k*******c
发帖数: 142 | 1 I blocked the membrane overnight at RT. Is it still good for me to continue? |
|
o******d
发帖数: 743 | 2 要租个房子，房东给的租约里有这么一条，说6个月里guests 来访过夜总共超过10天的
话，要房东书面同意。这条有点过了吧？如果跟他们住在一起，我可以理解，我租下整
个房子，偶尔有个访客也要知会他们？加州对overnight guests 怎么规定的？ |
|
l*****a
发帖数: 375 | 3 啥地方的药店需要overnight？觉得美国十点钟以后街上都没多少人了，很难想像有人
会半夜3点来取药。。。摆明了是在等抢啊？
没想明白 谁有空说说？ |
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c*******n
发帖数: 1214 | 4 I have heard that overnight shift in a 24 hour store is quite busy, actually
. You may encounter more fake control rx than the regular hours. Hopefully
LZ can update some information for us. |
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j****0
发帖数: 69 | 5 overnight是太辛苦, 是不是每个药剂师都要轮流值夜班呀? |
|
j****0
发帖数: 69 | 6 that seems resonable.
does overnight get more pay?
off |
|
g******9
发帖数: 13 | 7 在做intraday预测的股票预测的时候，overnight的price jump对模型的估计影响很大
。请问大家有什么好办法调整阿。
或者有这方面的书也可以推荐一下。谢谢！ |
|
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h**********y
发帖数: 41 | 9 谢谢。
我想再追问下，如果我每天都用SGD/USD overnight forward锁定第二天的汇率，30天
之后我到底能不能抵消这30天内汇率波动的风险呢？ |
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w*******y
发帖数: 60932 | 10 Verizon Wireless is offering the VersaLink 7501 802.11b/g Wireless G Router
for $9.97 with Free Overnight Shipping.
Link:
http://www.verizonwireless.com/b2c/store/accessory?action=accessoryDetails&accessoryId=44031
From the Verizon Wireless site:
The Verizon Router delivers high performance WirelessG broadband routing.
Connect to your modem and Netbook, Network Extender, or Verizon Hub and
enjoy:
* Excellent WiFi coverage and throughput
* 4 times more output power than standard 802.11g p |
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w*******y
发帖数: 60932 | 11 Verizon Wireless has the Verizon VZ7501ROUT 802.11g Wireless Router for $10
with free overnight shipping. Your standard wireless G router except for the
fact that its made from Verizon Wireless.
Seems to have an unusually strong wireless signal for such an unassuming
device. Definitely some potential here. 32MB RAM, 8MB flash, but bootloader
still locked, so no third party support from dd-wrt or similar projects.
OTOH, there are several nice hacks under development, including converting
it to a |
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w*******y
发帖数: 60932 | 12 There is a link on the Rachael Ray Home Page that offers Zappos.com VIP
membership for free. That includes free overnight shipping on all orders and
returns.
The show's home page is here;
Link:
http://www.rachaelrayshow.com/
Look on the right side for the large Zappos "friends of the Rachael Ray show
! " section or just try going here directly to register.
Link:
http://vip.zappos.com/friends-of-rachael-ray
Registration was simple and didn't require credit card info.
Now who's Rachael Ray and wha... 阅读全帖 |
|
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w*******y
发帖数: 60932 | 14 Its still not too late for your gifts to reach your loved ones before XMAS.
Amazon is offering free 1 day shipping for the items sold and shipped by
Amazon.
Link:
http://www.amazon.com/gp/promotions/details/AFQLJ36KWR5MJ/
Just add items to cart and select 1 day overnight shipping and all items
should be shipped at same address.
ENJOY....Happy holidays.
|
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w*******y
发帖数: 60932 | 15 Just got this in my email:
You can take advantage of your VIP perks:
-No membership fee. (It's totally free!)
-FREE overnight shipping. (No strings attached!)
-Expedited returns. (Get your money back fast!)
