r**********e
发帖数: 587 | 1 很感谢。虽然笨但很好
我就是想问下，如果从最简单最快的pcr sanger sequencing看到差别，那么有多少把
握／信心接下来的精确定量会有显著差别呢？ |
|
r******f
发帖数: 987 | 2 一直做有关核酸的研究，但是现在next generation sequencing 的工作很火，想学些
这方面的技术，可是现在的实验室一点也不做这个。想换个会用到这个技术的实验室，
怎么找呢？google搜索，只能搜出很多中心实验室，帮别人测序的。
大牛们帮忙指点下迷津吧，一般做什么研究方向的会用到这个技术呢？比如说我想在某
个大学里面找个用这个技术的实验室，怎么入手找呢？我搜了一晚上也不得要领。 |
|
r******f
发帖数: 987 | 3 一直做有关核酸的研究，但是现在next generation sequencing 的工作很火，想学些
这方面的技术，可是现在的实验室一点也不做这个。想换个会用到这个技术的实验室，
怎么找呢？google搜索，只能搜出很多中心实验室，帮别人测序的。
大牛们帮忙指点下迷津吧，一般做什么研究方向的会用到这个技术呢？比如说我想在某
个大学里面找个用这个技术的实验室，怎么入手找呢？我搜了一晚上也不得要领。 |
|
r******f
发帖数: 987 | 4 谢谢楼上。我就是想学学怎么分析sequence出来的数据，好找公司的工作。我自己擅长
做样品提纯制备，但是貌似没什么公司招这个的。 |
|
H****N
发帖数: 997 | 5 【 以下文字转载自 Faculty 讨论区 】
发信人: HENBEN (benben), 信区: Faculty
标 题: 请大家推荐个用过的靠谱的nex-gen sequencing 公司。
发信站: BBS 未名空间站 (Fri Jun 30 09:48:14 2017, 美东)
学校的core太慢了。 |
|
G***G
发帖数: 16778 | 6 I got some peptide sequence in the result from thermo discoverer 2.2.
But I don't know the meaning of the letter and dash symbol in the bracket.
For example, what is the difference between [-E] and [E-]?
Can anyone shed some lights on this?
[-].ENPVLDIVSNPEQSAVEQGDSNSSFNEHLK.[-E]
[-].ENPVLDIVSNPEQSAVEQGDSNSSFNEHLKEK.[-]
[-].ENPVLDIVSNPEQSAVEQGDSNSSFNEHLKEK.[-]
[-].ENPVLDIVSNPEQSAVEQGDSNSSFNEHLK.[-E]
[R-].ASQEPFSPAEDVMETDLLEGLAANQDRPSK.[-]
[-].GSGEKPASAPVDDTESLHSQGEEEFEMPQPPHGHVLHR.[-]
[R].FSPVLG... 阅读全帖 |
|
D**A
发帖数: 311 | 7 没想到投稿就要提供sequencing raw data, 有点措手不及，编辑推荐GEO。
据说GEO要5个工作日才可以approve，我们在想可不可以先传到dropbox. |
|
t*****m
发帖数: 73 | 8 需要有确切amino acid sequence的Monoclonal antibody，作为mass spec的标准物质
。无所谓biological specificity。
哪里能买到？ |
|
b*********2
发帖数: 190 | 9 Not sure if there's such mAb that's commercially available.
Waters has mAb standard for mass check. The molecular fomular is provided so
I bet they know the sequence. You can ask them if they are willing to
release the info... |
|
c****o
发帖数: 49 | 10 Keep integrating, gain grows back. Sequence timing is important
理论上DSSS信号起到扩频效果，所以即使信号在thermal noise floor以下，乘以PN
code之后还是可以还原成原来的信号的对吧但是实际上是要用ADC出来的dig........ |
|
x*****d
发帖数: 427 | 11 If the metric space is the set Q of rational numbers,
you see the Cauchy sequence 3.1, 3.14, 3.141, ... which
does not converge in the space. |
|
w**a
发帖数: 1024 | 12 assume the space is complete.
