s*********t
发帖数: 4253 | 1 在此我们想告诉大家。我们已回复这位嘁哩喀喳同学好多遍了。如果你们有任何疑问不
妨和我们分享。
问：照“圣经”，狮子老虎所有的走兽以及飞鸟现在都是吃草的，你真的相信吗？
答：今天连熊都还有吃草的，在世界之初还有什么不可能的事情呢？
NOTE: 熊猫也是走兽吧！
https://en.wikipedia.org/wiki/Giant_panda
The giant panda also known as panda bear or simply panda, is a bear[3]
native to south central China.[1] It is easily recognized by the large,
distinctive black patches around its eyes, over the ears, and across its
round body. The name "giant panda" is sometimes used to distinguish it from
the unrelated red panda. Though it b... 阅读全帖 |
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s*********t
发帖数: 4253 | 2 在此我们想告诉大家。我们已回复这位嘁哩喀喳同学好多遍了。如果你们有任何疑问不
妨和我们分享。
比如。。。
问：照“圣经”，狮子老虎所有的走兽以及飞鸟现在都是吃草的，你真的相信吗？
答：今天连熊都还有吃草的，在世界之初还有什么不可能的事情呢？
NOTE: 熊猫（吃素的熊）也是走兽啊
https://en.wikipedia.org/wiki/Giant_panda
The giant panda also known as panda bear or simply panda, is a bear[3]
native to south central China.[1] It is easily recognized by the large,
distinctive black patches around its eyes, over the ears, and across its
round body. The name "giant panda" is sometimes used to distinguish it from
the unrelated red panda. ... 阅读全帖 |
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D***e
发帖数: 48486 | 3 1. Wood Destroying Insect Inspection
2. General Insect/Pest/rodent Inspection
3. Water Potability (bacteriological only) test
一共75枚，我先列三吧？ |
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D*******y
发帖数: 4252 | 4 3. 水饮用度检查一般都不包括, 反正是公共上下水，好象一般都不查
2. Insect/Pest/rodent Inspection一般我们这里是有专门的公司来做，Termite
inspection也是其中一个
1. Wood Destroying Insect Inspection也是跟2一起做的 |
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m**********e
发帖数: 2808 | 5 Diet of a Panda:
The giant panda's diet consists mainly (over 99% (Schaller et al. 1985)) of
bamboo shoots, up to 13 mm (1/2") in diameter, and bamboo roots. It also
eats bulbs of plants such as iris and crocus, grasses and occasionally fish,
insects, carrion, eggs and small rodents.
Estimated BMI of a Panda*: 31.836
@#^%#&$%#&&@#????!!!!!
*Note: According to this, I chose the mid point of length (175cm), and mid
point of weight range (97.5kg). |
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k**L
发帖数: 3630 | 6 有. "pigeon is rodent with wings." 基本上鸽子全身都是病菌.特别是城市里的鸽子
.因为环境污染.鸽子身上都沾满了空气中的污染物,它们在街道的积水里洗澡只能使身
上的病菌更多.它们会到处的吃垃圾.身上带着大量的病菌.它们的粪便不但腐蚀性强,细
菌量也是很惊人的. 你说是不是和老鼠差不多? |
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r****t
发帖数: 9 | 8 wuwu....
Never succeeded in the installation of Oracle 8i for LInux, using Redhat 6.2.
NUmerous version of JDK used, 1.1.6, 1.1.8, even 1.2.2
If I use oracle/dba account, the installation program will crash (core dump)
at the last stage; Installation can complete if I use root/root, but cannot
create database instance.
who can help me please!