-Exclusive website and phone number.
-VIP Points & Badges.
-Coming Soon: Priority Notifications.
-Plus, bragging rights to the coolest, hippest VIP program on the planet!
Link:
https://secure-vip.zappos.com/promotion/vipRegisterNew/code/nomorewaiting
!
|
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w*******y
发帖数: 60932 | 16 I'm wanting to replace my current clamshell TracFone & am so excited to see
TracFone has finally come out with a couple of very cool phones! I've been
surfing around trying to read up on these but since they are so new I can
only find the excited buzz about them, lol. Both phones seem to offer a lot
of nice features including Double Minutes For Life, MP3 player, camera,
etc.
From now through Mother's Day they will do overnight shipping for free. I
picked up this code on one of the review sit... 阅读全帖 |
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w*******y
发帖数: 60932 | 17 Founded by Gerd R. Lang in 1983 in Karlsfeld, Germany, Chronoswiss is one of
the world's finest watch manufacturers, producing some of the most highly
acclaimed mechanical timepieces. Chronoswiss maintains its exclusivity by
only producing about 7,000 watches per year.
Though a German company, every component is produced in Switzerland and then
the watches are hand-finished. While many of the movements used in
Chronoswiss watches are based on the Enicar 165 movement, the Chronoswiss
versions fea... 阅读全帖 |
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|
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w*******y
发帖数: 60932 | 20 Just pre ordered this bad boy and wanted to share the deal with everyone.
CLICK HERE FOR LINK:
http://www.letstalk.com/samsung-epic-4g-touch-sprint
Phone is already discounted to $100 with pre order and contract signup.
Simply apply coupon code "[COUPON=28418]take20back[/COUPON]" during your
checkout to receive another $20 making this the lowest price for the Galaxy
S on the market. Purchase also includes free overnight shipping for when
phone is released on 9/16/11. This code and discount is wo... 阅读全帖 |
|
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w*******y
发帖数: 60932 | 22 Link:
http://vip.zappos.com/d2/tweet-riddle
Click C for the answer.
It will say that it's closed, but click get your vip status now after that
and you can sign up.
You get free unlimited overnight shipping
|
|
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w*******y
发帖数: 60932 | 24 Net10 has slashed their unlimited plan (Talk, Text, Data) from $50/month to
$30/month. This deal is available on the following phones only
LG 600G - $40
Motorola W408G - $50
Motorola EM326G - $60
Samsung 401G - $70
Fine Print
Must enroll auto-refill within 25 days of activation to get unlimited plan
for $30/month plan for upto 12 months. Free overnight shipping valid until
12/15/2011. Limited time offer. Offer not available in all areas. While
supplies last.
Very likely the data is going to be ... 阅读全帖 |
|
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w*******y
发帖数: 60932 | 26 Saw this while browsing the Dell configure site on AAFES. This is the bundle
that was selling around holidays that included Halo Reach, Fable III, and 3
Month Xbox Live card. Log in AAFES and go to the Dell configure stite.
Click on the now in stores link and its at the very bottom. It also includes
free overnight shipping and of course no taxes as always. Seems like a
decent deal since its selling for $300 every where else.
|
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w*******y
发帖数: 60932 | 27 Amazon has deals on the Pampers Extra Protection Diapers (overnight). If you
check out with Subscribe & Save, Amazon Mom, and use the $1.00 off coupon
on the product page, the prices come out to:
96 count Pampers Extra Protection Diapers (Size 3) - $18.95 ($0.19 per
diaper)
80 count Pampers Extra Protection Diapers (Size 4) - $18.95 ($0.23 per
diaper)
72 count Pampers Extra Protection Diapers (Size 5) - $18.95 ($0.26 per
diaper)
58 count Pampers Extra Protection Diapers (Size 6) - $18.95 ($0.32 ... 阅读全帖 |
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s***l
发帖数: 321 | 28 【 以下文字转载自 Parenting 讨论区 】
发信人: shanl (衫儿), 信区: Parenting
标 题: overnight diapers?
