G*********o
发帖数: 49669 | 1 national league of makelove。
nlm是啥？
nlm.. |
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P******l
发帖数: 1648 | 2 探讨话题:
3 现今全人类的2号染色体长臂的2q13–2q14.1位置上，都有一段“789个碱基字符的序
列”，它是男人和女人都有的标记。 所有人基本(注意我把我的理解从完全改为基本)
相同 (我们可以称它为“人类DNA的第一个标记”), 这可以作为一个现存人类同源
的证据
for both chromatids
==============
思路过程:
http://www.bydnacoding.org/CHT2-P1.html (这一段是copy paste)
亲子鉴定方法：
常染色体亲子鉴定:
由于常染色体一半来自父亲、一半来自母亲，在常染色体亲子鉴定中，可以确定孩子的
亲生父母。
孩子的每对(两条)常染色体一条来自父亲、一条来自母亲，因此，可以通过比较父、母
和子女在常染色体特定位点上DNA字符代码的序列，来判定亲子关系。
例如，孩子在染色体某个位点上的一串DNA字符代码是“TCAT TCAT TCAT TCAT TCAT
TCAT TCAT TCAT”，这8个“TCAT”的重复，可以看成是一个“遗传标记”。他的母亲
或父亲的DNA，在此位点上一定有这样的8个“TCA... 阅读全帖 |
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f*****5
发帖数: 3 | 4 只要打http://ncbi.nlm.nih.gov 就可以直接联上；
而打ncbi.nlm.nih.gov,就总是联到weblogin,我以前学校的登陆页面(离开很久了，密
码什么早不能用了)。
cookie 什么的应该都清除了，搞不懂，请大拿指教，谢了！ |
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b**********t
发帖数: 366 | 9 1.
Journal: Current drug targets
Title: Vascular Endothelial Growth Factor as an Anti-Angiogenic Target for
Cancer Therapy
Author: Niu, etc
Year 2010, Volume 11, Issue 8, Page 1000-1017
Link:
http://www.ncbi.nlm.nih.gov/pubmed/20426765
2.
Journal: Expert opinion on biological therapy
Title: Oligosaccharides as anti-angiogenic agents
Author: Cole, etc
Year 2008, Volume 8, Issue 3, Page 351-362
Link:
http://www.ncbi.nlm.nih.gov/pubmed/18294105
My email: b********[email protected]
Thanks a lot! :) |
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z***k
发帖数: 33 | 18 挂一漏万，抛砖引玉。
Gilenya/FTY720：免疫抑制剂。临床用于治疗自身免疫性疾病（如硬皮病）。最早由日
本人提取于冬虫夏草。
On September 22, 2010, fingolimod became the first oral disease-modifying
drug approved by the Food and Drug Administration to reduce relapses and
delay disability progression in patients with relapsing forms of multiple
sclerosis. In 2010 and 2011 it was approved for treating multiple sclerosis
in USA/Russia and Europe/Canada/Australia respectively. It is derived from
the myriocin (ISP-1) metabolite of the fungus Isaria sinclai... 阅读全帖 |
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s**u
发帖数: 9035 | 21 1.
Curr Med Chem. 2010;17(36):4427-32.
miRNA: small molecules as potential novel biomarkers in cancer.
Shah AA, Leidinger P, Blin N, Meese E.
Division of Molecular Genetics, Institute of Human Genetics, University of T
übingen, Germany.
http://www.ncbi.nlm.nih.gov/pubmed/21062260
2.
Adv Cancer Res. 2010;108:113-57.
microRNAs in cancer: from bench to bedside.
Cortez MA, Ivan C, Zhou P, Wu X, Ivan M, Calin GA.
Department of Experimental Therapeutics, The University of Texas MD Anderson
Cancer Cent... 阅读全帖 |
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s**u
发帖数: 9035 | 22 1.
Curr Med Chem. 2010;17(36):4427-32.
miRNA: small molecules as potential novel biomarkers in cancer.
Shah AA, Leidinger P, Blin N, Meese E.
Division of Molecular Genetics, Institute of Human Genetics, University of T
übingen, Germany.
http://www.ncbi.nlm.nih.gov/pubmed/21062260
2.
Adv Cancer Res. 2010;108:113-57.
microRNAs in cancer: from bench to bedside.
