e****e 发帖数: 1042 | 1 I already got what I need to know. My question is very simple. That is, is
there any method can measure only protein but no peptide without further
seperation or purification? I thought the answer should be "No" before I
came here. But I am not so sure since I never think about it before. When I
worked in purification labs, we always used OD280 to determine protein
concentration since the protein is quite pure. When I worked in molecular
biology lab, we always used Bradford to determine the pro... 阅读全帖 |
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A******4 发帖数: 610 | 2 老板让我搞gas adsorption,现在我的方向是空分air seperation by adsorption. 但
这个方向比较成熟了,比较好的是hydrogen storage, 我想知道gas adsorption or
adsorption 这个领域怎么样以及我的air seperation还可以转到gas adsortption的那
些方向 |
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t***t 发帖数: 7 | 3 关于第二条,我觉得他的意思是说,人和工作有好的match,也有不好的match,on the
job matching模型跟这个有些关系,基本意思是说工人每一期都可以draw一个新的off
er,然后比较接受这个新offer的价值和继续原来的bad match的工作的价值,因为firm
specific human capital的积累,这个bad match的工作的价值越来越高,所以这个模型
的一个推论就是单个工人seperation hazard随时间递减,这个推论是没办法检验的;另
一个可以检验的推论是工人平均的seperation hazard也随时间递减
不过wage gain只是这个模型的一个假设(假设有好的和坏的match),模型是为了解释se
peration hazard的时间趋势以及工资与tenure的关系的
导 |
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c**b 发帖数: 474 | 4 you can run the two-part model seperately by STATA, or any other software.
Actually, it contains two parts, i.e. two independent regression. Usually,
first part is a probit or logit;second part depends on your dependent
variable, which could be any regression, such as OLS, Negative Binomial
conditional on something,etc. Usually, when you run two-part model, you need
to report the results of each part. Therefore, you need to run it
seperately. |
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c*s 发帖数: 2145 | 5 let me try to explain
it is well known that teh polymer blend or block polymerwill phase seperation
when dried. depending on the ratio, they may have different morphology.
but, the phase seperation should occur at teh length scale of tens or hundred
nanometers, for example, for 100k molecular weight, the size should be ~100nm.
In your case, it is much much larger than that.
therefore, the reason is probably that you have not mixed the two materials
thoroughly. and the materials simply formed dif |
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c*s 发帖数: 2145 | 6 to seperate thiol capped nanoparticles, I think you can use
several methods.
1. add some solvents to make phase seperation, commonly used
solvents such as methanol, acetone or iso-propanol, this
method usually works, if th esolution is rotary evaporate
first, th eeffect is better
2. you can dialysis th esolution first to remove some
by-product, such as salts(supposed there exists some salts
in th esolution) after that, the particle became instable
and precipitate occurs, then you can use centrig |
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p*****r 发帖数: 334 | 7 【 以下文字转载自 Automobile 讨论区 】
发信人: patcher (YY), 信区: Automobile
标 题: 2001 SAAB 93 换low control arm, 巧修座位螺叩
发信站: BBS 未名空间站 (Sat Nov 12 19:15:57 2011, 美东)
2001 saab 93 SE,120000 mile
低速是右轮处有时会咔哒响一下,低档加速会有吱吱的声音,踩刹车也会咔哒响一下。
刹车片还很好。想来是ball joint 的问题,特来求证一下。
把轮胎卸了,证实是ball joint 的问题。打算自己换,好像要和low contral arm 一
起换,不能单独换 ball joint.
1, 要不要两边都换了,有的说另一边也会很快就坏了。
2,ebay 上有的low contral arm 是93 和900可以通用,但是有的只能用于900. 这个
不知有什么讲究。
3,换完后要做alignment 不。
有换过的,给个指导意见,谢谢了。我换完了会发个技术贴
现已换了,一切问题都没了。在ebay买的配件70多刀,到local店要100... 阅读全帖 |
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w*******y 发帖数: 60932 | 8 Walgreens has 2 liters of 7up, Dr. Pepper, Sunkist, Canada Dry, A&W (and
their diet counterparts) for .99 and you get .99 in register rewards back!
