K******S 发帖数: 10109 | 1 not surprised. It's been called taco hell for long time and it's been
associated with diarrhea for long time as well. hehe
dusting
phosphate,
20110125,1,2421413.story |
|
G****o 发帖数: 124 | 2 嗯,你说得对。
大家再看看这些吧:
Nucleic Acids In Nutrition
Unlike the other large biological molecules -- proteins, fats, and
carbohydrates -- you don't need to eat nucleic acids in order to make
nucleic acids. Instead, you make your nitrogenous bases from amino acids,
which are the building blocks of protein. You make the sugars in DNA and RNA
from sugars you ingest when you eat carbohydrates, and you recycle
phosphates from a variety of other sources. Nucleic acids don't contain any
calories, and don't prov... 阅读全帖 |
|
n**e 发帖数: 2026 | 3 根据央视《朱令十二年》电视记录片,朱令最终在4月28日通过铊检测作出铊中毒的诊
断。这个过程完全是贝志城鼎力推动而完成的。而贝志城之所以先后通过两次分别通过
朱令的父亲和母亲寻求铊中毒检测,根据的是国外专家回复的电子邮件诊断为铊中毒。
贝:十号发了那是个周一,周三我就给朱令他爸爸打电话说,我看到提问里还有说是铊
中毒。
贝志城:很多医生很激烈地发表意见,说一定要作这个化验,因为她的症状太像铊中
毒了。
朱令父亲:贝志城给我打了个电话,说有人觉得是不是就是铊中毒。
朱令母亲:他(贝志城)说,阿姨你是怎么了,这么多人都说是铊中毒,你为什么就
不去化验。
尼罗河与多位直接参与朱令远程诊断的美国医师通过电邮电话和传真讨论过当时诊断铊
中毒的情况。他们中有人还保存有当时的电邮文件。在贝志城和他的同伴们得到这些邮
件的同时,这些邮件也传给了在美国热心帮助朱令的医生们,而且这些医生们相互之间
也通过电子邮件交换看法。根据他们的回忆,当时提出了很多很多可能的诊断,而且相
当大的一部分是愚蠢的想法。提供诊断意见的邮件如潮水一般涌来,我们几乎被这些冗
长的邮件淹没,根本不要指望看到“铊中毒”从各种其他鉴别... 阅读全帖 |
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w******e 发帖数: 953 | 4 How safe does transgenic food need to be?
Laura DeFrancesco1
Journal name:
Nature Biotechnology
Volume:
31,
Pages:
794–802
Year published:
(2013)
DOI:
doi:10.1038/nbt.2686
Published online 10 September 2013 Corrected online 26 September 2013
Article tools
PDF
How safe does transgenic food need to be?
Download as PDF (812 KB) View interactive PDF in ReadCube
Citation
Reprints
Rights & permissions
Article metrics
Disputes over how to assess a foodstuff's safety continue to play into
public fears a... 阅读全帖 |
|
q**x 发帖数: 1636 | 5 Material Safety Data Sheet
Sulfuric Acid
ACC# 22350
Section 1 - Chemical Product and Company Identification
MSDS Name: Sulfuric Acid
Catalog Numbers: S71211SC, S71826, S79200, S80213, S80213-1, A298 212,
A298212, A300 212, A300 225LB, A300 500, A300 612GAL, A300 700LB, A300212,
A300225LB, A300500, A300612GAL, A300700LB, A300C 212, A300C212, A300C212001,
A300C212002, A300C212003, A300C212004, A300C212005, A300C212006,
A300C212007, A300C212008, A300C212009, A300C212010, A300C212LC, A300FP 500,
A30... 阅读全帖 |
|
q**x 发帖数: 1636 | 6 Material Safety Data Sheet
Sulfuric Acid
ACC# 22350
Section 1 - Chemical Product and Company Identification
MSDS Name: Sulfuric Acid
Catalog Numbers: S71211SC, S71826, S79200, S80213, S80213-1, A298 212,
A298212, A300 212, A300 225LB, A300 500, A300 612GAL, A300 700LB, A300212,
A300225LB, A300500, A300612GAL, A300700LB, A300C 212, A300C212, A300C212001,
A300C212002, A300C212003, A300C212004, A300C212005, A300C212006,
A300C212007, A300C212008, A300C212009, A300C212010, A300C212LC, A300FP 500,
A30... 阅读全帖 |
|
|
x********u 发帖数: 15396 | 8 这个钙磷比问题我刚开始喂raw的时候也问过。不过我担心的是钙不够,lol..在查资料过程中,我有看到一个理论是说光看有多少钙没有用,因为不是所有
钙的化合物都很容易被吸收,化合物有calcium carbonate, calcium citrate, etc.容
易被狗吸收的是calcium phosphate。也是骨头里面的"主要"成分.