Cortez MA, Ivan C, Zhou P, Wu X, Ivan M, Calin GA.
Department of Experimental Therapeutics, The University of Texas MD Anderson
Cancer Cent... 阅读全帖 |
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l**********1
发帖数: 5204 | 23 在欧美富不过三代, Thomas R. Cech 诺奖光环也不可能传三代:就看二代徒弟Ming Lei
在美都是只mentor华人博士生和千老的份上, 缺乏脑力潜能资源多样性吧, 海归后清后
更没诺奖光环戏?
www.biochem.med.umich.edu/?q=ming_lei
Ps: 指Ming Lei 作为通讯作者的第一作者Last name 有非华文拼音的吗?
www.ncbi.nlm.nih.gov/pubmed?term=lei+m[au]+telomere
Ming Lei 作为第2作者 通讯作者为他人的不算 i.e.
www.ncbi.nlm.nih.gov/pmc/articles/PMC2490755/?tool=pubmed |
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l**********1
发帖数: 5204 | 25 RE
1.这回你Microtus (田鼠啦啦啦) 误解了,
>关于你的第一个问题，pi和pd，学生的关系，你的意思是不是如果pd，学生过的好一
些，
----------------'
我是说12年大选如果共和党的总统上台把NIH的90% R01/K99 Cut了以后, 让那些还米美
国绿卡的破四刀咋办? 是跳和谐Shi YG or RY PI那里呢还是回其她大陆的国家那里去
当pi奴隶啊? 原始丛林社会中不讲究EQ 只重视CFQ=Catch Food Q 当然需要IQ去竞
争获得的.
*******************
2
当然当然好的收入容易出学术成果但是如果考虑annual收入多少USD/ 比裴多非的生
命,爱情, 自由还要重的话, 伽罗瓦还会書出伽罗瓦群論吗? 阿贝尔还会書出阿贝尔群
吗? 今天生物工程数学领域还用到这些群論Multi Scale Complex systems biology:
please refer
Gerhard Werner
Fractals in the Nervous System: Conceptual Implications fo... 阅读全帖 |
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l**********1
发帖数: 5204 | 26 So what's up next cutting edge MS proteomics method?
ZZ
Why nanoLiters?
Nanoliter Cool Wave®
exciting, inexpensive, sample handling platform & technology.
Booth 17 Improve sensitivity of MALDI, DART, DESI, SIMS, LDI 10 to 100x
LITERALLY!
Also, manipulate and spatially, temporally concentrate samples for TLC, MS,
IR, NMR, PCR; electrophoresis, fluorescence, crystallization & more.
Dispense viscous liquids non-touch (glycerol, blood, serum, ionic liqs.,
glues) for forensics, defense, medical,... 阅读全帖 |
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z**********8
发帖数: 766 | 30 1) http://www.ncbi.nlm.nih.gov/pubmed/22082758
Semin Oncol. 2011 Dec;38(6):724-33.
MicroRNAs in the pathogenesis of cancer.
Lovat F, Valeri N, Croce CM.
Molecular Virology Immunology & Medical Genetics, The Ohio State University
Comprehensive Cancer Center, Columbus, OH 43210, USA.
2) http://www.ncbi.nlm.nih.gov/pubmed/22082760
Semin Oncol. 2011 Dec;38(6):743-51.
MicroRNAs in mutagenesis, genomic instability, and DNA repair.
Landau DA, Slack FJ.
Department of Hematology, Yale University School o... 阅读全帖 |
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l**********1
发帖数: 5204 | 31 So Plos Biology permit three Co-first-authoship even not post-genome/trans
proteomics report of one major model species likes below 2002 Science
htp://www.ncbi.nlm.nih.gov/pubmed/11935017
or 2009 PNAS
htp://www.ncbi.nlm.nih.gov/pubmed/19372371
jinglingzi (JingLingZi) 的大作中提到:
htp://www.mitbbs.com/article_t/Biology/31624531.html |
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l**********1
发帖数: 5204 | 32 De rien.