You can do it over and over but don't roll your register rewards and ring
each up seperately. Sale goes through Sunday the 11/28.
I bought like 30 today and every different cashier let me do seperate orders
over and over with no problems.
I did a search but didn't find this. Would love any reps.
|
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h*******n 发帖数: 2052 | 9 【 以下文字转载自 Boston 讨论区 】
发信人: honestman (snail), 信区: Boston
标 题: 麻省报税疑问, 请大家务必帮忙看看, 谢谢!
发信站: BBS 未名空间站 (Thu Apr 15 20:19:22 2010, 美东)
我LD在外州读书, 所以报税的时候按照married but file seperately报, 麻州规定
租房子可以抵3000刀, 但是married but file seperately一个人只能抵1500刀, 但
是我LD并不报麻省州税, 所以也不claim另外这1500刀, 请问我如何才能抵3000刀房
租呢? |
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m*****i 发帖数: 1205 | 10 Not so expensive. I got it $47.99. It has very nice packaging,
long box, with 3 seperate photos of Takuya and Takako (not signed
though:). I had no problem playing it on my computer (and I don't have
a VCD player seperately) |
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j***a 发帖数: 832 | 11 要 看 你 所 在 的 州 法 律。 大 多 数 的 地 方 比 如 TX, CA, INHERITANCE
PROPERTY is seperate property. 以 后 他 得 到 房 子 后 要 不 要 加 你 媳 妇
的 名 字 (那 大 多 数 情 况 就 是 共 同 财 产 了 吧 ) 就 是 他 自 己 的 事
情 了 。
那 个 富 人 给 孙 子 钱 是 为 了 免 税 把 , Generation skip. |
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s**a 发帖数: 1906 | 12 cleaner?
You can wash your clothes and body seperately? |
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l****g 发帖数: 15 | 13 here is what I send to my friends
Dear my friends,
There is a donation compitition in facebook, the first and second winner (
organization) will get $50,000 and $25,000 rewards seperately, the rule is
for every organization, everyone can only donate one time on that day,
please pay attention that the winner is caculated by the number of people
donated, not the money accumulated. So $10/day is a good strategy and
affordable way to win the compitition.
Currently the first two positions in lead are |
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m*******g 发帖数: 186 | 14 fnm+fre > citi + wells fargo.
us government owns 25%+ in citi including preferred.
seperately, us owns 75% of gmac, the nineth largest bank holding company
with asset over 200bn. |
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o****y 发帖数: 26355 | 15 Then China and USSR would be united and would not go seperate ways.