发信站: BBS 未名空间站 (Tue Nov 2 02:08:46 2010, 美东)
一岁两个月男娃
请推荐过夜diaper |
|
r****t
发帖数: 221 | 29 2012年8月2日上午10点正，第六次站在 Mt.Whitney 顶的石堆上，但是第一次把它当作
一段旅程的起点。在这个起点上总共待了15分钟，身体的良好感觉给了我足够的信心。
Big G’s JMT chart is used as distance-measure reference
8月1日（星期三， 晴暖无风）
pickup permit@Eastern Sierra InterAgency Visitor Center, Lone Pine 8:00
A.M.
Whitney Portal. started@Noon.
Lone Pine Lake
Outpost Camp
Trail Camp 3:15 P.M. (stay overnight)
（D: 6Mi， E.G.: 3700FT）
8月2日（星期四， 晴暖无风云）
Trail Camp
Trail Crest 9:00 A.M.
cross@Mt Whitney Trail
Mt Whitney 10:... 阅读全帖 |
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r******h
发帖数: 809 | 30 【 以下文字转载自 Travel 讨论区 】
发信人: rotatezh (剃度在莲台下), 信区: Travel
标 题: 向南一百八十度——巴塔哥尼亚徒步行程和总结
发信站: BBS 未名空间站 (Tue Jan 18 23:54:49 2011, 美东)
累死了，呼呼。这篇针对一般旅行，关于trekking的详细信息，另起一文说明。
背景：wiki上巴塔哥尼亚泛指南纬35到55度，安第斯山两侧，包括智利和阿根廷的部分
领土的一大片区域。从旅游的角度分，巴塔哥尼亚又可以分成几个区，每个区的风景都
很不一样。我们这次主要去了Lake District和South Patanogia两个区。前者主要是阿
尔卑斯风格的雪山湖泊；后者一个是看当地特有的塔峰（Torre），一个是看低海拔峡
谷冰川。
行程：
Day 0: Arrive Bariloche. Overnight @ TangoInn Hostel.
Day 1: Backpack to Refugio Frey, camp.
Day 2: Backpack to Refugio Jakob, camp.
Day 3: Bac... 阅读全帖 |
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r******h
发帖数: 809 | 31 累死了，呼呼。这篇针对一般旅行，关于trekking的详细信息，另起一文说明。
背景：wiki上巴塔哥尼亚泛指南纬35到55度，安第斯山两侧，包括智利和阿根廷的部分
领土的一大片区域。从旅游的角度分，巴塔哥尼亚又可以分成几个区，每个区的风景都
很不一样。我们这次主要去了Lake District和South Patanogia两个区。前者主要是阿
尔卑斯风格的雪山湖泊；后者一个是看当地特有的塔峰（Torre），一个是看低海拔峡
谷冰川。
行程：
Day 0: Arrive Bariloche. Overnight @ TangoInn Hostel.
Day 1: Backpack to Refugio Frey, camp.
Day 2: Backpack to Refugio Jakob, camp.
Day 3: Back to Bariloche. Overnight @ TangoInn Hostel.
Day 4: Morning sightseeing. Afternoon mountain biking. Overnight @ TangoInn.