Plus another one paper:
htp://www.ncbi.nlm.nih.gov/pubmed/22203841
>Grey scale images are generated, reproducing the fluo- rescence intensity
in different tissue components (Figure 1). The estimated resolution is 0.5
μm in lateral direction and 1- 2 μm in the axial direction
full text link:
htp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235701/pdf/DRP2012-810749.pdf
if LZ can not recognize 200 μm scale labeled GFP particle color signal just try at least 30 μm or more sensitive
likes above ... 阅读全帖 |
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l**********1
发帖数: 5204 | 33 if transfer to mice
pls go to
htp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791161/
its Fig. 3B
and if transfer to cell lines
htp://www.ncbi.nlm.nih.gov/pubmed/22134125 |
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l**********1
发帖数: 5204 | 34 Pls go to
httq://www.ncbi.nlm.nih.gov/pubmed/21926998
NB: http now is "httq" from some bug might happened while using direct URL
address here.
if FAQ (Just F A Q) 的 target scale is below 15nm i.e. virus likes HSV
now your only choice is SEM not fluorescence microscopy.
pls refer
2011 paper:
httq://www.ncbi.nlm.nih.gov/pubmed/21345968
Ps:
这里也是现炒现卖 没有楼上的提示
还得不到
科普 偶本人 和whom want to know sth about size scale:
30 nm Small virus (Picornaviruses)
httq://en.wikibooks.org/wiki/Cell_Biology/Introducti... 阅读全帖 |
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l**********1
发帖数: 5204 | 35 not only Biology but Chemistry:
please go to
//www.ncbi.nlm.nih.gov/pubmed/20929839
or
//www.ncbi.nlm.nih.gov/pubmed/21310963 |
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l**********1
发帖数: 5204 | 36 LZ please try below (I) to (IV) four strands overlap PCR
your target 7kp=2kp(I)+2kp(II)+2kp(III)+1kp(IV)
if you did not know downstream any conservative sequence information
your 3' RACE can use ligation-anchored PCR
就是用内切酶
//en.wikipedia.org/wiki/Restriction_enzyme
对应的人工引物锚定long mRNA 的3'端 做人工锚定引物
i.e. by NotI it should be inserted to 3'端 5'GC---GGCCGC
from
//en.wikipedia.org/wiki/Restriction_enzyme
Enzyme Source Recognition Sequence Cut
NotI Nocardia otitidis
5'GCGGCCGC
3'CGCCGG... 阅读全帖 |
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l**********1
发帖数: 5204 | 37 让你老板买个半自动或全自动工作站做Y-2-H 不就得拉
或借用同校里的其它单元的那设备
没听说过欧美高通量Y-2-H 筛选用一双手做的
半自动工作站 96 well
please below link:
//www.tecan.com/platform/content/element/5135/Tecan_Journal_09_01.pdf
for one
France developed a semi- automated yeast two-hybrid system with the Te-
MOTM 96 Multichannel Pipetting Option from Tecan, halving the time taken to
obtain reproducible results from six to three days.
if try Y-1-H
全自动工作站 384 well
please refer last Oct. Nature Methods two papers:
//www.ncbi.nlm.nih.gov/pubmed/22037705
/... 阅读全帖 |
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l**********1
发帖数: 5204 | 38 Please try refer
if could do 2D/3D QSAR Quantitative structure–activity relationship tool to
your N protein with B
papers link:
//www.ncbi.nlm.nih.gov/pmc/articles/PMC3141047/
or
//www.ncbi.nlm.nih.gov/pubmed/20726601
----
other approach:
Do N X-ray 3D structure analysis if PDB bank without its record
Normal Protocol process:
please go to
//uit.no/publikum/prosjekter/prosjekt?p_document_id=228973 (QSAR)
For information services and faciities, please follow the links below.
SP1 Protein productio... 阅读全帖 |
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l**********1
发帖数: 5204 | 39 COLD-PCR then Agarose Gel electrophoresis
Abstract
The detection of low-abundance DNA variants or mutations is of particular interest to medical diagnostics,
individualized patient treatment and cancer prognosis; however, detection sensitivity for low-abundance
variants is a pronounced limitation of most currently available molecular assays. We have recently
developed coamplification at lower denaturation temperature-PCR (COLD-PCR) to resolve this limitation.