They would be all afraid of the threats from America. |
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s****i 发帖数: 2993 | 16
Don't know china, here in the us fire hydrants use seperate pipe line. |
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u****n 发帖数: 7521 | 17 South Korean are composed of: 朝鲜 Choson 31%, 新罗 Shilla 29% (japanese
native Ainu), 百济 18% (Ainu), 高句丽 Koguryo 9% (Han and 女真 mixed );
Han Chinese 9%; 闽南 3% (southern Chinese)
North Korean : 高句丽 45%; Han Chinese 33%; 朝鲜 12%; 闽南 7%;Ainu 3%
N.Korean very different from South in composition,because Koguryo conquored
N.Korea in the history.Later Koguryo was conquored by Chinese Tang denasty
and became China,thereafter 新罗 , 百济 , 高丽 moved to the north.Korean ppl
is highly mixed, seperate by N &... 阅读全帖 |
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w*********g 发帖数: 30882 | 18 平井宪夫揭示日本核电业惊天内幕——素人造核电,豆腐渣工程
作者:蜀中行 更新时间:2011-3-16
设施配管1级技士平井宪夫(1997年1月因癌症逝世)生前的最后吶喊。
/来自中华网社区 club.china.com/
/来自中华网社区 club.china.com/
关于平井宪夫:
1997年1月逝世。设施配管1级技士,核电事故调查国民会议顾问,核电劳工救济中
心代表,北陆电力志贺核电厂停止诉讼原告特别辅佐人,东北电力女川核电停止诉讼原
告特别辅佐人,福岛二号核电厂三号机运转停止诉讼原告证人。「核电劳工救济中心代
表」在他仙逝后,因后继无人关闭
"我不是反核运动家。这20年来,我一直在核电厂工作。社会上有听不完的拥核反
核理论,但我只想在这里告诉大家:「所谓的核电厂是这样一回事。」大部份的人都不
知道核电内部的实际情形。希望大家有耐心把这篇文章读完。所谓核电,跟各位所想的
或许有点出入。在那里,每天都有遭受放射线污染的工人,以及严重的岐视产生。
我的专长,是负责大型化学工场的内部配管施工与维修。快30岁时,日本掀起了一
阵核电建设的... 阅读全帖 |
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o****y 发帖数: 26355 | 19 The poor need the street peddlers ah.
The rich and the poor live seperately is the best solution la.
The rich can not force their rules on the poor.
the poor can not force their rules on the rich.
Everyone is happy. |
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e*u 发帖数: 10016 | 21 seperated就是离啊,又没说离婚divorce,娃你要长进点啊,哎~~~~~~~~~~~~ |
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H****g 发帖数: 14447 | 22 谢谢指正。不过你看这段话,也的确是用seperation来解释的。
Marx's theory of alienation (Entfremdung in German, which literally means "
estrangement"), as expressed in the writings of the young Karl Marx (in
particular the Manuscripts of 1844), refers to the separation of things that
naturally belong together, , or to put antagonism between things that are
properly in harmony.
http://en.wikipedia.org/wiki/Marx's_theory_of_alienation |
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o****y 发帖数: 26355 | 23 They just cannot tell if so many colors mixing together.
If the colors are seperated, some of them can tell the colors apart. |
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y*****y 发帖数: 1208 | 24 you got a stinky mouth, dude.
my point is banks need to publish and itemize every charge. not after the
fact without upfront explanation.
for this wire transaction, my father was told he can't do $50K. $30K max so
he has to send another wire for $20K. for 2 seperate transactions hence two
sets of fees incurred.
We paid 80RMB wired fee and 31RMB processing fee upfront for the $30K
transfer to 建行. 建行 said they will not charge more fees. first, upon our
inquiry, they flatout denied they charge $1... 阅读全帖 |
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y*****y 发帖数: 1208 | 25 To other people replied here, thank you for the input. I am not going to
write back one by one. Here is the summary.
Unlike some said, I didn't expect free lunch. For this wire transaction, my
father was told by CCB he can't do $50K at once. $30K is the max so he has
to send another wire for $20K. For 2 seperate transactions hence two sets of
fees incurred. We are not happy about this policy.
So for the $30K transfer through 建行, we paid 80RMB wired fee and 31RMB
processing fee upfront..... 建行 s... 阅读全帖 |
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f*******e 发帖数: 289 | 26 当地时间4月18日上午,由和田长途(Uzun Seper)维修店制造的小型直升飞机在和田
市开发区进行试飞。后来因前来围观者较多而公安部门阻止继续飞行。最终,这架由和
田维吾尔人制造的直升飞机被载到大卡车带离现场。 |
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c***c 发帖数: 21374 | 27 今天看这个,觉得放射性元素辐射这个东西实在可怕
http://www.youtube.com/watch?v=2G422z8pDn4
This is the sequence of the nuclear criticality accident from "Fat Man and
Little Boy" with John Cusack. This storyline was based on two real accidents
that occurred early in the US's nuclear weapons program that killed two
brilliant scientists.