Must any Cauchy sequence converge to some limit? seems that's the definition
for completeness? |
|
X*****r
发帖数: 2521 | 13 那些random sequence会weakly converge to Wiener Process？
我只知道好像i.i.d.的一组RV，然后某个函数是wiener，就是dansker theorem那个
还有别的情况吗？
多谢！ |
|
l******o
发帖数: 1550 | 14 for a sequence , x_(m,n)
is it true that
lim_m ( lim_n ) (x) = lim_n ( lim_m ) (x) (if exist, or even bounded)
iff lim_m (x),lim_n (x) exist for any n and m? |
|
K******S
发帖数: 10109 | 15 【 以下文字转载自 Biology 讨论区 】
发信人: KingofMS (offer来了，H-1B没了。我苦), 信区: Biology
标 题: Please recommend a place to do N-terminal sequencing of protein/peptides
发信站: BBS 未名空间站 (Fri Nov 10 09:38:37 2006)
Cost is the least factor we concern as long as the service is reliable and
the less sample amount they need, the better for us.
Thank you very much. |
|
D**u
发帖数: 204 | 16 【 以下文字转载自 Mathematics 讨论区 】
发信人: DuGu (火工头陀), 信区: Mathematics
标 题: Ordering a sequence (2)
发信站: BBS 未名空间站 (Wed Dec 16 12:17:39 2009, 美东)
Let x1,...xn,y1,...,yn be 2n distinct real numbers, and xi+xj is not equal
to yk+yl for any i,j,k,l.
Prove that you can properly reordering x1,...,xn to z1,...zn, such that
(zi + zj - yi - yj)*(zi - zj)*(yi - yj) > 0
for all i and j. |
|
c**********e
发帖数: 2007 | 17 Suppose I have a continuous variable X and a frequency variable Y. I fit a
model by SAS GENMOD. The model says that Y has a negative binomial
distribution with mean a+bX (or exp(a+bX)).
Specifically, I have a sequence X1, X2, ..., Xn and Y1, Y2, ..., Yn. How to
test Yi's follow negative binomial distributions with mean a+bXi (or exp(a+
bXi))?
Anybody has an idea? Thanks a ton. |
|
a********e
发帖数: 78 | 18 这个random sequence由3000 个点组成，符合normal distribution， mean 是 c,
standard deviation 是d.
但这3000 个点 都落在[a,b]的范围里。
请问应该怎么做，
假如画图的话，y 轴应该是这3000个点， 那x轴的点如何产生？ |
|
a******e
发帖数: 119 | 19 现在二年级，统计专业。要选导师选方向了。自己是生物背景的。系里有个老师是做
Next generation sequencing 数据分析这个方向的,我比较感兴趣。请问班上的大牛们
，这个方向在工业界和学术界怎么样？ |
|
p********y
发帖数: 15 | 20 现在PhD第二年了，跟biochemistry的老板做了一年sequencing analysis，我们做的是
植物的DNA,现在做的东西基本没什么fancy的统计应用，大多数时间都是在处理数据上
了，统计方面就只用得到一些基本的test就完了，读了些paper也都是偏生物的，本人
才疏学浅，想问问有没有对这方面有研究的，给我指指路，我怕这样下去统计的东西都
荒废了啥都用不上，最后论文写不出个什么来就悲惨了 |
|
h****6
发帖数: 229 | 21 Does anyone know a conserved chloroplast sequence across different species?