这个10%骨头其实不是根据狗粮推荐的钙磷比算出来的。而是因为一般的prey身体组成
是5-10% organs,10-15% edible bones,80-85% muscle meat (and the rest of the
critter。这些生物的体内钙磷比又是最均衡的(差不多1:1),所以才会有8:1:1的说法。 |
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h**l 发帖数: 4883 | 9 如果鸡的骨头就有30%以上,那prey里为什么只有10%. 当然我不明白什么比例的骨头是
edible的.不过不吃不edible的骨头, 那不是没有吃到prey完全的均衡营养成分了么.
Calcium phosphate里钙磷元素molar比例是3:2,这个没法balance钙磷比,所以还是要看
多少其他钙化合物能被吸收了.
我有看到一个理论是说光看有多少钙没有用,因为不是所有
the
法。 |
|
x********u 发帖数: 15396 | 10 钙磷本来就不是光靠Calcium phosphate来平衡的阿
那个网站显示的30%是拔了毛,放了血,掏了内脏,切了头脚的鸡。和whole prey还是
有区别的。 |
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m***j 发帖数: 9290 | 11 两种狗粮成分如下
1。Beef, Beef Lung, Beef Liver, Wheat Flour, Egg Product, Flax Seed, Salt,
Dicalcium Phosphate, Calcium Chloride, Sunflower Oil, Dextrose, Citric Acid,
Potassium Chloride, Choline Chloride, Rosemary, Cinnamon, Garlic, DL-Alpha
Tocopherol Acetate, Iron Sulfate, Selenium Supplement, Zinc Oxide, Copper
Sulfate, Vitamin B12 Supplement, Niacin, Calcium Pantothenate, Manganous
Oxide, Vitamin A Acetate, Thiamine Mononitrate, Riboflavin Supplement, D-
Activated Animal Sterol (source of vitamin D |
|
m***j 发帖数: 9290 | 12 再来一种,就更便宜了。
3。Beef Hearts, Beef Kidneys, Beef Lungs, Whole Wheat Flour, Brown Rice,
Wheat Bran, Beef Fat (preserved with Mixed Tocopherols and Citric Acid),
Sucrose, Flaxseed Meal, Calcium Carbonate, Sodium Chloride, Modified Food
Starch, Sodium Phosphates, Water, Brewer's Yeast, Dried Kelp Meal, Lecithin,
Natural Smoke Flavor, Sorbic Acid, Glycerin, Rosemary Extract, Canola Oil,
Choline Chloride Supplement, Ferrous Sulfate, Riboflaven Supplement, Vitamin
B-12 Supplement, Vitamin A Acetate, Foli |
|
p***7 发帖数: 449 | 13 发现好像两只猫discipline起来比一只要难得多?Asher今天干坏事,我就把他关进厕
所15分钟反
省,结果Talyn跑过去跟他在门底下玩躲猫猫(就是两边互伸爪子玩),然后小家伙出
来什么事都没
有。。。那那那。。。这time-out白弄了?