//www.ncbi.nlm.nih.gov/pmc/articles... 阅读全帖 |
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l**********1
发帖数: 5204 | 41 Bingo
plus 病毒proteins ICP0 to remodel viral chromatin Circadian CLOCK
PNAS 2010
web link:
//www.ncbi.nlm.nih.gov/pubmed/20876123
and J Virol. (2011) web link:
//www.ncbi.nlm.nih.gov/pubmed/21734043 |
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l**********1
发帖数: 5204 | 42 plus
2005 Science review:
//www.ncbi.nlm.nih.gov/pubmed/16109872
and above review 1st author her lab:
//www.life.illinois.edu/clockworks/pages/Martha%20U.%20Gillette.html
and one Science 2011 paper shown fly sleep manipulation.
//www.ncbi.nlm.nih.gov/pubmed/21700877
lol |
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l**********1
发帖数: 5204 | 43 细菌 贫营养的状态 若其lac operon进入 1/3 subharmonic oscillatory 状态的话 就是细菌的睡觉吧
具体参看下边的Goodwin BC 1965 paper
Introducing mutual coupling between the oscillators, with klz = 1.2, km = 0.
4, a phenomenon known as
subharmonic resonance is observed as shown in Fig. 9. Both variables now
have the same frequency, which is considerably smaller than either of the
free-running frequencies. The lower curve is Y~, and in it one can still see
the
original frequency on which has been superimposed the slower frequency of... 阅读全帖 |
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l**********1
发帖数: 5204 | 44 please go to
//www.ncbi.nlm.nih.gov/pubmed/20228280
or
//www.ncbi.nlm.nih.gov/pubmed/18977028 |
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l**********1
发帖数: 5204 | 45 Why only think that positive aspects from noise in biology 这个可以连接或
者引出好几个问题specificity, phenotypic
..
please go to
//www.ncbi.nlm.nih.gov/pubmed/21737735
//www.eng.cam.ac.uk/news/stories/2011/cellular_noise/
//www.ncbi.nlm.nih.gov/pubmed/20829788
..
？ |
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l**********1
发帖数: 5204 | 46 De rien.
plus
please go to
//www.ncbi.nlm.nih.gov/pubmed/22198840
for
The planarity of peptide bonds is an assumption that underlies decades of theoretical modeling of proteins
PNAS 2012 paper
but
Nonplanar peptide bonds in proteins are common and conserved but not biased toward active sites.
by Berkholz DS, Driggers CM, Shapovalov MV, Dunbrack RL Jr, Karplus PA.
Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):449-
and above paper 1st author his PubMed total records:
//www.ncbi.nlm.nih.gov/pubmed?... 阅读全帖 |
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l**********1
发帖数: 5204 | 47 Why only think that positive aspects from noise in biology 这个可以连接或
者引出好几个问题specificity, phenotypic
..
please go to
//www.ncbi.nlm.nih.gov/pubmed/21737735
//www.eng.cam.ac.uk/news/stories/2011/cellular_noise/
//www.ncbi.nlm.nih.gov/pubmed/20829788
..
？ |
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l**********1
发帖数: 5204 | 48 please go to
//www.gwascentral.org/studies?
q=&t=ZERO&m=All&page=1&format=&page_size=50&o=Number+of+markers+(descending)
and
//www.ncbi.nlm.nih.gov/pubmed/19740415
or
//www.ncbi.nlm.nih.gov/pubmed/21158748 |
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l**********1
发帖数: 5204 | 49 if your target mRNA its 3' UTR super long (over several kp) tor before the stop codon high GC rich region
then you can only get with normal qRTPCR then PCR whole 3RACE PCR products.
This case please try another method:
: ligation-anchored PCR cloning
一句话就是用内切酶对应的人工引物锚定: 3'端 做人工锚定引物PCR
mRNA with a primer (3'-poly
(dT)17 with an anchor sequence at its 5'end, then qRTPCR then PCR sub cloning... sequencing.
References:
//genome.cshlp.org/content/4/1/19.full.pdf
//www.ncbi.nlm.nih.gov/pmc/articles... 阅读全帖 |
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l**********1
发帖数: 5204 | 50 DYRead from
//download.nccr-nano.org/study/master/thesis_archive/2009UrbaniRaphael.pdf
//www.ncbi.nlm.nih.gov/pmc/articles/PMC3062589/
//www.ncbi.nlm.nih.gov/pubmed/11059483 |
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