Appreciate it very much if anyone can offer some information!!! |
|
d*****r
发帖数: 39446 | 22 【此篇文章是由自动发信系统所张贴】
sequence 已经成为本俱乐部的正式成员, 特此通知. |
|
a****o
发帖数: 1786 | 23 【 以下文字转载自 Biology 讨论区 】
发信人: dfbb (东方不败), 信区: Biology
标 题: next generation sequencing technology
发信站: BBS 未名空间站 (Wed Jun 23 21:30:45 2010, 美东)
1990年，人类基因组计划开始；2000年草图完成；2001年发表。这第一个基因组的耗材
（consumables）花费估计有$300,000,000。而最近发表的几个被测序的基因组费用是：
$250,000 （Illumina，2008）$48,000（Helicos，2009）和$4,400（Complete
Genomics，2010）。基因组测序越来越便宜了，以后还会降到$1,000甚至更低（中国人
的计划是
￥5,000）。
这些简单的数字背后是测序技术和仪器研发的激烈竞争。先简单列一下有哪些公司吧，
Illumina
Genome Analyzer，Life Technologies的SOLID，Roche 454，Pacific Biosciences
（PacBio），Complete |
|
C*I
发帖数: 4736 | 24 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 25 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 26 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
|
发帖数: 1 | 27 http://theprepared.com/blog/no-the-2019-ncov-genome-doesnt-actually-seem-engineered-from-hiv/
2019-nCoV genome doesn’t really seem engineered from HIV
A group of bioinformaticians at two prestigious universities in Delhi, India
, published a preprint scientific manuscript on the bioRxiv preprint server
Friday has led many to speculate wildly that 2019-nCoV may have been
deliberately engineered using HIV protein sequences.
The paper, entitled “Uncanny similarity of unique inserts in the 2019-nCoV... 阅读全帖 |
|
g*********d
发帖数: 233 | 28 科学家呼吁关注全球基因组数据库污染
样品处理有可能是导致DNA数据库广泛污染的最主要原因
2月16日发表在《公共科学图书馆·综合》（PLoS ONE）期刊上的一份研究报告称康涅
狄格大学的遗
传学家Mark Longo及同事发现由顶级公共测序机构提供的测序结果构建的基因组数据库
中的大约
1/5的细菌、植物和非灵长类动物基因组数据受到了人类DNA的污染，样品处理有可能是
导致DNA数据
库广泛污染的最主要原因。这一研究报告引起了生物研究人员及各大权威媒体的高度关
注，《科学
家》（The Scientist）杂志以及《自然》（Nature）杂志均在其官方网络上第一时间
对这一事件
进行了报道。
Mark Longo等在报告中呼吁科学家们需更加努力以确保测序获得的基因组不受到污染，
并应对来自
公共基因组数据库的基因组进行潜在污染检测。
“基因组污染是一个大问题，但却不是一个新问题，”加州大学进化生物学家、美国能
源部联合基因组
研究所系统发育基因组学计划负责人Jonathan Eisen说：“这篇论文或可帮助提醒人们
注意这一问
题。”
污染有可能在测序的任何一个阶段导入到基因组序列中... 阅读全帖 |
|
d****n
发帖数: 233 | 29 Paste a solution with O(mn) complexity(coded in c#):
static int FindMaxWater(int[,] matrix, int m, int n)
{
// a 2d array with same size as matrix to hold maxim waters
int[,] potentialWater = new int[m, n];
List sequence = new List();
for (int i = 0; i < m; ++i)
{
// Find the ascending sequence from left to right
sequence.Clear();
sequence.Add(0);
for (int j = 1; j < n; ++j)
{
if (matrix[i, ... 阅读全帖 |
|
d****n
发帖数: 233 | 30 Paste a solution with O(mn) complexity(coded in c#):
static int FindMaxWater(int[,] matrix, int m, int n)
{
// a 2d array with same size as matrix to hold maxim waters
int[,] potentialWater = new int[m, n];
List sequence = new List();
for (int i = 0; i < m; ++i)
{
// Find the ascending sequence from left to right
sequence.Clear();
sequence.Add(0);
for (int j = 1; j < n; ++j)
{
if (matrix[i, ... 阅读全帖 |
|
J*****3
发帖数: 4298 | 31 笑死我了，还来玩黑白颠倒的吗？
我给你全文吧，这么撒谎的话，是耶稣教的吗？撒谎神不喜悦
这篇文章是基督教手册用来歪曲人类进化不成立的，是JAMES给我了文章的一个LINK，但是文章看不全，我根据作者名字找到了全文，也读了，发现，文章根本没有说进化不成立，他们断章取义，黑白颠倒地撒谎。我BS这种恶心的行为。
“5、大概在97年，一个著名的非基督徒考古学家，在非洲发现的所谓人类化石，把一
直以来公认的人类进化顺序，猿人——直立人——能人——智人，给彻底推翻了。目前
人类进化链条重新归零，变为空白。”
原文
http://news.nationalgeographic.com/news/2010/05/100506-science-
翻译：
“Even if the new result doesn't quite settle the debate about whether
Neandertals mixed with modern humans, it does underscore how different they
were from our own lineage. And t... 阅读全帖 |
|
f**d
发帖数: 768 | 32 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
|
R****a
发帖数: 6858 | 33 大棋局与9/11
来源: ognc 于 2013-02-22 16:08:44[档案] [博客] [旧帖] [转至博客] [给我悄悄
话] 本文已被阅读：63次
字体：调大/调小/重置 | 加入书签| 打印| 所有跟帖 | 加跟贴| 查看当前最热讨论主题
The Moral Decoding of 9-11
By Prof. John McMurtry
(Journal of 9/11 Studies) - I was sceptical of the 9-11 event from the first
time I saw it on television. It was on every major network within minutes.