Day 5: Day... 阅读全帖 |
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s*******t
发帖数: 7746 | 32 肉食动物肉吃太多了，想“改邪归正”，少吃点肉，多吃植物蛋白吧。一个减肥成
功的女同事推荐黑豆（black bean),相对高蛋白底碳水。昨天去买菜就顺便买了一些，超
市里还有各种各样的干豆，比较了一下营养表，发现这个red kidney beans 也是高蛋
白底碳水，也买了一些回家。晚上图方便，把black beans 和red kidney beans 干豆
子洗洗就直接扔slow cooker里煮了，想着第二天早上起床就有豆粥早餐多美好啊。结
果第二天吃了大概1/4 cup，吃完没多久就觉得胃胀气难受，头昏脑胀，想吐却又吐不
出来，难受死了。还有轻微腹泻。 隐约记得吃paleo diet的童鞋们讨论过豆子煮不好
会有毒，放狗一搜，果然。大多数生的豆子里含有一个刺激肠胃的毒素
Phytohaemagglutinin (PHA),一种lectin， 豆类中red kidney beans含lectin量很高,
5个生豆子的毒素足以让人不舒服.这个毒素要高温煮才能破坏，用slow cooker低温煮
最糟糕，把毒素都释放出来，比生的豆子更毒。浑浑噩噩过了几个小时后才好一点, 晚
上吃完... 阅读全帖 |
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P*****a
发帖数: 2464 | 33 想问问去过的或做了research的同学下面的这个行程怎么样，
在www.eoltours.com上看到的，quote是一千美元一个人。
谢谢大家。
Package No # 4L - 9 Nights / 10 Days
(Cairo - Aswan / Luxor Nile Cruise - Alex)
Day 01: Arrival to Cairo airport, meet & assist, then transfer to the ho
tel, overnight.
Day 02: Desert Tour to visit Memphis, Sakkara, Giza Pyramids & Sphinx, i
nclude lunch, overnight.
Day 03: City Tour to explore Egyptian Museum, Saladin Citadel, Coptic ol
d Cairo, old Churches, Jewish Synagogue & Bazaar, include lunch, at even
ing transfer... 阅读全帖 |
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l********7
发帖数: 2648 | 34 我在这边发帖，不是因为他少付我钱；而是为了个人声誉。
我只被风神一个群t过，被踢出去的理由是因为和风神关系不好的神医来往比较密切，
我可以理解。
我除此之外，从没有被其他群t过，请你说话负责人
现在开始写一下我和HMC的事情
第一
我和HMC一共有3笔大交易：
一笔是十月五日的44台 2055d， 当时说好的价格是205.
后来加上了r1400 7台，加起来一共205*44+7*220，
我这边有记录，他已经confirm了， HMC请你说话负责，如果你没有confirm的，我不会
和你要钱。
请注意！！！！！！！！！！！！！
这一笔钱一直都没有还，如果说他后来的付款都是iphone的话。是一直没有还钱
过！！！！！！！！！！！！
第二笔iphone，是十月二十日的27台iphone。
当时，我已经在网上出售，skyglobal给的价格是
895+920；当时还在和HMC一起合作，他手上收了几个32G和64G的想搭着我的一起走， 然后最后给
我的价格是885 915；
我当时和他说好了，当天晚上，他把款付清， 可是你们知道他怎么做的马？
发过去以后，半夜发个帖子，说只付其中一半， 结果... 阅读全帖 |
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x*******1
发帖数: 4787 | 35 4 the benefit of new cop.