Rodent Chou Chou |
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s*r
发帖数: 2757 | 9 不知道这个背后是不是有mosanto的funding
http://www.pnas.org/content/early/2010/02/26/0912955107.short?rss=1
“Control” laboratory rodents are metabolically morbid: Why it matters
Abstract
Failure to recognize that many standard control rats and mice used in
biomedical research are sedentary, obese, glucose intolerant, and on a
trajectory to premature death may confound data interpretation and outcomes
of human studies. Fundamental aspects of cellular physiology, vulnerability
to oxidative stress, inflammation |
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L*****C
发帖数: 157 | 10 A lot of people doing rodent behavior work are not well trained in this area
. They just pick up a task and run a standard protocol. To do good behavior
work, you need people trained in psychology (a branch called behavioral
neuroscience), not molecular or cellular biology. |
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g******t
发帖数: 20 | 11 HPLC, electrophysiology, behavioral pharmacology in primates and rodents.
and ... |
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t*d
发帖数: 1290 | 12 前几天在‘制药’版问了个旧药新用的问题（见
http://www.mitbbs.com/mitbbs_article_t.php?board=Pharmaceutical&gid=31176379
）
今天 Nature 的 Editor 针对我的问题特地写了一片 Editorial：
Change of purpose
Nature 465, 267–268 Date published: (20 May 2010)
In 2007, a paper in the journal Cancer Cell announced that the compound
dichloroacetate (DCA) had been found to shrink tumours in rats (S. Bonnet et
al. Cancer Cell 11, 37–51; 2007). That news by itself would not have
created much of a stir: many compounds tested in rodents rais |
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C***k
发帖数: 1570 | 13 3x very much!
J Neuropathol Exp Neurol. 1998 Oct;57(10):915-30.
Expression of JE (monocyte chemoattractant protein-1) is induced by sciatic
axotomy in wild type rodents but not in C57BL/Wld(s) mice.
Carroll SL, Frohnert PW.
Department of Pathology, The University of Alabama at Birmingham, 35294-0007
, USA. |
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f******u
发帖数: 9 | 14 GENERAL SUMMARY
A postdoctoral position is available immediately in the laboratory of Dr.
Christopher Bartlett in the Battelle Center for Mathematical Medicine to
perform neurogenetic studies correlating genetic variation to gene
expression phenotypes from rodent and human brain tissue samples. Essential
methodologies include using multiplex PCR, RT-PCR, quantitative PCR, tissue
histology, in situ hybridization and immunohistochemistry methods interfaced
microscopy data collection. Experience wi |
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D*a
发帖数: 6830 | 15 Moderate caloric restriction initiated in rodents during adulthood sustains
function of the female reproductive axis into advanced chronological age.
Selesniemi K, Lee HJ, Tilly JL.
Aging Cell. 2008 Oct;7(5):622-9. Epub 2008 Jul 24.
多谢!
x********[email protected] |
|
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p*****m
发帖数: 7030 | 17 这个当然不一样了 没人严肃的去用fly搞emotion 搞fear conditioning 搞context le
arning 搞cognitive task呀 无非就是做做很简单的行为的genetics，用rodents大家肯
定是想做更复杂的东西了 这时候rat的优势就有了 而且还很大
举个例子吧 你要教mouse学会来一个CS之后忍住几秒钟才能得到吃的，mouse基本是学不
会的，rat就学的会；还有，go/no-go test小鼠是干不了的 大鼠就没问题，等等等等
例子很多 |
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p*****m
发帖数: 7030 | 18 这个没那么绝对吧 就说rodent，limbic system作出的decision很多时候可以overcome
neocortex，比如说stress/risk response，反过来neocortex又可以影响这些respons
e的强度
往低级了说 疼痛反射也不沾大脑的边 spinal cord最多加上brainstem就搞定了 同时n
eocortex可以overcome低级核团的decision
brain |
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o********r
发帖数: 775 | 19 Review
Many different tumor types have polyclonal tumor origin: Evidence and
implications
Barbara L. ParsonsCorresponding Author Contact Information, a, E-mail The
Corresponding Author
aDivision of Genetic and Reproductive Toxicology, National Center for
Toxicological Research, HFT-120, 3900 NCTR Road, USFDA, Jefferson, AR 72079,
United States
Received 7 February 2008;
revised 14 May 2008;
accepted 15 May 2008.
Available online 31 May 2008.