All the guilty parties were declared before any evidence was shown. The
first questions of any criminal investigation were erased. Who had the most
compelling motives for... 阅读全帖 |
|
F*V
发帖数: 3978 | 34 大棋局与9/11
来源: ognc 于 2013-02-22 16:08:44[档案] [博客] [旧帖] [转至博客] [给我悄悄
话] 本文已被阅读：63次
字体：调大/调小/重置 | 加入书签| 打印| 所有跟帖 | 加跟贴| 查看当前最热讨论主题
The Moral Decoding of 9-11
By Prof. John McMurtry
(Journal of 9/11 Studies) - I was sceptical of the 9-11 event from the first
time I saw it on television. It was on every major network within minutes.
All the guilty parties were declared before any evidence was shown. The
first questions of any criminal investigation were erased. Who had the most
compelling motives for... 阅读全帖 |
|
g****g
发帖数: 1828 | 35 SGI Logo
vector
Category: containers Component type: type
Description
A vector is a Sequence that supports random access to elements, constant
time insertion and removal of elements at the end, and linear time insertion
and removal of elements at the beginning or in the middle. The number of
elements in a vector may vary dynamically; memory management is automatic.
Vector is the simplest of the STL container classes, and in many cases the
most efficient.
Example
vector V;
... 阅读全帖 |
|
x****6
发帖数: 4339 | 36
The occurrence of concentrated pneumonia cases in Wuhan city, Hubei province
of China was first reported on December 30, 2019 by the Wuhan Municipal
Health Commission (WHO, 2020). The pneumonia cases were found to be linked
to a large seafood and animal market in Wuhan, and measures for sanitation
and disinfection were taken swiftly by the local government agency. The
Centers for Disease Control and Prevention (CDC) and Chinese health
authorities later determined and announced that a novel coro... 阅读全帖 |
|
C*I
发帖数: 4736 | 37 相比较而言，石正丽的《Bat origin of human coronaviruses/蝙蝠是人类冠状病毒的
来源》是扎扎实实的抓蝙蝠，解剖蝙蝠，分离病毒，对比DNA,RNA后得出得结论。
请lobbock读一读，说一说，走两步！
http://virologyj.biomedcentral.com/articles/10.1186/s12985-015-0422-1
Review
Open Access
Published: 22 December 2015
Bat origin of human coronaviruses
Ben Hu, Xingyi Ge, Lin-Fa Wang & Zhengli Shi
Virology Journal volume 12, Article number: 221 (2015) Cite this article
Abstract
Bats have been recognized as the natural reservoirs of a large variety of
viruses. Special attention has ... 阅读全帖 |
|
m******e
发帖数: 353 | 38 #include
#include
void countOnesAndZeros(int &numZeros, int &numOnes, const std::vector &
sequence) {
numOnes = 0;
numZeros = 0;
for(size_t i = 0; i
sequence[i] == 1 ? ++ numOnes : ++ numZeros;
}
}
int trimLeft(const std::vector &sequence, int start, int numToTrim, int
untilSeenSymbol) {
int trimCnt = 0;
while(start + trimCnt >= 0 && start + trimCnt < (int) sequence.size() &&
numToTrim > 0) {
if(sequenc... 阅读全帖 |
|
m***T
发帖数: 11058 | 39 NGS市场又要热闹了。
http://www.bio-itworld.com/news/09/17/12/Sequencing-giants-BGI-
Sequencing Giants BGI and Complete Genomics Announce Merger Agreement
By Bio-IT World Staff
September 17, 2012 | In a stunning move for the genomics industry, two
leading DNA sequencing service providers – China’s BGI and Complete
Genomics – have entered into a definitive merger agreement.