另外再问问猫粮(好像我老问这个)。Blue Buffalo kitten + Evo的混吃法结束,现
在两猫开始
吃纯Evo。可是我又开始担心Evo据说的高phosphate level(好像是对kidney不好的猫
不好),所
以又开始找了。这个Nature's Variety Instinct怎么样?有什么东西偏高吗?不然我
就给他们吃
这个了。 |
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x********u 发帖数: 15396 | 14 natural choice:
Lamb meal, ground rice, rice flour, rice bran, sunflower oil (preserved with
mixed tocopherols, a source of natural vitamin E), poultry fat (preserved
with mixed tocopherols, a source of natural vitamin E), natural flavors,
rice gluten, dried egg product, dried beet pulp (sugar removed), potassium
chloride, l-lysine, dried kelp (source of iodine), salt, choline chloride,
zinc sulfate, vitamin E supplement, taurine, ferrous sulfate, ascorbic acid
(source of vitamin C), biotin, cop... 阅读全帖 |
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x********u 发帖数: 15396 | 15 加过东西的肉,人吃没事,猫狗比较敏感, 会拉肚子或者狂喝水
Typically, enhanced meat is simply a cut of meat that has been injected with
a water solution that contains salt and sodium phosphate, a solution that
is supposed to add both flavor and moisture to leaner cuts of meat. Such
enhanced meat products are labeled accordingly and often bare words similar
to “enhanced with up to 10% of a solution.” According to the FDA, meat
animals are bred to be leaner than in years past and the addition of a
solution, which constitutes ... 阅读全帖 |
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x********u 发帖数: 15396 | 16 这个成分很烂呀
Ground pea, venison meal, pea protein, coprah oil, natural flavors, venison,
soya oil, sodium bisulfate, anchovy oil (source of EPA/DHA), monocalcium
phosphate, DL-methionine, salt, taurine, potassium chloride, choline
chloride, Vitamins [DL-alpha tocopherol acetate (source of vitamin E),
inositol, niacin supplement, L-ascorbyl-2-polyphosphate (source of vitamin C
), D-calcium pantothenate, biotin, pyridoxine hydrochloride (vitamin B6),
riboflavin supplement (vitamin B2), thiamine mononi... 阅读全帖 |
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v**e 发帖数: 367 | 17 提个醒:Halo Liv-a-Littles treat含有sodium phosphate,有urinary tract
infection的男猫最好别吃。
50 |
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p********f 发帖数: 5148 | 18 Have you tried Merrick turducken?
Crude Protein (Min.) 10.00%
Crude Fat (Min.) 3.00%
Crude Fiber (Max.) 1.5%
Moisture (Max.) 78.00%
Ash (Max.) 1.80%
Magnesium (Max.) .025%
Taurine (Min.) .08%
Ingredients:
Turkey, Chicken, Chicken Broth, Duck, Fresh Sweet Potatoes, Fresh Carrots,
Fresh Snow Peas, Fresh Golden Delicious Apples, Potato Starch-Modified,
Dried Cranberry, Olive Oil, Flax Seed Oil (For Omega -3), Cassia Gum,
Carra... 阅读全帖 |
|
x********u 发帖数: 15396 | 19 我看了一下这个的配方,感觉拉5次正常,因为肉太少,都是些不吸收的东西
Sweet Potatoes, Venison, Potato Protein, Pea Protein, Canola Oil, Dicalcium
Phosphate, Potato Fiber, Flaxseed, Natural Flavor, Calcium Carbonate, Sodium
Chloride, Choline Chloride, Taurine, Natural Mixed Tocopherols, Vitamin E
Supplement, Iron Proteinate, Zinc Proteinate, Copper Proteinate, Ferrous
Sulfate, Zinc Sulfate, Copper Sulfate, Potassium Iodide, Thiamine
Mononitrate (Vitamin B-1), Manganese Proteinate, Manganous Oxide, Ascorbic
Acid, Vitamin A Suppleme... 阅读全帖 |
|
i*******p 发帖数: 297 | 20 万能的宠物版啊, 请看看我的情况:
10岁公猫, 6个月前 得过尿道堵塞,吓死了全家人, 目前没有重新发过.
(1)现在是全部吃罐头.
(2)VET叫我们吃science diet, 他不碰.
(3)换来换去, 他喜欢吃friskie 的 鱼罐头, 上面标有 urinary health formula.....
(4)研究后了解到 friskie 罐头质量差, 但是幸运的是尿道病没有复发, 这也是我们现
在的底线了.
