮子。
如果需要算法
变种，用别的办法lift up到高一层，不要两种函数混一起。
: identity啥的都是说辞。其实就是FP/immutable没那么神。race
condition
: 导致处理mutable问题很复杂。immutable号称可以简化编程。那好，OOP
靠边
: 站，请FP露一手吧。用immutable方式操作银行账户，把原地操作换成FP
指定
: 的copy/append，楼上折腾半天结果连基本的存储操作都搞错。mutable环
境下
: 头疼的RC问题换immutable照样没招，那些copy/append都是无用功。
: 至于保存immutable数据做audit，那不就是版本控... 阅读全帖 |
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k******u
发帖数: 3137 | 17 我在xp的帮助中搜了一下"压缩",给你copy一部分:
压缩 NTFS 驱动器上的文件或文件夹
打开 我的电脑。
双击驱动器或文件夹。
右键单击要压缩的文件或文件夹，然后单击“属性”。
在“常规”选项卡上，单击“高级”。
选中“压缩内容以便节省磁盘空间”复选框，然后单击“确定”。
在“属性”对话框中，单击“确定”。
在“确认属性更改”中选择需要的选项。
注意
要打开“我的电脑”，请单击“开始”，然后单击“我的电脑”。
可以使用 NTFS 压缩已格式化为 NTFS 驱动器上的文件和文件夹。如果没有出现“高级”
按钮，说明所选的文件或文件夹不在 NTFS 驱动器上。
如果将文件移动或复制到已压缩的文件夹中，文件将被自动压缩。如果将文件从不同的 N
TFS 驱动器移动到已压缩的文件夹中，文件也将被压缩。但是，如果将文件从同一个 NTF
S 驱动器移动到已压缩的文件夹中，则文件将保留原始状态（或者是压缩的，或者是未压
缩的）。
使用 NTFS 压缩的文件和文件夹不能被加密。
可以选择将 NTFS 压缩的文件显示为不同的颜色。详细信息，请单击“相关主题”。 |
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r****q
发帖数: 22 | 18 ‘RB1 is related to RB’ is a statement, not a prediction or inference. You
may develop this into a problem to solve, but it is nothing more than a
meaningless or trivial one. I don’t see any comparability between solving
this problem and gene set analysis.
I never say experiment evidence is not needed. I just say it was not needed
to do our own verification experiment there. GAGE identified so many
pathways that GSEA and PAGE did not, and whose biological relevance has been
well established in li... 阅读全帖 |
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M*******s
发帖数: 108 | 19 there are transcriptome data
which TF will transcribe which gene, and also combinatorial effects of TF.
you can just use that kind of data to pull out your genes then you may get
TFs
but I doubt it is convincing enough for publish if without ChIP data
you may carry some further study. For instance, build up the regulatory
network first, then find the dysregulated genes from upstream to downstream
in the network, then you may find some trace of the dysregulation. Generally
, upper-stream usually ... 阅读全帖 |
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g*********3
发帖数: 177 | 20 这个有人做了。。。效果不是很好貌似。（转的质粒）
关键是你还得保证用于纯化目的的那段序列不会起到recruit TFs and GTFs...造成假
阳性 |
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h******y
发帖数: 351 | 21 You are absolutely right about the ground-breaking contributions of Yamanka'
s to the iPSC. Thomson deserves credit for the first to isolate human ESC,
but his contributions in making human iPSC was not as great as Yamanaka.
Shinya is humble and kept telling the audience the story that it was his
postdoc's (Kazutoshi Takahashi) original idea to transduce all 20 factors
together to see if his hypothesis would work, which anyone would think to be
a "stupid idea". Alas, it worked and they were the... 阅读全帖 |
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a****d
发帖数: 1919 | 22 iPS更应该与PCR比较，都是非常有应用价值的技术上的创新。PCR已经成为routin的实
验手段，iPS对于建立疾病模型进行药物筛选来讲，也是一样。当然iPS为大家打开一扇
窗，至少很多人不再认为用几个TFs来reprogramming是天方夜谭了。 |
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j*p
发帖数: 411 | 23 1. UCSC genome browser has many ChIP-seq tracks including TFs, Pol2 and
Histone modifications (H3K4me3, H3K27me3, H3K36me3, etc) on different cell
lines(through Encode/Gencode project). You can just active these tracks and
compare with what you have to see if your H3K4me3 data looks normal or not.
2. I suggest you normalize your ChIP-seq signals. For example, your normal
tissue ChIP-seq has total number mappable reads = 10M, your tumor sample has
20M mappable reads, then divide any signal from t... 阅读全帖 |
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s******s
发帖数: 13035 | 24 你要知道，BAC里面就是你要的基因 + 调控序列 - epigenetic markers
虽然很多被epigentically repressed的基因很可能表达，但是你缺少必要
的TFs的话就没有办法了。
?
is |
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P****o
发帖数: 172 | 25 We first fix GFP with 2% formaldehyde, followed by transcription factor
staining. This works for a bunch of TFs at our hands, but not sure whether
it will work for your case. |
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i*********0
发帖数: 915 | 26 What happens if ES cell oct4 gene is silenced by shRNA? Directly go for
differentiation or just lose the ES cell identity? Do I need to apply some
news TFs to achieve a differentiation after OCt4 knockdown? |
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l**********1
发帖数: 5204 | 27 要上 R package
pls refer:
van Hijum SA, Medema MH, Kuipers OP. (2009)
Mechanisms and evolution of control logic in prokaryotic transcriptional
regulation.