Abstract
Few ideas have gained such strong acceptance i... 阅读全帖 |
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s******y
发帖数: 28562 | 20 http://www.sciencemag.org/cgi/content/abstract/science.1195298
Published Online October 28, 2010
Science DOI: 10.1126/science.1195298
Science Express Index
Reports
Calcium-Permeable AMPA Receptor Dynamics Mediate Fear Memory Erasure
Roger L. Clem and Richard L. Huganir*
Traumatic fear memories can be inhibited by behavioral therapy or extinction
training in rodent models, but are prone to relapse. Under some conditions,
however, these treatments generate a permanent effect on behavior
sugges... 阅读全帖 |
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p*****m
发帖数: 7030 | 21 在果蝇界内部还行吧 但是代谢领域基本还是rodent的天下。做出来的东西别人买不买
帐不好说。 比方说这个ILP的问题就
是没法区分在development和metabolism里的作用。你随便操作一下ILP 果蝇发育就会
受影响。这样做出来的东西我是不
太放心的反正。
好玩的文章当然是不少 这几年我看到的也许是最重要的东西是slimfat 好像是第一个
发现的amino acid transporter? 在果
蝇里搞出来的。如果是真的的话会很重要，因为这个东东可能是mTOR上游最重要的未知
因子了。不过做的还不够现在 原
因可以参考上面 呵呵 |
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h*******o
发帖数: 4884 | 22 There are a few possibilities, depends on your area of interest
For me, I would check their glucose level. As rodents reliy heavily on
ketone bodies rather than glucose during suckling stage, once they are weaned they would have to
convert to a glucose-dependent phenotype.
If somehow your KO mice have defect in glucose metabolism, they will die.
And they technically die of starvation.
Just a hypothesis. If that hits your case, it will be interesting. |
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p*****m
发帖数: 7030 | 23 evo-devo我也觉得挺有意思 不过这个为什么一定要在fish里做呢？现在在fly和rodent
里做就不错呀 |
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y**********o
发帖数: 37 | 24 最近老板忽然对Alzheimer's disease产生极大的兴趣,非要把课题往这个方向上靠.我
研究了几篇综述后,有些最基本的问题想请教一下版上做这个方向的高人.相信这里应该
有很多前辈能够指导一下我这个外行,晚辈先在这里感谢一下所有回帖的.
问题一,目前市面上各种mouse models似乎都是转了human mutated genes,像APP,PS1什
么的.最常见的检测指标是A-beta和NFT的染色. 老鼠也有内源性的APP和Tau, 好象内源
性的Tau所形成的NFT在小鼠中一直无法诱导,那么内源性的APP可以经诱导产生A-beta斑
块吗? 公司能买到针对rodent的A-beta抗体, 说明有这种可能,只是主流的文章似乎都
在关注human gene product.有没有可能用老鼠内源性的APP来做疾病模型?
问题二,目前主流的观点对这个疾病的机理是否偏向于Ca overload? 我们手头现有的
mouse model显示reduced cytoplasmic Ca concentration,类似PSI/PS2 KO 模型,但这
个似乎无法被牵强的说成Alzhe... 阅读全帖 |
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r*******e
发帖数: 48 | 25 Two postdoctoral positions are available immediately to study
epigenetic modifications on breast cancer. Exciting research
environment, state-of-the-art equipment and facilities are available.
Mandatory requirements: One position is expected to have expertise
in epigenetics, transcription, and genomic approaches (e.g.
microarray, ChIP-chip, ChIP-Seq/RNA-Seq, chromatin remodeling
assays, or global DNA methylation analysis with clinical samples),
cell culture, biochemical and standard molecular as... 阅读全帖 |
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a*****g
发帖数: 543 | 26 luminex
Rules base medicine (now charles river owned) has Rodent and Human cytokine
multiplexing service.
profile |
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n*********1
发帖数: 8 | 27 Hi ,
Can anybody here please tell me where to buy tumor cell lines such as human
melanoma, glioma and non small cell lung cancer cell lines? How do you know
if the cell line has been tested for rodent or human transmissible pathogens
? Will the company provide these documents?