(5)现在想换牌子是因为 (A)听说FRISKIE材料差, (B)猫时不时的吐, 估计和A有关系.
经建议, 吃了一圈的 so called "premium" cat food, 发现他最喜欢 - Natural's
balance 的 cat stew formula -Delectable Delights™ Purrrfect Paella&
#8482; Cat Stew Formula:
http://www.naturalbalanceinc.com/resizer.aspx?filename=uploads/images/0129852970... 阅读全帖 |
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R******0 发帖数: 6158 | 21 嗯,fancy feast还是不要吃了,lz之前喂的
Wild Salmon Florentine in a Delicate Sauce
Ingredients: Fish broth, salmon, wheat gluten, liver, meat by-products,
chicken, spinach, corn starch-modified, artificial and natural flavors,
added color (Red 3 and other color), calcium phosphate, salt, soy protein
concentrate, potassium chloride, taurine, choline chloride, Vitamin E
supplement, zinc sulfate, thiamine mononitrate, Vitamin A supplement,
ferrous sulfate, manganese sulfate, pyridoxine hydrochloride, niacin,
Vi... 阅读全帖 |
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R******0 发帖数: 6158 | 22 嗯,fancy feast还是不要吃了,lz之前喂的
Wild Salmon Florentine in a Delicate Sauce
Ingredients: Fish broth, salmon, wheat gluten, liver, meat by-products,
chicken, spinach, corn starch-modified, artificial and natural flavors,
added color (Red 3 and other color), calcium phosphate, salt, soy protein
concentrate, potassium chloride, taurine, choline chloride, Vitamin E
supplement, zinc sulfate, thiamine mononitrate, Vitamin A supplement,
ferrous sulfate, manganese sulfate, pyridoxine hydrochloride, niacin,
Vi... 阅读全帖 |
|
发帖数: 1 | 23 同意。养珊瑚nitrate 要是零。养鱼的话只要低于20到50就可以了。另外对phosphate
还有其他的微量元素都有要求。 |
|
t****x 发帖数: 1650 | 24 有过滤包专除phosphate和nitrate的,不过说到底还是要勤换水 |
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z****u 发帖数: 405 | 25 终于能腾出点时间开始计划已久的海水缸了。
老帖子地址:http://www.mitbbs.com/article_t/pets/32976693.html
准备的器材:
Aqueon 14g tank 20x10x16 inch with homemade glass lid
Aquaclear 50 HOB filter 200GPH
Green Element EVO 18"-22" reef Light 10x3W LED (7 10000K and 3 460 nm LED)
Hydor Koralia Nano Aquarium Circulation Pump, 240GPH.
ViaAqua 100W aquarium heater and thermometer
Aquatic Life 115 Mini Internal Protein Skimmer (有点太大,暂时没用它)
CaribSea South Sea dry Rock 15 Pound
Caribsea aragonite dry sand 20 lbs
1.5” PVC pipe rock ... 阅读全帖 |
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z****u 发帖数: 405 | 26 11/05/2016
Nitrite is still off the chart.
The water evaporation is too fast, had to add distilled water to the tank
everyday. Added a DIY glass lid to the tank to reduce the water evaporation.
pH: 8.0
ammonia: ~0 ppm
nitrite: 5+ppm
nitrate: ---ppm
calcium: ---ppm
phosphate: ---ppm
temperature: 79F
salinity: 1.024
added more ammonia to rise its concentration to ~1 ppm |
|
z****u 发帖数: 405 | 27 11/06/2016
Nitrite started to drop quickly, tank cycling close to the end? So fast (my
fresh water tank took two –three weeks to finish)? Will run for a couple of
more days to confirm.
Added one more dry rock into the tank.
Mixed 5 gallon fresh salt water for water change after the cycling, and the
tank will be ready to host fish (planned a pair of ocellaris clown fish to
start with). The water evaporation was reduced quite a bit with the glass
lid.
pH: 8.0
ammonia: ~0 ppm
nitrite: 0.25-0.5ppm
n... 阅读全帖 |
|
z****u 发帖数: 405 | 28 11/19/2016
前两天终于迎来了两条小丑鱼: Fancy Ocellaris clownfish, 比一般的Ocellaris颜色
要更红一些, 黑条纹更深更宽。外加两只cerith snail 和三只nassarius snail 用作
clean crew.
