n*******u
发帖数: 343 | 1 membrane stain: FM4-64
Ref: Vida TA, Emr SD. J Cell Biol. 1995 Mar;128(5):779-92.
A new vital stain for visualizing vacuolar membrane dynamics and endocytosis
in yeast. |
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m***6
发帖数: 424 | 2 Is there anyone who did lysosome staining?
I need good staining result. But with lysotracker could be done well.
Who could give some suggestions?
Thank you very much. |
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h***e
发帖数: 232 | 3 i'm a new to this.
Just wondering if DAPI can get into live mammalian cells and stain the
nucleus? If yes, why many protocols require cell fixation and
permeabilization?
Will DAPI do anything to membrane protein if it stains live cells?
Many thanks! |
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m******5
发帖数: 1383 | 4 急问做过C12-FDG LacZ staining的同学，在staining的过程中red signal bleed
through 厉害么？ 我目前看到的信号很特意，但red bleed through很严重 |
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b*****n
发帖数: 1841 | 5 组织中有RFP和GFP，如果要染色就只有选蓝光了，而蓝光很弱，另外如果要做co-
staining就没得选择了。
有没有办法把RFP和GFP的荧光彻底bleach掉，但是不影响后续的staining？
谁给个详尽的protocol，有包子。 |
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b******9
发帖数: 425 | 6 做了CD31的IHC staining，想quantify staining的面积，算vessel density。请问该
用什么软件呢？ 最好是免费的。多谢多谢！ |
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h****k
发帖数: 182 | 7 有一个抗体在reduced sds gel 上识别我要的蛋白很好,但是non reducing gel上不识
别,
如果要下一步做tissue stain 是做paraffin section 还是oct 包裹的section? 谢谢
了 |
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q******g
发帖数: 3858 | 8 好像一般用Hoechst
https://en.wikipedia.org/wiki/DAPI
DAPI can be used for fixed cell staining. The concentration of DAPI needed
for live cell staining is generally very high; it is rarely used for live
cells |
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c****e
发帖数: 252 | 9 Does anyone know how to stain the polyethylene thin sections for TEM
observation?
I want to discern the amorphous and crystally part. I used the RuO4 stained
the thin sections for 30 minutes, but I can't see anything under TEM. Any
suggestions?
Thanks |
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e********t
发帖数: 9607 | 10 不想paint，想stain保持木纹，但是stain可以让木头颜色变得比原来的自然色还浅吗？ |
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w*******y
发帖数: 60932 | 11 Anniversary Sale
Save 40%* on paints an stains!
Find your participating
Sherwin-Williams store
We're celebrating years of great colors, great advice and
great customers with a special savings event. Join us for the
biggest sale of the year at you local Sherwin-Williams paint store!
One Day Only ● Saturday, July 17th
40%* OFF paints and stains
30%* OFF painting supplies
10%* OFF wallcovering books
*Retail sales only. Discount taken off of full retail price. Sale pricing or
other offers that |
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w*******y
发帖数: 60932 | 12 First go to your coupon book and look for the $3 Tide Stain release coupon,
then select the Tide Stain Release Duo Pac In-Wash Booster Twin Pack, 34-
count Bag, choose subscribe and save (you may cancel at any time after your
order ships). Total should be $8.04
COUPON BOOK [amazon.com]
PRODUCT LINK [amazon.com]
Items: $12.99
Shipping & Handling: $7.44
Shipping Discount -$7.44
Subscription Discount -$1.95
Your Coupon Savings -$3.00
Total Before Tax: $8.04
Estimated |
|
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w*******y
发帖数: 60932 | 14 Link:
http://www.drugstore.com
has Rembrandt Whitening Toothpaste on sale:
Link:
http://www.drugstore.com
for $6.99 each
and
Link:
http://www.drugstore.com
for $.5.99 each
There are 2 specials:
1. Buy any Rembrandt item and receive a free Deeply White+Peroxide
toothpaste ($6.99 value!)
2. $5.00 off $20.00 JandJ oral care products
and Free shipping with $25 purchase.
Purchase 1 x Rembrandt Intense Stain Whitening Toothpaste with Fluoride Mint
and 3 x Rembrandt Deeply White + Peroxide White... 阅读全帖 |
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w*******y
发帖数: 60932 | 15 Sherwin Williams [Store Locator:
http://www.sherwin-williams.com/store_locator/store.jsp
] is having a "4-day Super Sale" on paints and stains from November 4 - 7,
2011.
40% off paints and stains*
30% off painting supplies *
*Retail sales only. Discount taken off of full retail price. Sale pricing or
other offers that result in greater savings will supersede this offer.
Excludes Multi-Purpose primer, Design Basicspaint, MinwaxWood Finishes
Quarts, ladders, spray equipment & accessories and gift ... 阅读全帖 |
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l*******m
发帖数: 3346 | 16 我对于怎么stain地板毫无概念，做的那天是不能有人在的，之后要４８小时什么的。
但我看到了厨柜的stain, 很难弄的很均匀。我们第一遍找的老墨做的很不好，我们又
找人重做了一遍。这个似乎技术含量还是满大的。 |
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B********t
发帖数: 180 | 17 180->220 sand了，然后stain. |
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p******g
发帖数: 62 | 18 stain看上去与eggshell差别大吗？会不会亮很多呢？ |
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T*********e
发帖数: 9208 | 19 你开始问的是stain,
Satin: This paint style has all the benefits of eggshell. It is more readily
available and washes somewhat better. Satin does not hold up to any vigorou
s scrubbing, however. Satin paints do not show brush marks like the glossier
styles.