【 以下文字转载自 FleaMarket 讨论区 】
发信人: darkeye (不走江湖好久了), 信区: FleaMarket
标 题: Re: yh3000神医， 那460块对你来说很重要吗？
发信站: BBS 未名空间站 (Thu Feb 2 01:51:45 2012, 美东)
考古了一下：
12/20/10：
我收购了yh3000 10个kindle. 单价是$155。 当天给了yh3000 6个label. yh3000分了5
个label给他在UT的朋友（当时告诉我是朋友，后来说其实是护士）。这6个label如下：
FL; 794241023569 (2nd day air)
MD: 794241893372 (2nd day air)
NJ：794241875226 (2nd day air)
NC：796575745881 (overnight)
LA: 794241911320 (2nd day air)
OH: 794241821638 (2nd day air)
from 阅读全帖 |
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Y*****i
发帖数: 601 | 36 【 以下文字转载自 FleaMarket 讨论区 】
发信人: darkeye (不走江湖好久了), 信区: FleaMarket
标 题: Re: yh3000神医， 那460块对你来说很重要吗？
发信站: BBS 未名空间站 (Thu Feb 2 01:51:45 2012, 美东)
考古了一下：
12/20/10：
我收购了yh3000 10个kindle. 单价是$155。 当天给了yh3000 6个label. yh3000分了5
个label给他在UT的朋友（当时告诉我是朋友，后来说其实是护士）。这6个label如下：
FL; 794241023569 (2nd day air)
MD: 794241893372 (2nd day air)
NJ：794241875226 (2nd day air)
NC：796575745881 (overnight)
LA: 794241911320 (2nd day air)
OH: 794241821638 (2nd day air)
from
to 阅读全帖 |
|
m**u
发帖数: 6452 | 37 在flea收货已经有好几年了。和护士的合作基本上都很愉快。虽然出现过问题，经过沟
通和协调，都顺利解决了。我一直觉得自己是很好说话的人。没有想到，还是要留一个
negative feedabck给一个护士: yh3000。
事情的大概经过是这样的。12/20， 我从yh3000手里收购了10个kindle，只有6个被
deliver了，有4个没有被delivery。至今，yh3000拒绝承担任何责任。
事情的详细经过如下：
12/20/10：
我收购了yh3000 10个kindle. 单价是$155。 当天给了yh3000 6个label. yh3000分了5
个label给他在UT的朋友（当时告诉我是朋友，后来说其实是护士）。这6个label如下：
FL; 794241023569 (2nd day air)
MD: 794241893372 (2nd day air)
NJ：794241875226 (2nd day air)
NC：796575745881 (overnight)
LA: 794241911320 (2nd day air)
12/22/10:
我接连收到两个... 阅读全帖 |
|
f*******n
发帖数: 696 | 38 bless
在flea收货已经有好几年了。和护士的合作基本上都很愉快。虽然出现过问题，经过沟
通和协调，都顺利解决了。我一直觉得自己是很好说话的人。没有想到，还是要留一个
negative feedabck给一个护士: yh3000。
事情的大概经过是这样的。12/20， 我从yh3000手里收购了10个kindle，只有6个被
deliver了，有4个没有被delivery。至今，yh3000拒绝承担任何责任。
事情的详细经过如下：
12/20/10：
我收购了yh3000 10个kindle. 单价是$155。 当天给了yh3000 6个label. yh3000分了5
个label给他在UT的朋友（当时告诉我是朋友，后来说其实是护士）。这6个label如下：
FL; 794241023569 (2nd day air)
MD: 794241893372 (2nd day air)
NJ：794241875226 (2nd day air)
NC：796575745881 (overnight)
LA: 794241911320 (2nd day air)
12/22/10:
... 阅读全帖 |
|
c******y
发帖数: 198 | 39 support,护士该罚
在flea收货已经有好几年了。和护士的合作基本上都很愉快。虽然出现过问题，经过沟
通和协调，都顺利解决了。我一直觉得自己是很好说话的人。没有想到，还是要留一个
negative feedabck给一个护士: yh3000。
事情的大概经过是这样的。12/20， 我从yh3000手里收购了10个kindle，只有6个被
deliver了，有4个没有被delivery。至今，yh3000拒绝承担任何责任。
事情的详细经过如下：
12/20/10：
我收购了yh3000 10个kindle. 单价是$155。 当天给了yh3000 6个label. yh3000分了5
个label给他在UT的朋友（当时告诉我是朋友，后来说其实是护士）。这6个label如下：
FL; 794241023569 (2nd day air)
MD: 794241893372 (2nd day air)
NJ：794241875226 (2nd day air)
NC：796575745881 (overnight)
LA: 794241911320 (2nd day air)
12/2... 阅读全帖 |
|
l********7
发帖数: 2648 | 40 【 以下文字转载自 ebiz 讨论区 】
发信人: lucifer007 (LeonZ), 信区: ebiz
标 题: 【郑重申明】HMC请你算账好好算。
发信站: BBS 未名空间站 (Thu Nov 17 23:35:48 2011, 美东)
我在这边发帖，不是因为他少付我钱；而是为了个人声誉。
我只被风神一个群t过，被踢出去的理由是因为和风神关系不好的神医来往比较密切，
我可以理解。
我除此之外，从没有被其他群t过，请你说话负责人
现在开始写一下我和HMC的事情
第一
我和HMC一共有3笔大交易：
一笔是十月五日的44台 2055d， 当时说好的价格是205.