刚来的时候有点不适应新环境, 吃的比较少, 今天看着基本上放松了很多, 进食也
比较活跃。录像是他们在吃frozen mysis shrimp. 基本上也没有但心中的打架发生,
两条鱼还比较亲近, 现在晚上host在鱼缸的右下角过夜。
Nassarius snail 状态不是很好, 买的时候就有点犹豫。现在只有一只到处跑, 其他
两只从来不动。在等一两天, 不行就只能扔了。
Cerith snail挺有意思的, 一只到处跑, 另一只把自己埋在沙子里一动不动, 把它
挖出来立刻又钻进去。先不管它了, 看它能藏到什么时候。
准备再进两三只 hermit crab, 然后进一些软体珊瑚, 一只 skunk cleaner shrimp,
和一对 yellow rose goby/candy pistol shrimp pair... 阅读全帖 |
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w*********r 发帖数: 3382 | 29 from your mocking attitude and poor choice of terms, i can tell that you don't understand how important this discovery is.
all life forms currently found on earth use exclusively phosphate as the building block for their DNA and so do all metabolism activities.
this discovery shows that this type of bacterium, is different from ALL other life forms on earth and POSSIBLY evolved in and from a different environment.
this discovery DOES NOT say conclusively that there are extraterrestrial life, how... 阅读全帖 |
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s********a 发帖数: 303 | 30 So what? Who says life has to be phosphate based? It was not until the
last century did people discover the bizarre-looking fishes in deep ocean.
Now nobody suspect that they are of earthly origin.
This discovery of arsenic life IS indeed extraordinary, but don't you think
it is too much a "leap of faith" to suggest it is from another planet?
Let's look at the facts:
* It is found in the freaking California, not Mars.
* It somehow prospers on earth. (Think about the chance of an earthly l... 阅读全帖 |
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c*******n 发帖数: 1648 | 31 我无聊,查了一下,这种浓度的硫酸正好用来和phosphate rock反应制造磷酸,然后生
成化肥。这种用途占全球所有硫酸产量过半。说不定可以和化肥工业搭钩。卖给铁厂去
污去锈也是大宗的通途。不过如果附近没有这种厂子,运输成本高的话,只有自己建
acid recovery plant。美国主要是用天然气为能源。的确是耗能。 |
|
b*******g 发帖数: 1309 | 32 1. Chen JY, Bottjer DJ, Davidson EH, et al.
Phosphatized polar lobe-forming embryos from the Precambrian of Southwest
China
SCIENCE 312 (5780): 1644-1646 JUN 16 2006
第一作者南大,第一通讯单位南大
2. Qiu MH, Ren ZF, Che L, et al.
Observation of Feshbach resonances in the F+H-2 -> HF+H reaction
SCIENCE 311 (5766): 1440-1443 MAR 10 2006
第二通讯作者南大化学系的老谢,无数人受其谱学原理的折磨,据说第一通讯作者是他
的学生(没有求证)
3. Ji Q, Luo ZX, Yuan CX, et al.
A swimming mammaliaform from the Middle Jurassic and ecomorphological
diversification of earl |
|
l*****g 发帖数: 2087 | 33 中国科学院院士李家洋
生平简介
李家洋(1956.7-)安徽肥西人。植物分子遗传学家,中国科学院院士,研究员。
中国
科学院副院长、党组成员。1982年毕业于安徽农学院(现安徽农业大学),1991年获美国
布兰代斯(Brandeis)大学博士学位,1991—1994年在美国康乃尔大学汤普逊(Boyce
Th
ompson)植物研究所进行博士后研究工作。历任中国科学院遗传研究所所长助理、所长,
遗传与发育生物学研究所所长。
李家洋主要从事植物分子遗传学研究,他利用模式植物拟南芥与重要粮食作物水稻探
索植物生长发育的调控机理。近年来的主要研究工作包括:采用图位法克隆了水稻分蘖控
制基因MOC1,开拓了水稻分蘖控制分子机理研究的新领域;利用水稻脆秆突变体分离了
B
C1基因,阐述了水稻机械强度的控制机理;通过获得的拟南芥胆碱生物合成突变体,初步
明确了胆碱合成与植物温度敏感雄性不育性的关系;通过图位克隆法分离出导致细胞死亡
的基因MOD1,明确了初级代谢途径的缺陷会导致植物细胞凋亡;利用转基因技术,创制出
色氨酸与吲哚乙酸合成量改变的转基因植物,从而提出植物生长素吲哚乙酸生物合成途径
的新模... 阅读全帖 |
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c**i 发帖数: 6973 | 34 Monster Power. Energy technology: Lithium-ion batteries start to take on the
heavy-duty jobs previously handled by other types of battery.