In a prospective deal worth some $117 million, BGI Shenzhen has tendered an
offer of $3.15/share for all of Complete’s outstanding ... 阅读全帖 |
|
发帖数: 1 | 40
Download pdf published by SfN (Chapter from 'The History of Neuroscience in
Autobiography, Volume 8' edited by Larry R. Squire)
Yuh-Nung Jan's CV
Lily Jan's CV
Yuh-Nung Jan and Lily Jan
Birth
Family History and Growing Up
National Taiwan University
The Hiking Trip to Shitou in the Spring of 1967
Graduate School Application
Graduate Study at Caltech (1968锟974)
Seymour Benzer Lab (1974锟977)
Steve Kuffler锟絪 Lab at H... 阅读全帖 |
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v*****k
发帖数: 7798 | 41 The new frontier of genetic analysis is developing so fast that industry
leader Illumina plans to come out with an iPad app.
Some of Illumina’s ILMN software used in gene sequencing already
runs in the Internet cloud.
Illumina showed off its new “MiGenome” iPad app at the third annual
Consumer Genetics Show in Boston on June 8, pointing out that it will put
genomic information at a person’s fingertips.
Illumina will soon start shipping a lower-price gene sequencing system in
the... 阅读全帖 |
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l**********1
发帖数: 5204 | 42 linker-based PCR
plus
iMapper soft:
//www.ncbi.nlm.nih.gov/pubmed/18974167
//geocachingsoftware.com/imapper.html
more details
please to to E-Book
Chapter 2:
its link:
FTP://ftp.sanger.ac.uk/pub4/theses/kong/chapter2.pdf
citation:
>Based on these expectations, a web-based server called iMapper (Insertional
Mutagenesis Mapping and Analysis Tool) was developed for the efficient
analysis of insertion site sequence reads against vertebrate and
invertebrate Ensembl genomes. Taking linker-based PCR se... 阅读全帖 |
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s****l
发帖数: 10462 | 43 NIH最近又房顶了八个小组，大家看看哪一个最有可能成功？
The new grants are awarded (pending available funds) to:
University of California Santa Cruz, $2.29 million over three years
Principal Investigator: Mark Akeson, Ph.D.
Investigators plan to sequence single DNA molecules by using a nanopore
device comprised of a sensor that touches, examines and identifies each
nucleotide, or DNA building block, in a DNA strand as an enzyme motor moves
it through the pore. The scientists will focus on DNA “resequencing” —
examining th... 阅读全帖 |
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s****l
发帖数: 10462 | 44 NIH最近又房顶了八个小组，大家看看哪一个最有可能成功？
The new grants are awarded (pending available funds) to:
University of California Santa Cruz, $2.29 million over three years
Principal Investigator: Mark Akeson, Ph.D.
Investigators plan to sequence single DNA molecules by using a nanopore
device comprised of a sensor that touches, examines and identifies each
nucleotide, or DNA building block, in a DNA strand as an enzyme motor moves
it through the pore. The scientists will focus on DNA “resequencing” —
examining th... 阅读全帖 |
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r*****m
发帖数: 3619 | 45 Results: 46
Select item 25597018
1.
Exome-wide Sequencing Shows Low Mutation Rates and Identifies Novel Mutated
Genes in Seminomas.
Cutcutache I, Suzuki Y, Tan IB, Ramgopal S, Zhang S, Ramnarayanan K, Gan A,
Lee HH, Tay ST, Ooi A, Ong CK, Bolthouse JT, Lane BR, Anema JG, Kahnoski RJ,
Tan P, Teh BT, Rozen SG.