What happened was that he was checking the neutron flux, keeping the two
halves of the plutonium core seperated with a screwdriver. The blocks around
the sphere are tungsten carbide, a ne... 阅读全帖 |
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b*****d 发帖数: 7166 | 28 【 以下文字转载自 TrustInJesus 讨论区 】
发信人: JasonYang (Jason), 信区: TrustInJesus
标 题: Re: “圣经”大智慧:强奸处女应该受到什么样的惩罚?
发信站: BBS 未名空间站 (Sun Aug 18 01:27:47 2013, 美东)
I am not trying to prove anything. I just show how I understand this passage
.
Talking about marriage, I believe it is not good to divorce. So it is good
that one man and one women after sex should not seperate (become one flesh).
They are supposed to marry first and then have sex. People need to be very
very serious about sex.
If we have an agreem... 阅读全帖 |
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d*b 发帖数: 4453 | 30 难道不是越南盾?对了,还是 married owned seperately,啥意思? 要是放在我名下
该多好。 |
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c*********9 发帖数: 229 | 31 long term, i think railway is the solution for both passenger and cargo
trasportation. it is faster and cheaper than airway. however, expect
huge push back. USA wont vote for it as its mainland is seperated by two
big oceans. |
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d*********2 发帖数: 48111 | 32 你这保险太牛鼻了。
我们现在老板提供的最好的保险, 头3次免copay. no deductible.
员工每月$450(老板承担了70%), 家属一个要$900+了
如果家里2,3个孩子的, 买了这保险, 基本上税后就全交待了
就这样也做不到$50生个孩子。 10个月例行检查超过10次, 还要约营养师,
down syndrome遗传学专科等等。
我们当初顺产全套下来的自付$3000左右。 主要被医院坑了, 医生和医院seperate
bill. 医生的保险基本都cover了, 医院付了小1000。 |
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a*******a 发帖数: 4233 | 33 千老再没个保险可以去跳楼了。。
seperate |
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j****i 发帖数: 68152 | 34 标 题: 我(eric zhou)再整理一遍关于的这次的所有帖子
发信站: BBS 未名空间站 (Thu Mar 1 02:20:30 2007)
防止有人用内容无关的eric zhou标题刷版。想搞清真相的,请只re这个帖子。
0)她从五月提出离婚诉讼,就一直有律师在后面指挥。每一步都是有预谋的。
1) 我到加州前我们的共同存款(扣除creditcard debt)实际不到一万。上学的时候,
我们每个假期都出去旅游,还带着她父亲在西部各州开车长途旅游。她
2) 即使这笔钱全是ex存的,然后ex把她以后一年的税后收入全部存起来,她也只攒了2
万五。所以所谓我们的6万存款是她的奖学金攒的,完全是无稽之谈。她到2006年5月只
拿了3年半RA的工资。