Microbiol Mol Biol Rev. 73: 481-509
Abstract
A major part of organismal complexity and versatility of prokaryotes resides
in their ability to fine-tune gene expression to adequately respond to
internal and external stimuli. Evolution has been very innovative in
creating intricate mechanisms by which different regulatory signals operate
and inter... 阅读全帖 |
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H****N
发帖数: 997 | 28 It does not matter which strand the primer is from. The methylaton on either
strand can always be detected. Most TFs binds to both strand of DNA, but
there are exceptions. |
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p****y
发帖数: 13 | 29 Field: transcription factors (TFs), transcriptional regulation, RNA binding
proteins (RBPs), Posttranscriptional regulation
Experience: reviewer for 3 Journal |
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s*****r
发帖数: 477 | 30 Is there any database that provides the list of transcription factor respons
ive genes, for example, includes a list of genes that are regulated by NFkB
or other TFs? Thanks. |
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K**R
发帖数: 193 | 31 主要第一是看看你测试什么的binding， 比如转录因子TFs 还是histone的。
设计primer 比较麻烦，
首先看promoter位置，看有人报道过没有。 如果没有就预测，
主要是具体选择哪个区域，有时候不一定严格选promoter， promoter附近也可以。
根据你要pull down 蛋白来定
看看Dr young 的设计方法，希望你能用的上。
http://younglab.wi.mit.edu/regulatory_code/ |
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b******s
发帖数: 1089 | 32 chip-seq不是找下游targets吗？
我现在想找上游的binding TFs |
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l**********1
发帖数: 5204 | 33 pls refer fresh new both papers:
a,
by Morozov VY and Ioshikhes IP. (2013).
Optimized Position Weight Matrices in Prediction of Novel Putative Binding
Sites for Transcription Factors in the Drosophila melanogaster Genome.
PLoS One. 8: e68712.
Abstract
Position weight matrices (PWMs) have become a tool of choice for the
identification of transcription factor binding sites in DNA sequences.
below ignored
In the present study, we extended this technique originally tested on
single examples of trans... 阅读全帖 |
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m******g
发帖数: 467 | 34 今天去找导师汇报，本来觉得自己的内容太少，会被批评的，结果导师还鼓励了我，我
一定要加倍努力呀！感动ing。希望以后也能成为我导师那样的好导师！
Science 13.12.13
推荐：
1. Exonic Transcription Factor Binding Directs Codon Choice and Affects
Protein Evolution
概要：Long-Term Dynamics of Adaptation in Asexual Populations
Mean fitness seems to have no upper bound
Power law better than hyperbolic model
2. The Hidden Codes That Shape Protein Evolution
TF binding code
Human genome in 81 cell types
Constraining codes
~14% codons within 86.9% of genes are occupied by TFs
3. Cave... 阅读全帖 |
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m******g
发帖数: 467 | 35 今天去找导师汇报，本来觉得自己的内容太少，会被批评的，结果导师还鼓励了我，我
一定要加倍努力呀！感动ing。希望以后也能成为我导师那样的好导师！
Science 13.12.13
推荐：
1. Exonic Transcription Factor Binding Directs Codon Choice and Affects
Protein Evolution
概要：Long-Term Dynamics of Adaptation in Asexual Populations
Mean fitness seems to have no upper bound
Power law better than hyperbolic model
2. The Hidden Codes That Shape Protein Evolution
TF binding code
Human genome in 81 cell types
Constraining codes
~14% codons within 86.9% of genes are occupied by TFs
3. Cave... 阅读全帖 |
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m******5
发帖数: 1383 | 36 只是想挖几个现有的发表过的Chip-Seq 看几个TFs对GOI的调控
请问新手上路用哪个broswer好？
galaxy怎么样？ |
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p****1
发帖数: 275 | 39 I am a .NET/SQL Programmer, about 6 years industry experience, expertise
in ASP.NET, VB.NET, C#, MVC, SQL, SSRS, got used with TFS / Share Point
environment. I consider myself solid middle to senior level developer,
technical person, lacking leading experience. H1B visa sponsorship is
needed, my H1B visa can be renewed as needed. I can relocate.
Should you have any information, please contact me. Thanks, |
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e**o
发帖数: 5509 | 40 来自主题: _BayAreaFishing版 - 鱼竿Deal 刚下单。我老也快成有两个杆的人了。
TFS-SW/SA Spinning 230/15 5.2:1 7' Saltwater 12-
30 1 to 3 |
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N***i
发帖数: 2063 | 41 Best-Rated Financial Stocks Updated June 23, 2010
http://www.nasd100.com/2010/06/bestrated-financial-stocks-updated-june-23-2010.html
Ranking | Company (Ticker) | Average Rating Value | Average Rating
1 IRSA Inversiones Representaciones (ADR) (NYSE:IRS) 1.00 Strong Buy
2 Primus Guaranty, Ltd. (NYSE:PRS) 1.00 Strong Buy
3 China Life Insurance Company Ltd. (ADR) (NYSE:LFC) 1.00 Strong Buy
4 National Bank of Greece (ADR) (NYSE:NBG) 1.00 Strong Buy
5 HDFC Bank Limited (ADR) (NYSE:HDB) 1.00 ... 阅读全帖 |
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k****t
发帖数: 2288 | 42 我准备重新编译我openwrt，加入更多的filesystem support进去，支持UFS，FAT32，N
TFS，加上samba。 |
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c*******n
发帖数: 628 | 43 In April of 1965, a single squadron (the 476th TFS) of the 479th
TFW deployed with their F-104Cs to Kung Kuan AB in Taiwan, with
regular rotations to the
forward base at Da Nang Air Base in South Vietnam. Their job was to fly
MiG combat
air patrol (MiGCAP) missions to protect American fighter bombers against
attack
by North Vietnamese fighters. They flew these missions armed with their
single
M61A1 20-mm cannon and four AIM-9 Sidewinder air-to-air missiles. The effect
of F-104 deployment upon NV |
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