Thanks in advance!
Best |
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n*********1
发帖数: 8 | 28 I got the information from ATCC, but how can we know if the materials have
been tested on rodents or human transmissible pathogens. From the website, i
didn't get it.
Sorry for asking these "stupid" questions. |
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n*********1
发帖数: 8 | 29 多谢楼主了, 我确实不知道从哪查 the materials have been tested on rodents or
human transmissible pathogens. 我需要这些资料.今天下午给公司的technic
support打电话, 可惜他们已下班了. 如果您愿意邦我查, 那真是太感谢了.
三种cell lines:
Glioma: C6(CCL-107)
NSCLC: NCI-H1299(CRL-5803)
Melanoma: B16-F10(CRL-6475)
其实我也不知道哪种更好,更容易接种. 我们的目的是造肿癌模型后,用化疗药物诱导AP
site形成,然后对AP site进行定量的检测. 如果楼主能给予点拨的话,感激不尽!!! |
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h*******o
发帖数: 4884 | 30 打算用drinking water的方式给药
vivaria 现有的drinking bottle太容易漏了,无法测量 water Intake
哪位大虾用过类似的给药方式和好一点的bottle/sipper tube的,麻烦推荐一个
谢谢 |
|
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a********k
发帖数: 2273 | 32 但是你就觉得鱼比rodent有更多贡献？线虫和果蝇的贡献咱暂且不提，鱼到现在基本可
以说是零贡献。。。有了cKO以后有没有零头还是个问题
的零 |
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s******y
发帖数: 28562 | 33 rodent 好歹是个哺乳动物啊。
鱼啊什么的和人也差的太远了
的零 |
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s**u
发帖数: 9035 | 34 Neurological Diseases and Receptor Pharmacology--Post-doctoral position at the NIA
Department of Health and Human Services
National Institutes of Health
National Institute on Aging
The National Institute on Aging (NIA), a major research component of
the National Institutes of Health (NIH) and the Department of Health
and Human Services (DHHS), is recruiting for a postdoctoral research
position in the Receptor Pharmacology Unit of the Laboratory of
N... 阅读全帖 |
|
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i****g
发帖数: 3896 | 36 While the news reports don’t state which drugs Cloony took to beat malaria,
It is most likely that he was treated with artemisinin-based combination
therapies (ACTs), which became available in the late 1990s and are now in
widespread use. If that is the case, he has benefited from mouse studies
done in China the late 1960s and early 1970s when over 100 traditional
herbal remedies were screened in a rodent model of malaria for anti-malarial
activity (1). Eventually “Project 523” scored a hit whe... 阅读全帖 |
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h*******o
发帖数: 4884 | 37 In many of the common species, the reproductive span of male is much longer
than female although life span has the opposite trend.
A lot of the discoveries in yeast or worms or even rodents, cannot and
should not be directly translated into human beings. This is particularly
true since yeast or worms don't have reproductive hormones in the system.
Also, if we have to bring evolution in, I personally think reproductive
senescence is a much more reliable aging marker than simple life span. |
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y******8
发帖数: 1764 | 38 "There can be stages where rodents are in constant estrus/constant diestrus,
etc"
This should be a model to study menopause. I would conduct a screen on this
if possible.
too.
of
, etc |
|
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s**u
发帖数: 9035 | 40 NIH-funded postdoctoral position available immediately in the laboratory of
sensorimotor integration at the University of Maryland School of Medicine
Postdoctoral Fellowship
An NIH-funded postdoctoral position available immediately in the
laboratory of sensorimotor integration at the University of Maryland
School of Medicine http://cortex.umaryland.edu. The research project
descending modulation of pain, and involves extracellular and
intracellular recordings from brain slices, optogenetics an... 阅读全帖 |
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S***a
发帖数: 257 | 41 Methods Mol Biol. 2010;598:173-84.
The sheep erythrocyte T-dependent antibody response (TDAR).
White KL, Musgrove DL, Brown RD.
Methods Mol Biol. 2010;598:159-71.