3) 我提出离婚后,在2006年7月付给ex $10000现金,给她付了$9000的creditcard
balance,给她买了$900的笔记本。8月给她买了价值$2000的钻戒和项链。从提出离婚
到今天,我一直在给她付她的医疗保险,汽车保险和手机费(每月共约$230)。
4) 我从第一天,就告诉她6万全归她。事实上,我给她的协议,现金加所... 阅读全帖 |
|
|
j****i 发帖数: 68152 | 36 标 题: 我(eric zhou)再整理一遍关于的这次的所有帖子
发信站: BBS 未名空间站 (Thu Mar 1 02:20:30 2007)
防止有人用内容无关的eric zhou标题刷版。想搞清真相的,请只re这个帖子。
0)她从五月提出离婚诉讼,就一直有律师在后面指挥。每一步都是有预谋的。
1) 我到加州前我们的共同存款(扣除creditcard debt)实际不到一万。上学的时候,
我们每个假期都出去旅游,还带着她父亲在西部各州开车长途旅游。她
2) 即使这笔钱全是ex存的,然后ex把她以后一年的税后收入全部存起来,她也只攒了2
万五。所以所谓我们的6万存款是她的奖学金攒的,完全是无稽之谈。她到2006年5月只
拿了3年半RA的工资。
3) 我提出离婚后,在2006年7月付给ex $10000现金,给她付了$9000的creditcard
balance,给她买了$900的笔记本。8月给她买了价值$2000的钻戒和项链。从提出离婚
到今天,我一直在给她付她的医疗保险,汽车保险和手机费(每月共约$230)。
4) 我从第一天,就告诉她6万全归她。事实上,我给她的协议,现金加所... 阅读全帖 |
|
j****i 发帖数: 68152 | 37 标 题: 我(eric zhou)再整理一遍关于的这次的所有帖子
发信站: BBS 未名空间站 (Thu Mar 1 02:20:30 2007)
防止有人用内容无关的eric zhou标题刷版。想搞清真相的,请只re这个帖子。
0)她从五月提出离婚诉讼,就一直有律师在后面指挥。每一步都是有预谋的。
1) 我到加州前我们的共同存款(扣除creditcard debt)实际不到一万。上学的时候,
我们每个假期都出去旅游,还带着她父亲在西部各州开车长途旅游。她
2) 即使这笔钱全是ex存的,然后ex把她以后一年的税后收入全部存起来,她也只攒了2
万五。所以所谓我们的6万存款是她的奖学金攒的,完全是无稽之谈。她到2006年5月只
拿了3年半RA的工资。
3) 我提出离婚后,在2006年7月付给ex $10000现金,给她付了$9000的creditcard
balance,给她买了$900的笔记本。8月给她买了价值$2000的钻戒和项链。从提出离婚
到今天,我一直在给她付她的医疗保险,汽车保险和手机费(每月共约$230)。
4) 我从第一天,就告诉她6万全归她。事实上,我给她的协议,现金加所... 阅读全帖 |
|
j****i 发帖数: 68152 | 38 摘要:
我从中学到现在,都叫eric, 从来没有一天,我会想到,我会羞于告诉别人我的名字叫
eric. 我现在不是证明我是一个完美的圣人,只是要下一次可以自信的告诉任何一个人
,我的名字叫eric.
<千与千寻>里面白龙对千寻说的一句话,我永远记得,“他们要夺走你的名字。你一定
不要忘记自己的名字。失去了你的名字,你就再也会不去了。
标 题: 我(eric zhou)再整理一遍关于的这次的所有帖子
发信站: BBS 未名空间站 (Thu Mar 1 02:20:30 2007)
防止有人用内容无关的eric zhou标题刷版。想搞清真相的,请只re这个帖子。
0)她从五月提出离婚诉讼,就一直有律师在后面指挥。每一步都是有预谋的。
1) 我到加州前我们的共同存款(扣除creditcard debt)实际不到一万。上学的时候,
我们每个假期都出去旅游,还带着她父亲在西部各州开车长途旅游。她
2) 即使这笔钱全是ex存的,然后ex把她以后一年的税后收入全部存起来,她也只攒了2
万五。所以所谓我们的6万存款是她的奖学金攒的,完全是无稽之谈。她到2006年5月只
拿了3年半RA的工资。
3) 我... 阅读全帖 |
|
f******t 发帖数: 19544 | 39 Maverick现在奥运版当板斧的吧,这么早就在买买提参与是非了。
回答Maverick的问题
我2006年12月征友时,已经和她legally seperate 3个月了。这不是按异地工作算起,
而是按照她file petition, 我counter petition算起。我异地工作,到那时已经1年半
了。 |
|
j****i 发帖数: 68152 | 40 摘要:
我从中学到现在,都叫eric, 从来没有一天,我会想到,我会羞于告诉别人我的名字叫
eric. 我现在不是证明我是一个完美的圣人,只是要下一次可以自信的告诉任何一个人
,我的名字叫eric.