后来加上了r1400 7台，加起来一共205*44+7*220，
我这边有记录，他已经confirm了， HMC请你说话负责，如果你没有confirm的，我不会
和你要钱。
请注意！！！！！！！！！！！！！
这一笔钱一直都没有还，如果说他后来的付款都是iphone的话。是一直没有还钱
过！！！！！！！！！！！！
第二笔iphone，是十月二十日的27台iphone。
当时，我已经在网上出售，skyglobal给的价格是
... 阅读全帖 |
|
d*****e
发帖数: 3868 | 41 考古了一下：
12/20/10：
我收购了yh3000 10个kindle. 单价是$155。 当天给了yh3000 6个label. yh3000分了5
个label给他在UT的朋友（当时告诉我是朋友，后来说其实是护士）。这6个label如下：
FL; 794241023569 (2nd day air)
MD: 794241893372 (2nd day air)
NJ：794241875226 (2nd day air)
NC：796575745881 (overnight)
LA: 794241911320 (2nd day air)
OH: 794241821638 (2nd day air)
from
to
date Mon, Dec 20, 2010 at 3:12 PM
subject Re: wifi kindle 10个 1550
mailed-by gmail.com
hide details 12/20/10
6 labels, more... 阅读全帖 |
|
s***n
发帖数: 9499 | 42 2010的事，原帖被删了？
考古了一下：
12/20/10：
我收购了yh3000 10个kindle. 单价是$155。 当天给了yh3000 6个label. yh3000分了5
个label给他在UT的朋友（当时告诉我是朋友，后来说其实是护士）。这6个label如下：
FL; 794241023569 (2nd day air)
MD: 794241893372 (2nd day air)
NJ：794241875226 (2nd day air)
NC：796575745881 (overnight)
LA: 794241911320 (2nd day air)
OH: 794241821638 (2nd day air)
from
to
date Mon, Dec 20, 2010 at 3:12 PM
subject Re: wifi kindle 10个 1550
mailed-by gmail.com
hide details 12/20/10... 阅读全帖 |
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s****j
发帖数: 2542 | 43 肯尼亚旅游最新攻略制定价格指南
前言- 此行肯尼亚感概甚多，所到之处见国人遍地被宰，付着普遍比欧美游客更高的
价格，让人痛
心疾首 。去肯尼亚之前，我就深感价格非常不透明，每人去的行程不一样，特别难做
参考，于是从我
去的第一天开始，买当地报纸，和当地人交流，观察普通肯尼亚人民的生活物价以及工
资水平，汽油
价格，食品价格，作为此价格指南的基础，希望以后的同胞们以此为基础，拿到合理的
旅行报价。
声明,此文只做参考,个人对自己的行程和取舍负责
1． Safar i camping 和LODGE每日合理报价和利润分析
a. 当地工资水平- 在肯尼亚，除了内罗比，整个国家基本是一个大的野生动物园，
并没有很
多城市，内罗比作为首都，一线城市,如果是在比较好的咖啡厅工作的员工，月工资大
概200USD, 公
司工作的文员大概200USD, 初级的公司技术人员比如会计月薪大概300usd, 在工作3年
和五年之
后，会计大概可以拿到500usd, 大公司的主管级别。以次可以推算一个旅行社的司机，
一天的工资
成本大概也就是10-15usd 左右。
b. 肯尼亚的柴油价格1... 阅读全帖 |
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g*******2
发帖数: 727 | 44 我去年买过一次，1年有效。
价格还好，家里有娃的可以囤几张。
卖的蛮快的。
去年有朋友问我时，这个网站已经卖光了。
今年又开始了。
买到的给个包子啊。。。
http://www.ttia.org/?page=tickets
Discount Tickets from Participating TTIA Member Attractions
Currently available:
SeaWorld San Antonio
$38 adult or child
Six Flags Fiesta Texas (San Antonio)
$31 adult or child
Six Flags Over Texas (Arlington)
$27 adult or child
Hurricane Harbor (Arlington)
$15 adult or child
Space Center Houston
$10 adult or child
Splashtown San Antonio
$14 adult or child
Natural Bridge Caverns (New B... 阅读全帖 |