The T-dependent antibody response to keyhole limpet hemocyanin in rodents.
Plitnick LM, Herzyk DJ.
Pls send to columxia at gmail.com
Thanks a lot! |
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p*****m
发帖数: 7030 | 42 微观来看可能比这个会好一点，因为06年的报告没有预计到这几年基础研究的进步（打
个比方，当时他们调查中国居然只有3个地方能做transgenic rodent)，还有政府投入
的增加。宏观来看可能要比这个悲观一点，因为得加上经济硬着陆的风险。PS 看起来
咨询公司也不是完全的BS, lol |
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t*****z
发帖数: 1598 | 43 打扰了！我想找这篇文章。请发我邮箱t*****[email protected]。祝新学期愉快！
http://www.ncbi.nlm.nih.gov/pubmed/22906797
Parasitology. 2012 Aug 20:1-8. [Epub ahead of print]
Similarities among ectoparasite fauna of sigmodontine rodents: phylogenetic
and geographical influences.
Cruz LD, Fernandes FR, Linhares AX.
PMID: 22906797 |
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c******a
发帖数: 267 | 44 Pls send to columxia at gmail.com
Thank you very much!
Curr Protoc Toxicol. 2003 Aug;Chapter 13:Unit13.5.
In vivo assessment of prenatal developmental toxicity in rodents.
Narotsky MG, Kavlock RJ.
Source
U.S. Environmental Protection Agency, Research Triangle Park, North Carolina
. |
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s******y
发帖数: 28562 | 45 版上火药味好浓啊，大家看看文章散散心吧。
新出的文章认为actin 的总体动态以及相关的serum response factor 是一个生理时钟。
Cell. 2013 Jan 31;152(3):492-503. doi: 10.1016/j.cell.2012.12.027.
Blood-borne circadian signal stimulates daily oscillations in actin dynamics
and SRF activity.
Gerber A, Esnault C, Aubert G, Treisman R, Pralong F, Schibler U.
Source
Department of Molecular Biology, Sciences III, University of Geneva, and
National Centre of Competence in Research Frontiers in Genetics, 1211 Geneva
, Switzerland.
Abstract
In peripheral ti... 阅读全帖 |
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f******g
发帖数: 1003 | 46 http://www.merckmanuals.com/vet/pharmacology/anti-inflammatory_
&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Biochemical mediators released during inflammation intensify and propagate
the inflammatory response (see Anti-Inflammatory Agents: Actions of
Inflammatory MediatorsTables). These mediators are soluble, diffusible
molecules that can act locally and systemically. Mediators derived from
plasma include complement and complement-derived peptides and kinins.
Released via the classic or alte... 阅读全帖 |
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l**********1
发帖数: 5204 | 47 why select homo sapiens to observe homology of the gene,
the expression system is Yeast,
the original of that gene is from rodents or other mammals ?
|
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m******g
发帖数: 467 | 48 Science 14.05.02
推荐：
1. Reconstructing the DNA Methylation Maps of the Neandertal and the
Denisovan
小知识：
1. Male Scent May Compromise Biomedical Studies
The mice that didn't show pain had been handled by male students
Rodents exposed to male odors were actually feeling less pain
2. Therapeutic Cloning Reaches Milestone
Two teams have independently derived human embryonic stem cells
By cloning adult skin cells
3. Genomic Signatures of Specialized Metabolism in Plants
How algae and land plants ha... 阅读全帖 |
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A******y
发帖数: 2041 | 49 For rodents? Yes. For human could be very hard. For example, when you are
doing siRNA or short DNA injection in mice or rat, you can get the
nucleotides into the tissues without doing anything. |
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M******n
发帖数: 29 | 50 目前，postdoc，发表的论文有10，在投的4。已经有过几篇独立审稿经验。
phd 主要方向Alzheimer's disease, anxiety and depression, aging, ApoE,
melatonin.
Postdoc 主要方向 CNS regulation in metabolism.
擅长 rodent Behavior，也有过临床调查的背景。
谢谢大家的关注和推荐。 |
|