<千与千寻>里面白龙对千寻说的一句话,我永远记得,“他们要夺走你的名字。你一定
不要忘记自己的名字。失去了你的名字,你就再也会不去了。
标 题: 我(eric zhou)再整理一遍关于的这次的所有帖子
发信站: BBS 未名空间站 (Thu Mar 1 02:20:30 2007)
防止有人用内容无关的eric zhou标题刷版。想搞清真相的,请只re这个帖子。
0)她从五月提出离婚诉讼,就一直有律师在后面指挥。每一步都是有预谋的。
1) 我到加州前我们的共同存款(扣除creditcard debt)实际不到一万。上学的时候,
我们每个假期都出去旅游,还带着她父亲在西部各州开车长途旅游。她
2) 即使这笔钱全是ex存的,然后ex把她以后一年的税后收入全部存起来,她也只攒了2
万五。所以所谓我们的6万存款是她的奖学金攒的,完全是无稽之谈。她到2006年5月只
拿了3年半RA的工资。
3) 我... 阅读全帖 |
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f******t 发帖数: 19544 | 41 Maverick现在奥运版当板斧的吧,这么早就在买买提参与是非了。
回答Maverick的问题
我2006年12月征友时,已经和她legally seperate 3个月了。这不是按异地工作算起,
而是按照她file petition, 我counter petition算起。我异地工作,到那时已经1年半
了。 |
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b*********3 发帖数: 1709 | 42 I think empires are inherently unstable. The hegemon is not going to rule
other lands out of some desire for selfless good-doing; it will seek a
profit. The vassal states will resist this. This will inevitably result in
pulling apart. Victoria was named Empress in 1879; Ireland was seperated in
1922, and other colonies followed. France lost Vietnam and Algeria. The USSR
lasted sixty years. If we wish to lead the world, we should seek a
different model. Personally, aggressive leadership in scienc... 阅读全帖 |
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g*********e 发帖数: 458 | 43 does this line seperate the sea between japan and taiwan properly? |
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g*********e 发帖数: 458 | 44 Does the line seperate Taiwan from Japan fairly?
It's fine for Japan to have Diaoyu Island. If only Taiwan and its
surrounding sea are not in Japan's control, then for me, it's fine. |
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a***n 发帖数: 2632 | 45 我想,如果这是在中国,你说的新关系新手段的方法可能更有市场。可这是说在美
国。seperated is not equal这种观念已经深入人心。而且从来就是走议会和选举
的路线。那种中国的不直接冲突,打擦边球,钻空子的方法是不可能的。
我想这就是我的回答吧。 |
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a***n 发帖数: 2632 | 46 seperated is not equal在所有的civil rights (黑人,妇女)运动中就有了。这
不是什么新发明。
如果你要在中国人的同性恋圈子里,只要求和婚姻一样的权力和义务,但只属一个
civil union的名字,大部分人会同意的。但我说了,这是在美国。这条路走不通。
国情如此。
事。 |
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s***6 发帖数: 5749 | 47 来自主题: RuralChina版 - 傻妞的眼泪 My neighbour is driving, I will pay gasoline, we rented 2 seperate rooms,
but we always play and dining together. We went to the top of bernal fall
today, it is amazing. |
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h**e 发帖数: 9290 | 48 3k should seperate the bbs from other business so it will not be affected by
TG's daily activities. |
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h**e 发帖数: 9290 | 49 3k should seperate the bbs from other business so it will not be affected by
TG's daily activities. |
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m******1 发帖数: 19713 | 50 婚姻作为一个法律概念,主要的目的是给相爱的人特权及保护,这里说的相爱指的是爱
情,不是指的亲人之间的爱。既然你前边都说了支持给同性伴侣和异性伴侣一模一样的
BENEFIT,就说明你承认相爱的人之间的这种特权保护是多么的重要,既然如此,你为
什么那么在乎这个名称呢?我们在乎是因为,在美国的人权斗争史上,seperate is
not equal是个政治共识。 |
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