http://www.economist.com/node/17647673?story_id=17647673
Quote:
"lithium is the lightest metal
"The positive electrodes are often made from lithium iron phosphate. Corvus,
however, uses lithium nickel manganese cobalt (NMC) because it provides a
greater energy density.
Note:
(a) Technology Overview, from Corvus Energy.
http://www.corvus-energy.com/technology.... 阅读全帖 |
|
y***n 发帖数: 99 | 35 Paul Greengard, Laboratory of Molecular and Cellular Science,
Rockefeller University, New York, is rewarded for his discovery
of how dopamine and a number of other transmitters exert their
action in the nervous system. The transmitter first acts on a
receptor on the cell surface. This will trigger a cascade of
reactions that will affect certain "key proteins" that in turn
regulate a variety of functions in the nerve cell. The proteins
become modified as phosphate groups are added (phosphorylatio |
|
l*****k 发帖数: 587 | 36 ??? why I got a different understanding about?
it is because the arrange of the base such that the phosphate backbone are
spaced unevenly, thus, on one side, they are closer, on another side, they
are farther apart. and that's why major and minor.
I can't imagine your way of explanation...
the groove are hydrophobic region, where a lot of protein-DNA binding occurs. |
|
s******y 发帖数: 28562 | 37 That really depends on the chemistry of your reaction and your product.
Tris has free amino group that may quench some reactive group.
Phosphate don't have amino group but it may precipitate with certain ions.
So that really depends on what the reaction really need to have or need to
avoid |
|
p********g 发帖数: 3 | 38 Autophagy. 2010 Apr 15;6(3). [Epub ahead of print]
Regulation of cell death by sphingosine 1-phosphate lyase.
Colié S, Codogno P, Levade T, Andrieu-Abadie N.
Please send the papers to b******[email protected]
Thanks so much!!!! |
|
s****a 发帖数: 436 | 39 Purification of biotinylated proteins on streptavidin resin: A protocol for
quantitative elution。
For elution of biotinylated proteins, 500 mL
release solution (2% SDS, 30 mM biotin, 50 mM phosphate,
100 mM NaCl, 6 M urea, 2 M thiourea, pH ,12)
were added to the resin and incubated for 15 min at RT,
followed by incubation for 15 min at 96C. Afterwards, the
resin was pelleted by centrifugation for 5 min at 16 100g |
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c*********t 发帖数: 340 | 40 Dendreon
They isolate dendritic cells from the patient and stimulate with PAP (
prostate associated phosphate or something, a tumor associated antigen), and
infuse back
the median survival time could be prolonged by four months
then tumor cells expressing low levels of PAP would come up
75K for one round of treatment
really expensive
but it works |
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a***e 发帖数: 1010 | 41 you need a bridge DNA.
check NEB
Taq DNA Ligase catalyzes the formation of a phosphodiester bond between
juxtaposed 5′ phosphate and 3′ hydroxyl termini of two adjacent
oligonucleotides which are hybridized to a complementary target DNA. The
ligation will occur only if the oligonucleotides are perfectly paired to the
complementary target DNA and have no gaps between them; therefore, a single
-base substitution can be detected. Taq DNA Ligase is active at elevated
temperatures (45°C-65°C) (1,2). |
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b******k 发帖数: 1874 | 42 so, if i am going to ligate two tandem short fragments (say, 50bp each):
synthesize 50nt oligo of Sense and antisense (with 5' phosphate group modifi
cation),
anneal them by boiling and cooling naturally,
then ligate the annealed fragments (ideally, with synthesizd 3' overhang fro
m first one, and 5' overhang from 2nd)by conventional T4 ligase system?
thanks. |
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c**a 发帖数: 94 | 43 his tagged 的蛋白用nickel resin纯化出来,在elution buffer里, buffer里有
imidazole, sodium
phosphate. 一共1ml. 以后需要做activity asssy, 请问怎么保存才能不失活性?