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t**********c
发帖数: 62 | 45 打算去挪威，基本就是走norway in a nutshell的线路。但是中途希望多住一两天，并
且走一些nin不包括的线路，因为我不太喜欢完全按照标准旅行线路走。
奥斯陆到bergen单程
Day 1: to Oslo (Overnight in Oslo)
Day 2: 游玩Oslo，雕塑公园，皇宫，市政厅，海港，海盗博物馆等 (Overnight in
Oslo)
Day 3: Oslo train to Myrdal, 然后从Myrdal to Flam。这个时候就不继续跟着从
Flam往bergen方向，而是从Flam坐船去Balestrand。据说Balestrand比flam美，而且周
围风景很漂亮(overnight in Balestrand)
Day 4: 从Balestrand跟一个glacier团，一天游（overnight in Balestrand)
Day 5: 从Balestrand坐船到Flam, 然后就继续 Flam to Voss to Bergen (overnight
Bergen)
Day 6: Bergen (overnight Berge... 阅读全帖 |
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t**********c
发帖数: 62 | 46 更改了路线。想去geiranger，和走黄金路线。不知以下路线是否有交通工具可以成行。
Day 1: to Oslo (overnight Oslo)
Day 2: Oslo (overnight Oslo)
Day 3: Oslo-Dombas-Andalsnes-Geiranger (overnight Geiranger) This is the
Golden Route
Day 4: Hurtigruten from Geiranger to Alesund (overnight onboard)
Day 5: Hurtigruten continues from Alesund to Bergen (overnight Bergen)
Day 6: Bergen roundtrip Norway in a Nutshell (overnight Bergen)
Day 7: depart from Bergen |
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c*********r
发帖数: 1312 | 47 多谢啦！Protocol奉上。
我们是用小鼠胚胎的软骨和骨头染色的protocol。染软骨的话只用Alcian Blue即可。
最后胚胎用BABB处理就会变得透明。BABB危险，而且会腐蚀显微镜，使用时要小心。很
多动物的胚胎都可以用类似的方法染骨头（红色）和软骨（蓝色）：鼠，龟，蛇，鸡，
鱼，蜥蜴，变色龙，蝙蝠。。。而且都很漂亮！
比如这个人，做了好多不同动物的染色还到处办展览：
http://www.shinsekai-th.com/en/photo.php
Protocol：
1. Skeletal Preparations- Cartilage and Bone staining
1.1 Cartilage staining in mouse embryos from E12.5 - E13.5
Dissect out embryos, rinse in 1 x PBS, and place in ice-cold 95% Ethanol for
1 hour.
Change to fresh 95% ETOH and rock overnight at room temper... 阅读全帖 |
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T********N
发帖数: 2680 | 48 China's yuan never suffered sharp fall overnight
Rumors swirled on social media on Tuesday that China's currency dropped
sharply in overnight trade, but the purported move may have been an isolated
hiccup from some providers.
Some currency data providers were showing that the yuan tumbled, with the
dollar fetching as much as 7.49 yuan in overnight trade. It wasn't clear if
the data were indicating the offshore yuan or the onshore currency, but the
onshore currency does not trade overnight. That ... 阅读全帖 |
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