Semi-gloss: Although this is the costliest of the paints, it is the most use
ful for rooms which get a great deal of use, like children's bedrooms, playr
ooms, bathrooms, laundry rooms and kitchens. Semi-gloss has a nice
sheen and washes up very wel |
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h**h
发帖数: 238 | 21 地板磨好了，接下去是先地板stain刷清漆，还是先把墙给刷了？多谢？ |
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w*******g
发帖数: 9932 | 22 It is no fun at all to have a big deck. Lots of maintainence. Staining deck
is
a back-breaking labor and you have to do it at least every other year. |
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w*******g
发帖数: 9932 | 24 just soap and powerwash. The deck got quite a bit of mold growing on it a
few
months ago when I bought the soap to remove it. It is prudent to clean the
deck
before spending hours to stain it. Otherwise, you just lock the dirt inside. |
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m***y
发帖数: 14763 | 25
did you use roller? Brush or spray should do the work.
Really your call.
Rent a sander from HD or other stores.
Depends on the previous paint.
Yes, stain/paint does protect the wood from weathers. |
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m**a
发帖数: 1228 | 26 东北地区，这两天白天55度，晚上40度左右
会不会太冷，stain一上去就干了？
刚买了房子，怕放到明年开春，deck都得让雪水泡坏了。 |
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s**********n
发帖数: 291 | 27 您这意思就是大冷天stain对木头不好？今冬俺们家的deck就只能赤膊上阵了？ |
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s**********n
发帖数: 291 | 28 ok i see. i guess i would just stain it as all of the hard work has already been done. we plan to do it again within next couple years anyway. this deck has not been washed for a decade by the previous owner.
Thanks a lot for the advice.
So
power |
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w*******g
发帖数: 9932 | 29 都是普通的漆. stain一般是半透明的
Wood |
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w*******g
发帖数: 9932 | 30 solid stain = wood paint. 在我看来就是刷漆. 刷新漆最要先把老漆搞掉. |
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c****o
发帖数: 32446 | 31 一般钉子眼都在墙边了，stain不好看也不要紧啦 |
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m***y
发帖数: 14763 | 32 While we are on it, here's what I really do. Wood filler are sold in
different shades, usually we can't find an exact match. So, buy one darker
and one lighter. After the stain, take some darker and some lighter, and mix
them up. Depending on your skill level, your mix could get very close to
the desired shade. Of course, always err on the darker side. |
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l*****o
发帖数: 26631 | 33 有一些黑乎乎的stain， 不知道是什么东东...
有没有人有经验呀？ |
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H******7
发帖数: 34403 | 34 很久以前买了桶Concrete Stain，今天准备用。打开一看，居然是白色的。是不是当时
我忘记了配
色？现在有什么办法配色吗？DIY或找HD可以帮助配吗？ |
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m**********g
发帖数: 2661 | 35 买的房子里有original的built-in的一些家具，细节很漂亮，保持的也挺好，原来的木
纹也不错，但是颜色太深了。如果上白油漆那些木纹就可惜了，线脚也不会有现在的挺
了。有人告诉我说有一种东西喷上去可以让stain去掉露出木头的本色，然后再刷清漆
。但是他也不清楚这东西叫什么，这里有人知道吗？谢谢。 |
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e********t
发帖数: 9607 | 36 我的经验。只有机械的方法：就是磨掉一层。灰很大。
市面上的stripper，对stain基本无效。 |
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h**********r
发帖数: 2284 | 37 That's why many people stain a deck, instead of paint it.
To sand the peeling paint off seems to be the only choice unfortunately. |
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s****a
发帖数: 501 | 38 deck基本用sander打磨干净了，有些木头已经有了裂缝，可以power wash之后直接上
semi-tranparent wood stains吗？还是需要先用sealed之类的东西？多谢！ |
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s****a
发帖数: 501 | 39 wood filler是必须用还是可用可不用？其实这些裂纹也不大，并不影响美观。如果
stain能够渗入到裂缝中起到保护作用的话，就不想用了。 |
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a****e
发帖数: 706 | 40 inspector就在报告上写着有water stain，但又没说什么原因，你们一般这种情况都是
怎么处理的。谢谢。。。 |
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b2
发帖数: 427 | 41 现在这个deck floor 是composite，状态还不错，好像不用收拾，邻居说除非要换颜色
，否则不用清洗。
我有个问题，首先如何判断现有颜色是板子自己带来的还是后来刷上去的？stained 是
指把颜色渗透到板子里么？
这个composite deck平时需要清洗么？可以用pressure washer 么？
因为deck floor 上面的的栏杆是普通的木头，被蜜蜂搞了好多的洞，怎么都要清洗刷
漆了，想趁着租pressure washer一起把floor也清洗了。
不知道说的明白否？
谢谢大侠的帮助 |
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p*****y
发帖数: 1579 | 42 请大侠们推荐一下用什么刷下deck比较好。
我看有sealant, wood stain, wood finish, oil based, acrylic各种各样，完全晕了。
主要目的就是保养一下，防止裂缝阿什么的。deck是深红色的木头。
谢谢！ |
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f****i
发帖数: 20252 | 45 stain好比书上泼咖啡,纸黄了,字还在
paint好比泼涂改液 |
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f****i
发帖数: 20252 | 46 木头好的话，stain好看
烂木头的话，paint好看 |
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c*h
发帖数: 33018 | 47 Thanks for the questions and answers。受教育了。我家的DECK是stain的。 |
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H******7
发帖数: 34403 | 49 是不是因为stain的不怕wear? 小的damage看不出来？ |
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