是不是要加protease inhibitors, glycerol? 加多少glycerol? 冻-20还是-80? |
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t*********o 发帖数: 20 | 44 1. buffer, potassium phosphate buffer, pH 7.4,用 1XPBS 可以代替吗?还是要用
henderson-hasselbalch equation去配buffer?
2.实验室有570和595nm的plate reader filters. 没有600nm的filter。这样计算时,
能用相应的molar extinction coefficients(600nm)代替595nm的系数吗?会有误差吧
?大吗
3.如果我用fluorescence,560(excitation)/590(emmision), 实验室没有560德filter,
能用530的代替吗?我用530/590,但是得出的数值只有~60,我做cell proliferation
study,觉得很诡异,manual上都是2000/10,000cells.
多谢大牛赐教!!!! |
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o********r 发帖数: 775 | 45 Review
Many different tumor types have polyclonal tumor origin: Evidence and
implications
Barbara L. ParsonsCorresponding Author Contact Information, a, E-mail The
Corresponding Author
aDivision of Genetic and Reproductive Toxicology, National Center for
Toxicological Research, HFT-120, 3900 NCTR Road, USFDA, Jefferson, AR 72079,
United States
Received 7 February 2008;
revised 14 May 2008;
accepted 15 May 2008.
Available online 31 May 2008.
Abstract
Few ideas have gained such strong acceptance i... 阅读全帖 |
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n********k 发帖数: 2818 | 46 Joke of the day or what?
there is not a most shameless but more shameless!
Results: 33
1.
Viable fertile mice generated from fully pluripotent iPS cells derived from
adult somatic cells.
Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Wang X, Wang L, Zeng F,
Zhou Q.
Stem Cell Rev. 2010 Sep;6(3):390-7.PMID: 20549390 [PubMed - in process]
Related citations
2.
Production of mice using iPS cells and tetraploid complementation.
Zhao XY, Lv Z, Li W, Zeng F, Zhou Q.
Nat Protoc. 2010;5(5):963-71. Epu... 阅读全帖 |
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s****r 发帖数: 736 | 47 这绝对是个大金矿
问题不仅仅局限在DNA/RNA上,还涉及基本代谢,生理表征,繁衍与发育。
最简单的几个问题:
1)由Arsenic构成的DNA/RNA系统,转录翻译有何不同
2)ATP被ATA取代?
3)信号传递系统是否不同?
4)代谢系统,包括酶系统是怎么构成的?
5)细胞如何繁衍?
6)Arsenic 系统和 phosphate 系统之间可否融合?
...
太多太多问题需要解决。
基本上,生命科学进入了新的一章。 |
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m******5 发帖数: 1383 | 48 A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus
1. Felisa Wolfe-Simon1,2,*,
2. Jodi Switzer Blum2,
3. Thomas R. Kulp2,
4. Gwyneth W. Gordon3,
5. Shelley E. Hoeft2,
6. Jennifer Pett-Ridge4,
7. John F. Stolz5,
8. Samuel M. Webb6,
9. Peter K. Weber4,
10. Paul C. W. Davies1,7,
11. Ariel D. Anbar1,3,8 and
12. Ronald S. Oremland2
+ Author Affiliations
1.
1NASA Astrobiology Institute, USA.
2.
2U.S. Geological Survey, Menlo Park, CA, US... 阅读全帖 |
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S******G 发帖数: 965 | 49 “Our data show evidence for arsenate in macromolecules that normally
contain phosphate”
现在看不了全文,有人能说说是什么data吗?有analytical 的化学data吗?
structural biology 自然不必问了。
谢谢 |
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