Eur Urol. 2015 Jan 14. pii: S0302-2838(14)01396-7. doi: 10.1016/j.eururo.
2014.12.040. [Epub ahead of print]
PMID: 25597018 [PubMed - as supplied by publisher] Free Article
Related citations... 阅读全帖 |
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S*****P
发帖数: 323 | 46 http://news.sciencemag.org/scienceinsider/2011/06/sequence-yiel
DNA Sequence Yields Clues to Germany's 'Super Toxic' E. coli outbreak
by Martin Enserink on 2 June 2011, 5:50 PM |
Just from the high number of deaths and severe cases, scientists and public
health experts battling Germany's massive E. coli outbreak knew they were up
against something unusual. Now, early results from sequencing projects of
the enterohemorrhagic Escherichia coli (EHEC) strain appear to confirm that
a never-before-see... 阅读全帖 |
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l******u
发帖数: 936 | 47 http://investor.illumina.com/phoenix.zhtml?c=121127&p=irol-news
Illumina’s MiSeqDxTM Receives FDA Premarket Clearance with Two Cystic
Fibrosis Assays and Universal Kit for Open Use
Premarket Clearance is an Industry First for a Next-Generation Sequencing
System
SAN DIEGO--(BUSINESS WIRE)--Nov. 19, 2013-- Illumina, Inc. (NASDAQ:ILMN)
today announced that it received premarket clearance from the U.S. Food and
Drug Administration (FDA) for the MiSeqDx system, the first high-throughput
DNA sequencin... 阅读全帖 |
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P*****e
发帖数: 25 | 48 今天有空去查了一下CRISPR专利的历史，UCB的专利已经有claim通过了审查（allow）
，只是还没有issue（时间问题）。我在后面列出来了其中一个通过了的claim，同时把
张锋issue了的专利里面的一个claim也列了出来。大家看能避开不？张锋后来的Cpf1看
着能避开UCB的Cas9。
有意思的是发现最早的一篇CRISPR相关专利申请其实是Luciano A. Marraffini（张锋
合作者）在Northwestern University时申请的，priority date Sep 23, 2008，早了
好几年。看看claim，只有interference，还没进化到切割，可惜一个claim也没批，居
然华丽丽的被一堆112给毙了，也就是审查员觉得那专利写的不清不楚，业内普通人士
看着不可能做出claim的结果，连101，102，103都没用上就挂了。西北也是早早的
abandon了，当年连PCT都没申请，可见并不重视。张锋专利里也没有Marraffini，虽然
science文章有他，我猜可能也是他只做真核interference方面的工作吧，张跟他合作
并进... 阅读全帖 |
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o*****D
发帖数: 1563 | 49 U.S. Clears DNA Firm’s Acquisition by Chinese
The federal government has given national security clearance to the
controversial purchase of an American DNA sequencing company by a Chinese
firm.
The Chinese firm, BGI-Shenzhen, said in a statement this weekend that its
acquisition of Complete Genomics, based in Mountain View, Calif., had been
cleared by the federal Committee on Foreign Investment in the United States,
which reviews the national security implications of foreign takeovers of
America... 阅读全帖 |
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j*p
发帖数: 411 | 50 本人在wet lab里面做纯数据分析，for NGS data analysis, 简单介绍一些自己接触过
，并且觉得挺有用的工具，说的有点杂，权作抛砖引玉，还请不吝赐教。
Next-Gen sequencing(NGS)和现在正在发展的3rd-gen sequencing将会在生物学研究中
被越来越广泛应用。不管你信不信，反正我信了。一是基于实验成本的降低（$1k
whole-genome sequencing is coming），越来越多的实验室可以操作；二是可以提供
相对low throughput experiment多的多的数据和信息，可以看到很多从前看不到的东
西；三是sequencer本身对测序的准确性正在逐渐提高，所以实验固有错误率降低；四
是各种算法的成熟应用，这使得很多由于实验产生的误差在出数据后通过对数据的分析
得以过滤。按照library preparation来分，NGS主要有DNA-seq和RNA-seq
DNA-seq is usually used as ChIP-seq to study transcription factor(TF)-DNA
bi... 阅读全帖 |
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